Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,104,605	T→C	V58A (GTG→GCG)	mltC →	membrane‑bound lytic murein transglycosylase C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,104,605	0	T	C	100.0%	250.3 / NA	70	V58A (GTG→GCG)	mltC	membrane‑bound lytic murein transglycosylase C
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (38/32);  total (38/32)

TTGCTCATCTCCTGTTCGACGACCAAAAAAGGCGATACCTATAACGAAGCCTGGGTCAAAGATACCAACGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGTGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAAT  >  NC_000913/3104469‑3104740                                                                                                                                         |                                                                                                                                        ttGCTCATCTCCTGTTCGACGACCAAAAAAGGCGATACCTATAACGAAGCCTGGGTCAAAGATACCAACGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGAt                                                                                                                                      >  2:349928/1‑140 (MQ=255)  tGCTCATCTCCTGTTCGACGACCAAAAAAGGCGATACCTATAACGAAGCCTGGGTCAAAGATACCAACGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATc                                                                                                                                     >  2:68869/1‑140 (MQ=255)   gCTCATCTCCTGTTCGACGACCAAAAAAGGCGATACCTATAACGAAGCCTGGGTCAAAGATACCAACGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATCg                                                                                                                                    >  1:1160545/1‑140 (MQ=255)    cTCATCTCCTGTTCGACGACCAAAAAAGGCGATACCTATAAAGAAGACTGGGTAAAAGATGCCAACGCTTTTGATATTCTGATGGGGCAATTTGACCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATCGc                                                                                                                                   <  2:1226512/140‑1 (MQ=255)      cATCTCCTGTTCGACGACCAAAAAAGGCGATACCTATAACGAAGCCTGGGTCAAAGATACCAACGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATCGCTg                                                                                                                                 <  2:435631/140‑1 (MQ=255)      cATCTCCTGTTCGACGACCAAAAAAGGCGATACCTATAACGAAGCCTGGGTCAAAGATACCAACGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATCGCTg                                                                                                                                 <  2:552108/140‑1 (MQ=255)      cATCTCCTGTTCGACGACCAAAAAAGGCGATACCTATAACGAAGCCTGGGTCAAAGATACCAACGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATCGCTg                                                                                                                                 <  2:1186062/140‑1 (MQ=255)      cATCTCCTGTTCGACGACCAAAAAAGGCGATACCTATAACGAAGCCTGGGTCAAAGATACCAACGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATCGCTg                                                                                                                                 <  1:106494/140‑1 (MQ=255)         ctcCTGTTCGACGACCAAAAAAGGCGATACCTATAACGAAGCCTGGGTCAAAGATACCAACGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATCGCTg                                                                                                                                 >  2:342880/1‑137 (MQ=255)         ctcCTGTTCGACGACCAAAAAAGGCGATACCTATAACGAAGCCTGGGTCAAAGATACCAACGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATCGCTg                                                                                                                                 <  1:342880/137‑1 (MQ=255)         ctcCTGTTCGACGACCAAAAAAGGCGATACCTATAACGAAGCCTGGGTCAAAGATACCAACGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATCGCTGGTc                                                                                                                              >  2:232281/1‑140 (MQ=255)              gTTCGACGACCAAAAAAGGCGATACCTATAACGAAGCCTGGGTCAAAGATACCAACGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATCGCTg                                                                                                                                 <  2:276079/132‑1 (MQ=255)              gTTCGACGACCAAAAAAGGCGATACCTATAACGAAGCCTGGGTCAAAGATACCAACGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATCGCTg                                                                                                                                 >  1:276079/1‑132 (MQ=255)                  gacgacCAAAAAAGGCGATACCTATAACGAAGCCTGGGTCAAAGATACCAACGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATCGCTGGTCCTAAGGACt                                                                                                                     <  1:232281/140‑1 (MQ=255)                  gacgacCAAAAAAGGCGATACCTATAACGAAGCCTGGGTCAAAGATACCAACGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATCGCTGGTCCTAAGGACt                                                                                                                     <  1:68869/140‑1 (MQ=255)                    cgacCAAAAAAGGCGATACCTATAACGAAGCCTGGGTCAAAGATACCAACGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTAc                                                                                                                   <  1:705334/140‑1 (MQ=255)                      acCAAAAAAGGCGATACCTATAACGAAGCCTGGGTCAAAGATACCAACGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGt                                                                                                                 >  1:838506/1‑140 (MQ=255)                         aaaaaaGGCGATACCTATAACGAAGCCTGGGTCAAAGATACCAACGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGTGaa                                                                                                              >  1:1009242/1‑140 (MQ=255)                              aGGCGATACCTATAACGAAGCCTGGGTCAAAGATACCAACGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGTGAAATaca                                                                                                         <  1:83235/140‑1 (MQ=255)                              aGGCGATACCTATAACGAAGCCTGGGTCAAAGATACCAACGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGTGAAATaca                                                                                                         <  2:487302/140‑1 (MQ=255)                               ggCGATACCTATAACGAAGCCTGGGTCAAAGATACCAACGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGTGAAATacac                                                                                                        <  1:349928/140‑1 (MQ=255)                                 cGATACCTATAACGAAGCCTGGGTCAAAGATACCAACGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGTGAAATACACCg                                                                                                      >  1:70890/1‑140 (MQ=255)                                               aaGCCTGGGTCAAAGATACCAACGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGAcc                                                                                        <  2:1008293/140‑1 (MQ=255)                                                aGCCTGGGTCAAAGATACCAACGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGAccc                                                                                       >  1:438839/1‑140 (MQ=255)                                                aGCCTGGGTCAAAGATACCAACGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGAccc                                                                                       >  1:272158/1‑140 (MQ=255)                                                 gCCTGGGTCAAAGATACCAACGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGTGAAATacac                                                                                                        <  2:896672/122‑1 (MQ=255)                                                 gCCTGGGTCAAAGATACCAACGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGTGAAATacac                                                                                                        >  1:896672/1‑122 (MQ=255)                                                 gCCTGGGTCAAAGATACCAACGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCg                                                                                      >  1:524529/1‑140 (MQ=255)                                                 gCCTGGGTCAAAGATACCAACGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCg                                                                                      >  1:78048/1‑140 (MQ=255)                                                  ccTGGGTCAAAGATACCAACGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGc                                                                                     >  1:1030242/1‑140 (MQ=255)                                                   cTGGGTCAAAGATACCAACGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCa                                                                                    <  2:524529/140‑1 (MQ=255)                                                      ggTCAAAGATACCAACGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGcc                                                                                 >  2:1211646/1‑140 (MQ=255)                                                      ggTCAAAGATACCAACGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGGGCTGCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGcc                                                                                 >  2:857519/1‑140 (MQ=255)                                                                aCCAACGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACtt                                                                       >  1:511603/1‑140 (MQ=255)                                                                   aaCGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGTGAAATACGCCGATCAATATCAGACCCGCAGCCACATCAACTTCGa                                                                    <  2:838506/140‑1 (MQ=255)                                                                    aCGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGAt                                                                   <  1:857519/140‑1 (MQ=255)                                                                    aCGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGAt                                                                   >  2:206532/1‑140 (MQ=255)                                                                    aCGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGAt                                                                   <  2:78048/140‑1 (MQ=255)                                                                    aCGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGAt                                                                   <  2:438839/140‑1 (MQ=255)                                                                     cGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATg                                                                  <  2:1160545/140‑1 (MQ=255)                                                                              tatTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGa                                                         <  1:206532/140‑1 (MQ=255)                                                                                ttCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGAtt                                                       >  2:999281/1‑140 (MQ=255)                                                                                 tCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTa                                                      <  1:999281/140‑1 (MQ=255)                                                                                  cTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTAc                                                     >  2:592460/1‑140 (MQ=255)                                                                                     aTGGGGCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTAt                                                  >  1:617159/1‑140 (MQ=255)                                                                                       ggggCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTa                                                            <  2:386420/128‑1 (MQ=255)                                                                                       ggggCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTa                                                            >  1:386420/1‑128 (MQ=255)                                                                                       ggggCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCg                                                >  1:576291/1‑140 (MQ=255)                                                                                        gggCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGa                                               >  2:684856/1‑140 (MQ=255)                                                                                            aaTTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAAcc                                           <  1:335857/140‑1 (MQ=255)                                                                                            aaTTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAAcc                                           <  1:684856/140‑1 (MQ=255)                                                                                             aTTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAActa                                          <  2:1009242/140‑3 (MQ=255)                                                                                             aTTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCa                                          >  1:200393/1‑140 (MQ=255)                                                                                               ttGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATc                                        >  1:369507/1‑140 (MQ=255)                                                                                                 gCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGc                                      <  2:369507/140‑1 (MQ=255)                                                                                                 gCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGc                                      <  2:576291/140‑1 (MQ=255)                                                                                                                aaCATCTGGGGCTTCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGc                       >  1:777812/1‑140 (MQ=255)                                                                                                                 aCATCTGGGGCTTCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGcc                      >  1:1034723/1‑140 (MQ=255)                                                                                                                 aCATCTGGGGCTTCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGcc                      >  2:247111/1‑140 (MQ=255)                                                                                                                  cATCTGGGGCTTCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCcg                     <  2:924018/140‑1 (MQ=255)                                                                                                                     cTGGGGCTTCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCgcgc                  >  1:60219/1‑140 (MQ=255)                                                                                                                     cTGGGGCTTCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCgcgc                  >  1:258969/1‑140 (MQ=255)                                                                                                                     cTGGGGCTTCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCgcgc                  >  1:234383/1‑140 (MQ=255)                                                                                                                     cTGGGGCTTCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATAGAAACCATCGCCGCGACAGCACCTGCCgcgc                  >  2:681427/1‑140 (MQ=255)                                                                                                                       ggggCTTCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCAt                <  2:200393/140‑1 (MQ=255)                                                                                                                        gggCTTCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCAtt               >  1:778530/1‑140 (MQ=255)                                                                                                                            ttCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTgcg           <  2:511603/140‑1 (MQ=255)                                                                                                                             tCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTgcgc          >  2:356092/1‑140 (MQ=255)                                                                                                                             tCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTgcgc          >  2:64303/1‑140 (MQ=255)                                                                                                                             tCAAAGAGGTGGCGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTgcgc          >  2:275573/1‑140 (MQ=255)                                                                                                                                    ggtggcGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCaa   >  2:1177948/1‑140 (MQ=255)                                                                                                                                     gtggcgATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAAt  >  2:925742/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        TTGCTCATCTCCTGTTCGACGACCAAAAAAGGCGATACCTATAACGAAGCCTGGGTCAAAGATACCAACGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGTGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAAT  >  NC_000913/3104469‑3104740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑