Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,126,460	A→G	D21D (GAT→GAC)	glcE ←	glycolate dehydrogenase, putative FAD‑binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,126,460	0	A	G	100.0%	288.9 / NA	79	D21D (GAT→GAC)	glcE	glycolate dehydrogenase, putative FAD‑binding subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (40/39);  total (40/39)

CGCGCGGTTATCACCAGCTCGGTCGGGTCGTAATTAACAATGCCGCGATGACAACGAACATCCAGCGTTTGCCCGGTGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTATCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAGGGAAAGGTAAATGACCGTGATGCACATGCATGGCACCAAATTCAGCACAGC  >  NC_000913/3126326‑3126592                                                                                                                                       |                                                                                                                                     cgcgcgGTTATCACCAGCTCGGTCGGGTCGTAATTAACAATGCCGCGATGACAACGAACATCCAGCGTTTGCCCGGTGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCt                                                                                                                                 >  2:706077/1‑140 (MQ=255) cgcgcgGTTATCACCAGCTCGGTCGGGTCGTAATTAACAATGCCGCGATGACAACGAACATCCAGCGTTTGCCCGGTGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCt                                                                                                                                 >  2:855278/1‑140 (MQ=255)   cgcgGTTATCACCAGCTCGGTCGGGTCGTAATTAACAATGCCGCGATGACAACGAACATCCAGCGTTTGCCCGGTGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTaa                                                                                                                               >  1:670080/1‑140 (MQ=255)   cgcgGTTATCACCAGCTCGGTCGGGTCGTAATTAACAATGCCGCGATGACAACGAACATCCAGCGTTTGCCCGGTGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTaa                                                                                                                               <  2:343901/140‑1 (MQ=255)   cgcgGTTATCACCAGCTCGGTCGGGTCGTAATTAACAATGCCGCGATGACAACGAACATCCAGCGTTTGCCCGGTGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTaa                                                                                                                               <  1:855278/140‑1 (MQ=255)    gcgGTTATCACCAGCTCGGTCGGGTCGTAATTAACAATGCCGCGATGACAACGAACATCCAGCGTTTGCCCGGTGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAAt                                                                                                                              <  2:581163/140‑1 (MQ=255)    gcgGTTATCACCAGCTCGGTCGGGTCGTAATTAACAATGCCGCGATGACAACGAACATCCAGCGTTTGCCCGGTGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAAt                                                                                                                              <  2:397923/140‑1 (MQ=255)               cAGCTCGGTCGGGTCGTAATTAACAATGCCGCGATGACAACGAACATCCAGCGTTTGCCCGGTGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCa                                                                                                                   <  1:37513/140‑1 (MQ=255)                aGCTCGGTCGGGTCGTAATTAACAATGCCGCGATGACAACGAACATCCAGCGTTTGCCCGGTGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCAc                                                                                                                  >  1:656035/1‑140 (MQ=255)                 gCTCGGTCGGGTCGTAATTAACAATGCCGCGATGACAACGAACATCCAGCGTTTGCCCGGTGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCAcc                                                                                                                 <  2:233950/140‑1 (MQ=255)                  cTCGGTCGGGTCGTAATTAACAATGCCGCGATGACAACGAACATCCAGCGTTTGCCCGGTGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCt                                                                                                                >  1:24334/1‑140 (MQ=255)                  cTCGGTCGGGTCGTAATTAACAATGCCGCGATGACAACGAACATCCAGCGTTTGCCCGGTGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCt                                                                                                                >  1:218136/1‑140 (MQ=255)                  cTCGGTCGGGTCGTAATTAACAATGCCGCGATGACAACGAACATCCAGCGTTTGCCCGGTGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCt                                                                                                                >  2:221810/1‑140 (MQ=255)                  cTCGGTCGGGTCGTAATTAACAATGCCGCGATGACAACGAACATCCAGCGTTTGCCCGGTGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCt                                                                                                                >  1:912825/1‑140 (MQ=255)                  cTCGGTCGGGTCGTAATTAACAATGCCGCGATGACAACGAACATCCAGCGTTTGCCCGGTGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCt                                                                                                                >  1:916315/1‑140 (MQ=255)                  cTCGGTCGGGTCGTAATTAACAATGCCGCGATGACAACGAACATCCAGCGTTTGCCCGGTGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCt                                                                                                                >  1:493285/1‑140 (MQ=255)                  cTCGGTCGGGTCGTAATTAACAATGCCGCGATGACAACGAACATCCAGCGTTTGCCCGGCGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCAcc                                                                                                                 <  2:791322/139‑1 (MQ=255)                  cTCGGTCGGGTCGTAATTAACAATGCCGCGATGACAACGAACATCCAGCGTTTGCCCGGCGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCAcc                                                                                                                 >  1:791322/1‑139 (MQ=255)                     ggtcgggtcgTAATTAACAATGCCGCGATGACAACGAACATCCAGCGTTTGCCCGGTGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCt                                                                                                             >  1:455325/1‑140 (MQ=255)                      gtcgggtcgTAATTAACAATGCCGCGATGACAACGAACATCCAGCGTTTGCCCGGTGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTc                                                                                                            >  2:171935/1‑140 (MQ=255)                           gtcgTAATTAACAATGCCGCGATGACAACGAACATCCAGCGTTTGCCCGGTGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATACACCTGCTCcagca                                                                                                       <  2:916315/140‑1 (MQ=255)                                aaTTAACAATGCCGCGATGACAACGAACATCCAGCGTTTGCCCGGTGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGcc                                                                                                  <  2:912825/140‑1 (MQ=255)                                 aTTAACAATGCCGCGATGACAACGAACATCCAGCGTTTGCCCGGTGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAgcg                                                                                                    >  1:945944/1‑137 (MQ=255)                                 aTTAACAATGCCGCGATGACAACGAACATCCAGCGTTTGCCCGGTGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAgcg                                                                                                    <  2:945944/137‑1 (MQ=255)                                  ttAACAATGCCGCGATGACAACGAACATCCAGCGTTTGCCCGGTGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTg                                                                                                <  1:706077/140‑1 (MQ=255)                                   tAACAATGCCGCGATGACAACGAACATCCAGCGTTTGCCCGGTGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTgg                                                                                               <  2:656035/140‑1 (MQ=255)                                        aTGCCGCGATGACAACGAACATCCAGCGTTTGCCCGGTGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTGGCTGTa                                                                                          >  1:1165536/1‑140 (MQ=255)                                         tGCCGCGATGACAACGAACATCCAGCGTTTGCCCGGTGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCcagca                                                                                                       <  2:1143562/126‑1 (MQ=255)                                         tGCCGCGATGACAACGAACATCCAGCGTTTGCCCGGTGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCcagca                                                                                                       >  1:1143562/1‑126 (MQ=255)                                         tGCCGCGATGACAACGAACATCCAGCGTTTGCCCGGTGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTGGCTGTaa                                                                                         >  2:928553/1‑140 (MQ=255)                                         tGCCGCGATGACAACGAACATCCAGCGTTTGCCCGGTGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTGGCTGTaa                                                                                         >  1:130514/1‑140 (MQ=255)                                         tGCCGCGATCACAACGAACATCCAGCGTTTGCCCGGTGACAGCGCGACCTAAAAAGGCTTTGCTATTTCTGGCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCGGCCTGTaa                                                                                         >  2:1226357/1‑140 (MQ=255)                                          gCCGCGATGACAACGAACATCCAGCGTTTGCCCGGTGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAgcg                                                                                                    >  1:1174196/1‑128 (MQ=255)                                          gCCGCGATGACAACGAACATCCAGCGTTTGCCCGGTGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAgcg                                                                                                    <  2:1174196/128‑1 (MQ=255)                                          gCCGCGATGACAACGAACATCCAGCGTTTGCCCGGTGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTGGCTGTAAt                                                                                        <  2:218802/140‑1 (MQ=255)                                            cgcgATGACAACGAACATCCAGCGTTTGCCCGGTGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTGGCTGTAATca                                                                                      >  2:314515/1‑140 (MQ=255)                                              cgATGACAACGAACATCCAGCGTTTGCCCGGTGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTGGCTGTAATcaca                                                                                    <  1:393242/140‑1 (MQ=255)                                               gATGACAACGAACATCCAGCGTTTGCCCGGTGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTGGCTGTAATCacac                                                                                   <  2:39005/140‑1 (MQ=255)                                                     aaCGAACATCCAGCGTTTGCCCGGTGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTGGCTGTAATCACACTCGCGt                                                                             <  2:130514/140‑1 (MQ=255)                                                     aaCGAACATCCAGCGTTTGCCCGGTGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTGGCTGTAATCACACTCGCGt                                                                             <  2:1165536/140‑1 (MQ=255)                                                     aaCGAACATCCAGCGTTTGCCCGGTGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTGGCTGTAATCACACTCGCGt                                                                             <  1:928553/140‑1 (MQ=255)                                                          aCATCCAGCGTTTGCCCGGTGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTGGCTGTAATCACACTCGCGTAGCAt                                                                        >  2:22840/1‑140 (MQ=255)                                                               cAGCGTTTGCCCGGTGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGaa                                                                   >  2:791617/1‑140 (MQ=255)                                                               cAGCGTTTGCCCGGTGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGaa                                                                   >  1:460329/1‑140 (MQ=255)                                                                aGCGTTTGCCCGGTGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGaaa                                                                  >  2:133766/1‑140 (MQ=255)                                                                  cGTTTGCCCGGTGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACg                                                                >  1:680829/1‑140 (MQ=255)                                                                    tttGCCCGGTGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCt                                                              >  2:503718/1‑140 (MQ=255)                                                                     ttGCCCGGTGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTc                                                             <  1:171935/140‑1 (MQ=255)                                                                     ttGCCCGGTGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTc                                                             <  1:22840/140‑1 (MQ=255)                                                                     ttGCCCGGTGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTc                                                             <  2:493285/140‑1 (MQ=255)                                                                     ttGCCCGGTGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTc                                                             <  2:455325/140‑1 (MQ=255)                                                                      tGCCCGGTGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTcc                                                            >  1:664886/1‑140 (MQ=255)                                                                         ccGGTGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGt                                                         <  2:166227/140‑1 (MQ=255)                                                                               aCAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAggg                                                   <  1:133766/140‑1 (MQ=255)                                                                                  gggCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAGGGaaa                                                >  2:1019538/1‑140 (MQ=255)                                                                                   ggCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAGGGAAAg                                               >  2:969065/1‑140 (MQ=255)                                                                                       aCCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAGGGaa                                                 >  2:960912/1‑134 (MQ=255)                                                                                       aCCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAGGGaa                                                 <  1:960912/134‑1 (MQ=255)                                                                                       aCCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAGGGAAAGGTaa                                           >  1:407510/1‑140 (MQ=255)                                                                                       aCCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAGGGAAAGGTaa                                           >  1:305239/1‑140 (MQ=255)                                                                                              aaGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAGGGAAAGGTAAATGACCg                                    <  1:791617/140‑1 (MQ=255)                                                                                              aaGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAGGGAAAGGTAAATGACCg                                    <  1:1019538/140‑1 (MQ=255)                                                                                                ggCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAGGGAAAGGTAAATGACCGTg                                  <  2:100255/140‑1 (MQ=255)                                                                                                  cTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAGGGAAAGGTAAATGACCg                                    >  1:393822/1‑136 (MQ=255)                                                                                                  cTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAGGGAAAGGTAAATGACCg                                    <  2:393822/136‑1 (MQ=255)                                                                                                   tttGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAGGGAAAGGTAAATGACCGTGATg                               >  1:496729/1‑140 (MQ=255)                                                                                                    ttGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAGGGAAAGGTAAATGACCGTGATGc                              >  1:735701/1‑140 (MQ=255)                                                                                                               gcCCTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAGGGAAAGGTAAATGACCGTGATGCACATGCATGGc                   >  2:110927/1‑140 (MQ=255)                                                                                                                cccTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCATCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAGGGAAAGGTAAATGACCGTGATGCAcatgcat                      >  2:516700/1‑136 (MQ=255)                                                                                                                cccTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCATCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAGGGAAAGGTAAATGACCGTGATGCAcatgcat                      <  1:516700/136‑1 (MQ=255)                                                                                                                cccTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAGGGAAAGGTAAATGACCGTGATGCACATGCATGGCa                  <  2:407510/140‑1 (MQ=255)                                                                                                                  cTGAATCACCAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAGGGAAAGGTAAATGACCGTGATGCACATGCATGGCAcc                <  2:680829/140‑1 (MQ=255)                                                                                                                          ccAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAGGGAAAGGTAAATGACCGTGATGCACATGCATGGCACCAAATTCAg        <  2:670080/140‑1 (MQ=255)                                                                                                                          ccAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAGGGAAAGGTAAATGACCGTGATGCACATGCATGGCACCAAATTCAg        <  2:460329/140‑1 (MQ=255)                                                                                                                          ccAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAGGGAAAGGTAAATGACCGTGATGCACATGCATGGCACCAAATTCAg        >  2:1058279/1‑140 (MQ=255)                                                                                                                          ccAGCGGCGTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAGGGAAAGGTAAATGACCGTGATGCACATGCATGGCACCAAATTCAg        <  1:1058279/140‑1 (MQ=255)                                                                                                                              cggcgTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAGGGAAAGGTAAATGACCGTGATGCACATGCATGGCACCAAATTCAGcaca    <  1:314515/140‑1 (MQ=255)                                                                                                                               ggcgTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAGGGAAAGGTAAATGACCGTGATGCACATGCATGGCACCAAATTCAGCACAg   >  1:868386/1‑140 (MQ=255)                                                                                                                               ggcgTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAGGGAAAGGTAAATGACCGTGATGCACATGCATGGCACCAAATTCAGCACAg   >  2:955578/1‑140 (MQ=255)                                                                                                                                gcgTTTTGTCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAGGGAAAGGTAAATGACCGTGATGCACATGCATGGCACCAAATTCAGCACAGc  <  2:305239/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                     CGCGCGGTTATCACCAGCTCGGTCGGGTCGTAATTAACAATGCCGCGATGACAACGAACATCCAGCGTTTGCCCGGTGACAGGGCGACCTAAAAAGGCTTTGCTATTGCTGCCCTGAATCACCAGCGGCGTTTTATCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAGGGAAAGGTAAATGACCGTGATGCACATGCATGGCACCAAATTCAGCACAGC  >  NC_000913/3126326‑3126592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑