Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,152,295	T→C	T20T (ACT→ACC)	metC →	cystathionine beta‑lyase/L‑cysteine desulfhydrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,152,295	0	T	C	100.0%	332.6 / NA	91	T20T (ACT→ACC)	metC	cystathionine beta‑lyase/L‑cysteine desulfhydrase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/51);  total (40/51)

GCCAATTCTTCAGGATGCGCCCGCGAATATTCATGCTAGTTTAGACATCCAGACGTATAAAAACAGGAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACTCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGACAGTGTAGAAGCCAAAAAACACGCGACACGTAATCGCGCCAATGGAGAGTTGTTCTATGGACGGCGCGGAACGTTAACCCATTT  >  NC_000913/3152160‑3152429                                                                                                                                        |                                                                                                                                       gCCAATTCTTCAGGATGCGCCCGCGAATATTCATGCTAGTTTAGACATCCAGACGTATAAAAACAGGAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCg                                                                                                                                    <  1:445917/140‑1 (MQ=255) gCCAATTCTTCAGGATGCGCCCGCGAATATTCATGCTAGTTTAGACATCCAGACGTATAAAAACAGGAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCg                                                                                                                                    <  2:734398/140‑1 (MQ=255) gCCAATTCTTCAGGATGCGCCCGCGAATATTCATGCTAGTTTAGACATCCAGACGTATAAAAACAGGAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCg                                                                                                                                    <  1:784738/140‑1 (MQ=255)   cAATTCTTCAGGATGCGCCCGCGAATATTCATGCTAGTTTAGACATCCAGACGTATAAAAACAGGAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGgc                                                                                                                                  <  1:663182/140‑1 (MQ=255)     aTTCTTCAGGATGCGCCCGCGAATATTCATGCTAGTTTAGACATCCAGACGTATAAAAACAGGAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGgcgc                                                                                                                                >  1:759543/1‑140 (MQ=255)          tcAGGATGCGCCCGCGAATATTCATGCTAGTTTAGACATCCAGACGTATAAAAACAGGAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTaa                                                                                                                           >  2:601832/1‑140 (MQ=255)           cAGGATGCGCCCGCGAATATTCATGCTAGTTTAGACATCCAGACGTATAAAAACAGGAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTaaa                                                                                                                          <  2:1002821/140‑1 (MQ=255)           cAGGATGCGCCCGCGAATATTCATGCTAGTTTAGACATCCAGACGTATAAAAACAGGAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTaaa                                                                                                                          <  1:601832/140‑1 (MQ=255)           cAGGATGCGCCCGCGAATATTCATGCTAGTTTAGACATCCAGACGTATAAAAACAGGAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTaaa                                                                                                                          <  1:189121/140‑1 (MQ=255)             ggATGCGCCCGCGAATATTCATGCTAGTTTAGACATCCAGACGTATAAAAACAGGAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGcgcg                                                                                                                               >  2:219992/1‑133 (MQ=255)             ggATGCGCCCGCGAATATTCATGCTAGTTTAGACATCCAGACGTATAAAAACAGGAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGcgcg                                                                                                                               <  1:219992/133‑1 (MQ=255)             ggATGCGCCCGCGAATATTCATGCTAGTTTAGACATCCAGACGTATAAAAACAGGAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAACAAAAAATACACCCTCGGcgcg                                                                                                                               >  2:184063/1‑133 (MQ=255)             ggATGCGCCCGCGAATATTCATGCTAGTTTAGACATCCAGACGTATAAAAACAGGAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAACAAAAAATACACCCTCGGcgcg                                                                                                                               <  1:184063/133‑1 (MQ=255)              gATGCGCCCGCGAATATTCATGCTAGTTTAGACATCCAGACGTATAAAAACAGGAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAg                                                                                                                       >  1:142091/1‑140 (MQ=255)                tGCGCCCGCGAATATTCATGCTAGTTTAGACATCCAGACGCATAAAAACAGGAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCg                                                                                                                     >  1:660802/1‑140 (MQ=255)                 gcgcCCGCGAATATTCATGCTAGTTTAGACATCCAGACGTATAAAAACAGGAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCg                                                                                                                                    >  2:479420/1‑124 (MQ=255)                 gcgcCCGCGAATATTCATGCTAGTTTAGACATCCAGACGTATAAAAACAGGAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCg                                                                                                                                    <  1:479420/124‑1 (MQ=255)                   gcCCGCGAATATTCATGCTAGTTTAGACATCCAGACGTATAAAAACAGGAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCgg                                                                                                                                   >  2:355708/1‑123 (MQ=255)                   gcCCGCGAATATTCATGCTAGTTTAGACATCCAGACGTATAAAAACAGGAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCgg                                                                                                                                   <  1:355708/123‑1 (MQ=255)                   gcCCGCGAATATTCATGCTAGTTTAGACATCCAGACGTATAAAAACAGGAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGa                                                                                                                  >  1:252583/1‑140 (MQ=255)                        cgAATATTCATGCTAGTTTAGACATCCAGACGTATAAAAACAGGAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAg                                                                                                             >  1:354167/1‑140 (MQ=255)                               tCATGCTAGTTTAGACATCCAGACGTATAAAAACAGGAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGctt                                                                                                      <  1:738941/140‑1 (MQ=255)                               tCATGCTAGTTTAGACATCCAGACGTATAAAAACAGGAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGctt                                                                                                      <  1:594806/140‑1 (MQ=255)                               tCATGCTAGTTTAGACATCCAGACGTATAAAAACAGGAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGctt                                                                                                      <  2:142091/140‑1 (MQ=255)                                 aTGCTAGTTTAGACATCCAGACGTATAAAAACAGGAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGTCGCGGTAAATAGCGTGATTCAGCGCGcttct                                                                                                    >  2:557466/1‑140 (MQ=255)                                     tAGTTTAGACATCCAGACGTATAAAAACAGGAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGTCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGc                                                                                                <  1:557466/140‑1 (MQ=255)                                     tAGTTTAGACATCCAGACGTATAAAAACAGGAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGc                                                                                                <  1:684982/140‑1 (MQ=255)                                     tAGTTTAGACATCCAGACGTATAAAAACAGGAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGc                                                                                                >  2:565823/1‑140 (MQ=255)                                     tAGTTTAGACATCCAGACGTATAAAAACAGGAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGc                                                                                                <  1:1008566/140‑1 (MQ=255)                                     tAGTTTAGACATCCAGACGTATAAAAACAGGAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGc                                                                                                <  2:354167/140‑1 (MQ=255)                                        tttAGACATCCAGACGTATAAAAACAGGAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTgg                                                                                             >  1:350205/1‑140 (MQ=255)                                        tttAGACATCCAGACGTATAAAAACAGGAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTgg                                                                                             >  1:837645/1‑140 (MQ=255)                                             aCATCCAGACGTATAAAAACAGGAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGc                                                                                                >  2:788479/1‑132 (MQ=255)                                             aCATCCAGACGTATAAAAACAGGAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGc                                                                                                <  1:788479/132‑1 (MQ=255)                                               aTCCAGACGTATAAAAACAGGAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGa                                                                                      <  1:281726/140‑1 (MQ=255)                                               aTCCAGACGTATAAAAACAGGAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGa                                                                                      >  1:797746/1‑140 (MQ=255)                                                 ccAGACGTATAAAAACAGGAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGACa                                                                                    >  1:634383/1‑140 (MQ=255)                                                 ccAGACGTATAAAAACAGGAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGACa                                                                                    >  1:107792/1‑140 (MQ=255)                                                 ccAGACGTATAAAAACAGGAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGACa                                                                                    >  2:795926/1‑140 (MQ=255)                                                 ccAGACGTATAAAAACAGGAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGACa                                                                                    >  2:791553/1‑140 (MQ=255)                                                 ccAGACGTATAAAAACAGGAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGACa                                                                                    <  2:112196/140‑1 (MQ=255)                                                 ccAGACGTATAAAAACAGGAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGACa                                                                                    >  1:700376/1‑140 (MQ=255)                                                       gTATAAAAACAGGAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGACAGTGTAg                                                                              <  2:362437/140‑1 (MQ=255)                                                          taAAAACAGGAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATATCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGa                                                                                      >  1:1140931/1‑129 (MQ=255)                                                          taAAAACAGGAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATATCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGa                                                                                      <  2:1140931/129‑1 (MQ=255)                                                          taAAAACAGGAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGa                                                                                      <  1:932678/129‑1 (MQ=255)                                                          taAAAACAGGAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGa                                                                                      >  2:932678/1‑129 (MQ=255)                                                          taAAAACAGGAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGACAGTGTAGAAg                                                                           >  2:981524/1‑140 (MQ=255)                                                           aaaaaCAGGAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGACAGTGTAGAAGc                                                                          >  2:1122869/1‑140 (MQ=255)                                                               aCAGGAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGACAGTGTAGAAGCCaaa                                                                      >  2:587727/1‑140 (MQ=255)                                                                   gAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGACAGTGTAGAAGCCAAAAAac                                                                  >  1:1067950/1‑140 (MQ=255)                                                                   gAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGACAGTGTAGAAGCCAAAAAac                                                                  >  2:1102712/1‑140 (MQ=255)                                                                   gAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGACAGTGTAGAAGCCAAAAAac                                                                  >  1:1751/1‑140 (MQ=255)                                                                      tCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGACAGTGTAGAAGCCAAAAAACAcg                                                               <  2:952770/140‑1 (MQ=255)                                                                      tCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGACAGTGTAGAAGCCAAAAAACAcg                                                               <  2:991946/140‑1 (MQ=255)                                                                      tCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGACAGTGTAGAAGCCAAAAAACAcg                                                               <  2:660802/140‑1 (MQ=255)                                                                      tCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGACAGTGTAGAAGCCAAAAAACAcg                                                               <  1:1102712/140‑1 (MQ=255)                                                                      tCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGACAGTGTAGAAGCCAAAAAACAcg                                                               <  1:740936/140‑1 (MQ=255)                                                                        ccGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGACAGTGTAGAAGCCAAAAAACAcgcg                                                             <  1:1121596/140‑1 (MQ=255)                                                                         cGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGACAGTGTAGAAGCCAAAAAACACGCGa                                                            >  1:960622/1‑140 (MQ=255)                                                                           aCATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGACAGTGTAGAAGCCAAAAAACACGCGaca                                                          <  1:1122869/140‑1 (MQ=255)                                                                            cATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGACAGTGTAGAAGCCAAAAAACACGCGacac                                                         <  2:700376/140‑1 (MQ=255)                                                                                gCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGACAGTGTAGAAGCCAAAAAACACGCGACACGTaa                                                     >  1:1198764/1‑140 (MQ=255)                                                                                 cGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGACAGTGTAGAAGCCAAAAAACACGCGACACGTAAt                                                    <  2:837645/140‑1 (MQ=255)                                                                                     cAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGACAGTGTAGAAGCCAAAAAACACGCGACACGTAATcgcg                                                <  1:795926/140‑1 (MQ=255)                                                                                     cAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGACAGTGTAGAAGCCAAAAAACACGCGACACGTAATcgcg                                                >  2:101327/1‑140 (MQ=255)                                                                                      aaaaaGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGACAGTGTAGAAGCCAAAAAACACGCGACACGTAATCgcgc                                               <  2:532665/140‑1 (MQ=255)                                                                                        aaaGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGACAGTGTAGAAGCCAAAAAACACGCGACACGTAATCGCGCCa                                             <  2:252583/140‑1 (MQ=255)                                                                                        aaaGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGACAGTGTAGAAGCCAAAAAACACGCGACACGTAATCGCGCCa                                             <  1:565823/140‑1 (MQ=255)                                                                                             ttGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGACAGTGTAGAAGCCAAAAAACACGCGACACGTAATCGCGCCAATGga                                        <  1:981524/140‑1 (MQ=255)                                                                                             ttGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGACAGTGTAGAAGCCAAAAAACACGCGACACGTAATCGCGCCAATGga                                        <  2:1067950/140‑1 (MQ=255)                                                                                               gATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGACAGTGTAGAAGCCAAAAAACACGCGACACGTAATcg                                                  >  1:829442/1‑128 (MQ=255)                                                                                               gATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGACAGTGTAGAAGCCAAAAAACACGCGACACGTAATcg                                                  <  2:829442/128‑1 (MQ=255)                                                                                                aTACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGACAGTGTAGAAGCCAAAAAACACGCGACACGTAATCGCGCCAATGGagag                                     <  1:101327/140‑1 (MQ=255)                                                                                                  aCTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGACAGTGTAGAAGCCAAAAAACACGCGACACGTAATCGCGCCAATGGAGAgtt                                   <  2:960622/140‑1 (MQ=255)                                                                                                  aCTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGACAGTGTAGAAGCCAAAAAACACGCGACACGTAATCGCGCCAATGGAGAgtt                                   <  2:1155771/140‑1 (MQ=255)                                                                                                     cAACTGGTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGACAGTGTAGAAGCCAAAAAACACGCGACACGTAATCGCGCCAATGGAGAgttgtt                                <  2:797746/140‑1 (MQ=255)                                                                                                           gTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGACAGTGTAGAAGCCAAAAAACACGCGACACGTAATCGCGCCAATGGAGAgtt                                   <  2:617116/131‑1 (MQ=255)                                                                                                           gTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGACAGTGTAGAAGCCAAAAAACACGCGACACGTAATCGCGCCAATGGAGAgtt                                   >  1:617116/1‑131 (MQ=255)                                                                                                           gTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGACAGTGTAGAAGCCAAAAAACACGCGACACGTAATCGCGCCAATGGAGAgt                                    >  1:346292/1‑130 (MQ=255)                                                                                                           gTGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGACAGTGTAGAAGCCAAAAAACACGCGACACGTAATCGCGCCAATGGAGAgt                                    <  2:346292/130‑1 (MQ=255)                                                                                                            tGAATGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGACAGTGTAGAAGCCAAAAAACACGCGACACGTAATCGCGCCAATGGAGAGTTGTTCTATGGa                         <  2:634383/140‑1 (MQ=255)                                                                                                                tGCAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGACAGTGTAGAAGCCAAAAAACACGCGACACGTAATCGCGCCAATGGAGAGTTGTTCTATGGACGgc                     >  1:741768/1‑140 (MQ=255)                                                                                                                  cAGGACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGACAGTGTAGAAGCCAAAAAACACGCGACACGTAATCGCGCCAATGGAGAGTTGTTCTATGGACGgcgc                   >  1:832613/1‑140 (MQ=255)                                                                                                                     gACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGACAGTGTAGAAGCCAAAAAACACGCGACACGTAATCGCGCCAATGGAGAGTTGTTCTATGGACGGCACgg                 >  1:112066/1‑139 (MQ=255)                                                                                                                     gACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGACAGTGTAGAAGCCAAAAAACACGCGACACGTAATCGCGCCAATGGAGAGTTGTTCTATGGACGGCACgg                 <  2:112066/139‑1 (MQ=255)                                                                                                                     gACGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAACGCGCTTCTTCGCTGGTCTTTGACAGTGTAGAAGCCAAAAAACACGCGACACGTAATCGCGCCAATGGAGAGTTGTTCTATGGACGGCGCGGa                >  2:558788/1‑140 (MQ=255)                                                                                                                      aCGCAGCAAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGACAGTGTAGAAGCCAAAAAACACGCGACACGTAATCGCGCCAATGGAGAGTTGTTCTATGGACGGCGCGGaa               >  2:370589/1‑140 (MQ=255)                                                                                                                            caAAAAATACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGACAGTGTAGAAGCCAAAAAACACGCGACACGTAATCGCGCCAATGGAGAGTTGTTCTATGGACGGCGCGGAACGTTaa         <  2:107792/140‑1 (MQ=255)                                                                                                                                 aaTACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGACAGTGTAGAAGCCAAAAAACACGCGACACGTAATCGCGCCAATGGAGAGTTGTTCTATGGACGGCGCGGAACGTTAACCCAt    <  1:587727/140‑1 (MQ=255)                                                                                                                                   tACACCCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGACAGTGTAGAAGCCAAAAAACACGCGACACGTAATCGCGCCAATGGAGAGTTGTTCTATGGACGGCGCGGAACGTTAACCCAttt  <  2:832613/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       GCCAATTCTTCAGGATGCGCCCGCGAATATTCATGCTAGTTTAGACATCCAGACGTATAAAAACAGGAATCCCGACATGGCGGACAAAAAGCTTGATACTCAACTGGTGAATGCAGGACGCAGCAAAAAATACACTCTCGGCGCGGTAAATAGCGTGATTCAGCGCGCTTCTTCGCTGGTCTTTGACAGTGTAGAAGCCAAAAAACACGCGACACGTAATCGCGCCAATGGAGAGTTGTTCTATGGACGGCGCGGAACGTTAACCCATTT  >  NC_000913/3152160‑3152429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑