Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,179,729	T→C	V45A (GTC→GCC)	ygiA →	putative protein YgiA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,179,729	0	T	C	100.0%	248.5 / NA	69	V45A (GTC→GCC)	ygiA	putative protein YgiA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (42/27);  total (42/27)

ACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGTCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGTGA  >  NC_000913/3179592‑3179865                                                                                                                                          |                                                                                                                                         aCTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGccc                                                                                                                                        >  2:853754/1‑140 (MQ=255)     atgaCGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGCCCCGGa                                                                                                                                    >  1:107642/1‑140 (MQ=255)     atgaCGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGCCCCGGa                                                                                                                                    >  1:816461/1‑140 (MQ=255)     atgaCGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGCCCCGGa                                                                                                                                    >  2:745359/1‑140 (MQ=255)     atgaCGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGCCCCGGa                                                                                                                                    >  1:633604/1‑140 (MQ=255)                      tCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGCCCCGGAAGACAAAAATGAAACgg                                                                                                                   <  2:723848/140‑1 (MQ=255)                             cGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAAt                                                                                                            >  1:822748/1‑140 (MQ=255)                                 tGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCAt                                                                                                        <  2:514946/140‑1 (MQ=255)                                      gTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGcc                                                                                                   <  2:822748/140‑1 (MQ=255)                                         aGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACg                                                                                                >  1:700225/1‑140 (MQ=255)                                          ggCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGc                                                                                               >  1:23343/1‑140 (MQ=255)                                          ggCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGc                                                                                               >  2:105042/1‑140 (MQ=255)                                               cGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGt                                                                                          >  2:201148/1‑140 (MQ=255)                                                  aaaGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTcc                                                                                       <  2:107642/140‑1 (MQ=255)                                                     gATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCa                                                                                    >  1:279472/1‑140 (MQ=255)                                                          gggTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAAc                                                                               >  2:115204/1‑140 (MQ=255)                                                          gggTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAAc                                                                               >  2:465381/1‑140 (MQ=255)                                                          gggTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAAc                                                                               >  2:978438/1‑140 (MQ=255)                                                           ggTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACt                                                                              >  1:1127410/1‑140 (MQ=255)                                                               aTCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAg                                                                          <  2:1127410/140‑1 (MQ=255)                                                                tCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAgc                                                                         >  1:31183/1‑140 (MQ=255)                                                                tCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAgc                                                                         >  1:873778/1‑140 (MQ=255)                                                                  tGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAgcgc                                                                       >  1:4518/1‑140 (MQ=255)                                                                   gCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCa                                                                      <  2:31183/140‑1 (MQ=255)                                                                   gCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCa                                                                      <  2:722219/140‑1 (MQ=255)                                                                     cGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACg                                                                    <  1:105042/140‑1 (MQ=255)                                                                     cGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACg                                                                    <  1:1127783/140‑1 (MQ=255)                                                                     cGCATTCTTCCCCCTTCTCGCTTCAACTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACg                                                                    <  2:1091835/140‑1 (MQ=255)                                                                      gCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGc                                                                   <  2:700225/140‑1 (MQ=255)                                                                         ttcttcCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCAt                                                                >  1:970420/1‑140 (MQ=255)                                                                          tcttcCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATc                                                               >  1:632611/1‑140 (MQ=255)                                                                           cttcCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCt                                                              <  2:970420/140‑1 (MQ=255)                                                                                    tctcGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGt                                                     >  2:360361/1‑140 (MQ=255)                                                                                    tctcGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAAACCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACg                                                                    >  1:576574/1‑125 (MQ=255)                                                                                    tctcGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAAACCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACg                                                                    <  2:576574/125‑1 (MQ=255)                                                                                             aaTTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTcgc                                            <  1:853754/140‑1 (MQ=255)                                                                                                   gACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGc                                      <  2:873778/140‑1 (MQ=255)                                                                                                     ccAGCCATCCTCTATTCTGATGGGTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCa                                    <  1:745359/140‑1 (MQ=255)                                                                                                        gCCATCCTCTATTCTGATGGGTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTg                                 >  2:395476/1‑140 (MQ=255)                                                                                                                   ttCTGATGGGTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATgctg                      >  2:453419/1‑140 (MQ=255)                                                                                                                   ttCTGATGGGTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATgctg                      >  2:223585/1‑140 (MQ=255)                                                                                                                   ttCTGATGGGTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATgctg                      >  1:641823/1‑140 (MQ=255)                                                                                                                      tGATGGGTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATgctggct                   >  1:252701/1‑140 (MQ=255)                                                                                                                       ggtGGGTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATgctggctg                  >  2:187057/3‑140 (MQ=255)                                                                                                                       gATGGGTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATgctggctg                  <  2:4518/140‑1 (MQ=255)                                                                                                                       gATGGGTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATgctggctg                  >  1:1014300/1‑140 (MQ=255)                                                                                                                       gATGGGTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATgctggctg                  >  1:90321/1‑140 (MQ=255)                                                                                                                        aTGGGTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATgctggctgg                 >  2:1107838/1‑140 (MQ=255)                                                                                                                        aTGGGTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATgctggctgg                 >  2:24530/1‑140 (MQ=255)                                                                                                                        aTGGGTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATgctggctgg                 >  1:997537/1‑140 (MQ=255)                                                                                                                        aTGGGTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATgctggctgg                 >  1:927601/1‑140 (MQ=255)                                                                                                                        aTGGGTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATgctggc                    <  1:442261/137‑1 (MQ=255)                                                                                                                        aTGGGTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATgctggc                    >  2:442261/1‑137 (MQ=255)                                                                                                                         tGGGTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATgctggctggc                <  1:978438/140‑1 (MQ=255)                                                                                                                          gggTATTTACCACTGGCCCCGGAAGTCAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATgctggc                    <  2:753373/135‑1 (MQ=255)                                                                                                                          gggTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATgctggc                    >  1:753373/1‑135 (MQ=255)                                                                                                                           ggTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATgctggctggctg              >  1:24017/1‑140 (MQ=255)                                                                                                                           ggTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATgctggctggctg              >  2:1076009/1‑140 (MQ=255)                                                                                                                           ggTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATgctggc                    <  2:283899/134‑1 (MQ=255)                                                                                                                           ggTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATgctggc                    >  1:283899/1‑134 (MQ=255)                                                                                                                            gTATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCtgt             <  2:252701/140‑1 (MQ=255)                                                                                                                             tATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATgctggctggc                <  2:33119/136‑1 (MQ=255)                                                                                                                             tATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATgctggctggc                >  1:33119/1‑136 (MQ=255)                                                                                                                             tATTTACCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCtgtg            >  1:1213924/1‑140 (MQ=255)                                                                                                                                  aCCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAag       >  1:281542/1‑140 (MQ=255)                                                                                                                                  aCCACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAag       <  1:465381/140‑1 (MQ=255)                                                                                                                                   ccACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCT‑GCTGTGAAAagag     <  1:187057/140‑1 (MQ=255)                                                                                                                                    cACTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAagag     <  1:1076009/140‑1 (MQ=255)                                                                                                                                      cTGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGtg   >  1:186932/1‑140 (MQ=255)                                                                                                                                       tGGCCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGtga  <  1:259378/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         ACTGATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGTCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGTGA  >  NC_000913/3179592‑3179865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑