Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	220,231	G→A	T233M (ACG→ATG)	metQ ←	L‑methionine/D‑methionine ABC transporter membrane anchored binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	220,231	0	G	A	100.0%	333.5 / NA	92	T233M (ACG→ATG)	metQ	L‑methionine/D‑methionine ABC transporter membrane anchored binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (40/52);  total (40/52)

TGTGGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGCGTCGTCCAGAGAACGCGG  >  NC_000913/220094‑220367                                                                                                                                          |                                                                                                                                         tgtgGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATc                                                                                                                                        >  2:552217/1‑140 (MQ=255)   tgGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCAc                                                                                                                                      >  2:523833/1‑140 (MQ=255)   tgGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCAc                                                                                                                                      >  2:1207993/1‑140 (MQ=255)   tgGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCAc                                                                                                                                      >  2:118952/1‑140 (MQ=255)   tgGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCAc                                                                                                                                      >  2:212156/1‑140 (MQ=255)     gAAACAGCCTAAAAATTCCCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGa                                                                                                                                    <  1:523833/140‑1 (MQ=255)     gAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGa                                                                                                                                    <  2:277952/140‑1 (MQ=255)      aaaCAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGAt                                                                                                                                   <  1:1207993/140‑1 (MQ=255)      aaaCAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGAt                                                                                                                                   <  1:212156/140‑1 (MQ=255)       aaCAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATc                                                                                                                                  <  1:118952/140‑1 (MQ=255)       aaCAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATc                                                                                                                                  <  1:1174176/140‑1 (MQ=255)            ccTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGtt                                                                                                                             >  1:899100/1‑140 (MQ=255)                aaaaTTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTtacg                                                                                                                         <  2:1212156/140‑1 (MQ=255)                      cccAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACggg                                                                                                                   <  2:1071184/139‑1 (MQ=255)                      aCCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACggg                                                                                                                   <  2:899100/140‑1 (MQ=255)                      aCCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACggg                                                                                                                   >  2:1214139/1‑140 (MQ=255)                      aCCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACggg                                                                                                                   <  2:1229197/140‑1 (MQ=255)                      aCCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACggg                                                                                                                   <  2:678766/140‑1 (MQ=255)                      aCCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACggg                                                                                                                   >  1:685835/1‑140 (MQ=255)                      aCCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACggg                                                                                                                   <  1:552217/140‑1 (MQ=255)                      aCCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACggg                                                                                                                   <  1:543346/140‑1 (MQ=255)                      aCCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACggg                                                                                                                   <  1:1208178/140‑1 (MQ=255)                         aGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGAc                                                                                                                <  1:445006/140‑1 (MQ=255)                          gCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGAct                                                                                                               >  2:1230890/1‑140 (MQ=255)                              ttAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCttt                                                                                                           <  1:188864/140‑1 (MQ=255)                                    gCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTc                                                                                                     <  2:942602/140‑1 (MQ=255)                                     cTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTca                                                                                                    >  2:119498/1‑140 (MQ=255)                                     cTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTca                                                                                                    >  1:1002520/1‑140 (MQ=255)                                     cTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTca                                                                                                    >  2:201552/1‑140 (MQ=255)                                     cTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTca                                                                                                    >  1:308863/1‑140 (MQ=255)                                     cTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTca                                                                                                    >  2:1044021/1‑140 (MQ=255)                                      tCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTcaa                                                                                                   >  2:1226828/1‑140 (MQ=255)                                      tCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTcaa                                                                                                   <  1:824562/140‑1 (MQ=255)                                        cgccgTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTGCGTACGGGGACTCTTTATCTTc                                                                                                     <  1:256477/136‑1 (MQ=255)                                        cgccgTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTGCGTACGGGGACTCTTTATCTTc                                                                                                     >  2:256477/1‑136 (MQ=255)                                          ccgTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACaaa                                                                                               >  1:682949/1‑140 (MQ=255)                                          ccgTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACaaa                                                                                               >  1:839671/1‑140 (MQ=255)                                            gTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGa                                                                                             <  1:581151/140‑1 (MQ=255)                                              tAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATa                                                                                           <  1:201552/140‑1 (MQ=255)                                                 acacTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCg                                                                                        <  1:1214139/140‑1 (MQ=255)                                                     tttgtttgCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCtt                                                                                    >  1:1212399/1‑140 (MQ=255)                                                     tttgtttgCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCtt                                                                                    <  1:119498/140‑1 (MQ=255)                                                     tttgtttgCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCtt                                                                                    <  1:660001/140‑1 (MQ=255)                                                          ttgCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGcc                                                                               <  2:413649/140‑1 (MQ=255)                                                             ctgctTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGa                                                                            >  2:165586/1‑140 (MQ=255)                                                              tgctTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAg                                                                           <  2:839393/140‑1 (MQ=255)                                                              tgctTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAg                                                                           <  2:538051/140‑1 (MQ=255)                                                              tgctTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAg                                                                           <  1:1230890/140‑1 (MQ=255)                                                               gctTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGt                                                                          >  1:80497/1‑140 (MQ=255)                                                               gctTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGt                                                                          >  1:35248/1‑140 (MQ=255)                                                               gctTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGt                                                                          <  1:499400/140‑1 (MQ=255)                                                                  tCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAg                                                                       <  1:889155/140‑1 (MQ=255)                                                                  tCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAg                                                                       >  2:889155/1‑140 (MQ=255)                                                                   cGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAgg                                                                      >  1:561234/1‑140 (MQ=255)                                                                   cGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAgg                                                                      <  2:80497/140‑1 (MQ=255)                                                                           tCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATctg                                                              <  2:214871/140‑1 (MQ=255)                                                                            cGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATctgg                                                             <  2:682949/140‑1 (MQ=255)                                                                            cGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATctgg                                                             >  2:648408/1‑140 (MQ=255)                                                                            cGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATctgg                                                             >  2:576496/1‑140 (MQ=255)                                                                            cGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATctgg                                                             >  2:930748/1‑140 (MQ=255)                                                                               cAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATctggctg                                                          >  2:994413/1‑140 (MQ=255)                                                                                 gACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCtggctggc                                                        <  1:1044021/140‑1 (MQ=255)                                                                                  aCTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCtggctggc                                                        <  1:261714/139‑1 (MQ=255)                                                                                  aCTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCtggctggc                                                        >  2:261714/1‑139 (MQ=255)                                                                                   cTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCAt                                                      <  1:994413/140‑1 (MQ=255)                                                                                   cTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCAt                                                      <  2:561234/140‑1 (MQ=255)                                                                                     gATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAg                                                    <  1:881602/140‑1 (MQ=255)                                                                                      aTAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAgg                                                   <  2:1044634/140‑1 (MQ=255)                                                                                      aTAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAgg                                                   <  2:1002520/140‑1 (MQ=255)                                                                                      aTAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAgg                                                   <  2:35248/140‑1 (MQ=255)                                                                                       tAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAggt                                                  >  1:169654/1‑140 (MQ=255)                                                                                        aaGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAggtg                                                 <  2:734819/140‑1 (MQ=255)                                                                                        aaGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAggtg                                                 <  1:1226828/140‑1 (MQ=255)                                                                                          gCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAggtggt                                               <  2:1212399/140‑1 (MQ=255)                                                                                            cTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTAt                                             >  2:785201/1‑140 (MQ=255)                                                                                                aCGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGAt                                         <  1:930748/140‑1 (MQ=255)                                                                                                aCGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGAt                                         <  2:501922/140‑1 (MQ=255)                                                                                                aCGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGAt                                         <  2:308863/140‑1 (MQ=255)                                                                                                aCGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGAt                                         >  2:174623/1‑140 (MQ=255)                                                                                                aCGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGAt                                         <  1:648408/140‑1 (MQ=255)                                                                                                     tttCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTg                                    >  1:103006/1‑140 (MQ=255)                                                                                                         ttcACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCag                                >  2:1186724/1‑140 (MQ=255)                                                                                                           cACGTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCagag                              <  1:165586/140‑1 (MQ=255)                                                                                                              gTTCTCGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGa                           >  2:1033828/1‑140 (MQ=255)                                                                                                                   cGGCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTgc                      >  1:795260/1‑140 (MQ=255)                                                                                                                     gCGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTgcgc                    <  1:785201/140‑1 (MQ=255)                                                                                                                      cGTCTTTGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGcgcg                   <  1:1186724/140‑1 (MQ=255)                                                                                                                          tttGTTATCTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGcgtcgt               >  1:389111/1‑140 (MQ=255)                                                                                                                                  cTTCACGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGCGTCGTCCAGAGaa       >  1:650985/1‑140 (MQ=255)                                                                                                                                      aCGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGCGTCGTCCAGAGAAcgcg   >  1:507768/1‑140 (MQ=255)                                                                                                                                      aCGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGCGTCGTCCAGAGAAcgc    <  1:506232/139‑1 (MQ=255)                                                                                                                                      aCGCATCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGCGTCGTCCAGAGAAcgc    >  2:506232/1‑139 (MQ=255)                                                                                                                                       cgcaTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGCGTCGTCCAGAGAACGCgg  <  1:1032282/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         TGTGGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGCGTCGTCCAGAGAACGCGG  >  NC_000913/220094‑220367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑