Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,519,289	T→C	intergenic (‑225/+176)	aroK ← / ← hofQ	shikimate kinase 1/DNA utilization protein HofQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,519,289	0	T	C	100.0%	345.0 / NA	95	intergenic (‑225/+176)	aroK/hofQ	shikimate kinase 1/DNA utilization protein HofQ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (48/47);  total (48/47)

TGCGAGAGTCAGACTGAAAATTATCTCAATACTCCAGCGGGTTTGGCAACTGAATAAATCACCCATCCCTCTGCATATCTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGTAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGCAGCGAAGAGCACCAACTCTCAAATCGGTACTCCTTGTATGCTAAATACCTGCGCGCGTCAAATAGAT  >  NC_000913/3519155‑3519422                                                                                                                                       |                                                                                                                                      tGCGAGAGTCAGACTGAAAATTATCTCAATACTCCAGCGGGTTTGGCAACTGAATAAATCACCCATCCCTCTGCATATCTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGAt                                                                                                                                  >  1:882190/1‑140 (MQ=255) tGCGAGAGTCAGACTGAAAATTATCTCAATACTCCAGCGGGTTTGGCAACTGAATAAATCACCCATCCCTCTGCATATCTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGAt                                                                                                                                  >  1:1110555/1‑140 (MQ=255) tGCGAGAGTCAGACTGAAAATTATCTCAATACTCCAGCGGGTTTGGCAACTGAATAAATCACCCATCCCTCTGCATATCTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGAt                                                                                                                                  >  1:76927/1‑140 (MQ=255) tGCGAGAGTCAGACTGAAAATTATCTCAATACTCCAGCGGGTTTGGCAACTGAATAAATCACCCATCCCTCTGCATATCTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGAt                                                                                                                                  >  1:330754/1‑140 (MQ=255) tGCGAGAGTCAGACTGAAAATTATCTCAATACTCCAGCGGGTTTGGCAACTGAATAAATCACCCATCCCTCTGCATATCTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGAt                                                                                                                                  <  1:269263/140‑1 (MQ=255)  gCGAGAGTCAGACTGAAAATTATCTCAATACTCCAGCGGGTTTGGCAACTGAATAAATCACCCATCCCTCTGCATATCTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGAtt                                                                                                                                 >  2:123113/1‑140 (MQ=255)     agagTCAGACTGAAAATTATCTCAATACTCCAGCGGGTTTGGCAACTGAATAAATCACCCATCCCTCTGCATATCTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGc                                                                                                                              <  2:396221/140‑1 (MQ=255)                           cAATACTCCAGCGGGTTTGGCAACTGAATAAATCACCCATCCCTCTGCATATCTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATgcc                                                                                                        >  2:687386/1‑140 (MQ=255)                                   cAGCGGGTTTGGCAACTGAATAAATCACCCATCCCTCTGCATATCTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAAt                                                                                                >  2:386102/1‑140 (MQ=255)                                   cAGCGGGTTTGGCAACTGAATAAATCACCCATCCCTCTGCATATCTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAAt                                                                                                >  1:700678/1‑140 (MQ=255)                                   cAGCGGGTTTGGCAACTGAATAAATCACCCATCCCTCTGCATATCTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAAt                                                                                                >  1:628301/1‑140 (MQ=255)                                    aGCGGGTTTGGCAACTGAATAAATCACCCATCCCTCTGCATATCTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATg                                                                                               >  1:246958/1‑140 (MQ=255)                                      cGGGTTTGGCAACTGAATAAATCACCCATCCCTCTGCATATCTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGAt                                                                                             <  2:991018/140‑1 (MQ=255)                                      cGGGTTTGGCAACTGAATAAATCACCCATCCCTCTGCATATCTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGAt                                                                                             >  1:191621/1‑140 (MQ=255)                                        ggTTTGGCAACTGAATAAATCACCCATCCCTCTGCATATCTGGTCGCTGTAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATaa                                                                                           >  1:1132671/1‑140 (MQ=255)                                        ggTTTGGCAACTGAATAAATCACCCATCCCTCTGCATATCTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAAt                                                                                                <  2:1049307/135‑1 (MQ=255)                                        ggTTTGGCAACTGAATAAATCACCCATCCCTCTGCATATCTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAAt                                                                                                >  1:1049307/1‑135 (MQ=255)                                        ggTTTGGCAACTGAATAAATCACCCATCCCTCTGCATATCTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATaa                                                                                           >  2:257280/1‑140 (MQ=255)                                        ggTTTGGCAACTGAATAAATCACCCATCCCTCTGCATATCTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATaa                                                                                           >  2:464136/1‑140 (MQ=255)                                          tttGGCAACTGAATAAATCACCCATCCCTCTGCATATCTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAat                                                                                         <  2:525006/140‑1 (MQ=255)                                           ttGGCAACTGAATAAATCACCCATCCCTCTGCATATCTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAatc                                                                                        >  1:1015040/1‑140 (MQ=255)                                            tGGCAACTGAATAAATCACCCATCCCTCTGCATATCTGGTCGCTGTAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAatca                                                                                       <  2:1132671/140‑1 (MQ=255)                                            tGGCAACTGAATAAATCACCCATCCCTCTGCATATCTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAatca                                                                                       >  2:829513/1‑140 (MQ=255)                                             ggCAACTGAATAAATCACCCATCCCTCTGCATATCTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAatcat                                                                                      >  2:903828/1‑140 (MQ=255)                                             ggCAACTGAATAAATCACCCATCCCTCTGCATATCTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAatcat                                                                                      <  2:330754/140‑1 (MQ=255)                                             ggCAACTGAATAAATCACCCATCCCTCTGCATATCTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAatcat                                                                                      <  1:386102/140‑1 (MQ=255)                                               cAACTGAATAAATCACCCATCCCTCTGCATATCTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAatcatca                                                                                    <  2:882190/140‑1 (MQ=255)                                               cAACTGAATAAATCACCCATCCCTCTGCATATCTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAatcatca                                                                                    <  2:76927/140‑1 (MQ=255)                                                   tGAATAAATCACCCATCCCTCTGCATATCTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATcatcatcat                                                                                <  1:687386/140‑1 (MQ=255)                                                     aaTAAATCACCCATCCCTCTGCATATCTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAatcatca                                                                                    >  2:189453/1‑134 (MQ=255)                                                     aaTAAATCACCCATCCCTCTGCATATCTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAatcatca                                                                                    <  1:189453/134‑1 (MQ=255)                                                     aaTAAATCACCCATCCCTCTGCATATCTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATgg                                                                              >  2:60089/1‑140 (MQ=255)                                                          aTCACCCATCCCTCTGCATATCTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACgc                                                                         <  2:628301/140‑1 (MQ=255)                                                            cACCCATCCCTCTGCATATCTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATcatcatcat                                                                                >  2:518662/1‑131 (MQ=255)                                                            cACCCATCCCTCTGCATATCTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATcatcatcat                                                                                <  1:518662/131‑1 (MQ=255)                                                            cACCCATCCCTCTGCATATCTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACgcag                                                                       <  2:1110555/140‑1 (MQ=255)                                                              cccATCCCTCTGCATATCTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACgcaggc                                                                     >  2:539332/1‑140 (MQ=255)                                                               ccATCCCTCTGCATATCTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGTTTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACgcaggca                                                                    >  2:68046/1‑140 (MQ=255)                                                               ccATCCCTCTGCATATCTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACgcaggca                                                                    >  1:211734/1‑140 (MQ=255)                                                               ccATCCCTCTGCATATCTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACgcaggca                                                                    >  1:1026358/1‑140 (MQ=255)                                                               ccATCCCTCTGCATATCTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACgcaggca                                                                    >  1:1082990/1‑140 (MQ=255)                                                                   cccTCTGCATATCTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGCAGCGa                                                                >  2:978322/1‑140 (MQ=255)                                                                   cccTCTGCATATCTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGCAGCGa                                                                <  2:246958/140‑1 (MQ=255)                                                                    ccTCTGCATATCTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGCAGCGaa                                                               >  1:1175288/1‑140 (MQ=255)                                                                       ctGCATATCTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGCAGCGAagag                                                            >  1:601386/1‑140 (MQ=255)                                                                        tGCATATCTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGCAGCGAAGAGc                                                           >  2:630691/1‑140 (MQ=255)                                                                           atatCTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGCAGCGAAGAGCAcc                                                        <  2:508247/140‑1 (MQ=255)                                                                           atatCTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGCAGCGAAGAGCAcc                                                        <  1:261127/140‑1 (MQ=255)                                                                             atCTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGCAGCGAAGAGCACCaa                                                      >  2:782331/1‑140 (MQ=255)                                                                             atCTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGCAGCGAAGAGCACCaa                                                      >  1:1110389/1‑140 (MQ=255)                                                                             atCTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGCAGCGAAGAGCACCaa                                                      >  2:337762/1‑140 (MQ=255)                                                                             atCTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGCAGCGAAGAGCACCaa                                                      >  2:327214/1‑140 (MQ=255)                                                                              tCTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGCAGCGAAGAGCACCAAc                                                     >  2:367993/1‑140 (MQ=255)                                                                               cTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGTAGCGAAGAGCACCAAct                                                    >  2:30716/1‑140 (MQ=255)                                                                               cTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGCAGCGAAGAGCACCAAct                                                    >  1:310335/1‑140 (MQ=255)                                                                               cTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGCAGCGAAGAGCACCAAct                                                    >  1:639056/1‑140 (MQ=255)                                                                                 ggTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGCAGCGAAGAGCACCAActct                                                  <  1:367993/140‑1 (MQ=255)                                                                                 ggTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGCAGCGAAGAGCACCAActct                                                  <  2:1026358/140‑1 (MQ=255)                                                                                 ggTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGCAGCGAAGAGCACCAActct                                                  >  1:1106054/1‑140 (MQ=255)                                                                                  gTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGCAGCGAAGAGCACCAAc                                                     <  1:237466/136‑1 (MQ=255)                                                                                  gTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGCAGCGAAGAGCACCAAc                                                     >  2:237466/1‑136 (MQ=255)                                                                                  gTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGCAGCGAAGAGCACCAACtctc                                                 >  2:78824/1‑140 (MQ=255)                                                                                   tCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGCAGCGAAGAGCACCAACTCTCa                                                <  1:1126753/140‑1 (MQ=255)                                                                                    cGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGCAGCGAAGAGCACCAACTCTCaa                                               >  1:1148626/1‑140 (MQ=255)                                                                                     gCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGCAGCGAAGAGCACCAACTCTCaaa                                              >  2:286157/1‑140 (MQ=255)                                                                                      cTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGCAGCGAAGAGCACCAACTCTCAAAt                                             <  2:1220614/140‑1 (MQ=255)                                                                                         cAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGCAGCGAAGAGCACCAACTCTCAAATCgg                                          <  2:191621/140‑1 (MQ=255)                                                                                            gcgcgcTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGCAGCGAAGAGCACCAACTCTCAAATCGGTAc                                       <  2:1015040/140‑1 (MQ=255)                                                                                            gcgcgcTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGCAGCGAAGAGCACCAACTCTCAAATCGGTAc                                       <  2:601386/140‑1 (MQ=255)                                                                                            gcgcgcTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGCAGCGAAGAGCACCAACTCTCAAATCGGTAc                                       <  1:782331/140‑1 (MQ=255)                                                                                            gcgcgcTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGCAGCGAAGAGAACCAACTCTCAAATCGGTAc                                       <  2:670218/140‑1 (MQ=255)                                                                                             cgcgcTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGTAGCGAAGAGCACCAACTCTCAAATCGGTACt                                      <  1:30716/140‑1 (MQ=255)                                                                                                 cTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGCAGCGAAGAGCACCAACTCTCAAATCGGTACTCCtt                                  >  2:444768/1‑140 (MQ=255)                                                                                                 cTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGCAGCGAAGAGCACCAACTCTCAAATCGGTACTCCtt                                  <  1:60089/140‑1 (MQ=255)                                                                                                 cTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGCAGCGAAGAGCACCAACTCTCAAATCGGTACTCCtt                                  <  1:539332/140‑1 (MQ=255)                                                                                                 cTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGCAGCGAAGAGCACCAACTCTCAAATCGGTACTCCtt                                  <  2:700678/140‑1 (MQ=255)                                                                                                   gCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGCAGCGAAGAGCACCAACTCTCAAATCGGTACTCCTTGt                                <  1:327214/140‑1 (MQ=255)                                                                                                    ccTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGCAGCGAAGAGCACCAACTCTCAAATCGGTACTCCTTGTa                               <  1:829513/140‑1 (MQ=255)                                                                                                    ccTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGCAGCGAAGAGCACCAACTCTCAAATCGGTACTCCTTGTa                               <  1:978322/140‑1 (MQ=255)                                                                                                    ccTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGCAGCGAAGAGCACCAACTCTCAAATCGGTACTCCTTGTa                               <  2:310335/140‑1 (MQ=255)                                                                                                    ccTTGCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGCAGCGAAGAGCACCAACTCTCAAATCGGTACTCCTTGTa                               <  1:444768/140‑1 (MQ=255)                                                                                                        gCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATCAATCATCATCATGGAACGCAGGCAGCGAAGAGCACCAACTCTCAAATCGGTACTCCTTGTATGCt                           <  2:1082990/140‑1 (MQ=255)                                                                                                        gCTACCACCGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGCAGCGAAGAGCACCAACTCTCAAATCGGTACTCCTTGTATGCt                           <  1:257280/140‑1 (MQ=255)                                                                                                              accGCTCTGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGCAGCGAAGAGCACCAACTCTCAAATCGGTACTCCTTGTATGCTAAATAc                     >  1:209018/1‑140 (MQ=255)                                                                                                                  ctctGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACACAGGCAGCGAAGAGCACCAACTCTCAAATCGGTACTCCTTGTATGCTAAATACCTgc                 >  1:214261/1‑140 (MQ=255)                                                                                                                   tctGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGCAGCGAAGAGCACCAACTCTCAAATCGGTACTCCTTGTa                               >  2:782158/1‑125 (MQ=255)                                                                                                                   tctGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGCAGCGAAGAGCACCAACTCTCAAATCGGTACTCCTTGTa                               <  1:782158/125‑1 (MQ=255)                                                                                                                   tctGGCGATAAATCACCGAGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGCAGCGAAGAGCACCAACTCTCAAATCGGTACTCCTTGTATGCTAAATACCTgcg                >  1:932850/1‑140 (MQ=255)                                                                                                                   tctGGCGATAAATCACCGAGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGCAGCGAAGAGCACCAACTCTCAAATCGGTACTCCTTGTATGCTAAATACCTgcg                <  2:932850/140‑1 (MQ=255)                                                                                                                    ctGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGCAGCGAAGAGCACCAACTCTCAAATCGGTACTCCTTGTATGCTAAATACCTgcgc               <  2:1148626/140‑1 (MQ=255)                                                                                                                     tGGCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGCAGCGAAGAGCACCAACTCTCAAATCGGTACTCCTTGTATGCTAAATACCTgcgcg              <  2:1110389/140‑1 (MQ=255)                                                                                                                       gCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGCAGCGAAGAGCACCAACTCTCAAATCGGTACTCCTTGTATGCTAAATACCTGcgcgcg            <  2:803606/140‑1 (MQ=255)                                                                                                                       gCGATAAATCACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGCAGCGAAGAGCACCAACTCTCAAATCGGTACTCCTTGTATGCTAAATACCTGcgcgcg            <  2:937645/140‑1 (MQ=255)                                                                                                                                cACCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGCAGCGAAGAGCACCAACTCTCAAATCGGTACTCCTTGT‑TGCTAAATACCTGCGCGCGTCAAATAGAt  <  1:1020473/140‑1 (MQ=255)                                                                                                                                 aCCGGGCAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGCAGCGAAGAGCACCAACTCTCAAATCGGTACTCCTTGTATGCTAAATACCTGCGCGCGTCAAATAGAt  <  1:903828/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                      TGCGAGAGTCAGACTGAAAATTATCTCAATACTCCAGCGGGTTTGGCAACTGAATAAATCACCCATCCCTCTGCATATCTGGTCGCTGCAAGCGCGCTGCCTTGCTACCACCGCTCTGGCGATAAATCACCGGGTAAGATTAGCGTAAAAAAGACAGCAAAATGCCGCCTGAATGATAAATCATCATCATGGAACGCAGGCAGCGAAGAGCACCAACTCTCAAATCGGTACTCCTTGTATGCTAAATACCTGCGCGCGTCAAATAGAT  >  NC_000913/3519155‑3519422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑