Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,520,253	G→A	R151C (CGC→TGC)	hofQ ←	DNA utilization protein HofQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,520,253	0	G	A	100.0%	234.3 / NA	66	R151C (CGC→TGC)	hofQ	DNA utilization protein HofQ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/30);  total (36/30)

CGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCATTTGCGCTACCCACTGTTCAAGCGCGCTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCGTTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTC  >  NC_000913/3520117‑3520385                                                                                                                                         |                                                                                                                                     cGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCATTTGCGCTACCCACTGTTCAAGCGCGCTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCAttt                                                                                                                                   >  1:1101202/1‑140 (MQ=255) cGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCATTTGCGCTACCCACTGTTCAAGCGCGCTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCAttt                                                                                                                                   >  2:1053925/1‑140 (MQ=255) cGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCATTTGCGCTACCCACTGTTCAAGCGCGCTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCAttt                                                                                                                                   >  1:180194/1‑140 (MQ=255)  gCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCATTTGCGCTACCCACTGTTCAAGCGCGCTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCATTTa                                                                                                                                  <  2:928770/140‑1 (MQ=255)  gCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCATTTGCGCTACCCACTGTTCAAGCGCGCTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCATTTa                                                                                                                                  >  2:195802/1‑140 (MQ=255)      aCTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCATTTGCGCTACCCACTGTTCAAGCGCGCTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCATTTATCGa                                                                                                                              <  1:1053925/140‑1 (MQ=255)        ttttttCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCATTTGCGCTACCCACTGTTCAAGCGCGCTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCATTTATCGACg                                                                                                                            <  2:1213379/140‑1 (MQ=255)          ttttCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCATTTGCGCTACCCACTGTTCAAGCGCGCTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCATTTATCGa                                                                                                                              <  2:197867/136‑1 (MQ=255)          ttttCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCATTTGCGCTACCCACTGTTCAAGCGCGCTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCATTTATCGa                                                                                                                              >  1:197867/1‑136 (MQ=255)                  aaTGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCATTTGCGCTACCCACTGTTCAAGCGCGCTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCATTTATCGACGGTCATACTcc                                                                                                                  <  1:943225/140‑1 (MQ=255)                       tGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCATTTGCGCTACCCACTGTTCAAGCGCGCTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCATTTATCGACGGTCATACTCCCTTTg                                                                                                             <  2:472423/140‑1 (MQ=255)                       tGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCATTTGCGCTACCCACTGTTCAAGCGCGCTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCATTTATCGACGGTCATACTCCCTTTg                                                                                                             <  1:802711/140‑1 (MQ=255)                          cAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCATTTGCGCTACCCACTGTTCAAGCGCGCTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCATTTATCGACGGTCATACTCCCTTTGGCa                                                                                                          <  1:728110/140‑1 (MQ=255)                          cAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCATTTGCGCTACCCACTGTTCAAGCGCGCTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCATTTATCGACGGTCATACTCCCTTTGGCa                                                                                                          <  1:351406/140‑1 (MQ=255)                                gcgcCGACAGCTCAACCTGCCCGACCGGCAGATCCATTTGCGCTACCCACTGTTCAAGCGCGCTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCATTTATCGACGGTCATACTCCCTTTGGCACTCAGt                                                                                                    >  2:31478/1‑140 (MQ=255)                                gcgcCGACAGCTCAACCTGCCCGACCGGCAGATCCATTTGCGCTACCCACTGTTCAAGCGCGCTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCATTTATCGACGGTCATACTCCCTTTGGCACTCAGt                                                                                                    <  1:334765/140‑1 (MQ=255)                                 cgcCGACAGCTCAACCTGCCCGACCGGCAGATCCATTTGCGCTACCCACTGTTCAAGCGCGCTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCATTTATCGACGGTCATACTCCCTTTGGCACTCAGTa                                                                                                   >  2:436451/1‑140 (MQ=255)                                 cgcCGACAGCTCAACCTGCCCGACCGGCAGATCCATTTGCGCTACCCACTGTTCAAGCGCGCTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCATTTATCGACGGTCATACTCCCTTTGGCACTCAGTa                                                                                                   >  1:705881/1‑140 (MQ=255)                                 cgcCGACAGCTCAACCTGCCCGACCGGCAGATCCATTTGCGCTACCCACTGTTCAAGCGCGCTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCATTTATCGACGGTCATACTCCCTTTGGCACTCAGTa                                                                                                   >  1:613380/1‑140 (MQ=255)                                         gCTCAACCTGCCCGACCGGCAGATCCATTTGCGCTACCCACTGTTCAAGCGCGCTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCATTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTcc                                                                                           >  1:114100/1‑140 (MQ=255)                                          cTCAACCTGCCCGACCGGCAGATCCATTTGCGCTACCCACTGTTCAAGCGCGCTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCATTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTccc                                                                                          >  1:432663/1‑140 (MQ=255)                                                 tGCCCGACCGGCAGATCCATTTGCGCTACCCACTGTTCAAGCGCGCTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCATTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCttt                                                                                   >  1:183414/1‑140 (MQ=255)                                                   cccGACCGGCAGATCCATTTGCGCTACCCACTGTTCAAGCGCGCTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCATTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCg                                                                                 >  2:955157/1‑140 (MQ=255)                                                    ccGACCGGCAGATCCATTTGCGCTACCCACTGTTCAAGCGCGCTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCATTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGc                                                                                >  1:912605/1‑140 (MQ=255)                                                    ccGACCGGCAGATCCATTTGCGCTACCCACTGTTCAAGCGCGCTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCATTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGc                                                                                <  2:1101202/140‑1 (MQ=255)                                                        ctggCAGATCCATTTGCGCTACCCACTGTTCAAGCGCGCTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCATTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGt                                                                            <  1:231395/138‑1 (MQ=255)                                                                    tttGCGCTACCCACTGTTCAAGCGCGCTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCATTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCg                                                                <  2:912605/140‑1 (MQ=255)                                                                      tGCGCTACCCACTGTTCAAGCGCGCTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCATTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGc                                                              <  2:210654/140‑1 (MQ=255)                                                                      tGCGCTACCCACTGTTCAAGCGCGCTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCATTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGc                                                              >  1:1102048/1‑140 (MQ=255)                                                                      tGCGCTACCCACTGTTCAAGCGCGCTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCATTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGc                                                              >  2:1201968/1‑140 (MQ=255)                                                                      tGCGCTACCCACTGTTCAAGCGCGCTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCATTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGc                                                              >  2:1161541/1‑140 (MQ=255)                                                                      tGCGCTACCCACTGTTCAAGCGCGCTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCATTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGc                                                              <  2:180194/140‑1 (MQ=255)                                                                       gcgcTACCCACTGTTCAAGCGCGCTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCATTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCg                                                             <  1:195802/140‑1 (MQ=255)                                                                       gcgcTACCCACTGTTCAAGCGCGCTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCATTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCg                                                             <  2:114100/140‑1 (MQ=255)                                                                       gcgcTACCCACTGTTCAAGCGCGCTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCATTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCg                                                             <  1:1161541/140‑1 (MQ=255)                                                                       gcgcTACCCACTGTTCAAGCGCGCTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCATTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCg                                                             <  2:183414/140‑1 (MQ=255)                                                                          cTACCCACTGTTCAAGCGCGCTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCATTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTAt                                                          >  2:921891/1‑140 (MQ=255)                                                                            aCCCACTGTTCAAGCGCGCTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCATTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTg                                                        <  1:955157/140‑1 (MQ=255)                                                                            aCCCACTGTTCAAGCGCGCTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCATTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTg                                                        <  2:305191/140‑1 (MQ=255)                                                                            aCCCACTGTTCAAGCGCGCTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCATTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTg                                                        <  2:705881/140‑1 (MQ=255)                                                                             cccACTGTTCAAGCGCGCTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCATTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGc                                                       >  1:123127/1‑140 (MQ=255)                                                                                  tGTTCAAGCGCGCTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCATTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGt                                                  >  1:284084/1‑140 (MQ=255)                                                                                    ttcgaacgcgcTTAACGCGGGTTTGTTAGCTCGTAGCAACAGGCGAGTGGTGCATTTATCGACGCTCATACTCCCTTTGGCACTCAGTAGCGTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTta                                                >  2:1222102/7‑140 (MQ=255)                                                                                     tCAAGCGCGCTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCATTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTtat                                               >  1:910674/1‑140 (MQ=255)                                                                                       aaGCGCGCTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCATTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTg                                                        >  2:1056416/1‑129 (MQ=255)                                                                                       aaGCGCGCTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCATTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTg                                                        <  1:1056416/129‑1 (MQ=255)                                                                                       aaGCGCGCTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCATTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATAc                                             >  1:506115/1‑140 (MQ=255)                                                                                         gcgcgcTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCATTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCGTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTg                                           >  2:1073256/1‑140 (MQ=255)                                                                                            cgcTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCATTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGa                                        >  2:317658/1‑140 (MQ=255)                                                                                            cgcTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCATTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGa                                        >  1:549755/1‑140 (MQ=255)                                                                                             gcTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCATTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGAt                                       <  1:436451/140‑1 (MQ=255)                                                                                                      cGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCATTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCg                              <  2:506115/140‑1 (MQ=255)                                                                                                                  tcGTAGCAAAAGGCGATTGGTGCATTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCt                  <  2:613380/140‑1 (MQ=255)                                                                                                                  tcGTAACAAAAGGCGATTGGTGCATTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCt                  >  2:220624/1‑140 (MQ=255)                                                                                                                     tAGCAAAAGGCGATTGGTGCATTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTgcg               >  1:1027345/1‑140 (MQ=255)                                                                                                                     tAGCAAAAGGCGATTGGTGCATTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTgcg               >  1:745142/1‑140 (MQ=255)                                                                                                                       gCAAAAGGCGATTGGTGCATTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGcc             >  2:1143103/1‑140 (MQ=255)                                                                                                                          aaaGGCGATTGGTGCATTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCcgc          <  1:31478/140‑1 (MQ=255)                                                                                                                           aaGGCGATTGGTGCATTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCcgcg         >  1:713960/1‑140 (MQ=255)                                                                                                                            aGGCGATTGGTGCATTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCgcgc        >  1:163129/1‑140 (MQ=255)                                                                                                                            aGGCGATTGGTGCATTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCgcgc        >  2:94835/1‑140 (MQ=255)                                                                                                                            aGGCGATTGGTGCATTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCgcgc        >  1:364770/1‑140 (MQ=255)                                                                                                                            aGGCGATTGGTGCATTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCgcgc        <  2:284084/140‑1 (MQ=255)                                                                                                                             ggCGATTGGTGCATTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGcc       <  2:713960/140‑1 (MQ=255)                                                                                                                             ggCGATTGGTGCATTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGcc       <  1:1143103/140‑1 (MQ=255)                                                                                                                                gATTGGTGCATTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGc    >  2:940664/1‑140 (MQ=255)                                                                                                                                  ttGGTGCATTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTc  >  1:223264/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                     CGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCATTTGCGCTACCCACTGTTCAAGCGCGCTTAACGCCGTTTTGTTATCTCGTAGCAAAAGGCGATTGGTGCGTTTATCGACGGTCATACTCCCTTTGGCACTCAGTAGCTTCTCCCCCGCTTTCGCCAGTTCTCCCGCGTCGGCGTATTGCAGGGTTATACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTC  >  NC_000913/3520117‑3520385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑