Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,659,951	A→G	V240V (GTA→GTG)	mdtE →	multidrug efflux pump membrane fusion protein MdtE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,659,951	0	A	G	100.0%	254.5 / NA	71	V240V (GTA→GTG)	mdtE	multidrug efflux pump membrane fusion protein MdtE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (40/31);  total (40/31)

GGCAGATTCGCTGGTTACCGTACAACGTCTGGACCCGATTTATGTCGATCTCACGCAGTCGGTGCAAGATTTCTTACGCATGAAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTACAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTCCGACCCGACAGTGGATGAAACCACGGGCTCCGTGACGTTACGGGCGATTTTCCCCAACCCAAATGGTGACTTGCTGCC  >  NC_000913/3659816‑3660085                                                                                                                                        |                                                                                                                                       ggCAGATTCGCTGGTTACCGTACAACGTCTGGACCCGATTTATGTCGATCTCACGCAGTCGGTGCAAGATTTCTTACGCATGAAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGc                                                                                                                                    >  1:259606/1‑140 (MQ=255)  gCAGATTCGCTGGTTACCGTACAACGTCTGGACCCGATTTATGTCGATCTCACGCAGTCGGTGCAAGATTTCTTACGCATGAAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCt                                                                                                                                   <  2:45416/140‑1 (MQ=255)     gATTCGCTGGTTACCGTACAACGTCTGGACCCGATTTATGTCGATCTCACGCAGTCGGTGCAAGATTTCTTACGCATGAAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCaa                                                                                                                                >  1:814116/1‑140 (MQ=255)       ttCGCTGGTTACCGTACAACGTCTGGACCCGATTTATGTCGATCTCACGCAGTCGGTGCAAGATTTCTTACGCATGAAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATc                                                                                                                              >  2:221072/1‑140 (MQ=255)               ttACCGTACAACGTCTGGACCCGATTTATGTCGATCTCACGCAGTCGGTGCAAGATTTCTTACGCATGAAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAAt                                                                                                                      <  1:221072/140‑1 (MQ=255)                tACCGTACAACGTCTGGACCCGATTTATGTCGATCTCACGCAGTCGGTGCAAGATTTCTTACGCATGAAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATg                                                                                                                     >  2:30936/1‑140 (MQ=255)                tACCGTACAACGTCTGGACCCGATTTATGTCGATCTCACGCAGTCGGTGCAAGATTTCTTACGCATGAAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATg                                                                                                                     >  2:36753/1‑140 (MQ=255)                   cGTACAACGTCTGGACCCGATTTATGTCGATCTCACGCAGTCGGTGCAAGATTTCTTACGCATGAAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTa                                                                                                                  <  2:574279/140‑1 (MQ=255)                    gTACAACGTCTGGACCCGATTTATGTCGATCTCACGCAGTCGGTGCAAGATTTCTTACGCATGAAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTaa                                                                                                                 <  2:921276/140‑1 (MQ=255)                      aCAACGTCTGGACCCGATTTATGTCGATCTCACGCAGTCGGTGCAAGATTTCTTACGCATGAAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGCAAAc                                                                                                               >  2:719388/1‑140 (MQ=255)                        aaCGTCTGGACCCGATTTATGTCGATCTCACGCAGTCGGTGCAAGATTTCTTACGCATGAAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTa                                                                                                                  <  2:129283/135‑1 (MQ=255)                        aaCGTCTGGACCCGATTTATGTCGATCTCACGCAGTCGGTGCAAGATTTCTTACGCATGAAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTa                                                                                                                  >  1:129283/1‑135 (MQ=255)                         aCGTCTGGACCCGATTTATGTCGATCTCACGCAGTCGGTGCAAGATTTCTTACGCATGAAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCt                                                                                                            <  1:1080691/140‑1 (MQ=255)                          cGTCTGGACCCGATTTATGTCGATCTCACGCAGTCGGTGCAAGATTTCTTACGCATGAAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTa                                                                                                           >  2:445711/1‑140 (MQ=255)                            tCTGGACCCGATTTATGTCGATCTCACGCAGTCGGTGCAAGATTTCTTACGCATGAAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACa                                                                                                         >  2:909172/1‑140 (MQ=255)                            tCTGGACCCGATTTATGTCGATCTCACGCAGTCGGTGCAAGATTTCTTACGCATGAAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACa                                                                                                         >  1:1161509/1‑140 (MQ=255)                             cTGGACCCGATTTATGTCGATCTCACGCAGTCGGTGCAAGATTTCTTACGCATGAAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAg                                                                                                        >  2:494499/1‑140 (MQ=255)                               ggACCCGATTTATGTCGATCTCACGCAGTCGGTGCAAGATTTCTTACGCATGAAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGcc                                                                                                      >  1:1126063/1‑140 (MQ=255)                                      aTTTATGTCGATCTCACGCAGTCGGTGCAAGATTTCTTACGCATGAAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCgg                                                                                               >  2:1098764/1‑140 (MQ=255)                                      aTTTATGTCGATCTCACGCAGTCGGTGCAAGATTTCTTACGCATGAAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCgg                                                                                               <  1:30936/140‑1 (MQ=255)                                        ttATGTCGATCTCACGCAGTCGGTGCAAGATTTCTTACGCATGAAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCa                                                                                             <  2:137456/140‑1 (MQ=255)                                                   tcACGCAGTCGGTGCAAGATTTCTTACGCATGAAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATtc                                                                                  <  1:719388/140‑1 (MQ=255)                                                      cGCAGTCGGTGCAAGATTTCTTACGCATGAAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTcc                                                                               >  1:911931/1‑140 (MQ=255)                                                          gTCGGTGCAAGATTTCTTACGCATGAAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTccgacc                                                                           >  1:23531/1‑140 (MQ=255)                                                           tCGGTGCAAGATTTCTTACGCATGAAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATtctc                                                                                <  1:716252/134‑1 (MQ=255)                                                           tCGGTGCAAGATTTCTTACGCATGAAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATtctc                                                                                >  2:716252/1‑134 (MQ=255)                                                           tCGGTGCAAGATTTCTTACGCATGAAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTccgaccc                                                                          <  2:1190285/140‑1 (MQ=255)                                                             ggTGCAAGATTTCTTACGCATGAAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTccgacccga                                                                        <  1:325017/140‑1 (MQ=255)                                                             ggTGCAAGATTTCTTACGCATGAAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTccgacccga                                                                        <  1:1173775/140‑1 (MQ=255)                                                             ggTGCAAGATTTCTTACGCATGAAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTccgacccga                                                                        >  2:413425/1‑140 (MQ=255)                                                                    gATTTCTTACGCATGAAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTCCGACCCGACAGTGGa                                                                 <  1:445711/140‑1 (MQ=255)                                                                     aTTTCTTACGCATGAAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTCCGACCCGACAGTGGAt                                                                >  1:769350/1‑140 (MQ=255)                                                                      tttCTTACGCATGAAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTCCGACCCGACAGTGGATg                                                               <  2:463057/140‑1 (MQ=255)                                                                         cTTACGCATGAAAGAAGAGGTCGCCAGTGGGCAAATCAAGCAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTCCGACCCGACAGTGGATGaaa                                                            >  2:1167934/1‑140 (MQ=255)                                                                           tACGCATGAAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTccgacccga                                                                        >  2:325612/1‑126 (MQ=255)                                                                           tACGCATGAAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTccgacccga                                                                        <  1:325612/126‑1 (MQ=255)                                                                              gCATGAAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTCCGACCCGACAGTGGATGAAACCAc                                                        <  1:848415/139‑1 (MQ=255)                                                                              gCATGAAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTCCGACCCGACAGTGGATGAAACCAc                                                        >  2:848415/1‑139 (MQ=255)                                                                              gCATGAAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTCCGACCCGACAGTGGATGAAACCACg                                                       >  2:611162/1‑140 (MQ=255)                                                                                aTGAAAGAAGAGGTCGCCAGTGGGCAAATCAAGCAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTCCGACCCGACAGTGGATGAAACCACggg                                                     <  1:1167934/140‑1 (MQ=255)                                                                                      gaagaGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTCCGACCCGACAGTGGATGAAACCACGGGCTCCGt                                               <  2:815922/140‑1 (MQ=255)                                                                                        agagGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTCCGACCCGACAGTGGATGAAACCACGGGCTCCGTGa                                             >  2:175034/1‑140 (MQ=255)                                                                                         gagGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTCCGACCCGACAGTGGATGAAACCACGGGCTCCGTGAc                                            <  2:612372/140‑1 (MQ=255)                                                                                          agGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTCCGACCCGACAGTGGATGAAACCACGGGCTCCGTGACg                                           >  1:154330/1‑140 (MQ=255)                                                                                            gTCGCCAGTGGGCTAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTCCGACCCGACAGTGGATGAAACCACGGGCTCCGTGACGtt                                         >  2:1060469/1‑140 (MQ=255)                                                                                            gTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTCCGACCCGACAGTGGATGAAACCACGGGCTCCGTGACGtt                                         >  1:1014937/1‑140 (MQ=255)                                                                                            gTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTCCGACCCGACAGTGGATGAAACCACGGGCTCCGTGACGtt                                         >  2:680134/1‑140 (MQ=255)                                                                                            gTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTCCGACCCGACAGTGGATGAAACCACGGGCTCCGTGACGtt                                         >  2:706180/1‑140 (MQ=255)                                                                                            gTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTCCGACCCGACAGTGGATGAAACCACGGGCTCCGTGACGtt                                         >  1:896404/1‑140 (MQ=255)                                                                                            gTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTCCGACCCGACAGTGGATGAAACCACGGGCTCCGTGACGtt                                         <  2:769350/140‑1 (MQ=255)                                                                                            gTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTCCGACCCGACAGTGGATGAAACCACGGGCTCCGTGACGtt                                         >  1:446231/1‑140 (MQ=255)                                                                                            gTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTCCGACCCGACAGTGGATGAAACCACGGGCTCCGTGACGtt                                         >  1:1193755/1‑140 (MQ=255)                                                                                            gTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATGCTCCGACCCGACAGTGGATGAAACCACGGGCTCCGTGACGtt                                         >  2:1145431/1‑140 (MQ=255)                                                                                              cGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTCCGACCCGACAGTGGATGAAACCACGGGCTCCGTGACGTTAc                                       >  2:727245/1‑140 (MQ=255)                                                                                               gCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTCCGACCCGACAGTGGATGAAACCACGGGCTCCGTGACGTTACg                                      >  1:965523/1‑140 (MQ=255)                                                                                                      ggCAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTCCGACCCGACAGTGGATGAAACCACGGGCTCCGTGACGTTACGGGCGAtt                               <  2:911931/140‑1 (MQ=255)                                                                                                        cAAATCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTCCGACCCGACAGTGGATGAAACCACGGGCTCCGTGACGTTACGGGCGAtttt                             >  2:962403/1‑140 (MQ=255)                                                                                                            tCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTCCGACCCGACAGTGGATGAAACCACGGGCTCCGTGACGTTACGGGCGATTTTcccc                         <  2:896404/140‑1 (MQ=255)                                                                                                            tCAAACAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTCCGACCCGACAGTGGATGAAACCACGGGCTCCGTGACGTTACGGGCGATTTTcccc                         <  1:413425/140‑1 (MQ=255)                                                                                                                aCAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTCCGACCCGACAGTGGATGAAACCACGGGCTCCGTGACGTTACGGGCGATTTTCcccaacc                     <  2:446231/140‑1 (MQ=255)                                                                                                                aCAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTCCGACCCGACAGTGGATGAAACCACGGGCTCCGTGACGTTACGGGCGATTTTCcccaacc                     <  1:962403/140‑1 (MQ=255)                                                                                                                aCAGGTTCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTCCGACCCGACAGTGGATGAAACCACGGGCTCCGTGACGTTACGGGCGATTTTCcccaacc                     >  1:580834/1‑140 (MQ=255)                                                                                                                     ttCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACTCTGAAATTCTCCGACCCGACAGTGGATGAAACCACGGGCTCCGTGACGTTACGGGCGATTTTCcccaaccc                    <  2:858495/136‑1 (MQ=255)                                                                                                                     ttCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTCCGACCCGACAGTGGATGAAACCACGGGCTCCGTGACGTTACGGGCGATTTTCcccaaccc                    >  1:858495/1‑136 (MQ=255)                                                                                                                     ttCAGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTCCGACCCGACAGTGGATGAAACCACGGGCTCCGTGACGTTACGGGCGATTTTCCCCAACCCAAAt                >  1:635846/1‑140 (MQ=255)                                                                                                                        aGGGCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTCCGACCCGACAGTGGATGAAACCACGGGCTCCGTGACGTTACGGGCGATTTTCCCCAACCCAAATGGt             <  2:814116/140‑1 (MQ=255)                                                                                                                         gggCAGTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTCCGACCCGACAGTGGATGAAACCACGGGCTCCGTGACGTTACGGGCGATTTTCCCCAACCCAAATGGTg            <  2:965523/140‑1 (MQ=255)                                                                                                                              gTACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTCCGACCCGACAGTGGATGAAACCACGGGCTCCGTGACGTTACGGGCGATTTTCCCCAACCCAAATGGTGACTtg       >  1:738536/1‑140 (MQ=255)                                                                                                                               tACGCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTCCGACCCGACAGTGGATGAAACCACGGGCTCCGTGACGTTACGGGCGATTTTCCCCAACCCAAATGGTGACTtgc      >  2:661359/1‑140 (MQ=255)                                                                                                                                  gCCAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTCCGACCCGACAGTGGATGAAACCACGGGCTCCGTGACGTTACGGGCGATTTTCCCCAACCCAAATGGTGACTtgctgc   <  1:36753/140‑1 (MQ=255)                                                                                                                                   ccAGTGCAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTCCGACCCGACAGTGGATGAAACCACGGGCTCCGTGACGTTACGGGCGATTTTCCCCAACCCAAATGGTGACTTGCTGcc  <  2:1126063/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       GGCAGATTCGCTGGTTACCGTACAACGTCTGGACCCGATTTATGTCGATCTCACGCAGTCGGTGCAAGATTTCTTACGCATGAAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTACAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTCCGACCCGACAGTGGATGAAACCACGGGCTCCGTGACGTTACGGGCGATTTTCCCCAACCCAAATGGTGACTTGCTGCC  >  NC_000913/3659816‑3660085

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑