BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,114,380	(T)_7→6	intergenic (‑53/+122)	galF ← / ← wcaM	UTP:glucose‑1‑phosphate uridylyltransferase, low activity/putative colanic acid biosynthesis protein WcaM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,114,374	0	T	.	100.0%	460.6 / NA	108	intergenic (‑47/+128)	galF/wcaM	UTP:glucose‑1‑phosphate uridylyltransferase, low activity/putative colanic acid biosynthesis protein WcaM
Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (58/50); total (58/50)

Only 100 of 116 total aligned reads displayed.

AGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACATATGCATCCCGAGACCCGCTACAGGAATAACTGCTTTTAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGATTTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATCTACTCCC  >  NC_000913/2114242‑2114509

aGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACATATGCATCCCGAGACCCGCTACAGGAATAACTGCTTTTAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGAttttttcg                                                                                                                                  <  2:953316/140‑3 (MQ=255)

aGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACATATGCATCCCGAGACCCGCTACAGGAATAACTGCTTTTAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGAttttttcg                                                                                                                                  <  2:163587/140‑3 (MQ=255)

aGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACATATGCATCCCGAGACCCGCTACAGGAATAACTGCTTTCAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGAttttttcg                                                                                                                                  <  1:1291157/140‑3 (MQ=255)

 gCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACATATGCATCCCGAGACCCGCTACAGGAATAACTGCTTTTAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGAttttttcgc                                                                                                                                 <  1:1414582/140‑4 (MQ=255)

     ctctTTGGGTATCGCCTTAGTGGCAGGCAACATATGCATCCCGAGACCCGCTACAGGAATAACTGCTTTTAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGc                                                                                                                            <  1:1297953/140‑1 (MQ=255)

        tttGGGTATCGCCTTAGTGGCAGGCAACATATGCATCCCGAGACCCGCTACAGGAATAACTGCTTTTAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGAttttttcgc                                                                                                                                 >  2:51640/1‑130 (MQ=255)

        tttGGGTATCGCCTTAGTGGCAGGCAACATATGCATCCCGAGACCCGCTACAGGAATAACTGCTTTTAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGAttttttcgc                                                                                                                                 <  1:51640/133‑4 (MQ=255)

         ttGGGTATCGCCTTAGTGGCAGGCAACATATGCATCCCGAGACCCGCTACAGGAATAACTGCTTTTAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCa                                                                                                                        >  1:405184/1‑140 (MQ=255)

          tGGGTATCGCCTTAGTGGCAGGCAACATATGCATCCCGAGACCCGCTACAGGAATAACTGCTTTTAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCaa                                                                                                                       <  2:357105/140‑1 (MQ=255)

          tGGGTATCGCCTTAGTGGCAGGCAACATATGCATCCCGAGACCCGCTACAGGAATAACTGCTTTTAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCaa                                                                                                                       <  2:585755/140‑1 (MQ=255)

             gTATCGCCTTAGTGGCAGGCAACATATGCATCCCGAGACCCGCTACAGGAATAACTGCTTTTAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTAGA‑TTTTTTCGCCAGCATCAATTa                                                                                                                    <  1:76640/140‑1 (MQ=255)

                tCGCCTTAGTGGCAGGCAACATATGCATCCCGAGACCCGCTACAGGAATAACTGCTTTTAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGAttttttcg                                                                                                                                  >  1:1347384/1‑122 (MQ=255)

                tCGCCTTAGTGGCAGGCAACATATGCATCCCGAGACCCGCTACAGGAATAACTGCTTTTAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGAttttttcg                                                                                                                                  <  2:1347384/124‑3 (MQ=255)

                tCGCCTTAGTGGCAGGCAACATATGCATCCCGAGACCCGCTACAGGAATAACTGCTTTTAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTAccc                                                                                                                 >  2:645897/1‑140 (MQ=255)

                tCGCCTTAGTGGCAGGCAACATATGCATCCCGAGACCCGCTACAGGAATAACTGCTTTTAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTAccc                                                                                                                 >  2:1043097/1‑140 (MQ=255)

                tCGCCTTAGTGGCAGGCAACATATGCATCCCGAGACCCGCTACAGGAATAACTGCTTTTAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTAccc                                                                                                                 >  2:1474154/1‑140 (MQ=255)

                 cGCCTTAGTGGCAGGCAACATATGCATCCCGAGACCCGCTACAGGAATAACTGCTTTTAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCaa                                                                                                                       <  2:1004250/133‑1 (MQ=255)

                 cGCCTTAGTGGCAGGCAACATATGCATCCCGAGACCCGCTACAGGAATAACTGCTTTTAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCaa                                                                                                                       >  1:1004250/1‑133 (MQ=255)

                      tAGTGGCAGGCAACATATGCATCCCGAGACCCGCTACAGGAATAACTGCTTTTAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTAc                                                                                                                   <  1:1222649/132‑1 (MQ=255)

                      tAGTGGCAGGCAACATATGCATCCCGAGACCCGCTACAGGAATAACTGCTTTTAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTAc                                                                                                                   >  2:1222649/1‑132 (MQ=255)

                           gcaggcaACATATGCATCCCGAGACCCGCTACAGGAATAACTGCTTTTAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTa                                                                                                      <  1:1474154/140‑1 (MQ=255)

                              ggcaACATATGCATCCCGAGACCCGCTACAGGAATAACTGCTTTTAAATTCGTCATTGTTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTg                                                                                                   >  2:1326607/1‑140 (MQ=255)

                              ggcaACATATGCATCCCGAGACCCGCTACAGGAATAACTGCTTTTAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTg                                                                                                   >  2:1189267/1‑140 (MQ=255)

                              ggcaACATATGCATCCCGAGACCCGCTACAGGAATAACTGCTTGTAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTGCCCTGAATTGATTACTg                                                                                                   >  2:1315510/1‑140 (MQ=255)

                                    atatGCATCCCGAGACCCGCTACAGGAATAACTGCTTTTAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTAc                                                                                             <  1:214761/140‑1 (MQ=255)

                                     tatGCATCCCGAGACCCGCTACAGGAATAACTGCTTTTAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACt                                                                                            >  1:1285795/1‑140 (MQ=255)

                                       tGCATCCCGAGACCCGCTACAGGAATAACTGCTTTTAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTtg                                                                                          <  1:1315510/140‑1 (MQ=255)

                                        gCATCCCGAGACCCGCTACAGGAATAACTGCTTTTAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTtgt                                                                                         <  2:1194325/140‑1 (MQ=255)

                                        gCATCCCGAGACCCGCTACAGGAATAACTGCTTTTAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTtgt                                                                                         >  2:1145819/1‑140 (MQ=255)

                                           tCCCGAGACCCGCTACAGGAATAACTGCTTTTAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGAt                                                                                      >  2:1193919/1‑140 (MQ=255)

                                                agaCCCGCTACAGGAATAACTGCTTTTAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTAc                                                                                 >  2:1069436/1‑140 (MQ=255)

                                                  aCCCGCTACAGGAATAACTGCTTTTAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTAcgc                                                                               <  1:1193919/140‑1 (MQ=255)

                                                    ccGCTACAGGAATAACTGCTTTTAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTAcgccg                                                                             <  1:1352104/140‑1 (MQ=255)

                                                    ccGCTACAGGAATAACTGCTTTTAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTAcgccg                                                                             <  2:1114966/140‑1 (MQ=255)

                                                      gCTACAGGAATAACTGCTTTTAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCt                                                                           <  2:1054266/140‑1 (MQ=255)

                                                       cTACAGGAATAACTGCTTTTAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTa                                                                                  >  2:201781/1‑132 (MQ=255)

                                                       cTACAGGAATAACTGCTTTTAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTa                                                                                  <  1:201781/132‑1 (MQ=255)

                                                       cTACAGGAATAACTGCTTTTAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCtt                                                                          >  2:520943/1‑140 (MQ=255)

                                                              aaTAACTGCTTTTAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTtgtg                                                                   <  2:930508/140‑1 (MQ=255)

                                                                tAACTGCTTTTAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGa                                                                 <  1:1043097/140‑1 (MQ=255)

                                                                 aaCTGCTTTTAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTtgtg                                                                   <  2:302379/137‑1 (MQ=255)

                                                                 aaCTGCTTTTAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTtgtg                                                                   >  1:302379/1‑137 (MQ=255)

                                                                 aaCTGCTTTTAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGAt                                                                <  2:1285795/140‑1 (MQ=255)

                                                                 aaCTGCTTTTAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGAt                                                                <  1:1145819/140‑1 (MQ=255)

                                                                 aaCTGCTTTTAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGAt                                                                <  2:725830/140‑1 (MQ=255)

                                                                  aCTGCTTTTAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGAtt                                                               >  1:592131/1‑140 (MQ=255)

                                                                  aCTGCTTTTAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGAtt                                                               >  1:995620/1‑140 (MQ=255)

                                                                   cTGCTTTTAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTg                                                              <  1:195495/140‑1 (MQ=255)

                                                                    tGCTTTTAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGc                                                             >  1:649701/1‑140 (MQ=255)

                                                                    tGCTTTTAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGc                                                             >  2:985469/1‑140 (MQ=255)

                                                                     gCTTTTAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCt                                                                           >  1:518220/1‑125 (MQ=255)

                                                                     gCTTTTAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCt                                                                           <  2:518220/125‑1 (MQ=255)

                                                                      cTTTTAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAg                                                           <  2:19909/140‑1 (MQ=255)

                                                                       ttttAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGt                                                          >  2:432974/1‑140 (MQ=255)

                                                                        tttAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTa                                                         >  2:994492/1‑140 (MQ=255)

                                                                        tttAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTa                                                         >  1:389184/1‑140 (MQ=255)

                                                                        tttAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTa                                                         >  1:33704/1‑140 (MQ=255)

                                                                         ttAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAg                                                        <  1:483424/140‑1 (MQ=255)

                                                                           aaaTTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCa                                                      <  2:1035431/140‑1 (MQ=255)

                                                                           aaaTTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCa                                                      <  1:1069436/140‑1 (MQ=255)

                                                                           aaaTTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCa                                                      <  1:985469/140‑1 (MQ=255)

                                                                           aaaTTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCa                                                      <  1:1191366/140‑1 (MQ=255)

                                                                           aaaTTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCa                                                      <  2:1075415/140‑1 (MQ=255)

                                                                           aaaTTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCa                                                      <  2:33704/140‑1 (MQ=255)

                                                                             aTTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCAtt                                                    <  1:115166/140‑1 (MQ=255)

                                                                             aTTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCAtt                                                    <  2:389184/140‑1 (MQ=255)

                                                                                 gTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTc                                                >  2:757960/1‑140 (MQ=255)

                                                                                            aTCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGAc                                     >  2:1203021/1‑140 (MQ=255)

                                                                                             tCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACt                                    >  1:388548/1‑140 (MQ=255)

                                                                                             tCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACt                                    >  2:1300362/1‑140 (MQ=255)

                                                                                                aCCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCa                                 >  1:310176/1‑140 (MQ=255)

                                                                                                aCCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCa                                 >  2:290348/1‑140 (MQ=255)

                                                                                                 ccTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCAt                                <  2:405184/140‑1 (MQ=255)

                                                                                                 ccTGTAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCAt                                <  2:310176/140‑1 (MQ=255)

                                                                                                  ctatAAAATGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCAtt                               >  2:201357/4‑140 (MQ=255)

                                                                                                        aaTGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCa                         >  2:614522/1‑140 (MQ=255)

                                                                                                        aaTGGTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCa                         >  2:419364/1‑140 (MQ=255)

                                                                                                            gTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGaa                     >  2:681991/1‑140 (MQ=255)

                                                                                                            gTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGaa                     >  1:322157/1‑140 (MQ=255)

                                                                                                            gTTGCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGaa                     <  1:290348/140‑1 (MQ=255)

                                                                                                               gCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGa                      <  2:234554/136‑1 (MQ=255)

                                                                                                               gCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGa                      >  1:234554/1‑136 (MQ=255)

                                                                                                               gCTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATgg                  >  1:1062791/1‑140 (MQ=255)

                                                                                                                cTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGt                 <  1:1203021/140‑1 (MQ=255)

                                                                                                                cTGAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGt                 <  1:432974/140‑1 (MQ=255)

                                                                                                                  gAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCg               <  2:337303/140‑1 (MQ=255)

                                                                                                                  gAATTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCg               <  1:681991/140‑1 (MQ=255)

                                                                                                                    aTTATAGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCa             <  1:645897/140‑1 (MQ=255)

                                                                                                                        taGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATc         <  2:1361935/140‑1 (MQ=255)

                                                                                                                         aGCTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATCt        <  1:614522/140‑1 (MQ=255)

                                                                                                                           cTTGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATCTAc      <  1:633240/140‑1 (MQ=255)

                                                                                                                             tGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATCTACTc    >  1:441412/1‑140 (MQ=255)

                                                                                                                             tGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATCTACTc    >  1:926300/1‑140 (MQ=255)

                                                                                                                             tGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATCTACTc    >  2:1188652/1‑140 (MQ=255)

                                                                                                                             tGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATCTACTc    >  1:1061935/1‑140 (MQ=255)

                                                                                                                             tGTTCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATCTACTc    >  1:1061811/1‑140 (MQ=255)

                                                                                                                               ttCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATCTACTccc  >  1:766076/1‑140 (MQ=255)

                                                                                                                               ttCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATCTACTccc  <  1:1083359/140‑1 (MQ=255)

                                                                                                                               ttCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATCTACTccc  >  2:972259/1‑140 (MQ=255)

                                                                                                                               ttCGA‑TTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATCTACTccc  >  2:986711/1‑140 (MQ=255)

AGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACATATGCATCCCGAGACCCGCTACAGGAATAACTGCTTTTAAATTCGTCATTATTTCATCCACCTGTAAAATGGTTGCTGAATTATAGCTTGTTCGATTTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATCTACTCCC  >  NC_000913/2114242‑2114509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`