Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,313,965	Δ1 bp	coding (478/2673 nt)	rcsD →	RcsD phosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,313,964	0	A	.	81.7%	167.3 / 56.2	71	coding (477/2673 nt)	rcsD	RcsD phosphotransferase
Reads supporting (aligned to +/- strand):  ref base A (6/7);  new base . (33/25);  total (39/32)
Fisher's exact test for biased strand distribution p-value = 5.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.96e-01

GGCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTGCAGAGATGTTGCAACAGGCCAACGCCCTCGATGAACGCGAAAGCTTTTCTAACATGCGCCGC  >  NC_000913/2313830‑2314099                                                                                                                                       |                                                                                                                                        ggCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTC‑ACCCTa                                                                                                                                   >  2:966854/1‑140 (MQ=255) ggCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTC‑ACCCTa                                                                                                                                   <  1:966854/140‑1 (MQ=255)     ctcACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTC‑ACCCTAcccc                                                                                                                               >  2:372858/1‑140 (MQ=255)      tcACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTC‑ACCCTACCCCt                                                                                                                              >  2:828627/1‑140 (MQ=255)               cTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTC‑ACCCTACCCCTTAAAGAtct                                                                                                                     >  1:1039869/1‑140 (MQ=255)                  aaCGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTa                                                                                                                                   <  1:517638/124‑1 (MQ=255)                  aaCGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTa                                                                                                                                   >  2:517638/1‑124 (MQ=255)                    cGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTC‑ACCCTACCCCTTAAAGAtct                                                                                                                     >  2:1454809/1‑135 (MQ=255)                    cGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTC‑ACCCTACCCCTTAAAGAtct                                                                                                                     <  1:1454809/135‑1 (MQ=255)                    cGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCT‑AACCCTACCCCTTAAAGATCTCACCt                                                                                                                >  2:753471/1‑140 (MQ=255)                     gCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCT‑AACCCTACCCCTTAAAGATCTCACCTc                                                                                                               <  1:753471/140‑1 (MQ=255)                       ttGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCACCTcc                                                                                                              <  2:891372/140‑1 (MQ=255)                         gagaTGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAACGGTCAGGATAACAGTCTGGTGCTGATCTC‑ACCCTACCCCTTAAAGATCTCACCTCCGGa                                                                                                           >  2:1101531/1‑140 (MQ=255)                            aTGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTC‑ACCCTACCCCTTAAAGATCTCACCTCCGGAttt                                                                                                        >  2:337571/1‑140 (MQ=255)                               aCTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTC‑ACCCTACCCCTTAAAGATCTCACCTCCGGATTTaaa                                                                                                     <  1:676057/140‑1 (MQ=255)                                 tCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTC‑ACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGa                                                                                                   >  2:775454/1‑140 (MQ=255)                                     cGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTC‑ACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCg                                                                                               >  2:795246/1‑140 (MQ=255)                                      ggATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAA‑CCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGa                                                                                              <  2:445970/140‑1 (MQ=255)                                      ggATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTC‑ACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGa                                                                                              <  1:1319955/140‑1 (MQ=255)                                         tGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGAcc                                                                                            >  1:300940/1‑140 (MQ=255)                                                ttATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTC‑ACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGa                                                                                    <  1:1110214/140‑1 (MQ=255)                                                     tGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTC‑ACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACAttg                                                                               <  2:1397667/140‑1 (MQ=255)                                                     tGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTC‑ACCCTACCCCTT‑AAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACAttg                                                                               >  1:221810/1‑139 (MQ=255)                                                     tGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTC‑ACCCTACCCCTT‑AAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACAttg                                                                               <  2:221810/139‑1 (MQ=255)                                                          aCATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTC‑ACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGAt                                                                          >  2:1319942/1‑140 (MQ=255)                                                           cATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTC‑ACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGAtt                                                                         <  1:1319942/140‑1 (MQ=255)                                                            aTTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTC‑ACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTc                                                                        <  1:372858/140‑1 (MQ=255)                                                               gtgGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTC‑ACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCAcg                                                                     <  1:775454/140‑1 (MQ=255)                                                               gtgGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTC‑ACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCAcg                                                                     <  1:337571/140‑1 (MQ=255)                                                               gtgGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTC‑ACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCAcg                                                                     <  2:1039869/140‑1 (MQ=255)                                                               gtgGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTC‑ACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCAcg                                                                     <  1:957424/140‑1 (MQ=255)                                                                tgGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTC‑ACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCAcgt                                                                    <  1:828627/140‑1 (MQ=255)                                                                 ggggCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTC‑ACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCAcgtc                                                                   >  2:1203584/1‑140 (MQ=255)                                                                   ggCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTC‑ACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCAcgtcgt                                                                 >  1:1222821/1‑140 (MQ=255)                                                                     cgcAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTC‑ACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCAcg                                                                     >  1:1148117/1‑134 (MQ=255)                                                                     cgcAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTC‑ACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCAcg                                                                     <  2:1148117/134‑1 (MQ=255)                                                                      gcAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTC‑ACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTGCa                                                              >  1:95911/1‑140 (MQ=255)                                                                      gcAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTC‑ACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTGCa                                                              >  1:388599/1‑140 (MQ=255)                                                                       cAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTC‑ACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTGCag                                                             >  1:861178/1‑140 (MQ=255)                                                                       cAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTC‑ACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTGCag                                                             >  2:1029284/1‑140 (MQ=255)                                                                       cAGAAAATGTACCGTGGTCGATGTATTACCTGAACGGTCAGGATAACAGTCTGGTGCTGATCTC‑ACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTGCag                                                             <  1:1101531/140‑1 (MQ=255)                                                                        aGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTC‑ACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTGCaga                                                            <  2:1222821/140‑1 (MQ=255)                                                                        aGAAAATGTACCGTGGGCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTC‑ACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTGCaga                                                            <  2:1294937/140‑1 (MQ=255)                                                                                    gTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTGCAGAGATGTTGCAAc                                                 >  1:1347138/1‑140 (MQ=255)                                                                                      ggTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTGCAGAGATGt                                                       >  2:1318338/1‑132 (MQ=255)                                                                                      ggTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTGCAGAGATGt                                                       <  1:1318338/132‑1 (MQ=255)                                                                                          gATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTC‑ACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTGCAGAGATGTTGCAACAGGCCaa                                          >  2:1034076/1‑140 (MQ=255)                                                                                          gATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTC‑ACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTGCAGAGATGTTGCAACAGGCCaa                                          <  1:795246/140‑1 (MQ=255)                                                                                           aTGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTTAGCGACATTGTTGATTCACGTCGTGCAGAGATGTTGCAACAGGCCaa                                          <  2:300940/140‑1 (MQ=255)                                                                                            tGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTC‑ACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTGCAGAGATGTTGCAACAGGCCAACg                                        <  1:994690/140‑1 (MQ=255)                                                                                                  aCCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTC‑ACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTGCAGAGATGTTGCAACAGGCCAACGCCCTCg                                  >  2:34000/1‑140 (MQ=255)                                                                                                      gAATGGTCAGGATAACAGTCTGGTGCTGATCTC‑ACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTGCAGAGATGTTGCAACAGGCCAACGCCCTCGATGa                              >  2:948917/1‑140 (MQ=255)                                                                                                      gAATGGACAGGATAACAGTCTGGTGCTGATCTC‑ACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTGCAGAGATGTTGCAACAGGCCAACGCCCTCGATGa                              >  1:1104418/1‑140 (MQ=255)                                                                                                               ggATAACAGTCTGGTGCTGATCTC‑ACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTGCAGAGATGTTGCAACAGGCCAACGCCCTCGATGAACGCGAAAg                     <  1:1029284/140‑1 (MQ=255)                                                                                                               ggATAACAGTCTGGTGCTGATCTC‑ACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTGCAGAGATGTTGCAACAGGCCAACGCCCTCGATGAACGCGAAAg                     <  2:17503/140‑1 (MQ=255)                                                                                                                gATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTGCAGAGATGTTGCAACAGGCCAACGCCCTCGATGAACGCGAAAg                     <  1:407862/140‑1 (MQ=255)                                                                                                                    aCAGTCTGGTGCTGATCTC‑ACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTGCAGAGATGTTGCAACAGGCCAACGCCCTCGATGAACGCGAAAGCtttt                <  1:948917/140‑1 (MQ=255)                                                                                                                        tCTGGTGCTGATCTC‑ACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTGCAGAGATGTTGCAACAGGCCAACGCCCTCGATGAACGCGAAAGCTTTTCTaa            >  1:443865/1‑140 (MQ=255)                                                                                                                          tGGTGCTGATCTC‑ACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTGCAGAGATGTTGCAACAGGCCAACGCCCTCGATGAGCGCGAAAGCTTTTCTAACa          >  2:1317326/1‑140 (MQ=255)                                                                                                                          tGGTGCTGATCTC‑ACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTGCAGAGATGTTGCAACAGGCCAACGCCCTCGATGAACGCGAAAGCTTTTCTAACa          >  1:992328/1‑140 (MQ=255)                                                                                                                          tGGTGCTGATCTC‑ACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTGCAGAGATGTTGCAACAGGCCAACGCCCTCGATGAACGCGAAAGCTTTTCTAACa          >  2:1330800/1‑140 (MQ=255)                                                                                                                          tGGTGCTGATCTC‑ACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTGCAGAGATGTTGCAACAGGCCAACGCCCTCGATGAACGCGAAAGCTTTTCTAACa          >  1:304453/1‑140 (MQ=255)                                                                                                                          tGGTGCTGATCTC‑ACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTGCAGAGATGTTGCAACAGGCCAACGCCCTCGATGAACGCGAAAGCTTTTCTAACa          >  1:1103035/1‑140 (MQ=255)                                                                                                                             tGCTGATCTCAACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTGCAGAGATGTTGCAACAGGCCAACGCCCTCGATGAACGCGAAAGCTTTTCTAACATg        >  2:1082314/1‑140 (MQ=255)                                                                                                                             tGCTGATCTC‑ACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTGCAGAGATGTTGCAACAGGCCAACGCCCTCGATGAACGCGAAAGCTTTTCTAACATgc       >  2:125811/1‑140 (MQ=255)                                                                                                                             tGCTGATCTC‑ACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTGCAGAGATGTTGCAACAGGCCAACGCCCTCGATGAACGCGAAAGCTTTTCTAACATgc       <  1:1334802/140‑1 (MQ=255)                                                                                                                             tGCTGATCTC‑ACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTGCAGAGATGTTGCAACAGGCCAACGCCCTCGATGAACGCGAAAGCTTTTCTAACATgc       <  1:34000/140‑1 (MQ=255)                                                                                                                             tGCTGATCTC‑ACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTGCAGAGATGTTGCAACAGGCCAACGCCCTCGATGAACGCGAAAGCTTTTCTAACATgc       >  2:1334802/1‑140 (MQ=255)                                                                                                                               cTGATCTC‑ACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTGCAGAGATGTTGCAACAGGCCAACGCCCTCGATGAACGCGAAAGCTTTTCTAACATgcgc     >  1:918733/1‑140 (MQ=255)                                                                                                                               cTGATCTC‑ACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTGCAGAGATGTTGCAACAGGCCAACGCCCTCGATGAACGCGAAAGCTTTTCTAACATgcgc     >  2:189677/1‑140 (MQ=255)                                                                                                                                 gATCTC‑ACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTGCAGAGATGTTGCAACAGGCCAACGCCCTCGATGAACGCGAAAGCTTTTCTAACATGcgccg   >  2:21289/1‑140 (MQ=255)                                                                                                                                  aTCTC‑ACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTGCAGAGATGTTGCAACAGGCCAACGCCCTCGATGAACGCGAAAGCTTTTCTAACATGcgccgc  >  2:1066211/1‑140 (MQ=255)                                                                                                                                  aTCTC‑ACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTGCAGAGATGTTGCAACAGGCCAACGCCCTCGATGAACGCGAAAGCTTTTCTAACATGcgccgc  >  1:27514/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                        GGCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATCTGGATACATTGTGGGGCGCAGAAAATGTACCGTGGTCGATGTATTACCTGAATGGTCAGGATAACAGTCTGGTGCTGATCTCAACCCTACCCCTTAAAGATCTCACCTCCGGATTTAAAGAATCGACCGTCAGCGACATTGTTGATTCACGTCGTGCAGAGATGTTGCAACAGGCCAACGCCCTCGATGAACGCGAAAGCTTTTCTAACATGCGCCGC  >  NC_000913/2313830‑2314099

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑