Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,407,333	(A)7→6	intergenic (‑692/‑228)	lrhA ← / → alaA	DNA‑binding transcriptional dual regulator LrhA/glutamate‑‑pyruvate aminotransferase AlaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,407,327	0	A	.	100.0%	392.2 / NA	92	intergenic (‑686/‑234)	lrhA/alaA	DNA‑binding transcriptional dual regulator LrhA/glutamate‑‑pyruvate aminotransferase AlaA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (40/52);  total (40/52)

AGTTTCACACCTTGCCCCCATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTA  >  NC_000913/2407191‑2407462                                                                                                                                         |                                                                                                                                        aGTTTCACACCTTGCCCCCATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATaaaa                                                                                                                                      <  2:935177/140‑1 (MQ=255)    ttCACACCTTGCCCCCATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATaaaaaac                                                                                                                                   <  2:1471574/140‑2 (MQ=255)         acCTTGCCCCCATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGAc                                                                                                                             <  1:1270007/140‑1 (MQ=255)         acCTTGCCCCCATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGAc                                                                                                                             <  1:263281/140‑1 (MQ=255)          ccTTGCCCCCATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACt                                                                                                                            <  2:1004456/140‑1 (MQ=255)           cTTGCCCCCATTGCGACACCACGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACtt                                                                                                                           >  1:841751/1‑140 (MQ=255)            ttGCCCCCATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTa                                                                                                                          >  2:1476481/1‑140 (MQ=255)            ttGCCCCCATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTa                                                                                                                          >  1:1336948/1‑140 (MQ=255)             tGCCCCCATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTAc                                                                                                                         >  1:1199121/1‑140 (MQ=255)               cccccATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACaa                                                                                                                       >  2:1291853/1‑140 (MQ=255)                    aTTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAg                                                                                                                  >  1:179941/1‑140 (MQ=255)                     ttGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGa                                                                                                                 >  2:694130/1‑140 (MQ=255)                     ttGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCATAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGa                                                                                                                 >  2:1274209/1‑140 (MQ=255)                       gCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAAt                                                                                                               <  2:102173/140‑1 (MQ=255)                       gCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATCAAGAAt                                                                                                               <  1:783520/140‑1 (MQ=255)                         gACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCa                                                                                                             <  1:973872/140‑1 (MQ=255)                          acacCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAg                                                                                                            >  2:602176/1‑140 (MQ=255)                            acCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGaa                                                                                                          <  2:724056/140‑1 (MQ=255)                              cTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTa                                                                                                                          >  2:361806/1‑122 (MQ=255)                              cTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTa                                                                                                                          <  1:361806/122‑1 (MQ=255)                              cTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACa                                                                                                        >  1:53025/1‑140 (MQ=255)                              cTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACa                                                                                                        >  2:346069/1‑140 (MQ=255)                                cGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTa                                                                                                                    >  1:625937/1‑126 (MQ=255)                                cGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTa                                                                                                                    <  2:625937/126‑1 (MQ=255)                                cGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAAt                                                                                                      >  2:1133801/1‑140 (MQ=255)                                 ggAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAAtt                                                                                                     <  2:914347/140‑1 (MQ=255)                                 ggAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAAtt                                                                                                     <  1:602176/140‑1 (MQ=255)                                 ggAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAAtt                                                                                                     <  1:499701/140‑1 (MQ=255)                                 ggAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAAtt                                                                                                     <  1:446287/140‑1 (MQ=255)                                 ggAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAAtt                                                                                                     <  1:398577/140‑1 (MQ=255)                                 ggAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAAtt                                                                                                     <  2:1199121/140‑1 (MQ=255)                                 ggAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAAtt                                                                                                     <  2:1336948/140‑1 (MQ=255)                                 ggAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTGACAATTAAGAATCAGAACAAtt                                                                                                     <  2:546399/140‑1 (MQ=255)                                   aaaTCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCa                                                                                                   <  1:694130/140‑1 (MQ=255)                                     atcatcACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGGCTTACAATTAAGAATCAGAACAATTCACt                                                                                                 <  1:1275844/140‑1 (MQ=255)                                     atcatcACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACt                                                                                                 <  1:1291853/140‑1 (MQ=255)                                     atcatcACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACt                                                                                                 <  2:1066590/140‑1 (MQ=255)                                       catcaCGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACtat                                                                                               >  1:1299381/1‑140 (MQ=255)                                        atcaCGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACtata                                                                                              <  1:550494/140‑1 (MQ=255)                                        atcaCGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACtata                                                                                              <  1:1133801/140‑1 (MQ=255)                                              cAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACAt                                                                                        <  2:768047/140‑1 (MQ=255)                                               aGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACATAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACAtt                                                                                       <  2:1299381/140‑1 (MQ=255)                                                  tAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAAtt                                                                                                     <  2:129604/123‑1 (MQ=255)                                                  tAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAAtt                                                                                                     >  1:129604/1‑123 (MQ=255)                                                     aCTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGATTCAGAACAATTCACTATATAACATTGCATGt                                                                                 <  2:1431393/140‑1 (MQ=255)                                                     aCTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGt                                                                                 <  1:346069/140‑1 (MQ=255)                                                     aCTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGt                                                                                 <  2:932666/140‑1 (MQ=255)                                                        ctcCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTaaa                                                                              <  1:1476481/140‑1 (MQ=255)                                                        ctcCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTaaa                                                                              <  2:1445937/140‑1 (MQ=255)                                                        ctcCATAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTaaa                                                                              <  1:1274209/140‑1 (MQ=255)                                                         tcCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAg                                                                             >  1:25853/1‑140 (MQ=255)                                                           cGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACAt                                                                                        >  2:716062/1‑127 (MQ=255)                                                           cGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACAt                                                                                        <  1:716062/127‑1 (MQ=255)                                                           cGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTaaa                                                                              <  1:158029/137‑1 (MQ=255)                                                           cGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTaaa                                                                              >  2:158029/1‑137 (MQ=255)                                                           cGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTaaa                                                                              >  2:543383/1‑137 (MQ=255)                                                           cGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTaaa                                                                              <  1:543383/137‑1 (MQ=255)                                                           cGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTaa                                                                               >  2:285195/1‑136 (MQ=255)                                                           cGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTaa                                                                               <  1:285195/136‑1 (MQ=255)                                                           cGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCa                                                                           >  1:194567/1‑140 (MQ=255)                                                                 gAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATaca                                                                     <  2:179941/140‑1 (MQ=255)                                                                   ggAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACAcc                                                                   >  2:1376810/1‑140 (MQ=255)                                                                     aaaTATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTaaa                                                                              >  2:1219202/1‑127 (MQ=255)                                                                     aaaTATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTaaa                                                                              <  1:1219202/127‑1 (MQ=255)                                                                     aaaTATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTc                                                                 <  2:841751/140‑1 (MQ=255)                                                                       atatCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTaa                                                                               >  1:168598/1‑124 (MQ=255)                                                                       atatCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTaa                                                                               <  2:168598/124‑1 (MQ=255)                                                                            atcatcGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTAttt                                                          >  2:675828/1‑140 (MQ=255)                                                                            atcatcGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTAttt                                                          >  1:1070893/1‑140 (MQ=255)                                                                            atcatcGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTAttt                                                          >  1:1259174/1‑140 (MQ=255)                                                                               atcGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGt                                                       >  1:208063/1‑140 (MQ=255)                                                                               atcGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGt                                                       >  1:768968/1‑140 (MQ=255)                                                                               atcGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGt                                                       <  2:666956/140‑1 (MQ=255)                                                                                 cgcgTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATaca                                                                     <  1:65325/124‑1 (MQ=255)                                                                                 cgcgTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATaca                                                                     >  2:65325/1‑124 (MQ=255)                                                                                  gcgTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCat                                                    >  1:170746/1‑140 (MQ=255)                                                                                   cgTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCatt                                                   >  2:453954/1‑140 (MQ=255)                                                                                    gTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCatta                                                  <  2:53025/140‑1 (MQ=255)                                                                                    gTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCatta                                                  <  1:398249/140‑1 (MQ=255)                                                                                      ttCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCattatt                                                <  2:1070893/140‑1 (MQ=255)                                                                                           tAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGtt                                           <  1:453954/140‑1 (MQ=255)                                                                                                aaTTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTaa                                      >  1:943717/1‑140 (MQ=255)                                                                                                  ttCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACa                                    >  1:171019/1‑140 (MQ=255)                                                                                                    cTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGc                                  <  2:1259174/140‑1 (MQ=255)                                                                                                     ttAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGca                                 <  1:1063044/140‑1 (MQ=255)                                                                                                        aaTGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGca                                 >  2:1240453/1‑137 (MQ=255)                                                                                                        aaTGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGca                                 <  1:1240453/137‑1 (MQ=255)                                                                                                         aTGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAAt                             <  2:171019/140‑1 (MQ=255)                                                                                                          tGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATc                            <  2:1256232/140‑1 (MQ=255)                                                                                                             ggCATTTTCACACAATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAg                         >  1:720898/1‑140 (MQ=255)                                                                                                                        acaATTATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCctct              >  2:1322815/1‑140 (MQ=255)                                                                                                                            ttATCTTACAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCa          >  2:764015/1‑140 (MQ=255)                                                                                                                            ttATCTTACAATCAAAAAATCCAGCCGTACAATTAACCATCAGAACACTTCACTATAGAACATTTCATGCAAAGCATAGATGACACGTTATTTACCCATTATTAAGTGATTAACAGCATAATCGAGCCTTCCCCTCTgac           >  2:426220/1‑138 (MQ=255)                                                                                                                                    cAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTa  <  2:170746/140‑1 (MQ=255)                                                                                                                                    cAGAT‑AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTa  >  2:684703/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        AGTTTCACACCTTGCCCCCATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTA  >  NC_000913/2407191‑2407462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑