Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,002,224	(G)7→6	coding (418/1071 nt)	elfG →	putative fimbrial‑like adhesin protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,002,218	0	G	.	100.0%	313.1 / NA	74	coding (412/1071 nt)	elfG	putative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (39/35);  total (39/35)

TGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGGTCGTCATTC  >  NC_000913/1002080‑1002344                                                                                                                                           |                                                                                                                               tGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAgg                                                                                                                               <  2:1025434/140‑1 (MQ=255)     cATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAgggggg                                                                                                                           >  2:496338/1‑140 (MQ=255)                           aGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGa                                                                                                    >  1:1163264/1‑140 (MQ=255)                           aGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGa                                                                                                    >  2:613843/1‑140 (MQ=255)                           aGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGa                                                                                                    >  1:138560/1‑140 (MQ=255)                               tATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCt                                                                                                >  2:493025/1‑140 (MQ=255)                                   tAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCg                                                                                            <  1:613843/140‑1 (MQ=255)                                     ggCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGt                                                                                          <  2:65663/140‑1 (MQ=255)                                     ggCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGt                                                                                          <  1:496338/140‑1 (MQ=255)                                     ggCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGt                                                                                          <  1:1471747/140‑1 (MQ=255)                                      gCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGt                                                                                          >  2:290469/1‑139 (MQ=255)                                      gCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGt                                                                                          <  1:290469/139‑1 (MQ=255)                                       cGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCa                                                                                        >  1:631731/1‑140 (MQ=255)                                       cGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCa                                                                                        >  2:585053/1‑140 (MQ=255)                                         aCAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAAc                                                                                      >  2:642142/1‑140 (MQ=255)                                         aCAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAAc                                                                                      >  2:262553/1‑140 (MQ=255)                                         aCAACCGGTACAAAATTAATGACTATCTTGCCTCCAAAGTCTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGCGATACCCGATGGCATATTTGCGACCCTGGCGGTCAAc                                                                                      <  2:1305613/140‑1 (MQ=255)                                           aaCTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGc                                                                                             >  2:1297570/1‑131 (MQ=255)                                           aaCTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGc                                                                                             <  1:1297570/131‑1 (MQ=255)                                           aaCTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGt                                                                                    >  1:391105/1‑140 (MQ=255)                                           aaCTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGt                                                                                    >  2:1223181/1‑140 (MQ=255)                                           aaCTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGt                                                                                    >  1:1305613/1‑140 (MQ=255)                                           aaCTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGt                                                                                    >  1:142952/1‑140 (MQ=255)                                               ggTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAg                                                                                <  1:585053/140‑1 (MQ=255)                                                gTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGt                                                                                          <  2:1341560/129‑1 (MQ=255)                                                gTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGt                                                                                          >  1:1341560/1‑129 (MQ=255)                                                 tACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGt                                                                              <  1:441321/140‑1 (MQ=255)                                                  aCAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTg                                                                             >  2:129453/1‑140 (MQ=255)                                                   cAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTgg                                                                            >  2:273317/1‑140 (MQ=255)                                                    aaaaTTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTggg                                                                           >  1:1018406/1‑140 (MQ=255)                                                    aaaaTTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTggg                                                                           <  1:273317/140‑1 (MQ=255)                                                     aaaTTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGc                                                                          >  2:655586/1‑140 (MQ=255)                                                      aaTTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCa                                                                         <  2:138560/140‑1 (MQ=255)                                                           aTGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGtt                                                                                   >  1:711061/1‑125 (MQ=255)                                                           aTGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGtt                                                                                   <  2:711061/125‑1 (MQ=255)                                                           aTGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAggggg                                                                    <  1:262553/140‑1 (MQ=255)                                                           aTGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAggggg                                                                    <  1:493025/140‑1 (MQ=255)                                                             gACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCa                                                                  <  1:595762/140‑1 (MQ=255)                                                             gACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCa                                                                  <  1:1454973/140‑1 (MQ=255)                                                             gACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCa                                                                  <  1:1361040/140‑1 (MQ=255)                                                             gACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCa                                                                  <  1:129453/140‑1 (MQ=255)                                                                tATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGc                                                               >  2:405095/1‑140 (MQ=255)                                                                tATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTCCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGc                                                               >  1:286344/1‑140 (MQ=255)                                                                  tCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCgg                                                             >  1:899113/1‑140 (MQ=255)                                                                  tCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCgg                                                             >  2:66571/1‑140 (MQ=255)                                                                  tCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCgg                                                             >  1:68808/1‑140 (MQ=255)                                                                    ttGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTa                                                           <  2:391105/140‑1 (MQ=255)                                                                     tGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTaa                                                          <  1:655586/140‑1 (MQ=255)                                                                      gccgccAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAAt                                                         <  2:1018406/140‑1 (MQ=255)                                                                       ccgccAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAggggg                                                                    >  2:499865/1‑128 (MQ=255)                                                                       ccgccAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAggggg                                                                    <  1:499865/128‑1 (MQ=255)                                                                          ccAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCg                                                     <  2:76615/140‑1 (MQ=255)                                                                          ccAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCg                                                     >  1:1474670/1‑140 (MQ=255)                                                                            aaaGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGt                                                   >  1:220132/1‑140 (MQ=255)                                                                                    aTTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTc                                           <  2:989885/140‑1 (MQ=255)                                                                                       gCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCt                                        <  2:698427/140‑1 (MQ=255)                                                                                       gCAGGTTAAAGGCAGTCCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCt                                        <  2:286344/140‑1 (MQ=255)                                                                                          ggTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGaa                                     >  2:888433/1‑140 (MQ=255)                                                                                          ggTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGaa                                     >  1:904085/1‑140 (MQ=255)                                                                                                   cAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCa                            <  2:220132/140‑1 (MQ=255)                                                                                                     gTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGc                          >  2:1266124/1‑140 (MQ=255)                                                                                                      ttCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑‑GGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCcg                        >  2:201227/1‑140 (MQ=255)                                                                                                                gCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGc               >  2:1094351/1‑140 (MQ=255)                                                                                                                gCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTAAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGc               >  2:147848/1‑140 (MQ=255)                                                                                                                gCGGTTCCTTTAGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGc               >  2:1258818/1‑140 (MQ=255)                                                                                                                 cGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCt              <  2:904085/140‑1 (MQ=255)                                                                                                                   gTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGc                          >  1:1140946/1‑126 (MQ=255)                                                                                                                   gTTCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGc                          <  2:1140946/126‑1 (MQ=255)                                                                                                                    ttCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCgg           >  2:1050669/1‑140 (MQ=255)                                                                                                                     tCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGgt          <  2:542645/140‑1 (MQ=255)                                                                                                                     tCCTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGgt          <  1:1266124/140‑1 (MQ=255)                                                                                                                      ccTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGgtc         <  1:405095/140‑1 (MQ=255)                                                                                                                      ccTTTCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCAGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGgtc         >  2:143932/1‑140 (MQ=255)                                                                                                                       cTTTAGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGgtcg        <  1:1258818/140‑1 (MQ=255)                                                                                                                         ttCGAAAACGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGgtcgtc      >  1:266113/1‑140 (MQ=255)                                                                                                                             aaaaCGTGGGCACA‑GGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGGTCGTCATtc  <  2:266113/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                               TGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGGTCGTCATTC  >  NC_000913/1002080‑1002344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑