BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,263,632	(G)_5→4	intergenic (‑132/‑82)	lolB ← / → hemA	outer membrane lipoprotein LolB/glutamyl‑tRNA reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,263,628	0	G	.	100.0%	386.8 / NA	91	intergenic (‑128/‑86)	lolB/hemA	outer membrane lipoprotein LolB/glutamyl‑tRNA reductase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (44/47); total (44/47)

Only 100 of 108 total aligned reads displayed.

GGGGCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCGGTATCAACCATAAAACGG  >  NC_000913/1263494‑1263750

ggggCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAggggcg                                                                                                                       <  2:286506/140‑3 (MQ=255)

ggggCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAggggcg                                                                                                                       <  1:860740/140‑3 (MQ=255)

ggggCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAggggcg                                                                                                                       <  1:927390/140‑3 (MQ=255)

ggggCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAggggcg                                                                                                                       <  2:531949/140‑3 (MQ=255)

ggggCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAggggcg                                                                                                                       <  2:744184/140‑3 (MQ=255)

  ggCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAggggcgct                                                                                                                     <  1:331934/140‑5 (MQ=255)

  ggCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAggggcgct                                                                                                                     <  2:1089815/140‑5 (MQ=255)

  ggCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAggggcgct                                                                                                                     <  2:1394426/140‑5 (MQ=255)

  ggCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAggggcgct                                                                                                                     <  2:464475/140‑5 (MQ=255)

   gCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAggggcg                                                                                                                       >  1:1246034/1‑135 (MQ=255)

    cATAGTGATGTCAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGctct                                                                                                                  <  2:552601/140‑1 (MQ=255)

      tAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCAATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAggggcg                                                                                                                       >  1:1172687/1‑132 (MQ=255)

      tAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCAATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAggggcg                                                                                                                       <  2:1172687/134‑3 (MQ=255)

           atgaCAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAggggcgct                                                                                                                     <  1:1431555/131‑5 (MQ=255)

           atgaCAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAggggcgct                                                                                                                     >  2:1431555/1‑127 (MQ=255)

           atgaCAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTAt                                                                                                           <  1:1038258/140‑1 (MQ=255)

           atgaCAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTAt                                                                                                           <  2:514523/140‑1 (MQ=255)

            tgaCAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTAtt                                                                                                          >  2:114590/1‑140 (MQ=255)

              aCAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTAGGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGa                                                                                                        >  2:1298764/1‑140 (MQ=255)

                  gTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCtt                                                                                                    >  1:238684/1‑140 (MQ=255)

                   tCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAggggcgct                                                                                                                     <  1:1369444/123‑5 (MQ=255)

                   tCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAggggcgct                                                                                                                     >  2:1369444/1‑119 (MQ=255)

                   tCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTa                                                                                                   >  1:451193/1‑140 (MQ=255)

                   tCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTa                                                                                                   <  1:95787/140‑1 (MQ=255)

                    ccTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTAc                                                                                                  <  1:582969/140‑1 (MQ=255)

                    ccTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTAc                                                                                                  <  1:329243/140‑1 (MQ=255)

                    ccTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTAc                                                                                                  <  2:1455335/140‑1 (MQ=255)

                     cTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACg                                                                                                 <  1:739306/140‑1 (MQ=255)

                     cTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACg                                                                                                 >  1:1107256/1‑140 (MQ=255)

                     cTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACg                                                                                                 >  1:994396/1‑140 (MQ=255)

                       tGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCa                                                                                               <  2:1435762/140‑1 (MQ=255)

                       tGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCa                                                                                               >  2:665206/1‑140 (MQ=255)

                       tGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCa                                                                                               >  2:143544/1‑140 (MQ=255)

                             aCGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACg                                                                                                 <  2:664743/132‑1 (MQ=255)

                             aCGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACg                                                                                                 >  1:664743/1‑132 (MQ=255)

                             aCGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGt                                                                                         >  2:1241984/1‑140 (MQ=255)

                             aCGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGt                                                                                         >  2:258142/1‑140 (MQ=255)

                             aCGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGt                                                                                         <  1:1298764/140‑1 (MQ=255)

                             aCGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGt                                                                                         >  2:982657/1‑140 (MQ=255)

                             aCGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGt                                                                                         <  1:1241984/140‑1 (MQ=255)

                             aCGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGt                                                                                         >  1:1465970/1‑140 (MQ=255)

                              cGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTa                                                                                        >  2:647495/1‑140 (MQ=255)

                               gTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTat                                                                                       >  2:352246/1‑140 (MQ=255)

                                 tGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTa                                                                                                   >  1:758169/1‑126 (MQ=255)

                                 tGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTa                                                                                                   <  2:758169/126‑1 (MQ=255)

                                 tGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTAAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGAt                                                                                                       >  1:203908/1‑122 (MQ=255)

                                 tGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTAAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGAt                                                                                                       <  2:203908/122‑1 (MQ=255)

                                  gCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTatgat                                                                                    <  1:982657/140‑1 (MQ=255)

                                  gCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTatgat                                                                                    <  1:769700/140‑1 (MQ=255)

                                  gCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTatgat                                                                                    <  1:114590/140‑1 (MQ=255)

                                    aGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGc                                                                                  >  2:631491/1‑140 (MQ=255)

                                     gTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGc                                                                                                <  2:1237508/125‑1 (MQ=255)

                                     gTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGc                                                                                                >  1:1237508/1‑125 (MQ=255)

                                       tataACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAg                                                                               <  2:1063829/140‑1 (MQ=255)

                                               ttAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTaa                                                                       <  1:565294/140‑1 (MQ=255)

                                               ttAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTaa                                                                       <  2:1465970/140‑1 (MQ=255)

                                                tAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGAc                                                                          <  1:1350562/136‑1 (MQ=255)

                                                tAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGAc                                                                          >  2:1350562/1‑136 (MQ=255)

                                                tAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAAc                                                                      >  1:921475/1‑140 (MQ=255)

                                                 aaTGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAG‑ATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAAccc                                                                    >  2:1114224/1‑140 (MQ=255)

                                                     cTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTAt                                                                 <  1:258142/140‑1 (MQ=255)

                                                      tAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATc                                                                >  2:733463/1‑140 (MQ=255)

                                                       aGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTaa                                                                       >  2:326384/1‑132 (MQ=255)

                                                       aGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTaa                                                                       <  1:326384/132‑1 (MQ=255)

                                                       aGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCa                                                               >  1:1351789/1‑140 (MQ=255)

                                                       aGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCa                                                               >  2:1196028/1‑140 (MQ=255)

                                                             aCCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAAc                                                                      <  1:1477361/127‑1 (MQ=255)

                                                                          gTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCg                                            <  2:451193/140‑1 (MQ=255)

                                                                           tCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGc                                           <  1:665206/140‑1 (MQ=255)

                                                                           tCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGc                                           <  1:143544/140‑1 (MQ=255)

                                                                                 ttCAAGTTGTCTTAATTGCCAG‑ATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATg                                    <  1:1114224/140‑1 (MQ=255)

                                                                                  tCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATg                                    >  2:1336393/1‑140 (MQ=255)

                                                                                  tCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATg                                    <  2:921475/140‑1 (MQ=255)

                                                                                    aaGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGAc                                  <  2:1107256/140‑1 (MQ=255)

                                                                                    aaGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGAc                                  <  1:647495/140‑1 (MQ=255)

                                                                                    aaGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGAc                                  <  1:733463/140‑1 (MQ=255)

                                                                                    aaGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGAc                                  <  2:256139/140‑1 (MQ=255)

                                                                                      gTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATg                                    <  1:613654/136‑1 (MQ=255)

                                                                                      gTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATg                                    >  2:613654/1‑136 (MQ=255)

                                                                                      gTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGAccc                                >  1:1017516/1‑140 (MQ=255)

                                                                                         gTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCttt                             >  2:177451/1‑140 (MQ=255)

                                                                                         gTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCttt                             >  1:915382/1‑140 (MQ=255)

                                                                                         gTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCttt                             >  1:711904/1‑140 (MQ=255)

                                                                                         gTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCttt                             >  1:1242984/1‑140 (MQ=255)

                                                                                           cTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTa                           >  2:1188883/1‑140 (MQ=255)

                                                                                           cTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTa                           >  2:1161573/1‑140 (MQ=255)

                                                                                            ttAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAg                          >  2:217507/1‑140 (MQ=255)

                                                                                               aTTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCAc                       <  1:1336393/140‑1 (MQ=255)

                                                                                                ttGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACt                      <  2:1351789/140‑1 (MQ=255)

                                                                                                ttGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACt                      <  2:1268609/140‑1 (MQ=255)

                                                                                                ttGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACt                      <  2:711904/140‑1 (MQ=255)

                                                                                                  gCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCg                    <  1:1196028/140‑1 (MQ=255)

                                                                                                    cAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCGGt                  <  2:1017516/140‑1 (MQ=255)

                                                                                                    cAGAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCAGCAGACATGACCCTTTTAGCACTCGGt                  >  2:849106/1‑140 (MQ=255)

                                                                                                      gAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCGGTAt                <  1:1002050/140‑1 (MQ=255)

                                                                                                      gAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCGGTAt                <  1:631491/140‑1 (MQ=255)

                                                                                                      gAATCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCGGTAt                >  2:1046551/1‑140 (MQ=255)

                                                                                                       aaTCTAACGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCGGTATc               <  1:1161573/140‑1 (MQ=255)

                                                                                                             aCGGCTTTCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCGGTATCAACCAt         >  1:618942/1‑140 (MQ=255)

                                                                                                                    tCGGCAATTACTCCAAAA‑GGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCGGTATCAACCATAAAACgg  <  1:215632/140‑1 (MQ=255)

GGGGCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCGGTATCAACCATAAAACGG  >  NC_000913/1263494‑1263750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`