BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,602,017	(C)_7→8	coding (200/2019 nt)	hyfB →	hydrogenase 4 component B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,602,010	1	.	C	97.9%	324.3 / ‑0.6	95	coding (193/2019 nt)	hyfB	hydrogenase 4 component B
Reads supporting (aligned to +/- strand): ref base . (1/1); new base C (46/47); total (47/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.70e-01

Only 100 of 102 total aligned reads displayed.

TCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑G‑‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATACGAGGGCAA  >  NC_000913/2601871‑2602143

  tGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑G‑‑cc                                                                                                                                      >  2:523607/1‑140 (MQ=255)

    gCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑G‑‑cccc                                                                                                                                    <  1:1199343/140‑1 (MQ=255)

    gCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑G‑‑cccc                                                                                                                                    >  1:1158379/1‑140 (MQ=255)

                  aCTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAAttt                                                                                                                      >  1:59201/1‑140 (MQ=255)

                       gTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGa                                                                                                                 >  1:191329/1‑140 (MQ=255)

                       gTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGa                                                                                                                 >  1:738281/1‑140 (MQ=255)

                       gTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGa                                                                                                                 >  2:441357/1‑140 (MQ=255)

                       gTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGa                                                                                                                 >  1:1077985/1‑140 (MQ=255)

                       gTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGa                                                                                                                 >  2:700189/1‑140 (MQ=255)

                           ggACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTa                                                                                                             <  2:1192001/140‑1 (MQ=255)

                                 aCTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTg                                                                                                       >  1:115866/1‑140 (MQ=255)

                                 aCTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTg                                                                                                       >  2:145873/1‑140 (MQ=255)

                                 aCTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTg                                                                                                       >  1:125877/1‑140 (MQ=255)

                                 aCTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTg                                                                                                       >  1:829960/1‑140 (MQ=255)

                                 aCTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTg                                                                                                       >  1:1027682/1‑140 (MQ=255)

                                  cTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGc                                                                                                      >  2:1172133/1‑140 (MQ=255)

                                  cTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGc                                                                                                      >  1:777991/1‑140 (MQ=255)

                                  cTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑G‑‑CCCCCCACTTTTGAATTTGCCGATTTAACCCTGc                                                                                                      >  1:898738/1‑140 (MQ=255)

                                   tGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCg                                                                                                     <  2:125877/140‑1 (MQ=255)

                                    ggttATTAAGCTTCCCGGCATCACATCGCTGGGGGGCGGGGTGATTGGCATCATCAGGGGAATTCCGCAATTACTTGCTGGTTTACTTTTAGCCGCCCTTTTTCC‑‑CCCCCC‑CTTTTAATTTTGCCGATTAACCCCTGCGa                                                                                                    <  2:802686/137‑1 (MQ=255)

                                    ggCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGa                                                                                                    <  1:765407/140‑1 (MQ=255)

                                    ggCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGa                                                                                                    <  2:738281/140‑1 (MQ=255)

                                    ggCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGa                                                                                                    <  2:777991/140‑1 (MQ=255)

                                     gCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGaa                                                                                                   <  2:544712/140‑1 (MQ=255)

                                      cTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCg                                                                                                     >  1:833443/1‑137 (MQ=255)

                                      cTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCg                                                                                                     <  2:833443/137‑1 (MQ=255)

                                      cTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAAACCTGCGAAt                                                                                                  >  2:800230/1‑140 (MQ=255)

                                          tAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGAt                                                                                              <  2:191329/140‑1 (MQ=255)

                                           aaGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATa                                                                                             >  1:95093/1‑140 (MQ=255)

                                               tttCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCct                                                                                         >  1:902413/1‑140 (MQ=255)

                                               tttCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCct                                                                                         >  1:659552/1‑140 (MQ=255)

                                               tttCCGGAATCACATCGCTGGTGGGCGGGGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCct                                                                                         <  1:611362/140‑1 (MQ=255)

                                                 tCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCctct                                                                                       >  2:885462/1‑140 (MQ=255)

                                                  ccGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCtctc                                                                                      <  1:895129/140‑1 (MQ=255)

                                                  ccGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCtctc                                                                                      <  2:1027682/140‑1 (MQ=255)

                                                   cGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCg                                                                                     >  2:314851/1‑140 (MQ=255)

                                                    ggCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑G‑‑CCCCCCACTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCgg                                                                                    <  2:898738/140‑1 (MQ=255)

                                                          acaTCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTa                                                                              <  1:700189/140‑1 (MQ=255)

                                                          acaTCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTa                                                                              <  1:314851/140‑1 (MQ=255)

                                                          acaTCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTa                                                                              <  1:867938/140‑1 (MQ=255)

                                                          acaTCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTa                                                                              <  1:344526/140‑1 (MQ=255)

                                                           caTCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTAt                                                                             <  1:885462/140‑1 (MQ=255)

                                                             tCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATgg                                                                           <  1:523607/140‑1 (MQ=255)

                                                                cTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGc                                                                        >  1:581910/1‑140 (MQ=255)

                                                                cTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGc                                                                        >  1:211392/1‑140 (MQ=255)

                                                                 tggtggGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCt                                                                       <  2:211392/140‑1 (MQ=255)

                                                                   gtggGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTgg                                                                     >  2:679552/1‑140 (MQ=255)

                                                                   gtggGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTgg                                                                     >  1:1274624/1‑140 (MQ=255)

                                                                   gtggGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTgg                                                                     >  1:461338/1‑140 (MQ=255)

                                                                      ggcggcGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTg                                                                         >  2:421184/1‑133 (MQ=255)

                                                                      ggcggcGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTg                                                                         <  1:421184/133‑1 (MQ=255)

                                                                       gcggcgTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTAt                                                                 <  1:795372/140‑1 (MQ=255)

                                                                        cggcgTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTAtc                                                                >  1:160568/1‑140 (MQ=255)

                                                                        cggcgTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTAtc                                                                >  2:1079617/1‑140 (MQ=255)

                                                                          gcgTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTAtctc                                                              <  2:1158379/140‑1 (MQ=255)

                                                                                     atcatcAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCtggtgg                                                   >  1:1255557/1‑140 (MQ=255)

                                                                                        atcaGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCtggtggtgg                                                <  2:461338/140‑1 (MQ=255)

                                                                                        atcaGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCtggtggtgg                                                >  2:1475968/1‑140 (MQ=255)

                                                                                        atcaGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCtggtg                                                    <  2:812441/136‑1 (MQ=255)

                                                                                        atcaGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCtggtg                                                    >  1:812441/1‑136 (MQ=255)

                                                                                         tcaGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTggtggtggt                                               <  2:160568/140‑1 (MQ=255)

                                                                                         tcaGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTggtggtggt                                               <  2:1214153/140‑1 (MQ=255)

                                                                                             cGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGt                                           >  2:866754/1‑140 (MQ=255)

                                                                                             cGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGt                                           <  1:866754/140‑1 (MQ=255)

                                                                                             cGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGt                                           >  1:424776/1‑140 (MQ=255)

                                                                                             cGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGt                                           <  1:1172133/140‑1 (MQ=255)

                                                                                             cGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGt                                           <  2:581910/140‑1 (MQ=255)

                                                                                              ggAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGtt                                          <  2:1304018/140‑1 (MQ=255)

                                                                                              ggAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGtt                                          >  2:382566/1‑140 (MQ=255)

                                                                                               gAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTtc                                         <  1:441357/140‑1 (MQ=255)

                                                                                               gAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTtc                                         <  2:424776/140‑1 (MQ=255)

                                                                                                  ttACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTtctct                                      <  1:679552/140‑1 (MQ=255)

                                                                                                  ttACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTtctct                                      <  2:659552/140‑1 (MQ=255)

                                                                                                    aCGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTctctct                                    <  1:1079617/140‑1 (MQ=255)

                                                                                                     cGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GCCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTctctct                                    >  1:717915/1‑140 (MQ=255)

                                                                                                     cGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTa                                   >  1:1236999/1‑140 (MQ=255)

                                                                                                      gCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTAt                                  <  2:897643/140‑1 (MQ=255)

                                                                                                       cAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GCCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTa                                   >  1:549634/1‑139 (MQ=255)

                                                                                                       cAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GCCCCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTa                                   <  2:549634/139‑1 (MQ=255)

                                                                                                        aaTTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTc                                <  2:1077985/140‑1 (MQ=255)

                                                                                                          ttACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTTGTGGTTTGTTCTCTCTATTCAt                              <  2:829960/140‑1 (MQ=255)

                                                                                                          ttACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCAt                              >  2:1011711/1‑140 (MQ=255)

                                                                                                          ttACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCAt                              >  2:389763/1‑140 (MQ=255)

                                                                                                          ttACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCAt                              >  1:1194351/1‑140 (MQ=255)

                                                                                                                   gttgtAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTtata                     <  1:800230/138‑1 (MQ=255)

                                                                                                                    gtgtAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCAt                              >  1:1061835/1‑130 (MQ=255)

                                                                                                                    gtgtAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCAt                              <  2:1061835/130‑1 (MQ=255)

                                                                                                                    gtgtAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTatat                    >  1:1299264/1‑140 (MQ=255)

                                                                                                                    gtgtAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTatat                    >  2:1376125/1‑140 (MQ=255)

                                                                                                                    gtgtAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTatat                    <  1:389763/140‑1 (MQ=255)

                                                                                                                    gtgtAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTatat                    <  1:1011711/140‑1 (MQ=255)

                                                                                                                    gtgtAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTACTGGTTATCTCCTTGCTGGTGGTGGTTTGTtctctc                                     <  2:649062/123‑1 (MQ=255)

                                                                                                                    gtgtAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTACTGGTTATCTCCTTGCTGGTGGTGGTTTGTtctctc                                     >  1:649062/1‑123 (MQ=255)

                                                                                                                     tgtAACTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATg                   <  1:1376125/140‑1 (MQ=255)

                                                                                                                        aaCTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATgcgc                <  1:1475968/140‑1 (MQ=255)

                                                                                                                          cTTTAGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGa              >  1:1380799/1‑140 (MQ=255)

                                                                                                                              aGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATAc          <  2:1255557/140‑1 (MQ=255)

                                                                                                                              aGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATAc          >  1:975253/1‑140 (MQ=255)

                                                                                                                              aGTCGCCCGTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGCTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATAc          <  2:1299264/140‑1 (MQ=255)

                                                                                                                                      gTTTT‑GC‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATACGAGGGCaa  <  2:1236999/140‑1 (MQ=255)

TCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑G‑‑CCCCCC‑CTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATACGAGGGCAA  >  NC_000913/2601871‑2602143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`