BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,981,901	(C)_5→6	coding (282/1419 nt)	araE ←	arabinose:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,981,896	1	.	C	98.4%	412.9 / ‑3.7	124	A96G (GCC→GGC)	araE	arabinose:H(+) symporter
Reads supporting (aligned to +/- strand): ref base . (0/2); major base C (64/56); minor base T (1/1); total (65/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 128 total aligned reads displayed.

GCCATTTCAGAAAGATACAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGG‑CCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCG  >  NC_000913/2981761‑2982030

gCCATTTCAGAAAGATACAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGG‑cccc                                                                                                                                     >  1:461965/1‑140 (MQ=255)

 ccATTTCAGAAAGATACAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGG‑cccc‑c                                                                                                                                   <  2:405849/140‑1 (MQ=255)

     ttCAGAAAGATACAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGG‑cccc‑ccgcc                                                                                                                               >  2:472963/1‑136 (MQ=255)

      tCAGAAAGATACAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGG‑cccc‑ccgcca                                                                                                                              <  2:461965/140‑6 (MQ=255)

       cAGAAAGATACAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCAt                                                                                                                              <  1:1352364/140‑1 (MQ=255)

          aaaGATACAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAg                                                                                                                           <  1:593932/140‑1 (MQ=255)

              aTACAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGG‑CCCCNCGCCATCAGGCTg                                                                                                                       <  1:1371680/140‑1 (MQ=255)

               tACAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGt                                                                                                                      >  2:1117886/1‑140 (MQ=255)

                     ggagCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTAc                                                                                                                <  1:1117886/140‑1 (MQ=255)

                       agCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGc                                                                                                              >  2:243039/1‑140 (MQ=255)

                        gCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGcc                                                                                                             >  1:595478/1‑140 (MQ=255)

                        gCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGcc                                                                                                             >  1:235463/1‑140 (MQ=255)

                        gCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGcc                                                                                                             >  2:1163525/1‑140 (MQ=255)

                        gCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGcc                                                                                                             >  1:94134/1‑140 (MQ=255)

                         cGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCAt                                                                                                                              <  1:1376815/122‑1 (MQ=255)

                         cGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCAt                                                                                                                              >  2:1376815/1‑122 (MQ=255)

                         cGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATc                                                                                                                             <  2:388863/123‑1 (MQ=255)

                         cGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATc                                                                                                                             >  1:388863/1‑123 (MQ=255)

                         cGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGccc                                                                                                            >  2:1276079/1‑140 (MQ=255)

                         cGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGccc                                                                                                            >  1:691627/1‑140 (MQ=255)

                              tAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGc                                                                                                       >  1:92927/1‑140 (MQ=255)

                              tAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGc                                                                                                       >  1:611892/1‑140 (MQ=255)

                                ataCGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCgg                                                                                                     <  2:1171130/138‑1 (MQ=255)

                                aGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCgg                                                                                                     >  1:182444/1‑140 (MQ=255)

                                     cgATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGa                                                                                                >  2:490050/1‑140 (MQ=255)

                                      gATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGAc                                                                                               >  1:1092090/1‑140 (MQ=255)

                                       aTCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGaa                                                                                                   <  2:526049/135‑1 (MQ=255)

                                        tCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAg                                                                                             >  2:665134/1‑140 (MQ=255)

                                        tCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAg                                                                                             >  2:709356/1‑140 (MQ=255)

                                          ccGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATTGC‑CCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGcc                                                                                           <  2:455875/140‑1 (MQ=255)

                                          ccGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGcc                                                                                           <  1:709356/140‑1 (MQ=255)

                                          ccGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGcc                                                                                           <  1:979566/140‑1 (MQ=255)

                                          ccGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGcc                                                                                           <  2:1033187/140‑1 (MQ=255)

                                          ccGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGcc                                                                                           <  2:1092090/140‑1 (MQ=255)

                                          ccGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGcc                                                                                           <  1:243039/140‑1 (MQ=255)

                                          ccGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGcc                                                                                           <  1:1276079/140‑1 (MQ=255)

                                          ccGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACACCCCAGGCGGAACGACAGcc                                                                                           <  2:1344543/140‑1 (MQ=255)

                                            gACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCaa                                                                                         <  2:42419/140‑1 (MQ=255)

                                            gACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCaa                                                                                         <  2:1251583/140‑1 (MQ=255)

                                             aCAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAAc                                                                                        <  2:235463/140‑1 (MQ=255)

                                                  aaTGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGTCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTa                                                                                   >  1:1459038/1‑140 (MQ=255)

                                                    tGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTaaa                                                                                 >  2:1076509/1‑140 (MQ=255)

                                                    tGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTaaa                                                                                 >  2:359652/1‑140 (MQ=255)

                                                      cccAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACa                                                                               >  2:851124/1‑140 (MQ=255)

                                                        cAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAgc                                                                             <  1:339413/140‑1 (MQ=255)

                                                          gCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGcc                                                                                           >  1:1423880/1‑124 (MQ=255)

                                                             ccacACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCAcc                                                                        <  1:981979/140‑1 (MQ=255)

                                                                cacGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAg                                                                              <  2:1171807/131‑1 (MQ=255)

                                                                cacGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAg                                                                              >  1:1171807/1‑131 (MQ=255)

                                                                cacGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAAt                                                                     >  2:1391026/1‑140 (MQ=255)

                                                                cacGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAAt                                                                     >  2:845669/1‑140 (MQ=255)

                                                                    aGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGTCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATtgct                                                                 <  2:1459038/140‑1 (MQ=255)

                                                                    aGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATtgct                                                                 <  1:1076509/140‑1 (MQ=255)

                                                                    aGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATtgct                                                                 <  1:880641/140‑1 (MQ=255)

                                                                            ttAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAg                                                         <  1:359652/140‑1 (MQ=255)

                                                                            ttAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAg                                                         <  1:200665/140‑1 (MQ=255)

                                                                            ttAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAg                                                         <  2:611892/140‑1 (MQ=255)

                                                                            ttAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAg                                                         <  2:92927/140‑1 (MQ=255)

                                                                            ttAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATCAAACAGCGCACCAATTGCTGCACCGAg                                                         <  2:1389713/140‑1 (MQ=255)

                                                                                   ctctACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATg                                                  >  2:658525/1‑140 (MQ=255)

                                                                                     ctACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGct                                                <  1:1391026/140‑1 (MQ=255)

                                                                                        cGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGATAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGcatca                                                    >  2:86934/1‑133 (MQ=255)

                                                                                        cGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGATAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGcatca                                                    <  1:86934/133‑1 (MQ=255)

                                                                                          cTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTaa                                           >  1:306471/1‑140 (MQ=255)

                                                                                            ggTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAacc                                         <  2:182444/140‑1 (MQ=255)

                                                                                             gTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAg                                                         <  1:195888/123‑1 (MQ=255)

                                                                                             gTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGcat                                                      >  1:649693/1‑126 (MQ=255)

                                                                                             gTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGct                                                <  1:809013/132‑1 (MQ=255)

                                                                                             gTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAacca                                        >  2:697310/1‑140 (MQ=255)

                                                                                             gTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAacca                                        >  2:948270/1‑140 (MQ=255)

                                                                                             gTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAacca                                        >  2:617549/1‑140 (MQ=255)

                                                                                             gTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAacca                                        >  2:544/1‑140 (MQ=255)

                                                                                             gTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAacca                                        >  1:886853/1‑140 (MQ=255)

                                                                                             gTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAacca                                        >  1:366973/1‑140 (MQ=255)

                                                                                             gTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAacca                                        >  1:972161/1‑140 (MQ=255)

                                                                                                  aaaaGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCAt                                   <  2:972161/140‑1 (MQ=255)

                                                                                                    aaGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTc                                 <  1:855944/140‑1 (MQ=255)

                                                                                                              tataGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGa                       <  1:658525/140‑1 (MQ=255)

                                                                                                              tataGAACCGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGa                       <  2:1161459/140‑1 (MQ=255)

                                                                                                                     ccGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCa                <  1:1020831/140‑1 (MQ=255)

                                                                                                                      cGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGa                       <  1:1293532/132‑1 (MQ=255)

                                                                                                                      cGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTgg                   >  1:563635/1‑136 (MQ=255)

                                                                                                                      cGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTgg                   <  2:563635/136‑1 (MQ=255)

                                                                                                                      cGAGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAg               >  1:163864/1‑140 (MQ=255)

                                                                                                                        aGTACGAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGca             >  2:1239524/1‑140 (MQ=255)

                                                                                                                        aGTACAAACAGGA‑TGGCCCCC‑CGCCGTCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGca             >  1:1170283/1‑140 (MQ=255)

                                                                                                                        aGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGa                       >  1:636496/1‑130 (MQ=255)

                                                                                                                        aGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGa                       <  2:636496/130‑1 (MQ=255)

                                                                                                                        aGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGt                  >  2:844164/1‑135 (MQ=255)

                                                                                                                        aGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGca             >  1:238766/1‑140 (MQ=255)

                                                                                                                        aGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGca             >  1:468089/1‑140 (MQ=255)

                                                                                                                        aGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGca             >  1:449115/1‑140 (MQ=255)

                                                                                                                        aGTACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGca             >  2:650012/1‑140 (MQ=255)

                                                                                                                          tACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGcaca           >  2:665702/1‑140 (MQ=255)

                                                                                                                          tACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGcaca           >  2:193402/1‑140 (MQ=255)

                                                                                                                          tACAAACAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGcaca           <  2:306471/140‑1 (MQ=255)

                                                                                                                               aCAGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTg      <  1:85801/140‑1 (MQ=255)

                                                                                                                                 aGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGAt    <  2:691627/140‑1 (MQ=255)

                                                                                                                                 aGGA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGAt    <  2:238766/140‑1 (MQ=255)

                                                                                                                                   gA‑TGGCCCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCg  >  1:696775/1‑140 (MQ=255)

GCCATTTCAGAAAGATACAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGA‑TGG‑CCCC‑CGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCACAAAGTGATCG  >  NC_000913/2981761‑2982030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`