Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,332,680	(C)6→7	coding (56/966 nt)	yhbE ←	inner membrane protein YhbE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,332,674	1	.	C	100.0%	187.9 / NA	54	A21G (GCG→GGG)	yhbE	inner membrane protein YhbE
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (23/31);  total (23/31)

CGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACG‑‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTA  >  NC_000913/3332543‑3332798                                                                                                                                     |                                                                                                                              cgcgTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACG‑‑ccccccca                                                                                                                      <  1:868788/140‑3 (MQ=255)   cgTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACG‑‑cccccccagc                                                                                                                    >  2:474188/1‑136 (MQ=255)    gTAATGGCGGCAACCTCTTCTTCACCCCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACG‑‑cccccccagca                                                                                                                   <  1:98854/140‑6 (MQ=255)                           aCCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCaaaa                                                                                             >  1:625751/1‑140 (MQ=255)                              gCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGaa                                                                                          >  2:502414/1‑140 (MQ=255)                               cAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGaat                                                                                         >  1:757230/1‑140 (MQ=255)                                          cccAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAAtgcctg                                                                              >  2:23566/1‑140 (MQ=255)                                              ggCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGcctgcctg                                                                          <  2:481353/140‑1 (MQ=255)                                              ggCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGcctgcctg                                                                          <  1:502414/140‑1 (MQ=255)                                              ggCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGcctgcctg                                                                          >  2:350868/1‑140 (MQ=255)                                               gCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCctgcctgc                                                                         >  2:527567/1‑140 (MQ=255)                                                   aTACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTgctgct                                                                     <  1:851741/140‑1 (MQ=255)                                                    tACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCtt                                                                    >  1:9513/1‑140 (MQ=255)                                                     aCTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTc                                                                   <  1:1153137/140‑1 (MQ=255)                                                      cTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCa                                                                  <  2:1104231/140‑1 (MQ=255)                                                      cTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCa                                                                  <  2:148724/140‑1 (MQ=255)                                                       tCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCAt                                                                 <  1:23566/140‑1 (MQ=255)                                                       tCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCAt                                                                 <  1:474188/140‑1 (MQ=255)                                                       tCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCAt                                                                 <  1:527567/140‑1 (MQ=255)                                                       tCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCAt                                                                 <  2:757230/140‑1 (MQ=255)                                                         gCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTgctgct                                                                     <  1:332500/134‑1 (MQ=255)                                                         gCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTgctgct                                                                     >  2:332500/1‑134 (MQ=255)                                                         gCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTc                                                                   <  1:101464/136‑1 (MQ=255)                                                         gCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTc                                                                   >  2:101464/1‑136 (MQ=255)                                                         gCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATAc                                                               >  1:1395778/1‑140 (MQ=255)                                                         gCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATAc                                                               >  2:1162264/1‑140 (MQ=255)                                                                    aCGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTg                                                    >  1:127204/1‑140 (MQ=255)                                                                          aaaCACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAg                                               <  2:303733/140‑1 (MQ=255)                                                                          aaaCACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAgc                                              <  1:350868/140‑1 (MQ=255)                                                                           aaCACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAgcg                                             <  2:1275242/140‑1 (MQ=255)                                                                            acacGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAgcgc                                            <  2:1322487/140‑1 (MQ=255)                                                                                 aTTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTg                                       >  2:391923/1‑140 (MQ=255)                                                                                 aTTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTg                                       <  2:625751/140‑1 (MQ=255)                                                                                     tCGGAGGTTCCATCACCTCCTGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGa                                   >  2:604120/1‑140 (MQ=255)                                                                                      cGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGAt                                  <  2:522199/140‑1 (MQ=255)                                                                                        gAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTa                                <  2:1395778/140‑1 (MQ=255)                                                                                          ggTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAAc                              >  1:717491/1‑140 (MQ=255)                                                                                            ttCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACg                             >  2:104599/1‑140 (MQ=255)                                                                                               cATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGAt                                  <  2:1247428/131‑1 (MQ=255)                                                                                               cATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGAt                                  >  1:1247428/1‑131 (MQ=255)                                                                                               cATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGc                         >  1:654558/1‑140 (MQ=255)                                                                                                 tCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCAc                       <  2:1260251/140‑1 (MQ=255)                                                                                                  cACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCAcc                      <  2:999248/140‑1 (MQ=255)                                                                                                     cTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATg                   >  2:1203827/1‑140 (MQ=255)                                                                                                     cTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATg                   <  2:654558/140‑1 (MQ=255)                                                                                                      tCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATgc                  <  1:1162264/140‑1 (MQ=255)                                                                                                       cctgCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATgcg                 <  1:604120/137‑1 (MQ=255)                                                                                                           cACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATg                   <  2:371862/134‑1 (MQ=255)                                                                                                           cACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATg                   >  1:371862/1‑134 (MQ=255)                                                                                                           cACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCAtt             >  2:1205489/1‑140 (MQ=255)                                                                                                               tGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTa         >  2:75898/1‑140 (MQ=255)                                                                                                                gCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTAc        <  2:127204/140‑1 (MQ=255)                                                                                                                gCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTAc        <  1:317535/140‑1 (MQ=255)                                                                                                                gCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTAc        <  1:1382416/140‑1 (MQ=255)                                                                                                                gCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTAc        <  1:1205489/140‑1 (MQ=255)                                                                                                                gCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCACATTTTTAc        <  1:10162/140‑1 (MQ=255)                                                                                                                      ttGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAACGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTa  >  1:1046020/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                              CGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACG‑‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTA  >  NC_000913/3332543‑3332798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑