Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,459,316 (A)7→8 intergenic (+5/+584) pmbA → / ← nrdG metalloprotease PmbA/anaerobic ribonucleoside‑triphosphate reductase activating protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,459,3091.A98.9% 333.5 / ‑3.9 94coding (1351/1353 nt)pmbAmetalloprotease PmbA
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base A (51/42);  total (52/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

Only 100 of 101 total aligned reads displayed.

GGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGT‑AAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAGTC  >  NC_000913/4459173‑4459443
                                                                                                                                         |                                                                                                                                      
ggTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGT‑aaa                                                                                                                                     >  2:296705/1‑140 (MQ=255)
  tGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGT‑aaaaa                                                                                                                                   >  1:684987/1‑140 (MQ=255)
    aGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGT‑aaaaaaa                                                                                                                                 >  1:330670/1‑140 (MQ=255)
     gCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGT‑aaaaaaaa                                                                                                                                <  1:296705/140‑2 (MQ=255)
        aaaTCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGT‑aaaaaaaactg                                                                                                                             <  2:330670/140‑5 (MQ=255)
        aaaTCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGT‑aaaaaaaactg                                                                                                                             >  1:452281/1‑136 (MQ=255)
        aaaTCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGT‑aaaaaaaactg                                                                                                                             <  2:1212250/140‑5 (MQ=255)
                aTCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGAcc                                                                                                                      <  1:694554/140‑1 (MQ=255)
                   gCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGc                                                                                                                   >  2:1253555/1‑140 (MQ=255)
                    cAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGcc                                                                                                                  <  2:272301/140‑1 (MQ=255)
                    cAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGcc                                                                                                                  <  2:635867/140‑1 (MQ=255)
                    cAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGcc                                                                                                                  <  2:1184358/140‑1 (MQ=255)
                      ggTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCcgc                                                                                                                <  1:1345133/140‑1 (MQ=255)
                                           cgcAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTaca                                                                                           <  2:209606/140‑1 (MQ=255)
                                               atatTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACaaa                                                                                       <  2:452281/140‑1 (MQ=255)
                                                       aCCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCg                                                                               >  1:954098/1‑140 (MQ=255)
                                                          gTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCAc                                                                            >  1:1296118/1‑140 (MQ=255)
                                                          gTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCAc                                                                            >  1:348010/1‑140 (MQ=255)
                                                           tCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACt                                                                           >  2:501914/1‑140 (MQ=255)
                                                           tCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGT‑AAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTg                                                                          >  1:1201493/1‑140 (MQ=255)
                                                            cGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTg                                                                          >  1:793200/1‑140 (MQ=255)
                                                            cGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTg                                                                          >  2:219791/1‑140 (MQ=255)
                                                            cGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTg                                                                          >  1:625960/1‑140 (MQ=255)
                                                            cGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAAGTACACAAAATCATTCGCACTg                                                                          >  2:1344941/1‑140 (MQ=255)
                                                             ggTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGc                                                                         >  1:1420662/1‑140 (MQ=255)
                                                                aaCGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATc                                                                      <  2:1296118/140‑1 (MQ=255)
                                                                    atatTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCgaga                                                                  <  2:449751/140‑1 (MQ=255)
                                                                      atTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACg                                                                >  1:552199/1‑140 (MQ=255)
                                                                             cacGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGa                                                              <  2:136814/135‑1 (MQ=255)
                                                                             cacGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGa                                                              >  1:136814/1‑135 (MQ=255)
                                                                               cGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGaa                                                       >  1:165899/1‑140 (MQ=255)
                                                                               cGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGaa                                                       >  2:326693/1‑140 (MQ=255)
                                                                               cGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGaa                                                       >  1:320836/1‑140 (MQ=255)
                                                                                caggTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAAt                                                      <  1:839427/137‑1 (MQ=255)
                                                                                 cAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATg                                                     >  1:705013/1‑140 (MQ=255)
                                                                                        ataCAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCg                                              <  1:508396/140‑1 (MQ=255)
                                                                                              tgtgGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAAt                                                      >  1:255694/1‑126 (MQ=255)
                                                                                              tgtgGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAAt                                                      <  2:255694/126‑1 (MQ=255)
                                                                                              tgtgGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGcc                                               <  2:528992/133‑1 (MQ=255)
                                                                                              tgtgGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGcc                                               >  1:528992/1‑133 (MQ=255)
                                                                                              tgtgGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCgggg                                           >  1:297955/1‑137 (MQ=255)
                                                                                              tgtgGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCgggg                                           <  2:297955/137‑1 (MQ=255)
                                                                                              tgtgGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCgggg                                           <  1:150193/137‑1 (MQ=255)
                                                                                              tgtgGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCgggg                                           <  2:978145/137‑1 (MQ=255)
                                                                                              tgtgGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCgggg                                           >  1:978145/1‑137 (MQ=255)
                                                                                              tgtgGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCgggg                                           >  2:150193/1‑137 (MQ=255)
                                                                                              tgtgGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAg                                         >  1:1262324/1‑139 (MQ=255)
                                                                                              tgtgGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAg                                         <  2:1262324/139‑1 (MQ=255)
                                                                                              tgtgGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGc                                        >  1:1040595/1‑140 (MQ=255)
                                                                                              tgtgGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGc                                        >  2:1374549/1‑140 (MQ=255)
                                                                                              tgtgGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGc                                        >  2:240777/1‑140 (MQ=255)
                                                                                              tgtgGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGc                                        >  2:126096/1‑140 (MQ=255)
                                                                                              tgtgGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGc                                        >  2:1062239/1‑140 (MQ=255)
                                                                                              tgtgGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGc                                        >  1:97303/1‑140 (MQ=255)
                                                                                              tgtgGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGc                                        >  1:619630/1‑140 (MQ=255)
                                                                                                tgGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCggg                                            <  1:20762/134‑1 (MQ=255)
                                                                                                tgGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCggg                                            >  2:20762/1‑134 (MQ=255)
                                                                                                tgGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGt                                      >  2:1220957/1‑140 (MQ=255)
                                                                                                tgGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGt                                      >  2:949941/1‑140 (MQ=255)
                                                                                                tgGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGt                                      >  1:1469368/1‑140 (MQ=255)
                                                                                                tgGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGt                                      >  1:340435/1‑140 (MQ=255)
                                                                                                tgGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGt                                      >  2:22973/1‑140 (MQ=255)
                                                                                                tgGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGt                                      >  1:113045/1‑140 (MQ=255)
                                                                                                 ggTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTa                                     <  2:793200/140‑1 (MQ=255)
                                                                                                 ggTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTa                                     >  2:315139/1‑140 (MQ=255)
                                                                                                 ggTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTa                                     <  2:320836/140‑1 (MQ=255)
                                                                                                   ttCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACt                                   <  2:625960/140‑1 (MQ=255)
                                                                                                   ttCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACt                                   <  1:1062239/140‑1 (MQ=255)
                                                                                                   ttCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACt                                   >  2:135847/1‑140 (MQ=255)
                                                                                                      tgtgCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGc                                <  1:949941/140‑1 (MQ=255)
                                                                                                       gtgCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCa                               <  1:219791/140‑1 (MQ=255)
                                                                                                        tgCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCgg                                             >  2:722651/1‑125 (MQ=255)
                                                                                                        tgCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCgg                                             <  1:722651/125‑1 (MQ=255)
                                                                                                        tgCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCgg                                             <  1:773569/125‑1 (MQ=255)
                                                                                                        tgCTGTTGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCgg                                             >  2:773569/1‑125 (MQ=255)
                                                                                                             ttGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAg                                         <  2:718427/124‑1 (MQ=255)
                                                                                                             ttGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAg                                         >  1:718427/1‑124 (MQ=255)
                                                                                                             ttGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCg                                       <  2:991701/126‑1 (MQ=255)
                                                                                                             ttGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCg                                       >  1:991701/1‑126 (MQ=255)
                                                                                                             ttGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGt                         >  2:959813/1‑140 (MQ=255)
                                                                                                             ttGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGt                         >  2:187039/1‑140 (MQ=255)
                                                                                                              tGCCGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTg                        <  1:326693/140‑1 (MQ=255)
                                                                                                                ccGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGAc                      <  2:348010/140‑1 (MQ=255)
                                                                                                                ccGGAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGAc                      >  2:1434774/1‑140 (MQ=255)
                                                                                                                  ggAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGAccc                    >  1:1267171/1‑140 (MQ=255)
                                                                                                                  ggAGATGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGAccc                    >  2:656911/1‑140 (MQ=255)
                                                                                                                    agaTGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCgg                  <  2:619630/140‑1 (MQ=255)
                                                                                                                       tGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGc                                <  1:724400/123‑1 (MQ=255)
                                                                                                                       tGAAAATCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGc                                >  2:724400/1‑123 (MQ=255)
                                                                                                                          aaaTCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAAt            <  2:552199/140‑1 (MQ=255)
                                                                                                                          aaaTCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAAt            <  2:1267171/140‑1 (MQ=255)
                                                                                                                          aaaTCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAAt            <  2:1420662/140‑1 (MQ=255)
                                                                                                                            aTCGCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGa          <  2:705013/140‑1 (MQ=255)
                                                                                                                               gCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCg       <  1:656911/140‑1 (MQ=255)
                                                                                                                               gCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCg       <  2:113045/140‑1 (MQ=255)
                                                                                                                               gCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCg       <  1:187039/140‑1 (MQ=255)
                                                                                                                               gCCGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCg       <  2:165899/140‑1 (MQ=255)
                                                                                                                                ccGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGt      >  1:39606/1‑140 (MQ=255)
                                                                                                                                ccGGACAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGt      >  2:924342/1‑140 (MQ=255)
                                                                                                                                    aCAGTAAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAgtc  <  1:790152/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                      
GGTGAGCGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAACGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGACAGT‑AAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAGTC  >  NC_000913/4459173‑4459443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑