Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,510,788	(A)8→9	coding (786/1407 nt)	ydcR →	fused putative DNA‑binding transcriptional regulator/putative aminotransferase YdcR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,510,780	1	.	A	100.0%	152.8 / NA	44	Q260K (CAA→AAA)	ydcR	fused putative DNA‑binding transcriptional regulator/putative aminotransferase YdcR
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (20/24);  total (20/24)

AAGGCGTTATCGGTGGCGACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGC‑AAAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAGATGACGTTTACAGCGAACTTTATTTTGGACGGGAAAAACCGCTGCCTGCGAAAGCGTGGGATCGCCACGATGGCGTT  >  NC_000913/1510642‑1510914                                                                                                                                            |                                                                                                                                       aaGGCGTTATCGGTGGCGACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGC‑a                                                                                                                                       >  1:618812/1‑140 (MQ=255) aaGGCGTTATCGGTGGCGACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGC‑a                                                                                                                                       >  1:879289/1‑140 (MQ=255)  aGGCGTTATCGGTGGCGACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGC‑aa                                                                                                                                      >  1:675670/1‑140 (MQ=255)            ggTGGCGACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGC‑aaaaaaaaagca                                                                                                                            <  2:618812/140‑5 (MQ=255)             gTGGCGACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGC‑aaaaaaaaagcac                                                                                                                           <  2:879289/140‑6 (MQ=255)             gTGGCGACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGC‑aaaaaaaaagcac                                                                                                                           <  2:766964/140‑6 (MQ=255)              tGGCGACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAAGcaca                                                                                                                           >  2:563686/1‑140 (MQ=255)                   aCGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGC‑aaaaaaaaagcac                                                                                                                           <  1:265926/134‑6 (MQ=255)                   aCGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGC‑aaaaaaaaagcac                                                                                                                           >  2:265926/1‑129 (MQ=255)                       aTGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAAGCACAACTGGTGGc                                                                                                                  <  2:242885/140‑1 (MQ=255)                       aTGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAAGCACAACTGGTGGc                                                                                                                  <  1:1281980/140‑1 (MQ=255)                            aaaGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAAGCACAACTGGTGGCGTTGc                                                                                                             <  1:1471773/140‑1 (MQ=255)                            aaaGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAAGCACAACTGGTGGCGTTGc                                                                                                             <  2:1172165/140‑1 (MQ=255)                               gaagGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAAGCACAACTGGTGGCGTTGc                                                                                                             <  2:1379339/137‑1 (MQ=255)                               gaagGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAAGCACAACTGGTGGCGTTGc                                                                                                             >  1:248703/1‑137 (MQ=255)                               gaagGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAAGCACAACTGGTGGCGTTGc                                                                                                             >  1:1379339/1‑137 (MQ=255)                               gaagGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAAGCACAACTGGTGGCGTTGc                                                                                                             <  2:248703/137‑1 (MQ=255)                                 agGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAAGCACAACtgg                                                                                                                      <  2:1110929/126‑1 (MQ=255)                                 agGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAAGCACAACtgg                                                                                                                      >  1:1110929/1‑126 (MQ=255)                                 agGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAAGCACAACTGGTGGCGTTGCTCAAt                                                                                                        <  1:72327/140‑1 (MQ=255)                                   ggATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAAGCACAACTGGTGGCGTTGCTCAATCa                                                                                                      >  1:828825/1‑140 (MQ=255)                                    gATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAAGCACAACTGGTGGCGTTGCTCAATCAg                                                                                                     >  2:1195202/1‑140 (MQ=255)                                        gATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAAGCACAACTGGTGGCGTTGCTCAAt                                                                                                        <  1:481915/133‑1 (MQ=255)                                        gATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAAGCACAACTGGTGGCGTTGCTCAAt                                                                                                        >  2:481915/1‑133 (MQ=255)                                                  cgcTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCt                                                                                       <  2:675670/140‑1 (MQ=255)                                                   gcTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACg                                                                                         >  2:203443/1‑137 (MQ=255)                                                   gcTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACg                                                                                         <  1:203443/137‑1 (MQ=255)                                                       gAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTg                                                                                  <  1:1195202/140‑1 (MQ=255)                                                          cTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAg                                                                               <  2:764421/140‑1 (MQ=255)                                                             gCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAGATg                                                                            >  1:1401345/1‑140 (MQ=255)                                                                      gAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAGATGACGTTTACa                                                                   <  1:563686/140‑1 (MQ=255)                                                                        gTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAGATGACGTTTACAGc                                                                 >  2:73581/1‑140 (MQ=255)                                                                        gTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAGATGACGTTTACAGc                                                                 >  2:336794/1‑140 (MQ=255)                                                                           tCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAGATGACGTTTACAGCGaa                                                              >  2:148878/1‑140 (MQ=255)                                                                           tCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAGATGACGTTTACAGCGaa                                                              >  1:808841/1‑140 (MQ=255)                                                                            ccGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAGTCCACTCGGATTTACCTTAACGCCGCAAAAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGAAACGCTGATTGAAGATGACGTTTACATCGAAc                                                             >  2:1305824/1‑140 (MQ=255)                                                                                tGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAGATGACGTTTACAGCGAACt                                                            <  1:88989/137‑1 (MQ=255)                                                                                tGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAGATGACGTTTACAGCGAACt                                                            >  2:88989/1‑137 (MQ=255)                                                                                   aaGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAGATGACGTTTACAGCGAACTTTAttt                                                      >  2:189419/1‑140 (MQ=255)                                                                                   aaGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAGATGACGTTTACAGCGAACTTTAttt                                                      >  1:956859/1‑140 (MQ=255)                                                                                                   gaCTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAGATGACGTTTACAGCGAACTTTATTTTGGACGGGAAAAACCg                                      <  2:520891/140‑1 (MQ=255)                                                                                                   gaCTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAGATGACGTTTACAGCGAACTTTATTTTGGACGGGAAAAACCg                                      <  2:520892/140‑1 (MQ=255)                                                                                                   gaCTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAGATGACGTTTACAGCGAACTTTATTTTGGACGGGAAAAACCg                                      <  2:1411315/140‑1 (MQ=255)                                                                                                       aaTAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAGATGACGTTTACAGCGAACTTTATTTTGGACGGGAAAAACCGctgc                                  <  2:828825/140‑1 (MQ=255)                                                                                                        aTAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAGATGACGTTTACAGCGAACTTTATTTTGGACGGGAAAAACCg                                      <  1:871254/135‑1 (MQ=255)                                                                                                        aTAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAGATGACGTTTACAGCGAACTTTATTTTGGACGGGAAAAACCg                                      >  2:871254/1‑135 (MQ=255)                                                                                                             cAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAGATGACGTTTACAGCGAACTTTATTTTGGACGGGAAAAACCGCTGCCTGCGa                            <  2:956859/140‑1 (MQ=255)                                                                                                                      cTCGGATTTACCTTAACGCCGCAAAAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAGATGACGTTTACAGCGAACTTTATTTTGGACGGGAAAAACCGCTGCCTGCGAAAGCGTggg                   >  1:161837/1‑140 (MQ=255)                                                                                                                            tttACCTTAACGCCGCAAAAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAGATGACGTTTACAGCGAACTTTATTTTGGACGGGAAAAACCGCTGCCTGCGAAAGCGTGGGATCGcc             >  1:152260/1‑140 (MQ=255)                                                                                                                                  ttAACGCCGCAAAAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAGATGACGTTTACAGCGAACTTTATTTTGGACGGGAAAAACCGCTGCCTGCGAAAGCGTGGGATCGCCACGATg       <  1:66526/140‑1 (MQ=255)                                                                                                                                  ttAACGCCGCAAAAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAGATGACGTTTACAGCGAACTTTATTTTGGACGGGAAAAACCGCTGCCTGCGAAAGCGTGGGATCGCCACGATg       <  2:161837/140‑1 (MQ=255)                                                                                                                                  ttAACGCCGCAAAAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAGATGACGTTTACAGCGAACTTTATTTTGGACGGGAAAAACCGCTGCCTGCGAAAGCGTGGGATCGCCACGATg       <  1:1305824/140‑1 (MQ=255)                                                                                                                                     aCGCCGCAAAAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAGATGACGTTTACAGCGAACTTTATTTTGGACGGGAAAAACCGCTGCCTGCGAAAGCGTGGGATCGCCACGATGGCg    >  1:137065/1‑140 (MQ=255)                                                                                                                                       gccgcaAAAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAGATGACGTTTACAGCGAACTTTATTTTGGACGGGAAAAACCGCTGCCTGCGAAAGCGTGGGATCGCCACGATGGCGtt  <  1:25937/140‑1 (MQ=255)                                                                                                                                            |                                                                                                                                       AAGGCGTTATCGGTGGCGACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGC‑AAAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAGATGACGTTTACAGCGAACTTTATTTTGGACGGGAAAAACCGCTGCCTGCGAAAGCGTGGGATCGCCACGATGGCGTT  >  NC_000913/1510642‑1510914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑