Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,925,000	(T)6→7	intergenic (‑31/‑108)	yobA ← / → holE	CopC domain‑containing protein/DNA polymerase III subunit theta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,924,994	1	.	T	100.0%	274.8 / NA	77	intergenic (‑25/‑114)	yobA/holE	CopC domain‑containing protein/DNA polymerase III subunit theta
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (40/37);  total (40/37)

CGTTTGCCGCAGGATACTGATGCGTTAAATGCGCATGTGCCCAGACGGAAGGTGTCACCAACGAAGTGGTCAGGATTGCCAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAAC‑TTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTCCGGCTTGCCATCCTGGCTCACTCTTAGTAACTTTTGCCCGCGAATGATGAGGAGATTAAGAATGCTGAAGAATCTGGCTAAA  >  NC_000913/1924855‑1925128                                                                                                                                             |                                                                                                                                       cgTTTGCCGCAGGATACTGATGCGTTAAATGCGCATGTGCCCAGACGGAAGGTGTCACCAACGAAGTGGTCAGGATTGCCAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAAc                                                                                                                                         <  2:1317901/140‑1 (MQ=255)         gCAGGATACTGATGCGTTAAATGCGCATGTGCCCAGACGGAAGGTGTCACCAACGAAGTGGTCAGGATTGCCAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAAC‑ttttttta                                                                                                                                <  2:948284/140‑3 (MQ=255)          cAGGATACTGATGCGTTAAATGCGCATGTGCCCAGACGGAAGGTGTCACCAACGAAGTGGTCAGGATTGCCAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAAC‑tttttttac                                                                                                                               <  1:1098135/140‑4 (MQ=255)          cAGGATACTGATGCGTTAAATGCGCATGTGCCCAGACGGAAGGTGTCACCAACGAAGTGGTCAGGATTGCCAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAAC‑tttttttac                                                                                                                               <  1:959793/140‑4 (MQ=255)           aGGATACTGATGCGTTAAATGCGCATGTGCCCAGACGGAAGGTGTCACCAACGAAGTGGTCAGGATTGCCAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAAC‑tttttttaca                                                                                                                              <  2:1058722/140‑5 (MQ=255)           aGGATACTGATGCGTTAAATGCGCATGTGCCCAGACGGAAGGTGTCACCAACGAAGTGGTCAGGATTGCCAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAAC‑tttttttaca                                                                                                                              <  1:285410/140‑5 (MQ=255)                    aTGCGTTAAATGCGCATGTGCCCAGACGGAAGGTGTCACCAACGAAGTGGTCAGGATTGCCAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAAc                                                                                                                      >  1:257564/1‑140 (MQ=255)                      gCGTTAAATGCGCATGTGCCCAGACGGAAGGTGTCACCAACGAAGTGGTCAGGATTGCCAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCt                                                                                                                    <  1:365164/140‑1 (MQ=255)                       cGTTAAATGCGCATGTGCCCAGACGGAAGGTGTCACCAACGAAGTGGTCAGGATTGCCAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGc                                                                                                                           >  2:190820/1‑132 (MQ=255)                       cGTTAAATGCGCATGTGCCCAGACGGAAGGTGTCACCAACGAAGTGGTCAGGATTGCCAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGc                                                                                                                           <  1:190820/132‑1 (MQ=255)                       cGTTAAATGCGCATGTGCCCAGACGGAAGGTGTCACCAACGAAGTGGTCAGGATTGCCAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCtt                                                                                                                   >  1:603542/1‑140 (MQ=255)                       cGTTAAATGCGCATGTGCCCAGACGGAAGGTGTCACCAACGAAGTGGTCAGGATTGCCAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCt                                                                                                                    <  1:567679/139‑1 (MQ=255)                       cGTTAAATGCGCATGTGCCCAGACGGAAGGTGTCACCAACGAAGTGGTCAGGATTGCCAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCt                                                                                                                    >  2:567679/1‑139 (MQ=255)                         ttAAATGCGCATGTGCCCAGACGGAAGGTGTCACCAACGAAGTGGTCAGGATTGCCAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGt                                                                                                                 >  2:755738/1‑140 (MQ=255)                           aaaTGCGCATGTGCCCAGACGGAAGGTGTCACCAACGAAGTGGTCAGGATTGCCAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCt                                                                                                               >  1:957349/1‑140 (MQ=255)                                  cATGTGCCCAGACGGAAGGTGTCACCAACGAAGTGGTCAGGATTGCCAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCt                                                                                                        >  1:884456/1‑140 (MQ=255)                                  cATGTGCCCAGACGGAAGGTGTCACCAACGAAGTGGTCAGGATTGCCAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCt                                                                                                        <  2:440637/140‑1 (MQ=255)                                  cATGTGCCCAGACGGAAGGTGTCACCAACGAAGTGGTCAGGATTGCCAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCt                                                                                                        <  2:957349/140‑1 (MQ=255)                                  cATGTGCCCAGACGGAAGGTGTCACCAACGAAGTGGTCAGGATTGCCAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCt                                                                                                        <  1:755738/140‑1 (MQ=255)                                  cATGTGCCCAGACGGAAGGTGTCACCAACGAAGTGGTCAGGATTGCCAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCt                                                                                                        <  1:102076/140‑1 (MQ=255)                                   aTGTGCCCAGACGGAAGGTGTCACCAACGAAGTGGTCAGGATTGCCAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTg                                                                                                       >  2:320061/1‑140 (MQ=255)                                   aTGTGCCCAGACGGAAGGTGTCACCAACGAAGTGGTCAGGATTGCCAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTg                                                                                                       >  1:230260/1‑140 (MQ=255)                                      tgCCCAGACGGAAGGTGTCACCAACGAAGTGGTCAGGATTGCCAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTc                                                                                                         >  1:1317006/1‑135 (MQ=255)                                      tgCCCAGACGGAAGGTGTCACCAACGAAGTGGTCAGGATTGCCAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTc                                                                                                         <  2:1317006/135‑1 (MQ=255)                                      tgCCCAGACGGAAGGTGTCACCAACGAAGTGGTCAGGATTGCCAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGt                                                                                                    <  1:1258685/140‑1 (MQ=255)                                      tgCCCAGACGGAAGGTGTCACCAACGAAGTGGTCAGGATTGCCAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGt                                                                                                    >  2:340016/1‑140 (MQ=255)                                           aGACGGAAGGTGTCACCAACGAAGTGGTCAGGATTGCCAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAgg                                                                                               <  1:320061/140‑1 (MQ=255)                                                gAAGGTGTCACCAACGAAGTGGTCAGGATTGCCAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGatcat                                                                                          >  1:928950/1‑140 (MQ=255)                                                 aaGGTGTCACCAACGAAGTGGTCAGGATTGCCAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTTCAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGatcatc                                                                                         >  1:725751/1‑140 (MQ=255)                                                 aaGGTGTCACCAACGAAGTGGTCAGGATTGCCAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGatcatc                                                                                         >  1:558160/1‑140 (MQ=255)                                                       tCACCAACGAAGTGGTCAGGATTGCCAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTcc                                                                                   >  1:617024/1‑140 (MQ=255)                                                       tCACCAACGAAGTGGTCAGGATTGCCAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTcc                                                                                   >  2:934344/1‑140 (MQ=255)                                                        cACCAACGAAGTGGTCAGGATTGCCAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTCCg                                                                                  <  2:906245/140‑1 (MQ=255)                                                         aCCAACGAAGTGGTCAGGATTGCCAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTCCgg                                                                                 >  2:1467857/1‑140 (MQ=255)                                                           cAACGAAGTGGTCAGGATTGCCAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTCCGGCt                                                                               >  1:1418361/1‑140 (MQ=255)                                                             aCGAAGTGGTCAGGATTGCCAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTCCGGCTTg                                                                             <  2:603542/140‑1 (MQ=255)                                                             aCGAAGTGGTCAGGATTGCCAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTCCGGCTTg                                                                             <  2:884456/140‑1 (MQ=255)                                                             aCGAAGTGGTCAGGATTGCCAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTCCGGCTTg                                                                             <  1:977235/140‑1 (MQ=255)                                                              cGAAGTGGTCAGGATTGCCAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTCCGGCTTGc                                                                            >  1:818054/1‑140 (MQ=255)                                                               gAAGTGGTCAGGATTGCCAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTCCGGCTTGcc                                                                           >  1:721777/1‑140 (MQ=255)                                                                 aGTGGTCAGGATTGCCAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTCCGGCTTGCCAt                                                                         >  1:372131/1‑140 (MQ=255)                                                                  gTGGTCAGGATTGCCAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTCCGGCTTGCCATc                                                                        <  2:911107/140‑1 (MQ=255)                                                                     gTCAGGATTGCCAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTCCGGCTTGCCATc                                                                        <  1:1419955/137‑1 (MQ=255)                                                                     gTCAGGATTGCCAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTCCGGCTTGCCATc                                                                        >  2:1419955/1‑137 (MQ=255)                                                                      tCAGGATTGCCAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTCCGGCTTGCCATCCTgg                                                                    <  2:818054/140‑1 (MQ=255)                                                                        aGGATTGCCAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTCCGGCTTGCCATCCTGGCt                                                                  <  2:173413/140‑1 (MQ=255)                                                                        aGGATTGCCAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTCCGGCTTGCCATCCTGGCt                                                                  <  2:136286/140‑1 (MQ=255)                                                                          gATTGCCAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTCCGGCTTGCCATCCTGGCTCa                                                                <  2:458081/140‑1 (MQ=255)                                                                               ccAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTCCGGCTTGCCATCCTGGCTCa                                                                >  2:901424/1‑135 (MQ=255)                                                                               ccAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTCCGGCTTGCCATCCTGGCTCa                                                                <  1:901424/135‑1 (MQ=255)                                                                               ccAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTCCGGCTTGCCATCCTGGCTCACTCtt                                                           >  1:980919/1‑140 (MQ=255)                                                                               ccAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTCCGGCTTGCCATCCTGGCTCACTCtt                                                           >  1:614532/1‑140 (MQ=255)                                                                                   cgcgTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTCCGGCTTGCCATCCTGGCTCACTCTTAGTa                                                       >  1:1093142/1‑140 (MQ=255)                                                                                        aGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTCCGGCTTGCCATCCTGGCTCACTCTTAGTAACttt                                                  >  1:830110/1‑140 (MQ=255)                                                                                               ggAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTCCGGCTTGCCATCCTGGCTCACTCTTAGTAACTTTTGCCcgc                                           <  2:372131/140‑1 (MQ=255)                                                                                                  gCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTCCGGCTTGCCATCCTGGCTCACTCTTAGTAACTTTTGCCCGCGaa                                        >  1:825488/1‑140 (MQ=255)                                                                                                    gTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTCCGGCTTGCCATCCTGGCTCACTCTTAGTAACTTTTGCCCGCGAatg                                      >  2:716994/1‑140 (MQ=255)                                                                                                       cAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTCCGGCTTGCCATCCTGGCTCACTCTTAGTAACTTTTGCCCGCGAatgatg                                   <  2:825488/140‑1 (MQ=255)                                                                                                       cAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTCCGGCTTGCCATCCTGGCTCACTCTTAGTAACTTTTGCCCGCGAatgatg                                   <  2:558160/140‑1 (MQ=255)                                                                                                         gTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTCCGGCTTGCCATCCTGGCTCACTCTTAGTAACTTTTGCCCGCGAATGATgag                                 <  2:257564/140‑1 (MQ=255)                                                                                                         gTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTCCGGCTTGCCATCCTGGCTCACTCTTAGTAACTTTTGCCCGCGAATGATgag                                 <  2:830110/140‑1 (MQ=255)                                                                                                         gTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTCCGGCTTGCCATCCTGGCTCACTCTTAGTAACTTTTGCCCGCGAATGATgag                                 <  2:928950/140‑1 (MQ=255)                                                                                                         gTTGAAGCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTCCGGCTTGCCATCCTGGCTCACTCTTAGTAACTTTTGCCCGCGAATGATgag                                 <  2:721777/140‑1 (MQ=255)                                                                                                               gCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTCCGGCTTGCCATCCTGGCTCACTCTTAGTAACTTTTGCCCGCGAATGATgag                                 <  2:601270/134‑1 (MQ=255)                                                                                                               gCCATATTATCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTCCGGCTTGCCATCCTGGCTCACTCTTAGTAACTTTTGCCCGCGAATGATgag                                 >  1:601270/1‑134 (MQ=255)                                                                                                                    attatCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTCCGGCTTGCCATCCTGGCTCACTCTTAGTAACTTTTGCCCGCGAATGATGAGGAGATTAAGaa                      >  1:476915/1‑140 (MQ=255)                                                                                                                     ttatCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTCCGGCTTGCCATCCTGGCTCACTCTTAGTAACTTTTGCCCGCGAATGATGAGGAGATTAAGAAt                     <  2:980919/140‑1 (MQ=255)                                                                                                                      tatCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTCCGGCTTGCCATCCTGGCTCACTCTTAGTAACTTTTGCCCGCGAATGATGAGGAGATTAAGAATg                    <  2:617024/140‑1 (MQ=255)                                                                                                                      tatCTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTCCGGCTTGCCATCCTGGCTCACTCTTAGTAACTTTTGCCCGCGAATGATGAGGAGATTAAGAATg                    <  1:934344/140‑1 (MQ=255)                                                                                                                         cTATTCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTCCGGCTTGCCATCCTGGCTCACTCTTAGTAACTTTTGCCCGCGAATGATCAGGAGATTAAGAATGCTg                 <  2:614532/140‑1 (MQ=255)                                                                                                                             tCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTCCGGCTTGCCATCCTGGCTCACTCTTAGTAACTTTTGCCCGCGAATGATGAGGAGATTAAGAATGCTgaaga             >  1:185215/1‑140 (MQ=255)                                                                                                                             tCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTCCGGCTTGCCATCCTGGCTCACTCTTAGTAACTTTTGCCCGCGAATGATGAGGAGATTAAGAATGCTgaaga             >  1:1455298/1‑140 (MQ=255)                                                                                                                             tCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTCCGGCTTGCCATCCTGGCTCACTCTTAGTAACTTTTGCCCGCGAATGATGAGGAGATTAAGAATGCTgaaga             >  1:498/1‑140 (MQ=255)                                                                                                                             tCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTCCGGCTTGCCATCCTGGCTCACTCTTAGTAACTTTTGCCCGCGAATGATGAGGAGATTAAGAATGCTgaaga             >  2:112145/1‑140 (MQ=255)                                                                                                                             tCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTCCGGCTTGCCATCCTGGCTCACTCTTAGTAACTTTTGCCCGCGAATGATGAGGAGATTAAGAATGCTgaaga             >  2:656965/1‑140 (MQ=255)                                                                                                                             tCCTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTCCGGCTTGCCATCCTGGCTCACTCTTAGTAACTTTTGCCCGCGAATGATGAGGAGATTAAGAATGCTgaaga             >  2:1118358/1‑140 (MQ=255)                                                                                                                               cTTTTTGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTCCGGCTTGCCATCCTGGCTCACTCTTAGTAACTTTTGCCCGCGAATGATGAGGAGATTAAGAATGCTGAAGAAt           <  1:112145/140‑1 (MQ=255)                                                                                                                                tttttGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTCCGGCTTGCCATCCTGGCTCACTCTTAGTAACTTTTGCCCGCGAATGATGAGGAGATTAAGAATGCTGAAGAATc          >  1:848479/1‑140 (MQ=255)                                                                                                                                  tttGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTCCGGCTTGCCATCCTGGCTCACTCTTAGTAACTTTTGCCCGCGAATGATGAGGAGATTAAGAATGCTGAAGAATCTg        >  2:796401/1‑140 (MQ=255)                                                                                                                                   ttGTAATAACTTTTTTTTCAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTCCGGCTTGCCATCCTGGCTCACTCTTAGTAACTTTTGCCCGCGAATGATGAGGAGATTAAGAATGCTGAAGAATCTgg       <  2:725751/140‑1 (MQ=255)                                                                                                                                   ttGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTCCGGCTTGCCATCCTGGCTCACTCTTAGTAACTTTTGCCCGCGAATGATGAGGAGATTAAGAATGCTGAAGAATCTgg       <  1:656965/140‑1 (MQ=255)                                                                                                                                    tGTAATAACTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTCCGGCTTGCCATCCTGGCTCACTCTTAGTAACTTTTGCCCGCGAATGATGAGGAGATTAAGAATGCTGAAGAATCTGGc      >  2:736345/1‑140 (MQ=255)                                                                                                                                        ataaCTTTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTCCGGCTTGCCATCCTGGCTCACTCTTAGTAACTTTTGCCCGCGAATGATGAGGAGATTAAGAATGCTGAAGAATCTGGCTaaa  <  1:796401/140‑1 (MQ=255)                                                                                                                                             |                                                                                                                                       CGTTTGCCGCAGGATACTGATGCGTTAAATGCGCATGTGCCCAGACGGAAGGTGTCACCAACGAAGTGGTCAGGATTGCCAGCGCGTAGCGAAGGGAGCGTGCAGTTGAAGCCATATTATCTATTCCTTTTTGTAATAAC‑TTTTTTACAGAGCATAACCTTGTCTAATGTCTGAGTCGAGGATCATCAATTCCGGCTTGCCATCCTGGCTCACTCTTAGTAACTTTTGCCCGCGAATGATGAGGAGATTAAGAATGCTGAAGAATCTGGCTAAA  >  NC_000913/1924855‑1925128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑