BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,931,192	0	C	T	56.4%	37.9 / 124.1	101	C104C (TGC→TGT)	purT	phosphoribosylglycinamide formyltransferase 2
Reads supporting (aligned to +/- strand): ref base C (24/20); new base T (32/25); total (56/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.09e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 105 total aligned reads displayed.

AATATGCTTGATGGTGATGCATTACGCCGTGTGGTTGAACTGGAAAAACCACATTATATCGTGCCGGAGATCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGCGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGAGCTGCAGCTGCCCACTTCCACTTATCGTTTTGCCGATAGCGAAAGCCTTTTCCGCGAGGCGGTTGCTGACATTGGCTATC  >  NC_000913/1931058‑1931331

aaTATGCTTGATGGTGATGCATTACGCCGTGTGGTTGAACTGGAAAAACCACATTATATCGTGCCGGAGATCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGTGCTcg                                                                                                                                        <  2:1078250/140‑1 (MQ=255)

   atGCTTGATGGTGATGCATTACGCCGTGTGGTTGAACTGGAAAAACCACATTATATCGTGCCGGAGATCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGTGCTCgcgc                                                                                                                                     >  2:1341841/1‑140 (MQ=255)

     gCTTGATGGTGATGCATTACGCCGTGTGGTTGAACTGGAAAAACCACATTATATCGTGCCGGAGATCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGTGCTCGCGCaa                                                                                                                                   >  1:1364826/1‑140 (MQ=255)

     gCTTGATGGTGATGCATTACGCCGTGTGGTTGAACTGGAAAAACCACATTATATCGTGCCGGAGATCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGCGCTCGCGCaa                                                                                                                                   >  2:393838/1‑140 (MQ=255)

      cTTGATGGTGATGCATTACGCCGTGTGGTTGAACTGGAAAAACCACATTATATCGTGCCGGAGATCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGTGCTCGCGCAAc                                                                                                                                  >  1:230422/1‑140 (MQ=255)

      cTTGATGGTGATGCATTACGCCGTGTGGTTGAACTGGAAAAACCACATTATATCGTGCCGGAGATCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGCGCTCGCGCAAc                                                                                                                                  <  2:566065/140‑1 (MQ=255)

                aTGCATTACGCCGTGTGGTTGAACTGGAAAAACCACATTATATCGTGCCGGAGATCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGTGCTCGCGCAACGAAATTAACg                                                                                                                        >  2:933930/1‑140 (MQ=255)

                   cATTACGCCGTGTGGTTGAACTGGAAAAACCACATTATATCGTGCCGGAGATCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGTGCTCGCGCAACGAAATTAACGATg                                                                                                                     <  2:230422/140‑1 (MQ=255)

                     ttACGCCGTGTGGTTGAACTGGAAAAACCACATTATATCGTGCCGGAGATCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGTGCTCGCGCAACGAAATTAACGATGaa                                                                                                                   <  2:1016672/140‑1 (MQ=255)

                     ttACGCCGTGTGGTTGAACTGGAAAAACCACATTATATCGTGCCGGAGATCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGTGCTCGCGCAACGAAATTAACGATGaa                                                                                                                   >  1:300219/1‑140 (MQ=255)

                     ttACGCCGTGTGGTTGAACTGGAAAAACCACATTATATCGTGCCGGAGATCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGCGCTCGCGCAACGAAATTAACGATGaa                                                                                                                   >  2:861018/1‑140 (MQ=255)

                        cGCCGTGTGGTTGAACTGGAAAAACCACATTATATCGTGCCGGAGATCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGTGCTCGCGCAACGAAATTAACGATGAATcg                                                                                                                >  2:370273/1‑140 (MQ=255)

                        cGCCGTGTGGTTGAACTGGAAAAACCACATTATATCGTGCCGGAGATCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGCGCTCGCGCAACGAAATTAACGATGAATcg                                                                                                                >  2:1234565/1‑140 (MQ=255)

                          ccGTGTGGTTGAACTGGAAAAACCACATTATATCGTGCCGGAGATCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGTGCTCGCGCAACGAAATTAACGATGAATcgcg                                                                                                              <  2:300219/140‑1 (MQ=255)

                           cGTGTGGTTGAACTGGAAAAACCACATTATATCGTGCCGGAGATCGAAGCTATTGCCACCGATATGCTTATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGCGCTCGCGCAACGAAATTAACGATGAATCGCGa                                                                                                             >  1:1403052/1‑140 (MQ=255)

                            gtgtGGTTGAACTGGAAAAACCACATTATATCGTGCCGGAGATCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGCGCTCGCGCAACGAAATTAACGATGAATCGCGAg                                                                                                            >  2:558374/1‑140 (MQ=255)

                             tgtgGTTGAACTGGAAAAACCACATTATGTCGTGCCGTAGATCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGCGATCGCGCAACGAAATTAACGATGAATCGCGAgg                                                                                                           >  2:1305944/1‑140 (MQ=255)

                             tgtgGTTGAACTGGAAAAACCACATTATATCGTGCCGGAGATCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGCGCTCGCGCAACGAAATTAACGATGAAt                                                                                                                  >  2:1162822/1‑133 (MQ=255)

                             tgtgGTTGAACTGGAAAAACCACATTATATCGTGCCGGAGATCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGCGCTCGCGCAACGAAATTAACGATGAAt                                                                                                                  <  1:1162822/133‑1 (MQ=255)

                             tgtgGTTGAACTGGAAAAACCACATTATATCGTGCCGGAGATCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGCGCTCGCGCAACGAAATTAACGATGAATcgc                                                                                                               <  1:1096185/136‑1 (MQ=255)

                             tgtgGTTGAACTGGAAAAACCACATTATATCGTGCCGGAGATCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGCGCTCGCGCAACGAAATTAACGATGAATcgc                                                                                                               >  2:1096185/1‑136 (MQ=255)

                             tgtgGTTGAACTGGAAAAACCACATTATATCGTGCCGGAGATCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGCGCTCGCGCAACGAAATTAACGATGAATcg                                                                                                                <  1:384046/135‑1 (MQ=255)

                             tgtgGTTGAACTGGAAAAACCACATTATATCGTGCCGGAGATCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGCGCTCGCGCAACGAAATTAACGATGAATcg                                                                                                                >  2:384046/1‑135 (MQ=255)

                             tgtgGTTGAACTGGAAAAACCACATTATATCGTGCCGGAGATCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGCGCTCGCGCAACGAAATTAACGATGAATCGCGAgg                                                                                                           >  1:1230865/1‑140 (MQ=255)

                              gtgGTTGAACTGGAAAAACCACATTATATCGTGCCGGAGATCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGCGCTCGCGCAACGAAATTAACGATGAATCGCGAggg                                                                                                          <  1:393838/140‑1 (MQ=255)

                                  ttGAACTGGAAAAACCACATTATATCGTGCCGGAGATCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGCGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATc                                                                                                      >  2:486450/1‑140 (MQ=255)

                                    gAACTGGAAAAACCACATTATATCGTGCCGGAGATCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGTGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCg                                                                                                    >  1:1351496/1‑140 (MQ=255)

                                       cTGGAAAAACCACATTATATCGTGCCGGAGATCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGTGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCg                                                                                                 >  2:1390058/1‑140 (MQ=255)

                                        tGGAAAAACCACATTATATCGTGCCGGAGATCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGTGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGc                                                                                                <  2:1364826/140‑1 (MQ=255)

                                           aaaaaCCACATTATATCGTGCCGGAGATCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGCGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTg                                                                                             >  2:1336993/1‑140 (MQ=255)

                                               aCCACATTATATCGTGCCGGAGATCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGTGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTggcgg                                                                                         >  2:1459806/1‑140 (MQ=255)

                                                 cacaTTATATCGTGCCGGAGATCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGTGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCa                                                                                       >  1:373257/1‑140 (MQ=255)

                                                  acaTTATATCGTGCCGGAGATCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGTGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCag                                                                                      <  2:373257/140‑1 (MQ=255)

                                                   caTTATATCGTGCCGGAGATCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGCGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCaga                                                                                     <  1:1336993/140‑1 (MQ=255)

                                                    aTTATATCGTGCCGGAGATCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGCGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCagaa                                                                                    >  2:632180/1‑140 (MQ=255)

                                                     ttATATCGTGCCGGAGATCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGCGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCt                                                                                              <  2:974235/129‑1 (MQ=255)

                                                     ttATATCGTGCCGGAGATCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGCGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCt                                                                                              <  2:188366/129‑1 (MQ=255)

                                                     ttATATCGTGCCGGAGATCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGCGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCt                                                                                              >  1:974235/1‑129 (MQ=255)

                                                     ttATATCGTGCCGGAGATCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGCGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCt                                                                                              >  1:188366/1‑129 (MQ=255)

                                                           cGTGCCGGAGATCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGTGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGagctgc                                                                             >  1:11874/1‑140 (MQ=255)

                                                             tGCCGGAGATCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGTGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGagctgcag                                                                           >  2:293920/1‑140 (MQ=255)

                                                             tGCCGGAGATCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGTGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGagctgcag                                                                           <  1:1459806/140‑1 (MQ=255)

                                                             tGCCGGAGATCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGTGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGagctgcag                                                                           >  1:766139/1‑140 (MQ=255)

                                                             tGCCGGAGATCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGCGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGagctgcag                                                                           <  1:1234565/140‑1 (MQ=255)

                                                              gCCGGAGATCGACGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGTGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGCATCCGTCGCCTGGCGGCAGAAGagctgcagc                                                                          >  2:808396/1‑140 (MQ=255)

                                                              gCCGGAGATCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGTGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGagctgcagc                                                                          <  1:933930/140‑1 (MQ=255)

                                                              gCCGGAGATCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGTGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGagctgcagc                                                                          >  2:588869/1‑140 (MQ=255)

                                                              gCCGGAGATCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGTGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGagctgcag                                                                           <  2:1301507/139‑1 (MQ=255)

                                                              gCCGGAGATCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGTGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGagctgcag                                                                           >  1:1301507/1‑139 (MQ=255)

                                                                cGGAGATCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGTGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGagctgcagctg                                                                        <  1:370273/140‑1 (MQ=255)

                                                                cGGAGATCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGACGAAGGACTGAATGTTGTCCCCTGTGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGagctgcagctg                                                                        <  2:819463/140‑1 (MQ=255)

                                                                  gagaTCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGTGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGAGCTGCAGCTGcc                                                                      >  2:212724/1‑140 (MQ=255)

                                                                  gagaTCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGTGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGAGCTGCAGCTGcc                                                                      <  1:1373472/140‑1 (MQ=255)

                                                                  gagaTCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGCGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGAGCTGCAGCTGcc                                                                      <  1:1305944/140‑1 (MQ=255)

                                                                      tCGAAGCTATTGCCACCGATATGCTTATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGCGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGAGCTGCAGCTGCccact                                                                  <  2:1403052/140‑1 (MQ=255)

                                                                              aTTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGTGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGAGCTGCAGCTGCCCACTTCCACTTa                                                          >  2:602038/1‑140 (MQ=255)

                                                                              aTTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGCGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGAGCTGCAGCTGCccact                                                                  <  2:368845/132‑1 (MQ=255)

                                                                              aTTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGCGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGAGCTGCAGCTGCccact                                                                  >  1:368845/1‑132 (MQ=255)

                                                                               ttGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGTGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGAGCTGCAGCTGCCCACTTCCACTTAt                                                         >  1:256551/1‑140 (MQ=255)

                                                                                tGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGCGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGAGCTGCAGCTGCCCACTTCCACTTATc                                                        >  1:402664/1‑140 (MQ=255)

                                                                                    aCCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGTGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGAGCTGCAGCTGCCCACTTCCACTTATCGttt                                                    >  2:153064/1‑140 (MQ=255)

                                                                                         tatGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGCGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGAGCTGCAGCTGCCCACTTCCACTTATCGTTTTGCCg                                               >  2:1159512/1‑140 (MQ=255)

                                                                                          atGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGTGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGAGCTGCAGCTGCCCACTTCCACTTATCGTTTTGCCGa                                              <  2:766139/140‑1 (MQ=255)

                                                                                          atGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGTGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGAGCTGCAGCTGCCCACTTCCACTTATCGTTTTGCCGa                                              <  1:462124/140‑1 (MQ=255)

                                                                                            gCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGTGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGAGCTGCAGCTGCCCACTTCCACTTATCGTTTTGCCGATa                                            >  1:29555/1‑140 (MQ=255)

                                                                                               gATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGTGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGAGCTGCAGCTGCCCACTTCCACTTATCGTTTTGCCGATAGca                                         <  2:1351496/140‑2 (MQ=255)

                                                                                               gATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGCGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGAGCTGCAGCTGCCCACTTCCACTTATCGTTTTGCCGATAGCg                                         <  1:632180/140‑1 (MQ=255)

                                                                                                aTCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGTGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGAGCTGCAGCTGCCCACTTCCACTTATCGTTTTGCCGATAGCGa                                        >  1:1008990/1‑140 (MQ=255)

                                                                                                 tCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGCGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGAGCTGCAGCTGCCCACTTCCACTTATCGTTTTGCCGATAGCGaa                                       >  2:881779/1‑140 (MQ=255)

                                                                                                  ccAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGTGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGAGCTGCAGCTGCCCACTTCCACTTATCGTTTTGCCGATAGCGaaa                                      >  2:118890/1‑140 (MQ=255)

                                                                                                     aCTTGAAGAGGAAGGACTGAATGTTGTCCCCTGCGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGAGCTGCAGCTGCCCACTTCCACTTATCGTTTTGCCGATAGCGAAAGcc                                   >  1:814854/1‑140 (MQ=255)

                                                                                                       ttGAAGAGGAAGGACTGAATGTTGTCCCCTGCGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGAGCTGCAGCTGCCCACTTCCACTTATCGTTTTGCCGATAGCGAAAGCCtt                                 >  2:473457/1‑140 (MQ=255)

                                                                                                          aaGAGGAAGGACTGAATGTTGTCCCCTGCGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGAGCTGCAGCTGCCCACTTCCACTTATCGTTTTGCCGATAGCGAAAGCCTTTTc                              <  1:861018/140‑1 (MQ=255)

                                                                                                               gaaggaCTGAATGTTGTCCCATGTGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGAGCTGCAGCTGCCCACTTCCACTTATCGTTTTGCCGATAGCg                                         <  2:907456/124‑1 (MQ=255)

                                                                                                               gaaggaCTGAATGTTGTCCCATGTGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGAGCTGCAGCTGCCCACTTCCACTTATCGTTTTGCCGATAGCg                                         >  1:907456/1‑124 (MQ=255)

                                                                                                                 aggaCTGAATGTTGTCCCCTGTGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGAGCTGCAGCTGCCCACTTCCACTTATCGTTTTGCCGATAGCGAAAGCCTTTTCCGCGAgg                       <  2:29555/140‑1 (MQ=255)

                                                                                                                 aggaCTGAATGTTGTCCCCTGTGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGAGCTGCAGCTGCCCACTTCCACTTATCGTTTTGCCGATAGCGAAAGCCTTTTCCGCGAgg                       <  2:256551/140‑1 (MQ=255)

                                                                                                                 aggaCTGAATGTTGTCCCCTGCGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGAGCTGCAGCTGCCCACTTCCACTTATCGTTTTGCCGATAGCGAAAGCCTTTTCCGCGAgg                       <  2:800115/140‑1 (MQ=255)

                                                                                                                 aggaCTGAATGTTGTCCCCTGCGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGAGCTGCAGCTGCCCACTTCCACTTATCGTTTTGCCGATAGCGAAAGCCTTTTCCGCGAgg                       >  2:389434/1‑140 (MQ=255)

                                                                                                                  ggaCTGAATGTTGTCCCCTGTGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGAGCTGCAGCTGCCCACTTCCACTTATCGTTTTGCCGATAGCGAAAGCCTTTTCCGCGAGGc                      <  2:1140945/140‑1 (MQ=255)

                                                                                                                    aCTGAATGTTGTCCCCTGTGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGAGCTGCAGCTGCCCACTTCCACTTATCGTTTTGCCGATAGCGAAAGCCTTTTCCGCGAGGCgg                    <  1:212724/140‑1 (MQ=255)

                                                                                                                       gAATGTTGTCCCCTGCGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGAGCTGCAGCTGCCCACTTCCACTTATCGTTTTGCCGATAGCGAAAGCCTTTTCCGCGAGGCGGTTg                 <  1:389434/140‑1 (MQ=255)

                                                                                                                        aaTGTTGTCCCCTGTGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGAGCTGCAGCTGCCCACTTCCACTTATCGTTTTGCCGATAGCGAAAGCCTTTTCCGCGAGGCGGTTGc                >  2:928935/1‑140 (MQ=255)

                                                                                                                        aaTGTTGTCCCCTGTGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGAGCTGCAGCTGCCCACTTCCACTTATCGTTTTGCCGATAGCGAAAGCCTTTTCCGCGAGGCGGTTGc                >  2:1088068/1‑140 (MQ=255)

                                                                                                                        aaTGTTGTCCCCTGTGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGAGCTGCAGCTGCCCACTTCCACTTATCGTTTTGCCGATAGCGAAAGCCTTTTCCGCGAGGCGGTTGc                >  1:904180/1‑140 (MQ=255)

                                                                                                                        aaTGTTGTCCCCTGTGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGAGCTGCAGCTGCCCACTTCCACTTATCGTTTTGCCGATAGCGAAAGCCTTTTCCGCGAGGCGGTTGc                >  1:803506/1‑140 (MQ=255)

                                                                                                                         aTGTTGTCCCCTGTGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGAGCTGCAGCTGCCCACTTCCACTTATCGTTTTGCCGATAGCGACAGCCTTTTCCGCGAGGCg                     >  2:860819/1‑134 (MQ=255)

                                                                                                                         aTGTTGTCCCCTGTGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGAGCTGCAGCTGCCCACTTCCACTTATCGTTTTGCCGATAGCGAAAGCCTTTTCCGCGAGGCg                     <  1:860819/134‑1 (MQ=255)

                                                                                                                           gttgtCCCCTGTGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGAGCTGCAGCTGCCCACTTCCACTTATCGTTTTGCCGATAGCGAAAGCCTTTTCCGCGAGGCGGTTGCTGa             <  1:602038/140‑1 (MQ=255)

                                                                                                                             tgtCCCCTGTGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGAGCTGCAGCTGCCCACTTCCACTTATCGTTTTGCCGATAGCGAAAGCCTTTTCCGCGAGGCGGTTGCTGACa           <  2:1008990/140‑1 (MQ=255)

                                                                                                                             tgtCCCCTGCGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGAGCTGCAGCTGCCCACTTCCACTTATCGTTTTGCCGATAGCGAAAGCCTTTTCCGCGAGGCGGTTGCTGACa           >  1:1059251/1‑140 (MQ=255)

                                                                                                                             tgtCCCCTGCGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGAGCTGCAGCTGCCCACTTCCACTTATCGTTTTGCCGATAGCGAAAGCCTTTTCCGCGAGGCGGTTGCTGACa           <  2:1059251/140‑1 (MQ=255)

                                                                                                                               tCCCCTGTGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGAGCTGCAGCTGCCCACTTCCACTTATCGTTTTGCCGATAGCGAAAGCCTTTTCCGCGAGGCGGTTGCTGACAtt         >  1:1228953/1‑140 (MQ=255)

                                                                                                                                ccccTGTGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGAGCTGCAGCTGCCCACTTCCACTTATCGTTTTGCCGATAGCGAAAGCCTTTTCCGCGAGGCGGTTGCTGACATTg        <  1:153064/140‑1 (MQ=255)

                                                                                                                                  ccTGTGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGAGCTGCAGCTGCCCACTTCCACTTATCGTTTTGCCGATAGCGAAAGCCTTTTCCGCGAGGCGGTTGCTGACATTGGc      <  1:1390058/140‑1 (MQ=255)

                                                                                                                                  ccTGTGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGAGCTGCAGCTGCCCACTTCCACTTATCGTTTTGCCGATAGCGAAAGCCTTTTCCGCGAGGCGGTTGCTGACATTGGc      <  2:312301/140‑1 (MQ=255)

                                                                                                                                  ccTGCGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGAGCTGCAGCTGCCCACTTCCACTTATCGTTTTGCCGATAGCGAAAGCCTTTTCCGCGAGGCGGTTGCTGACATTGGc      <  2:814854/140‑1 (MQ=255)

                                                                                                                                  ccTGCGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGAGCTGCAGCTGCCCACTTCCACTTATCGTTTTGCCGATAGCGAAAGCCTTTTCCGCGAGGCGGTTGCTGACATTGGc      <  1:1159512/140‑1 (MQ=255)

                                                                                                                                      cgcTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGAGCTGCAGCTGCCCACTTCCACTTATCGTTTTGCCGATAGCGAAAGCCTTTTCCGCGAGGCGGTTGCTGACATTGGCTATc  >  2:1453098/1‑140 (MQ=255)

                                                                                                                                      cgcTCGCGCAACGAAATTAACGATGAATCGCGAGGGGATCCGTCGCCTGGCGGCAGAAGAGCTGCAGCTGCCCACTTCCACTTATCGTTTTGCCGATAGCGAAAGCCTTTTCCGCGAGGCGGTTGCTGACATTGGGTATc  >  2:514771/1‑140 (MQ=255)

AATATGCTTGATGGTGATGCATTACGCCGTGTGGTTGAACTGGAAAAACCACATTATATCGTGCCGGAGATCGAAGCTATTGCCACCGATATGCTGATCCAACTTGAAGAGGAAGGACTGAATGTTGTCCCCTGCGCTCGCGCAACGAAATTAACGATGAATCGCGAGGGTATCCGTCGCCTGGCGGCAGAAGAGCTGCAGCTGCCCACTTCCACTTATCGTTTTGCCGATAGCGAAAGCCTTTTCCGCGAGGCGGTTGCTGACATTGGCTATC  >  NC_000913/1931058‑1931331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`