Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,404,215	0	T	G	29.2%	111.1 / 27.2	66	G244G (GGT→GGG)	yhdH	acrylyl‑CoA reductase
Reads supporting (aligned to +/- strand):  ref base T (3/43);  new base G (19/0);  total (23/43)
Fisher's exact test for biased strand distribution p-value = 1.25e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCC‑GTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCATTCT  >  NC_000913/3404079‑3404354                                                                                                                                         |                                                                                                                                             atgaGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCggggggg                                                                                                                                           >  1:1171635/1‑139 (MQ=255)  tgaGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCgggggggt                                                                                                                                          >  2:1003850/1‑138 (MQ=255)     gTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTa                                                                                                                                       <  1:1041823/140‑1 (MQ=255)     gTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTa                                                                                                                                       <  2:546530/140‑1 (MQ=255)      tttGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTAc                                                                                                                                      <  1:860214/140‑1 (MQ=255)      tttGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGgttttt                                                                                                                                      >  2:590904/1‑138 (MQ=255)        tGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTACGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTtctc                                                                                                                                    >  2:1018645/1‑140 (MQ=255)          ccGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTg                                                                                                                                  <  1:949299/140‑1 (MQ=255)          ccGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTg                                                                                                                                  <  2:501151/140‑1 (MQ=255)          ccGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTg                                                                                                                                  <  2:927011/140‑1 (MQ=255)          ccGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTg                                                                                                                                  <  2:504296/140‑1 (MQ=255)              aTCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTGCCaa                                                                                                                              >  2:347801/1‑140 (MQ=255)               tCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTACTCTTCCCAc                                                                                                                             >  2:429234/1‑140 (MQ=255)                cccGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAAcc                                                                                                                            >  1:309654/1‑140 (MQ=255)                 ccGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCa                                                                                                                           <  1:770805/140‑1 (MQ=255)                    tCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTACTCTGCCAACCAccg                                                                                                                        >  1:330157/1‑138 (MQ=255)                       tctGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCa                                                                                                                     <  1:1456537/140‑1 (MQ=255)                        ctGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCGAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCAt                                                                                                                    <  1:841199/140‑1 (MQ=255)                        ctGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCAt                                                                                                                    <  1:434072/140‑1 (MQ=255)                         tGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCAt                                                                                                                    <  1:516869/139‑1 (MQ=255)                         tGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTACTCTGCCAACCACGGTCAt                                                                                                                    >  2:516869/1‑139 (MQ=255)                                     tCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTg                                                                                                       >  1:1359817/1‑140 (MQ=255)                                     tCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTGCCAACCACGGTCATGCCATTTATTTTg                                                                                                       >  1:898807/1‑140 (MQ=255)                                     tCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTACTCTGCCAACCACGGGCATGCCATTTAttttt                                                                                                       >  1:695271/1‑137 (MQ=255)                                       tgggctgggGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCg                                                                                                     <  2:630819/140‑1 (MQ=255)                                        gggctgggGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGCGGGTTTTATCTGCCAACCACGGTCATGCCATTTTTTTTGCgg                                                                                                    >  2:1365556/1‑139 (MQ=255)                                              gggCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTGCCAACCCCGGTCCTGGCATTTTTTTTGCGGaaattc                                                                                              >  2:1152392/1‑136 (MQ=255)                                              gggCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGGCTGGCGGGGGGGTTTACTCTGCCAACCACCGTCATGCCATTTATTCTTCGGAAAGTc                                                                                              >  1:1425070/1‑140 (MQ=255)                                               ggCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTTTCTGCCCACCACGGGCATGCCCTTTTTTTTTCGTAAAGTcc                                                                                             >  1:371568/1‑140 (MQ=255)                                                   aTTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCC‑Gttt                                                                                        >  1:1408296/1‑140 (MQ=255)                                                   aTTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTTCCCAACACGGGCCTGCCCTTTTTTTTTCGTAATGTCC‑Ggtt                                                                                        >  2:697102/1‑140 (MQ=255)                                                   aTTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTGCCAACCACGGTCATGCCCTTTTTTCTGCGTAAAGTTC‑Gttt                                                                                        >  2:1445046/1‑140 (MQ=255)                                                     tGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCC‑GTTTGc                                                                                      <  2:1452168/140‑1 (MQ=255)                                                     tGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCC‑GTTTGc                                                                                      <  2:157544/140‑1 (MQ=255)                                                     tGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCC‑GTTTGc                                                                                      <  2:1234947/140‑1 (MQ=255)                                                      gACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCC‑GTTTGCa                                                                                     <  1:431218/140‑1 (MQ=255)                                                      gACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCC‑GTTTGCa                                                                                     <  2:1359817/140‑1 (MQ=255)                                                       acacCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCC‑GTTTGCaa                                                                                    <  1:1371451/140‑1 (MQ=255)                                                         acCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTTCCAACCACGGTCATTCCATTTATTCTGCGTAATGTCC‑GGTTGCCAgg                                                                                  >  1:590862/1‑140 (MQ=255)                                                         acCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTACTCTGCCAACCACGGGCATGCCATTTTTTTTTCGTAATGTCC‑GGTTTCCAgg                                                                                  >  2:250510/1‑140 (MQ=255)                                                          ccGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCC‑GTTTGCAAggg                                                                                 <  1:60806/140‑1 (MQ=255)                                                          ccGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCC‑GTTTGCAAggg                                                                                 <  1:1445046/140‑1 (MQ=255)                                                           cGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTGCCAACCACGGGCATGCCCTTTTTTCTGCGGAATGTCC‑GTTTGCCAgggg                                                                                >  1:312620/1‑140 (MQ=255)                                                            gTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCC‑GTTTGCAAGGGGt                                                                               <  2:309654/140‑1 (MQ=255)                                                            gTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCC‑GTTTGCAAGGGGt                                                                               <  2:1393721/140‑1 (MQ=255)                                                            gTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCC‑GTTTGCAAGGGGt                                                                               <  1:1399754/140‑1 (MQ=255)                                                              tGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTTGCAACCACCGTCATGCCCTTTTTTCTGCGTTATTTCCGGTTTGTAAgggtgg                                                                              >  2:60806/1‑137 (MQ=255)                                                              tGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTGCCAACCACGGGCATGCCATTTATTTTGCGTAATGTCC‑GTTTGCCAGgggggg                                                                             >  2:53450/1‑140 (MQ=255)                                                               ggCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCC‑GTTTGCAAGGGGTGGa                                                                            <  2:798283/140‑1 (MQ=255)                                                               ggCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCC‑GTTTGCAAGGGGTGGa                                                                            <  2:1408296/140‑1 (MQ=255)                                                               ggCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCC‑GTTTGCAAGGGGTGGa                                                                            <  1:347801/140‑1 (MQ=255)                                                               ggCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCC‑GTTTGCAAGGGGTGGa                                                                            <  1:1365556/140‑1 (MQ=255)                                                                          gCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTTCTCTGCCAACCACGGTCATGCCATTTTTTTTGCGGAATGTCC‑GTTTTCAAGGGGGGGGTTCAGTAAAGa                                                                 >  2:1167446/1‑140 (MQ=255)                                                                           cTGGCAAAAGTGCTGGCGCAAGTGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCC‑GTTTGCAAGGGGTGGATTCAGTAATGAc                                                                <  2:305219/140‑1 (MQ=255)                                                                           cTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTACTCTGCCAACCACGGGCATGCCATTTTTTTTGCGTAATGTCC‑GTTTGCAAGGGGGGGGTTCAGTAATGAc                                                                >  2:412940/1‑140 (MQ=255)                                                                           atGGCAAAAGTGCTGGCCGAAATGCATCACGGCGGCTGCGTAGCGGCTTGTGGTCTGACGGGTGGTTTTCCTCTGACAAACAAGGTCATGCCATTTATTCTGCGTGATGTCC‑GTTTGCAAGGGGTGGATTCAGTAATGAc                                                                <  2:872697/139‑1 (MQ=255)                                                                                   aGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTTCCAACCACGGGCCTGCCCTTTTTTTTGGGTAATTTCC‑CTTTTCAAGGGGGGGGTTCAGTAATTACGcccccac                                                        >  2:262673/1‑139 (MQ=255)                                                                                   aGTGCTGGCGCAAATGAATTACGGCGGCTGCGGGGCGGCCTGTGGGCTGGCGGGGGGGTTTACTCTGCCCACCACGGGCCTTGCCTTTTTTCTTCGGAATGTCC‑GTTTGCAAGGGGGGGGTTCAGTAATGAAGCCACCAg                                                        >  2:640126/1‑140 (MQ=255)                                                                                             cAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCC‑GTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCgcgc                                              <  1:250510/140‑1 (MQ=255)                                                                                               aaTGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCC‑GTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGcac                                            <  1:797260/140‑1 (MQ=255)                                                                                               aaTGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCC‑GTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGcac                                            <  2:371568/140‑1 (MQ=255)                                                                                                          gcggcTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTGCCAACCACGGGCATGCCATTTTTTTTGCGTAATGTCC‑GTTTTCAAGGGGGGGGTTCAGGAAAGACGCCACCAGAAAGCCGCGCACAAACCCGGCAg                                 >  2:1191565/1‑140 (MQ=255)                                                                                                            ggcTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTGCCAACCACGGTCATGCCCTTTATTCTGCGTAATGTCC‑GTTTGCAAGGGGGGGGTTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCCGGCAGCg                               >  2:771278/1‑140 (MQ=255)                                                                                                             gcTGCGTGGCGGCCTGTGGTCTGGGGGGGGGGTTTTTTTTGCCAACCACGGGCATCGCATTTTTTTTGTGTGAAGGCC‑GCTTTCTAGGGGGGGGTTCTGGAAAGAGCCCGCCAAAAAGCCCCCCCCAAGCCTTGCAGccg                              >  2:606609/1‑138 (MQ=255)                                                                                                             gcTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCC‑GTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGa                              <  1:771278/140‑1 (MQ=255)                                                                                                             gcTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCC‑GTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGa                              <  2:330157/140‑1 (MQ=255)                                                                                                                       ggcCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCC‑GTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCg                    <  1:606609/140‑1 (MQ=255)                                                                                                                             tgGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCC‑GTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTAc              <  1:697102/140‑1 (MQ=255)                                                                                                                                tCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCC‑GTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCgg           <  1:41258/140‑1 (MQ=255)                                                                                                                                  tGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCC‑GTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGaa         <  1:1300083/140‑1 (MQ=255)                                                                                                                                  tGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCC‑GTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGaa         <  1:1167446/140‑1 (MQ=255)                                                                                                                                  tGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCC‑GTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGaa         >  2:1222597/1‑140 (MQ=255)                                                                                                                                   ggCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCC‑GTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAAt        <  1:429234/140‑1 (MQ=255)                                                                                                                                   ggCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCC‑GTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAAt        <  2:159470/140‑1 (MQ=255)                                                                                                                                   ggCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCC‑GTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAAt        <  2:590862/140‑1 (MQ=255)                                                                                                                                   ggCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCC‑GTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAAt        <  2:1182467/140‑1 (MQ=255)                                                                                                                                     cGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCC‑GTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCa      <  2:1425070/140‑1 (MQ=255)                                                                                                                                       ggtggtTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCC‑GTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGaa         >  2:940065/1‑135 (MQ=255)                                                                                                                                       ggtggtTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCC‑GTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGaa         <  1:940065/135‑1 (MQ=255)                                                                                                                                       ggtggtTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCC‑GTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCAtt    <  1:412940/140‑1 (MQ=255)                                                                                                                                        gtggtTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCC‑GTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCATTc   <  2:1171635/140‑1 (MQ=255)                                                                                                                                         tggtTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCC‑GTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCATTCt  >  2:1200340/1‑140 (MQ=255)                                                                                                                                         tggtTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCC‑GTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCATTCt  >  1:1078687/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                             ATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCC‑GTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCATTCT  >  NC_000913/3404079‑3404354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑