Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	100,328	G→A	A229T (GCG→ACG)	murG →	N‑acetylglucosaminyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	100,328	0	G	A	100.0%	206.1 / NA	59	A229T (GCG→ACG)	murG	N‑acetylglucosaminyl transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/27);  total (32/27)

TGTGCTGGTAGTGGGTGGTTCTCAGGGCGCACGCATTCTTAACCAGACAATGCCGCAGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGGC‑GTATGCCGAAGCGGGGCAACCGCAGCATAAAGTGACGGAATTTA‑TTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGG  >  NC_000913/100195‑100457                                                                                                                                      |                                                                                                                                    tgtgCTGGTAGTGGGTGGTTCTCAGGGCGCACGCATTCTTAACCAGACAATGCCGCAGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGAC‑GTATg                                                                                                                              >  1:117606/1‑140 (MQ=255)     cTGGTAGTGGGTGGTTCTCAGGGCGCACGCATTCTTAACCAGACAATGCCGCAGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGACATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGAC‑GTATGCCGa                                                                                                                          >  2:473547/1‑140 (MQ=255)             gggtggTTCTCAGGGCGCACGCATTCTTAACCAGACAATGCCGCAGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGAC‑GTATGCCGAAGCGGGGc                                                                                                                  <  2:117606/140‑1 (MQ=255)              ggtggtTCTCAGGGCGCACGCATTCTTAACCAGACAATGCCGCAGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGAC‑GTATGCCGAAGCGGGGCa                                                                                                                 >  2:14938/1‑140 (MQ=255)               gtggtTCTCAGGGCGCACGCATTCTTAACCAGACAATGCCGCAGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGAC‑GTATGCCGAAGCGGGGCaa                                                                                                                >  2:356969/1‑140 (MQ=255)                tggtTCTCAGGGCGCACGCATTCTTAACCAGACAATGCCGCAGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGAC‑GTATg                                                                                                                              <  2:1094146/125‑1 (MQ=255)                tggtTCTCAGGGCGCACGCATTCTTAACCAGACAATGCCGCAGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGAC‑GTATg                                                                                                                              >  1:1094146/1‑125 (MQ=255)                 ggtTCTCAGGGCGCACGCATTCTTAACCAGACAATGCCGCAGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGAC‑GTATGCCGAAGCGGGGCAAcc                                                                                                              <  1:508353/140‑1 (MQ=255)                  gtTCTCAGGGCGCACGCGTTCTTAACCAGACAATGCCGCAGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGAC‑GTATGCCGAAGCGGGGCAACCg                                                                                                             >  1:207625/1‑140 (MQ=255)                  gtTCTCAGGGCGCACGCATTCTTAACCAGACAATGCCGCAGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGAC‑GTATGCCGAAGCGGGGCAACCg                                                                                                             >  2:1112219/1‑140 (MQ=255)                  gtTCTCAGGGCGCACGCATTCTTAACCAGACAATGCCGCAGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGAC‑GTATGCCGAAGCGGGGCAACCg                                                                                                             >  1:722878/1‑140 (MQ=255)                  gtTCTCAGGGCGCACGCATTCTTAACCAGACAATGCCGCAGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGAC‑GTATGCCGAAGCGGGGCAACCg                                                                                                             >  1:908642/1‑140 (MQ=255)                   ttCTCAGGGCGCACGCATTCTTAACCAGACAATGCCGCAGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGAC‑GTATGCCGAAGCGGGGCAACCgc                                                                                                            >  1:844628/1‑140 (MQ=255)                         gggCGCACGCATTCTTAACCAGACAATGCCGCAGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGAC‑GTATGCCGAAGCGGGGCAACCGCAGCATa                                                                                                      <  2:884619/140‑1 (MQ=255)                              cacgcgTTCTTAACCAGACAATGCCGCAGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGAC‑GTATGCCGAAGCGGGGCAACCGCAGCATAAAGTg                                                                                                 <  2:207625/140‑1 (MQ=255)                              cacgcaTTCTTAACTAGACAATGCCGCAGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGAC‑GTATGCCGAAGCGGGGCAACCGCAGCATAAAGTg                                                                                                 <  2:908642/140‑1 (MQ=255)                                 gcaTTCTTAACCAGACAATGCCGCAGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGAC‑GTATGCCGAAGCGGGGCAACCGCAGCATAAAGTGACg                                                                                              <  1:1112219/140‑1 (MQ=255)                                     tCTTAACCAGACAATGCCGCAGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGAC‑GTATGCCGAAGCGGGGCAACCGCAGCATAAAGTGACGGAAt                                                                                          <  1:1106476/140‑1 (MQ=255)                                        tAACCAGACAATGCCGCAGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGAC‑GTATGCCGAAGCGGGGCAACCGCAGCATAAAGTGACGGAATTTa                                                                                       <  1:839911/140‑1 (MQ=255)                                         aaCCAGACAATGCCGCAGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGAC‑GTATGCCGAAGCGGGGCAACCGCAGCATAAAGTGACGGAATTTA‑t                                                                                     >  1:942045/1‑140 (MQ=255)                                            cAGACAATGCCGCAGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGACATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGAC‑GTATGCCGAAGCGGGGCAACCGCAGCATAAAGTGACGGAATTTA‑Ttga                                                                                  <  1:473547/140‑1 (MQ=255)                                               aCAATGCCGCAGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGAC‑GTATGCCGAAGCGGGGCAACCGCAGCATAAAGTGAc                                                                                               >  2:1167173/1‑125 (MQ=255)                                               aCAATGCCGCAGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGAC‑GTATGCCGAAGCGGGGCAACCGCAGCATAAAGTGAc                                                                                               <  1:1167173/125‑1 (MQ=255)                                                 aaTGCCGCAGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGAC‑GTATGCCGAAGCGGGGCAACCGCAGCATAAAGTGACGGAATTTA‑TTGATGata                                                                             <  2:210042/140‑1 (MQ=255)                                                   tGCCGCAGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGAC‑GTATGCCGAAGCGGGGCAACCGCAGCATAAAGTGACGGAATTTA‑TTGATGATATg                                                                           >  1:157416/1‑140 (MQ=255)                                                    gCCGCAGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGAC‑GTATGCCGAAGCGGGGCAACCGCAGCATAAAGTGACGGAATTTA‑TTGATGATATgg                                                                          >  1:1221880/1‑140 (MQ=255)                                                      cGCAGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGAC‑GTATGCCGAAGCGGGGCAACCGCAGCATAAAGTGACGGAATTTA‑TTGATGATATggcg                                                                        >  1:819572/1‑140 (MQ=255)                                                       gCAGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGAC‑GTATGCCGAAGCGGGGCAACCGCAGCATAAAGTGACGGAATTTA‑TTGATGATATggcgg                                                                       <  2:157416/140‑1 (MQ=255)                                                       gCAGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGAC‑GTATGCCGAAGCGGGGCAACCGCAGCATAAAGTGACGGAATTTA‑TTGATGATATggcgg                                                                       <  2:942045/140‑1 (MQ=255)                                                         aGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGAC‑GTATGCCGAAGCGGGGCAACCGCAGCATAAAGTGACGGAATTTA‑TTGATGATATggcggcg                                                                     <  2:1221880/140‑1 (MQ=255)                                                         aGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGAC‑GTATGCCGAAGCGGGGCAACCGCAGCATAAAGTGACGGAATTTA‑TTGATGATATggcggcg                                                                     <  1:1043901/140‑1 (MQ=255)                                                         aGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGAC‑GTATGCCGAAGCGGGGCAACCGCAGCATAAAGTGACGGAATTTA‑TTGATGATATggcggag                                                                     >  2:996385/1‑138 (MQ=255)                                                           gTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGAC‑GTATGCCGAAGCGGGGCAACCGCAGCATAAAGTGACGGAATTTA‑TTGATGATATggcggcggc                                                                   >  2:242673/1‑140 (MQ=255)                                                                     aaCTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGAC‑GTATGCCGAAGCGGGGCAACCGCAGCATAAAGTGACGGAATTTA‑TTGATGATATGGCGGCGGCGTATGCGTgg                                                         >  1:376577/1‑140 (MQ=255)                                                                       cTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGAC‑GTATGCCGAAGCGGGGCAACCGCAGCATAAAGTGACGGAATTTA‑TTGATGATATGGCGGCGGCGTATGCGTGGGc                                                       <  2:844628/140‑1 (MQ=255)                                                                         gggTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGAC‑GTATGCCGAAGCGGGGCAACCGCAGCATAAAGTGACGGAATTTA‑TTGATGATATGGCGGCGGCGTATGCGTGGGCgg                                                     >  1:33812/1‑140 (MQ=255)                                                                                    tCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGAC‑GTATGCCGAAGCGGGGCAACCGCAGCATAAAGTGACGGAATTTA‑TTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGtt                                          >  2:716147/1‑140 (MQ=255)                                                                                    tCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGAC‑GTATGCCGAAGCGGGGCAACCGCAGCATAAAGTGACGGAATTTA‑TTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGtt                                          >  2:494097/1‑140 (MQ=255)                                                                                    ccACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGAC‑GTATGCCGAAGCGGGGCAACCGCAGCATAAAGTGACGGAATTTA‑TTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGtt                                          >  2:697903/2‑140 (MQ=255)                                                                                        tATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGAC‑GTATGCCGAAGCGGGGCAACCGCAGCATAAAGTGACGGAATTTA‑TTGATGATATGGCGGCGGCGTATGCGTGGGCGGATgt                                                 <  1:907594/129‑1 (MQ=255)                                                                                        tATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGAC‑GTATGCCGAAGCGGGGCAACCGCAGCATAAAGTGACGGAATTTA‑TTGATGATATGGCGGCGGCGTATGCGTGGGCGGATgt                                                 >  2:907594/1‑129 (MQ=255)                                                                                        tATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGAC‑GTATGCCGAAGCGGGGCAACCGCAGCATAAAGTGACGGAATTTA‑TTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGcc                                      <  1:716147/140‑1 (MQ=255)                                                                                        tATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGAC‑GTATGCCGAAGCGGGGCAACCGCAGCATAAAGTGACGGAATTTA‑TTGATGATATGGCGGCGGCGTATGCGTGGGCGGAAGTCGTCGTTTGcc                                      >  2:757556/1‑140 (MQ=255)                                                                                        tATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGAC‑GTATGCCGAAGCGGGGCAACCGCAGCATAAAGTGACGGAATTTA‑TTGATGATATGGCGGCGGCGAATGGGGGGGGGGATGTCGTCGTTTGcc                                      >  2:603897/1‑140 (MQ=255)                                                                                         aTCTGGCATCAGAGCGGCAAAGGTTCGCAGCAATCCGTTGAACAGAC‑GTATGCCGAAGCGGGGCAACCGCAGCATAAAGTGACGGAATTTA‑TTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCg                                     >  2:1075825/1‑140 (MQ=255)                                                                                           cTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGAC‑GTATGCCGAAGCGGGGCAACCGCAGCATAAAGTGACGGAATTTA‑TTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCt                                   <  1:996385/140‑1 (MQ=255)                                                                                              gCATCAGAGCGGCAAGGGTTGGCAACAATCCGTTGAA‑AGGCGGAATGCCAAAGGGGGGCACCCGC‑GGATTAAGGGACGGGATTTATTTGTTGTTTTGCCGGCGGCGAATGGGTGGGGGGATGTCGTCGTTTGCCGCCCCg                                >  2:13709/1‑140 (MQ=255)                                                                                              gCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGAC‑GTATGCCGAAGCGGGGCAACCGCAGCATAAAGTGACGGAATTTA‑TTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCg                                <  1:494097/140‑1 (MQ=255)                                                                                               cATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGAC‑GTATGCCGAAGCGGGGCAACCGCAGCATAAAGTGACGGAATTTA‑TTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCgg                               <  1:757556/140‑1 (MQ=255)                                                                                               cATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGAC‑GTATGCCGAAGCGGGGCAACCGCAGCATAAAGTGACGGAATTTA‑TTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCgg                               <  1:14938/140‑1 (MQ=255)                                                                                                  cAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGAC‑GTATGCCGAAGCGGGGCAACCGCAGCATAAAGTGACGGAATTTA‑TTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGc                            >  2:269598/1‑140 (MQ=255)                                                                                                        ggCAAAGGTTCGCAACAATCCGTTGAACAGAC‑GTATGCCGAAGCGGGGCAACCGCAGCATAAAGTGACGGAATTTA‑TTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAAc                      >  2:280054/1‑140 (MQ=255)                                                                                                          cAAAGGTTCGCAGCAATCCGTTGAACAGAC‑GTATGCCGAAGCGGGGCAACCGCAGCATAAAGTGACGGAATTTA‑TTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACgg                    <  1:1075825/140‑1 (MQ=255)                                                                                                           aaaGGTTCGCAACAATCCGTTGAACAGAC‑GTATGCCGAAGCGGGGCAACCGCAGCATAAAGTGACGGAATTTA‑TTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGgt                   <  1:280054/140‑1 (MQ=255)                                                                                                                    caacaaTCCGTTGAACAGAC‑GTATGCCGAAGCGGGGCAACCGCAGCATAAAGTGACGGAATTTA‑TTGATGATATGGCGGCGGTGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAAt          >  2:1222725/1‑140 (MQ=255)                                                                                                                    caacaaTCCGTTGAACAGAC‑GTATGCCGAAGCGGGGCAACCGCAGCATAAAGTGACGGAATTTA‑TTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAAt          <  1:1251784/140‑1 (MQ=255)                                                                                                                       caaTCCGTTGAACAGAC‑GTATGCCGAAGCGGGGCAACCGCAGCATAAAGTGACGGAATTTA‑TTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATcgc       <  2:722878/140‑1 (MQ=255)                                                                                                                         aTCCGTTGAACAGAC‑GTATGCCGAAGCGGGGCAACCGCAGCATAAAGTGACGGAATTTA‑TTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATcgccg     >  2:337594/1‑140 (MQ=255)                                                                                                                         aTCCGTTGAACAGAC‑GTATGCCGAAGCGGGGCAACCGCAGCATAAAGTGACGGAATTTA‑TTGATGATATGGCGGCGGCGGATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATcgccg     >  1:332065/1‑140 (MQ=255)                                                                                                                            cGTTGAACAGAC‑GTATGCCGAAGCGGGGCAACCGCAGCATAAAGTGACGGAATTTA‑TTGATGATATGGCGGCGGCGGATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCgg  >  1:465738/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                    TGTGCTGGTAGTGGGTGGTTCTCAGGGCGCACGCATTCTTAACCAGACAATGCCGCAGGTTGCTGCGAAACTGGGTGATTCAGTCACTATCTGGCATCAGAGCGGCAAAGGTTCGCAACAATCCGTTGAACAGGC‑GTATGCCGAAGCGGGGCAACCGCAGCATAAAGTGACGGAATTTA‑TTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGG  >  NC_000913/100195‑100457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑