Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,096,209	G→A	V122V (GTG→GTA)	hisF →	imidazole glycerol phosphate synthase subunit HisF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,096,209	0	G	A	100.0%	252.7 / NA	71	V122V (GTG→GTA)	hisF	imidazole glycerol phosphate synthase subunit HisF
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/36);  total (35/36)

TTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGG  >  NC_000913/2096073‑2096336                                                                                                                                         |                                                                                                                                tttGTGTGGCGGGGGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTTCTTTTGCGCGGGTAAAATTTTCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTACAg                                                                                                                              >  1:189416/1‑140 (MQ=255)  ttGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTACAgt                                                                                                                             <  1:769339/140‑1 (MQ=255)   tgtgtgGCGGGTGGGGTTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTACAgtg                                                                                                                            >  2:963125/1‑140 (MQ=255)            gggtggATTTAGTCTCTTGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGt                                                                                                                   <  2:1397939/136‑1 (MQ=255)            ggTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGt                                                                                                                   <  2:506660/140‑1 (MQ=255)             gTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTc                                                                                                                  <  2:1084413/140‑1 (MQ=255)             gTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTc                                                                                                                  <  1:1330566/140‑1 (MQ=255)             gTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTAGCGTACAGTGTATTGTGGTc                                                                                                                  <  2:106110/140‑1 (MQ=255)              tGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCg                                                                                                                 <  2:189416/140‑1 (MQ=255)               gggATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCgg                                                                                                                <  1:132571/140‑1 (MQ=255)                           ctGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTg                                                                                                    >  1:261726/1‑140 (MQ=255)                           ctGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTg                                                                                                    >  2:626589/1‑140 (MQ=255)                            tGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTgg                                                                                                   >  1:390788/1‑140 (MQ=255)                            tGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTgg                                                                                                   >  2:765420/1‑140 (MQ=255)                                aGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTacg                                                                                               <  1:626589/140‑1 (MQ=255)                                 gATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTg                                                                                                           <  2:554502/127‑1 (MQ=255)                                 gATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTg                                                                                                           >  1:554502/1‑127 (MQ=255)                                  aTGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTac                                                                                                <  1:938572/137‑1 (MQ=255)                                  aTGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTac                                                                                                >  2:938572/1‑137 (MQ=255)                                   tGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTacgacg                                                                                            >  2:634813/1‑140 (MQ=255)                                    gCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTg                                                                                                           <  2:445627/124‑1 (MQ=255)                                    gCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTg                                                                                                           >  1:445627/1‑124 (MQ=255)                                     ccGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGcc                                                                                          >  1:1251708/1‑140 (MQ=255)                                       gcgAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGa                                                                                        <  2:1251708/140‑1 (MQ=255)                                        cgAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGaa                                                                                       >  1:424783/1‑140 (MQ=255)                                         gAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGaaa                                                                                      <  1:963125/140‑1 (MQ=255)                                         gAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGaaa                                                                                      <  1:1454061/140‑1 (MQ=255)                                         gAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGaaa                                                                                      <  1:634813/140‑1 (MQ=255)                                                    ccTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAatat                                                                           >  1:1215164/1‑140 (MQ=255)                                                    ccTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAatat                                                                           <  1:323397/140‑1 (MQ=255)                                                       ttGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCAt                                                                        <  2:390788/140‑1 (MQ=255)                                                        tGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCAtg                                                                       <  2:261726/140‑1 (MQ=255)                                                        tGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCAtg                                                                       <  2:1215164/140‑1 (MQ=255)                                                            gcgGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGaa                                                                   <  2:424783/140‑1 (MQ=255)                                                                aTAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAata                                                                            <  1:688653/127‑1 (MQ=255)                                                                aTAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAata                                                                            >  2:688653/1‑127 (MQ=255)                                                                          cATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCg                                                     >  2:1330338/1‑140 (MQ=255)                                                                                ctctCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGaaa                                               >  1:122590/1‑140 (MQ=255)                                                                                      tGCGCTGGCAGACCCAACATTAATTACTCCCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCa                                         >  1:1211451/1‑140 (MQ=255)                                                                                        cgcTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCAcc                                       >  1:1107021/1‑140 (MQ=255)                                                                                        cgcTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCAcc                                       >  1:1467892/1‑140 (MQ=255)                                                                                        cgcTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCAcc                                       >  1:860101/1‑140 (MQ=255)                                                                                        cgcTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCAcc                                       >  1:619321/1‑140 (MQ=255)                                                                                          cTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCcg                                     >  1:491489/1‑140 (MQ=255)                                                                                             gCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGt                                  >  1:710186/1‑140 (MQ=255)                                                                                               aGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCa                                >  1:44125/1‑140 (MQ=255)                                                                                                  cccAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCAc                                        <  1:1383178/129‑1 (MQ=255)                                                                                                  cccAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCAc                                        >  2:1383178/1‑129 (MQ=255)                                                                                                     aaCATTAATTACTCGCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAAt                          >  2:1326358/1‑140 (MQ=255)                                                                                                       cATTAATTACTCGCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATgg                        >  2:1177143/1‑140 (MQ=255)                                                                                                            attaCTCGCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAAc                   >  1:752008/1‑140 (MQ=255)                                                                                                                 tCGCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCa                                <  1:1090712/122‑1 (MQ=255)                                                                                                                 tCGCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCa                                >  2:1090712/1‑122 (MQ=255)                                                                                                                 tCGCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCg              <  2:44125/140‑1 (MQ=255)                                                                                                                  cGCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTAAATGGGAAACGCTCGa             <  1:1442107/140‑1 (MQ=255)                                                                                                                  cGCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTAAATGGGAAACGCTCGa             >  2:1442107/1‑140 (MQ=255)                                                                                                                   gCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGAc            <  2:752008/140‑1 (MQ=255)                                                                                                                   gCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGAc            <  1:316624/140‑1 (MQ=255)                                                                                                                   gCCTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGAc            <  1:210759/140‑1 (MQ=255)                                                                                                                     cTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGAc            >  1:1192850/1‑138 (MQ=255)                                                                                                                     cTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGAc            <  2:1192850/138‑1 (MQ=255)                                                                                                                     cTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTg          <  1:1330338/140‑1 (MQ=255)                                                                                                                     cTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTg          >  1:187320/1‑140 (MQ=255)                                                                                                                     cTGGCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTg          <  2:1107021/140‑1 (MQ=255)                                                                                                                        gCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGAc            <  1:634572/135‑1 (MQ=255)                                                                                                                        gCCGATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGAc            >  2:634572/1‑135 (MQ=255)                                                                                                                           gATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGAc            <  1:586299/132‑1 (MQ=255)                                                                                                                           gATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGAc            >  2:586299/1‑132 (MQ=255)                                                                                                                           gATCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACa    >  2:1399243/1‑140 (MQ=255)                                                                                                                             tCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAgg  >  1:690138/1‑140 (MQ=255)                                                                                                                             tCGCTTTGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAgg  <  2:122590/140‑1 (MQ=255)                                                                                                                                  ttGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGAc            >  2:781599/1‑125 (MQ=255)                                                                                                                                  ttGGCGTACAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGAc            <  1:781599/125‑1 (MQ=255)                                                                                                                                         |                                                                                                                                TTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGG  >  NC_000913/2096073‑2096336

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑