Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,113,672 G→A A219V (GCC→GTC)  galF ← UTP:glucose‑1‑phosphate uridylyltransferase, low activity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,113,6720GA98.7% 276.8 / NA 79A219V (GCC→GTC) galFUTP:glucose‑1‑phosphate uridylyltransferase, low activity
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (38/40);  total (38/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCAGTCGTAACTGTCGCCGGTCATCAGCATTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTTAGTCTGGATGACGGAGTA  >  NC_000913/2113538‑2113808
                                                                                                                                      |                                                                                                                                        
cGCAGTCGTAACTGTCGCCGGTCATCAGCATTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGACAGaa                                                                                                                                     <  2:77669/140‑1 (MQ=255)
   aGTCGTAACTGTCGCCGGTCATCAGCATTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGACAGAAAGc                                                                                                                                  <  2:642304/140‑1 (MQ=255)
      cGTAACTGTCGCCGGTCATCAGCATTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCAAAATATCGACAGAAAGcaca                                                                                                                               <  1:180947/140‑1 (MQ=255)
         aaCTGTCGCCGGTCATCAGCATTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGACAGAAAGCACATAg                                                                                                                            <  2:384265/140‑1 (MQ=255)
           cTGTCGCCGGTCATCAGCATTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGACAGAAAGCACATAGCg                                                                                                                          <  1:793811/140‑1 (MQ=255)
              tCGCCGGTCATCAGCATTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGACAGAAAGCACATAGCGAcc                                                                                                                       >  2:206915/1‑140 (MQ=255)
               cGCCGGTCATCAGCATTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGACAGAAAg                                                                                                                                   >  1:820789/1‑127 (MQ=255)
               cGCCGGTCATCAGCATTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGACAGAAAg                                                                                                                                   <  2:820789/127‑1 (MQ=255)
               cGCCGGTCATCAGCATTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGACAGAAAGCACATAGCTAcc                                                                                                                       >  2:68630/1‑139 (MQ=255)
               cGCCGGTCATCAGCATTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGACAGAAAGCACATAGCTAcc                                                                                                                       <  1:68630/139‑1 (MQ=255)
               cGCCGGTCATCAGCATTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGACAGAAAGCACATAGCGACCt                                                                                                                      >  2:1122395/1‑140 (MQ=255)
                  cGGTCATCAGCATTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGACAGAAAGCACATAGCGACCTACg                                                                                                                   <  1:1312675/140‑1 (MQ=255)
                  cGGTCATCAGCATTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGACAGAAAGCACATAGCGACCTACg                                                                                                                   <  2:875842/140‑1 (MQ=255)
                   ggTCATCAGCATTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCAAAATATCGACAGAAAGCACATAGCGACCTACgg                                                                                                                  >  2:473474/1‑140 (MQ=255)
                       atcaGCATTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGACAGAAAGCACATAGCGACCTACGGCCat                                                                                                              <  1:621829/140‑1 (MQ=255)
                        tcaGCATTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGACAGAAAGCACATAGCGACCTACGGCCatg                                                                                                             <  2:305847/140‑1 (MQ=255)
                         caGCATTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGTCACAAAGTACATAGCGACCTACGGCCatga                                                                                                            <  2:361961/140‑1 (MQ=255)
                             aTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGACAGAAAGCACATAGCGACCTACGGCCATGATGTc                                                                                                        <  1:206915/140‑1 (MQ=255)
                                 cATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAg                                                                                                    >  2:910408/1‑140 (MQ=255)
                                 cATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAg                                                                                                    >  1:649371/1‑140 (MQ=255)
                                   tCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGTCTGAGTACGTTCCAGTTCCGGCCAAATATCGACAGAAAGCACATAGCGACCTACGGCCatga                                                                                                            <  2:1317826/130‑1 (MQ=255)
                                   tCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGACAGAAAGCACATAGCGACCTACGGCCatga                                                                                                            >  1:1317826/1‑130 (MQ=255)
                                   tCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTc                                                                                                  >  2:1264635/1‑140 (MQ=255)
                                     aaCGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCa                                                                                                >  2:1307726/1‑140 (MQ=255)
                                     aaCGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCa                                                                                                <  2:1263467/140‑1 (MQ=255)
                                     aaCGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCa                                                                                                <  2:1180916/140‑1 (MQ=255)
                                      aCGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGACAGAAAGCACATAGCGACCTACGGCCatga                                                                                                            >  1:1278270/1‑127 (MQ=255)
                                      aCGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGACAGAAAGCACATAGCGACCTACGGCCatga                                                                                                            <  2:1278270/127‑1 (MQ=255)
                                                   tCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGAAAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCa                                                                                                <  2:636932/126‑1 (MQ=255)
                                                   tCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGAAAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCa                                                                                                >  1:636932/1‑126 (MQ=255)
                                                    cGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGAt                                                                                 >  1:1459890/1‑140 (MQ=255)
                                                          cTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGtt                                                                           >  1:1418713/1‑140 (MQ=255)
                                                                    gCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCg                                                                      >  2:1374381/1‑135 (MQ=255)
                                                                    gCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCg                                                                      <  1:1374381/135‑1 (MQ=255)
                                                                    gCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATaaa                                                                 >  1:786693/1‑140 (MQ=255)
                                                                    gCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATaaa                                                                 >  2:1357615/1‑140 (MQ=255)
                                                                         agtcagCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTcaa                                                            <  2:1418713/140‑1 (MQ=255)
                                                                            cagCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTcaacaa                                                         <  2:1459890/140‑1 (MQ=255)
                                                                             agCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAAt                                                        >  2:489046/1‑140 (MQ=255)
                                                                             agCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAAt                                                        >  2:1362348/1‑140 (MQ=255)
                                                                                 gAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCgg                                                    <  1:1307726/140‑1 (MQ=255)
                                                                                 gAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCgg                                                    <  2:880013/140‑1 (MQ=255)
                                                                                  aaTACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGc                                                   <  1:1264635/140‑1 (MQ=255)
                                                                                            cATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTAcc                                         >  1:651926/1‑140 (MQ=255)
                                                                                            cATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTAcc                                         >  1:532235/1‑140 (MQ=255)
                                                                                                   cAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACgg                                  >  2:742920/1‑140 (MQ=255)
                                                                                                   cAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACgg                                  >  2:1229755/1‑140 (MQ=255)
                                                                                                     ggCTGAGTACGTTCCAGTTCCGGCCAAATATCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTc                                >  2:1164150/1‑140 (MQ=255)
                                                                                                     ggCTGAGTACGTTCCAGTTCCGGCCAAATATCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTc                                >  2:1068663/1‑140 (MQ=255)
                                                                                                      gCTGAGTACGTTCCAGTTCCGGCCAAATATCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTcc                               >  1:120532/1‑140 (MQ=255)
                                                                                                        tGAGTACGTTCCAGTTCCGGCCAAATATCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAg                             <  2:651926/140‑1 (MQ=255)
                                                                                                          aGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCg                           <  2:824416/140‑1 (MQ=255)
                                                                                                          aGTACGTTCCAGTTCCGGCCAAATATCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCg                           <  2:583521/140‑1 (MQ=255)
                                                                                                          aGTACGTTCCAGTTCCGGCCAAATATCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCg                           >  1:583521/1‑140 (MQ=255)
                                                                                                          aGTACGTTCCAGTTCCGGCCAAATATCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCg                           >  2:888680/1‑140 (MQ=255)
                                                                                                           gTACGTTCCAGTTCCGGCCAAATATCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCgg                          >  1:759281/1‑140 (MQ=255)
                                                                                                               gTTCCAGTTCCGGCCAAATATCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGctct                      >  2:11217/1‑140 (MQ=255)
                                                                                                                ttCCAGTTCCGGCCAAATATCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCtt                     >  2:1201559/1‑140 (MQ=255)
                                                                                                                 tCCAGTTCCGGCCAAATATCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCttt                    >  1:202728/1‑140 (MQ=255)
                                                                                                                  ccAGTTCCGGCCAAATATCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTTa                   <  1:910408/140‑1 (MQ=255)
                                                                                                                    aGTTCCGGCCAAATATCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTTAGt                 <  1:1357615/140‑1 (MQ=255)
                                                                                                                    aGTTCCGGCCAAATATCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTTAGt                 >  1:1019816/1‑140 (MQ=255)
                                                                                                                    aGTTCCGGCCAAATATCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTTAGt                 >  1:1384536/1‑140 (MQ=255)
                                                                                                                      ttCCGGCCAAATATCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTTAGTCt               >  2:100552/1‑140 (MQ=255)
                                                                                                                           gCCAAATATCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTTAGTCTGGATg          >  1:1177940/1‑140 (MQ=255)
                                                                                                                           gCCAAATATCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTTAGTCTGGATg          <  1:100552/140‑1 (MQ=255)
                                                                                                                           gCCAAATATCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTTAGTCTGGATg          >  1:1293320/1‑140 (MQ=255)
                                                                                                                           gCCAAATATCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTTAGTCTGGATg          >  2:1344783/1‑140 (MQ=255)
                                                                                                                           gCCAAATATCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTTAGTCTGGATg          <  2:1019816/140‑1 (MQ=255)
                                                                                                                              aaaTATCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTTAGTCTGGATGACg       <  2:202728/140‑1 (MQ=255)
                                                                                                                               aaTATCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTTAGTCTGGATGACgg      <  1:1229755/140‑1 (MQ=255)
                                                                                                                               aaTATCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTTAGTCTGGATGACgg      >  1:292243/1‑140 (MQ=255)
                                                                                                                               aaTATCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTTAGTCTGGATGACgg      <  1:1164150/140‑1 (MQ=255)
                                                                                                                               aaTATCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTTAGTCTGGATGACgg      <  2:759281/140‑1 (MQ=255)
                                                                                                                               aaTATCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTTAGTCTGGATGACgg      <  1:1122395/140‑1 (MQ=255)
                                                                                                                               aaTATCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTTAGTCTGGATGACgg      <  1:710694/140‑1 (MQ=255)
                                                                                                                               aaTATCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGACCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTTAGTCTGGATGACgg      <  2:1177940/140‑1 (MQ=255)
                                                                                                                                atatCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTTAGTCTGGATGACGGa     <  2:649371/140‑1 (MQ=255)
                                                                                                                                atatCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTTAGTCTGGATGACGGa     <  1:1068663/140‑1 (MQ=255)
                                                                                                                                  atCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTTAGTCTGGATGACGGAGt   <  2:281072/140‑1 (MQ=255)
                                                                                                                                   tCGACAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTTAGTCTGGATGACGGAGTa  <  2:532235/140‑1 (MQ=255)
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CGCAGTCGTAACTGTCGCCGGTCATCAGCATTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGCTGATCCGGTTTTTCGATAAATTCAACAATGCGGCTGACTTTACCCTCACGGTCCAGCGGCTCTTTAGTCTGGATGACGGAGTA  >  NC_000913/2113538‑2113808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑