Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,352,253	T→C	Y41C (TAC→TGC)	glpT ←	sn‑glycerol 3‑phosphate:phosphate antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,352,253	0	T	C	100.0%	249.5 / NA	72	Y41C (TAC→TGC)	glpT	sn‑glycerol 3‑phosphate:phosphate antiporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/32);  total (40/32)

AACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGATCCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAGCCTCCGTG  >  NC_000913/2352122‑2352388                                                                                                                                    |                                                                                                                                        aaCCCATGATGAATTTCGACAATCCATAAGCAATCGAGATCCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGCAAGCCg                                                                                                                                   <  2:1344043/138‑1 (MQ=255) aaCCCATGATGAATTTCGAAAATCCATAAGCAATCGAGATCCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGCAAGCCg                                                                                                                                   >  1:1344043/1‑138 (MQ=255) aaCCCATGATGAATTTCGAAAATCCATAAGCAATCGAGATCCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGCAAGCCGCa                                                                                                                                 >  1:689642/1‑140 (MQ=255) aaCCCATGATGAATTTCGAAAATCCATAAGCAATCGAGATCCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGCAAGCCGCa                                                                                                                                 >  1:1341666/1‑140 (MQ=255) aaCCCATGATGAATTTCGAAAATCCATAAGCAATCGAGATCCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGCAAGCCGCa                                                                                                                                 <  2:47712/140‑1 (MQ=255) aaCCCATGATGAATTTCGAAAATCCATAAGCAATCGAGATCCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGCAAGCCGCa                                                                                                                                 >  1:568820/1‑140 (MQ=255) aaCCCATGATGAATTTCGAAAATCCATAAGCAATCGAGATCCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGCAAGCCGCa                                                                                                                                 <  2:53255/140‑1 (MQ=255) aaCCCATGATGAATTTCGAAAATCCATAAGCAATCGAGATCCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGCAAGCCGCa                                                                                                                                 <  2:689642/140‑1 (MQ=255) aaCCCATGATGAATTTCGAAAATCCATAAGCAATCGAGATCCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGCAAGCCGCa                                                                                                                                 >  1:1098133/1‑140 (MQ=255) aaCCCATGATGAATTTCGAAAATCCATAAGCAATCGAGATCCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGCAAGCCGCa                                                                                                                                 >  1:1084/1‑140 (MQ=255)    ccATGATGAATTTCGAAAATCCATAAGCAATCGAGATCCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGCAAGCCGCATAg                                                                                                                              <  2:1340838/140‑1 (MQ=255)      atgatgAATTTCGAAAATCCATAAGCAATCGAGATCCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGCAAGCCGCATAGcc                                                                                                                            >  2:490283/1‑140 (MQ=255)       tgatgaATTTCGAAAATCCATAAGCAATCGAGATCCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGCAAGCCGCATAGCCa                                                                                                                           <  2:1098133/140‑1 (MQ=255)        gatgaATTTCGAAAATCCATAAGCAATCGAGATCCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGCAAGCCGc                                                                                                                                  >  1:1290341/1‑132 (MQ=255)        gatgaATTTCGAAAATCCATAAGCAATCGAGATCCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGCAAGCCGc                                                                                                                                  <  2:1290341/132‑1 (MQ=255)             aTTTCGAAAATCCATAAGCAATCGAGATCCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGCAAGCCGCATAGCCAAAGAat                                                                                                                     <  1:932345/140‑1 (MQ=255)                                 aTCGAGATCCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCa                                                                                                 >  2:140776/1‑140 (MQ=255)                                 aTCGAGATCCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCa                                                                                                 >  2:502389/1‑140 (MQ=255)                                 aTCGAGATCCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCa                                                                                                 >  2:1183920/1‑140 (MQ=255)                                  tCGAGATCCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAg                                                                                                >  1:847694/1‑140 (MQ=255)                                   cGAGATCCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAgc                                                                                               >  1:991783/1‑140 (MQ=255)                                         ccccGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATc                                                                                         <  2:847694/140‑1 (MQ=255)                                         ccccGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATc                                                                                         <  1:692970/140‑1 (MQ=255)                                            cGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATcgac                                                                                      >  1:1361238/1‑140 (MQ=255)                                                  ggCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGTCAGCGCAATCGACGATAAg                                                                                >  2:259623/1‑140 (MQ=255)                                                             aaTCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGgatcgatc                                                                     >  2:723842/1‑140 (MQ=255)                                                                  ccGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGCAAGCTGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGgat                                                                          >  2:1028609/1‑130 (MQ=255)                                                                  ccGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGgat                                                                          <  1:1028609/130‑1 (MQ=255)                                                                   cgcgTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCcgcg                                                               >  2:216115/1‑140 (MQ=255)                                                                   cgcgTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCcgcg                                                               >  2:357769/1‑140 (MQ=255)                                                                   cgcgTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCcgcg                                                               >  1:515470/1‑140 (MQ=255)                                                                         agaATCCCTGCTCAACCAGATAAGACATAGCAAGCGCAAAGTTCTTACGAGCCAAATAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGt                                                         >  2:78928/1‑140 (MQ=255)                                                                            aTCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAg                                                      <  1:216115/140‑1 (MQ=255)                                                                                 tGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAgcgcgc                                                 >  1:530204/1‑140 (MQ=255)                                                                                  gttCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCt                                                <  2:469088/138‑1 (MQ=255)                                                                                  gCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCt                                                >  1:914762/1‑140 (MQ=255)                                                                                       aCCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTtgtg                                           <  2:991783/140‑1 (MQ=255)                                                                                        ccAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTtgtgt                                          >  2:1005625/1‑140 (MQ=255)                                                                                             tAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGgcgc                                     <  2:515470/140‑1 (MQ=255)                                                                                             tAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGgcgc                                     >  2:782594/1‑140 (MQ=255)                                                                                             tAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGgcgc                                     <  2:159256/140‑1 (MQ=255)                                                                                             tAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGgcgc                                     >  1:1107781/1‑140 (MQ=255)                                                                                                    tAGCAAGCGCAAAGTTCTTACGAACCAAATAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGgcgc                                     >  1:606144/1‑133 (MQ=255)                                                                                                    tAGCAAGCGCAAAGTTCTTACGAACCAAATAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGgcgc                                     <  2:606144/133‑1 (MQ=255)                                                                                                    tAGCAAGCGCAAAGTTCTTACGAACCAAATAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTa                              >  2:1117784/1‑140 (MQ=255)                                                                                                     aGCAAGCGCAAAGTTCTTACGAACCAAATAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTaa                             >  1:1139867/1‑140 (MQ=255)                                                                                                     aGCAAGCGCAAAGTTCTTACGAACCAAATAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTaa                             <  1:78928/140‑1 (MQ=255)                                                                                                      gCAAGCGCAAAGTTCTTACGAACCAAATAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTaaa                            >  2:1021842/1‑140 (MQ=255)                                                                                                       cAAGCGCAAAGTTCTTACGAACCAAATAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGgcgc                                     >  1:961579/1‑130 (MQ=255)                                                                                                       cAAGCGCAAAGTTCTTACGAACCAAATAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGgcgc                                     <  2:961579/130‑1 (MQ=255)                                                                                                       cAAGCGCAAAGTTCTTACGAACCAAATAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTaaaa                           <  1:1117784/140‑1 (MQ=255)                                                                                                          gcgcAAAGTTCTTACGAACCAAATAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGgcgc                                     <  1:768333/127‑1 (MQ=255)                                                                                                          gcgcAAAGTTCTTACGAACCAAATAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGgcgc                                     <  1:1104999/127‑1 (MQ=255)                                                                                                          gcgcAAAGTTCTTACGAACCAAATAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGgcgc                                     >  2:768333/1‑127 (MQ=255)                                                                                                          gcgcAAAGTTCTTACGAACCAAATAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGgcgc                                     >  2:1104999/1‑127 (MQ=255)                                                                                                           cgcAAAGTTCTTACGAACCAAATAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATAc                       <  1:1005625/140‑1 (MQ=255)                                                                                                           cgcAAAGTTCTTACGAACCAAATAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATAc                       >  1:492747/1‑140 (MQ=255)                                                                                                            gcAAAGTTCTTACGAACCAAATAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACt                      <  2:1139867/140‑1 (MQ=255)                                                                                                             cAAAGTTCTTACGAACCAAATAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTc                     <  1:723842/140‑1 (MQ=255)                                                                                                                aGTTCTTACGAACCAAATAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATAc                       >  1:1230598/1‑135 (MQ=255)                                                                                                                aGTTCTTACGAACCAAATAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATAc                       <  2:1230598/135‑1 (MQ=255)                                                                                                                 gTTCTTACGAACCAAATAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACa                 <  2:1107781/140‑1 (MQ=255)                                                                                                                 gTTCTTACGAACCAAATAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGAT‑AGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACAt                >  1:64608/1‑140 (MQ=255)                                                                                                                     ttACGAACCAAATAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGa             <  1:1213664/140‑1 (MQ=255)                                                                                                                        cGAACCAAATAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAg          <  1:1021842/140‑1 (MQ=255)                                                                                                                          aaCCAAATAGCAATCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGGCCTTTTTGTGTCGCTGGTTTAAAAATACTCAACAttagttacc        >  2:1110183/1‑133 (MQ=255)                                                                                                                            ccAAATAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACt                      >  1:628498/1‑124 (MQ=255)                                                                                                                            ccAAATAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACt                      <  2:628498/124‑1 (MQ=255)                                                                                                                            ccAAATAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAGCCTc      >  2:1175615/1‑140 (MQ=255)                                                                                                                            ccAAATAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAGCCTc      >  1:1078973/1‑140 (MQ=255)                                                                                                                            ccAAATAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAGCCTc      <  2:914762/140‑1 (MQ=255)                                                                                                                               aaTAGCAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGAT‑AGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAGCCTCCGTg  <  2:64608/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                        AACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGATCCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAGCCTCCGTG  >  NC_000913/2352122‑2352388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑