Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,396,354	A→G	Y33H (TAC→CAC)	nuoI ←	NADH:quinone oxidoreductase subunit I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,396,354	0	A	G	100.0%	161.3 / NA	48	Y33H (TAC→CAC)	nuoI	NADH:quinone oxidoreductase subunit I
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/21);  total (27/21)

AGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAG  >  NC_000913/2396221‑2396490                                                                                                                                      |                                                                                                                                         aGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTGCAttcg                                                                                                                                    <  2:1150224/140‑1 (MQ=255)                aCCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTGCATTCGCGTTTCGCGTTTGGc                                                                                                                     >  2:1117928/1‑140 (MQ=255)                aCCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACAACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTGCATTCGCGTTTCGCGTTTGGc                                                                                                                     >  2:1430999/1‑140 (MQ=255)                  cGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTGCATTCGCGTTTCGCGTTTGGCGa                                                                                                                   >  1:867604/1‑140 (MQ=255)                   gggCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTGCATTCGCGTTTCGCGTTTGGCGaa                                                                                                                  <  2:391373/140‑1 (MQ=255)                    ggCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGTTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTGCATTCGCGTTTCGCGTTTGGCGAAc                                                                                                                 >  1:1121106/1‑140 (MQ=255)                    ggCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTGCATTCGCGTTTCGCGTTTGGCGAAc                                                                                                                 <  2:1172764/140‑1 (MQ=255)                              cgcgCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTGCATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGc                                                                                                       >  1:855699/1‑140 (MQ=255)                               gcgcAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTGCATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGcc                                                                                                      >  1:303426/1‑140 (MQ=255)                                cgcAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTGCATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCg                                                                                                     <  1:1060541/140‑1 (MQ=255)                                  cAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGTTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTGCATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGat                                                                                                   <  2:1121106/140‑1 (MQ=255)                                  cAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTGCATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGat                                                                                                   <  2:855699/140‑1 (MQ=255)                                  cAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTGCATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGat                                                                                                   >  1:605477/1‑140 (MQ=255)                                   agagGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTGCATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGatc                                                                                                  >  2:166309/1‑140 (MQ=255)                                   agagGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTGCATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGatc                                                                                                  >  2:754247/1‑140 (MQ=255)                                          aCAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCTCGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTGCATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGa                                                                                           <  2:743701/140‑1 (MQ=255)                                          aCAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTGCATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGa                                                                                           <  2:351516/140‑1 (MQ=255)                                                 aCGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTGCATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACg                                                                                    >  1:619485/1‑140 (MQ=255)                                                       cGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTGCATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTg                                                                              <  2:303426/140‑1 (MQ=255)                                                        gCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTGCATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTgg                                                                             >  1:1159393/1‑140 (MQ=255)                                                        gCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTGCATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTgg                                                                             >  2:1412823/1‑140 (MQ=255)                                                           ctTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTGCATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTg                                                                          <  1:1471810/140‑1 (MQ=255)                                                            ttCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTGCATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGc                                                                         >  2:1173427/1‑140 (MQ=255)                                                              cGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTGCATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCg                                                                       >  1:97599/1‑140 (MQ=255)                                                               gCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTGCATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGa                                                                      <  2:97599/140‑1 (MQ=255)                                                               gCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTGCATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGa                                                                      <  1:166309/140‑1 (MQ=255)                                                                 cGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTGCATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGaaa                                                                    <  2:488399/140‑1 (MQ=255)                                                                    ccGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGGCCGGCTCTTCCGGGTGCATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACct                                                                 >  2:1348171/1‑140 (MQ=255)                                                                       ggTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTGCATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACctact                                                              <  2:1340712/140‑1 (MQ=255)                                                                             gggTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTGCATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAAt                                                        >  2:1181144/1‑140 (MQ=255)                                                                                    aaCGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTGCATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTACCGAAACCTACTAACAATTCTTTTa                                                 <  2:867604/140‑1 (MQ=255)                                                                                         tACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTGCATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTc                                            >  1:1434694/1‑140 (MQ=255)                                                                                              ccACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTGCATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGt                                       >  2:997560/1‑140 (MQ=255)                                                                                                  gATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTGCATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTa                                   <  1:1117928/140‑1 (MQ=255)                                                                                                       cGGGGCGGCAGATAGACCGGCTCTTCCGGGTGCATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGcc                              <  1:637633/140‑1 (MQ=255)                                                                                                            cggcAGATAGACCGGCTCTTCCGGGTGCATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGTCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTa                         >  2:1225733/1‑140 (MQ=255)                                                                                                            cggcAGATAGACCGGCTCTTCCGGGTGCATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTa                         >  1:941716/1‑140 (MQ=255)                                                                                                            cggcAGATAGACCGGCTCTTCCGGGTGCATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTa                         >  1:539445/1‑140 (MQ=255)                                                                                                            cggcAGATAGACCGGCTCTTCCGGGTGCATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATAATACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTa                         <  1:1430999/140‑1 (MQ=255)                                                                                                               cAGATAGACCGGCTCTTCCGGGTGCATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTg                      >  1:783887/1‑140 (MQ=255)                                                                                                                 gATAGACCGGCTCTTCCGGGTGCATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTgcg                    <  2:1159393/140‑1 (MQ=255)                                                                                                                  aTAGACCGGCTCTTCCGGGTGCATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTgcgc                   <  2:539445/140‑1 (MQ=255)                                                                                                                      gccGGCTCTTCCGGGTGCATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGc               <  1:1348171/139‑1 (MQ=255)                                                                                                                          gCTCTTCCGGGTGCATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCaga           >  2:1027871/1‑140 (MQ=255)                                                                                                                           ctctTCCGGGTGCATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCagag          >  2:1375728/1‑140 (MQ=255)                                                                                                                           ctctTCCGGGTGCATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCagag          >  2:904457/1‑140 (MQ=255)                                                                                                                            tctTCCGGGTGCATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAgaga         >  2:280373/1‑140 (MQ=255)                                                                                                                                   ggTGCATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAg  >  2:792081/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                         AGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAG  >  NC_000913/2396221‑2396490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑