Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,673,991	A→G	F233S (TTC→TCC)	yphB ←	putative aldose 1‑epimerase YphB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,673,991	0	A	G	100.0%	209.4 / NA	61	F233S (TTC→TCC)	yphB	putative aldose 1‑epimerase YphB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/28);  total (33/28)

TTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCCAGACAGAAGAAATCAAACGCATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGAAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTCCTGCGGCTGCTCGATGCGGGCCTGACCATTCCATCCGGCAAAACCATTGTTCACCCACTGGCGCGGCAACGGCGCGGGCTGGTTAA  >  NC_000913/2673854‑2674126                                                                                                                                          |                                                                                                                                        ttgATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCCAGACAGAAGAAATCAAACGCATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGgaa                                                                                                                                       >  2:1391116/1‑140 (MQ=255) ttgATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCCAGACAGAAGAAATCAAACGCATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGgaa                                                                                                                                       <  1:940460/140‑1 (MQ=255)         ccTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCCAGACAGAAGAAATCAAACGCATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGGAATAACACgg                                                                                                                               >  1:1052530/1‑140 (MQ=255)           tGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCCAGACAGAAGAAATCAAACGCATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGGAATAACACGGTg                                                                                                                             >  1:1297402/1‑140 (MQ=255)                  aGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCCAGACAGAAGAAATCAAACGCATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGgaa                                                                                                                                       >  2:562157/1‑123 (MQ=255)                  aGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCCAGACAGAAGAAATCAAACGCATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGGAATAaca                                                                                                                                  >  2:357496/1‑128 (MQ=255)                  aGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCCAGACAGAAGAAATCAAACGCATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGGAATAaca                                                                                                                                  <  1:357496/128‑1 (MQ=255)                  aGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCCAGACAGAAGAAATCAAACGCATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGGAATAACACGGTGCAGGTgg                                                                                                                      >  1:675485/1‑140 (MQ=255)                      cAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCCAGACAGAAGAAATCAAACGCATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGGAATAACACGGTGCAGGTGGcgtc                                                                                                                  <  1:870722/140‑1 (MQ=255)                       aaTGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCCAGACAGAAGAAATCAAACGCATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGGAATAACACGGTGCAGGTGGcgtcg                                                                                                                 <  1:768619/140‑1 (MQ=255)                              tCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCCAGACAGAAGAAATCAAACGCATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGGAATAACACGGTGCAGGTGGCGTCGTTTCCat                                                                                                          >  1:1414969/1‑140 (MQ=255)                                  ccccTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCCAGACAGAAGAAATCAAACGCATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGGAATAACACGGTGCAGGTGGCGTCGTTTCCatgatg                                                                                                      >  1:799514/1‑140 (MQ=255)                                  ccccTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCCAGACAGAAGAAATCAAACGCATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGGAATAACACGGTGCAGGTGGCGTCGTTTCCatgatg                                                                                                      <  1:1391116/140‑1 (MQ=255)                                      ttCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCCAGACAGAAGAAATCAAACGCATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGGAATAACACGGTGCAGGTGGcgtcg                                                                                                                 >  1:514984/1‑125 (MQ=255)                                      ttCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCCAGACAGAAGAAATCAAACGCATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGGAATAACACGGTGCAGGTGGcgtcg                                                                                                                 <  2:514984/125‑1 (MQ=255)                                      ttCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCCAGACAGAAGAAATCAAACGCATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGGAATAACACGGTGCAGGTGGCGTCGTTTCCatgatg                                                                                                      <  2:421688/136‑1 (MQ=255)                                      ttCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCCAGACAGAAGAAATCAAACGCATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGGAATAACACGGTGCAGGTGGCGTCGTTTCCatgatg                                                                                                      >  1:421688/1‑136 (MQ=255)                                      ttCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCCAGACAGAAGAAATCAAACGCATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGGAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATgg                                                                                                  <  2:1297402/140‑1 (MQ=255)                                       tCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCCAGACAGAAGAAATCAAACGCATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGGAATAACACGGTGCAGGTGGCGTCGTTTcc                                                                                                            <  1:352572/129‑1 (MQ=255)                                       tCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCCAGACAGAAGAAATCAAACGCATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGGAATAACACGGTGCAGGTGGCGTCGTTTcc                                                                                                            >  2:352572/1‑129 (MQ=255)                                           ggCGATGATGATCATCCGGCGCATGGCTCATCGGTTCCAGACAGAAGAAATCAAACGCATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGGAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATaa                                                                                             >  1:754601/1‑140 (MQ=255)                                            gCGATGATGATCATCCGGCGCATGGCTCATCGGTTCCAGACAGAAGAAATCAAACGCATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGGAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATacc                                                                                            >  1:1397708/1‑138 (MQ=255)                                            gCGATGATGATCATCCGGCGCATGGCTCATCGGTTCCAGACAGAAGAAATCAAACGCATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGGAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATAAc                                                                                            <  2:484745/140‑1 (MQ=255)                                                 gatgatCATCCGGCGCATGGCTCATCGGTTCCAGACAGAAGAAATCAAACGCATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGGAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTcc                                                                                       <  2:776494/140‑1 (MQ=255)                                                     atcatcCGGCGCATGGCTCATCGGTTCCAGACAGAAGAAATCAAACGCATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGGAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTCCTGCg                                                                                   >  2:1157745/1‑140 (MQ=255)                                                      tcatcCGGCGCATGGCTCATCGGTTCCAGACAGAAGAAATCAAACGCATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGGAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTCCTGCgg                                                                                  >  2:676000/1‑140 (MQ=255)                                                           cGGCGCATGGCTCATCGGTTCCAGACAGAAGAAATCAAACGCATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGGAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTCCTGCGgctgct                                                                             <  1:1157745/140‑1 (MQ=255)                                                           cGGCGCATGGCTCATCGGTTCCAGACAGAAGAAATCAAACGCATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGGAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTCCTGCGgctgct                                                                             <  2:675485/140‑1 (MQ=255)                                                            ggCGCATGGCTCATCGGTTCCAGACAGAAGAAATCAAACGCATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGGAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTCCTGCGGCTGCTc                                                                            >  2:357818/1‑140 (MQ=255)                                                                cATGGCTCATCGGTTCCAGACAGAAGAAATCAAACGCATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGGAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTCCTGCGGCTGCTCGATg                                                                        <  1:357818/140‑1 (MQ=255)                                                                cATGGCTCATCGGTTCCAGACAGAAGAAATCAAACGCATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGGAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTCCTGCGGCTGCTCGATg                                                                        <  1:676000/140‑1 (MQ=255)                                                                    gCTCATCGGTTCCAGACAGAAGAAATCAAACGCATATCCTTTATCAAACGCAGTGTCGGAAACAAAGATGGAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTCCTGCGGCTGCTCGATGCggg                                                                    <  2:1223918/140‑1 (MQ=255)                                                                    gCTCATCGGTTCCAGACAGAAGAAATCAAACGCATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGGAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTCCTGCGGCTGCTCGATGCggg                                                                    <  2:754601/140‑1 (MQ=255)                                                                        aTCGGTTCCAGACAGAAGAAATCAAACGCATATCCTTTATTAAACGCAGGGTCGGAAACAAAGATGGAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTCCTGCGGCTGCTCGATGCGGGCCTg                                                                >  2:563460/1‑140 (MQ=255)                                                                        aTCGGTTCCAGACAGAAGAAATCAAACGCATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGGAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTCCTGCGGCTGCTCGATGCGGGCCTg                                                                >  1:1439812/1‑140 (MQ=255)                                                                        aTCGGTTCCAGACAGAAGAAATCAAACGCATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGGAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTCCTGCGGCTGCTCGATGCGGGCCTg                                                                <  2:1439812/140‑1 (MQ=255)                                                                        aTCGGTTCCAGACAGAAGAAATCAAACGCATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGGAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTCCTGCGGCTGCTCGATGCGGGCCTg                                                                >  1:125305/1‑140 (MQ=255)                                                                             ttCCAGACAGAAGAAATCAAACGCATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGGAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTCCTGCGGCTGCTCGATGCGGGCCTGACCAt                                                           >  2:1386130/1‑140 (MQ=255)                                                                                  gacagaAGAAATCAAACGCATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGGAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTCCTGCGGCTGCTCGATGCGGGCCTGACCATTCCAt                                                      >  1:532316/1‑140 (MQ=255)                                                                                      gaagaaATCAAACGCATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGGAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTCCTGCGGCTGCTCGATGCGGGCCTGACCATTCCATCCgg                                                  <  1:1386130/140‑1 (MQ=255)                                                                                      gaagaaATCAAACGCATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGGAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTCCTGCGGCTGCTCGATGCGGGCCTGACCATTCCATCCgg                                                  <  2:1414969/140‑1 (MQ=255)                                                                                       aagaaATCAAACGCATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGGAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTCCTGCGGCTGCTCGATGCGGGCCTGACCATTCCa                                                       <  2:818738/134‑1 (MQ=255)                                                                                       aagaaATCAAACGCATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGGAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTCCTGCGGCTGCTCGATGCGGGCCTGACCATTCCa                                                       >  1:818738/1‑134 (MQ=255)                                                                                       aagaaATCAAACGCATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGGAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTCCTGCGGCTGCTCGATGCGGGCCTGACCATTCCATCCGGc                                                 >  1:1396828/1‑140 (MQ=255)                                                                                        agaaATCAAACGCATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGGAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTCCTGCGGCTGCTCGATGCGGGCCTGACCATTCCATCCGGCa                                                >  1:929941/1‑140 (MQ=255)                                                                                            aTCAAACGCATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGGAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTCCTGCGGCTGCTCGATGCGGGCCTGACCATTCCATCCGGCAAAAc                                            >  2:27052/1‑140 (MQ=255)                                                                                                        tCCTTTATCAAACGCAGGGTCGGAAACAAAGATGGAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTCCTGCGGCTGCTCGATGCGGGCCTGACCATTCCATCCGGCAAAACCATTGTTCAccc                                <  1:27052/140‑1 (MQ=255)                                                                                                                   aCGCAGGGTCGGAAACAAAGATGGAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTCCTGCGGCTGCTCGATGCGGGCCTGACCATTCCATCCGGCAAAACCATTGTTCACCCACTGGCGCGGc                     >  2:593162/1‑140 (MQ=255)                                                                                                                   aCGCAGGGTCGGAAACAAAGATGGAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTCCTGCGGCTGCTCGATGCGGGCCTGACCATTCCATCCGGCAAAACCATTGTTCACCCACTGGCGCGGc                     >  2:814548/1‑140 (MQ=255)                                                                                                                   aCGCAGGGTCGGAAACAAAGATGGAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTCCTGCGGCTGCTCGATGCGGGCCTGACCATTCCATCCGGCAAAACCATTGTTCACCCACTGGCGCGGc                     >  2:180561/1‑140 (MQ=255)                                                                                                                    cGCAGGGTCGGAAACAAAGATGGAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTCCTGCGGCTGCTCGATGCGGGCCTGACCATTCCATCCGGCAAAACCATTGt                                      >  1:655540/1‑122 (MQ=255)                                                                                                                    cGCAGGGTCGGAAACAAAGATGGAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTCCTGCGGCTGCTCGATGCGGGCCTGACCATTCCATCCGGCAAAACCATTGt                                      <  2:655540/122‑1 (MQ=255)                                                                                                                    cGCAGGGTCGGAAACAAAGATGGAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTCCTGCGGCTGCTCGATGCGGGCCTGACCATTCCATCCGGCAAAACCATTGTTCACCCACTGGCGCGGCa                    <  2:592675/140‑1 (MQ=255)                                                                                                                    cGCAGGGTCGGAAACAAAGATGGAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTCCTGCGGCTGCTCGATGCGGGCCTGACCATTCCATCCGGCAAAACCATTGTTCACCCACTGGCGCGGCa                    >  1:592675/1‑140 (MQ=255)                                                                                                                         ggTCGGAAACAAAGATGGAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTCCTGCGGCTGCTCGATGCGGGCCTGACCATTCCATCCGGCAAAACCATTGTTCACCCACTGGCGCGGCAACGgc               >  1:1074096/1‑140 (MQ=255)                                                                                                                         ggTCGGAAACAAAGATGGAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTCCTGCGGCTGCTCGATGCGGGCCTGACCATTCCATCCGGCAAAACCATTGTTCACCCACTGGCGCGGCAACGgc               >  2:670440/1‑140 (MQ=255)                                                                                                                         ggTCGGAAACAAAGATGGAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTCCTGCGGCTGCTCGATGCGGGCCTGACCATTCCATCCGGCAAAACCATTGTTCACCCACTGGCGCGGCAACGgc               >  1:1159575/1‑140 (MQ=255)                                                                                                                          gTCGGAAACAAAGATGGAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTCCTGCGGCTGCTCGATGCGGGCCTGACCATTCCATCCGGCAAAACCATTGTTCACCCACTGGCGCGGCAACGgcg              <  2:125305/140‑1 (MQ=255)                                                                                                                             ggAAACAAAGATGGAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTCCTGCGGCTGCTCGATGCGGGCCTGACCATTCCATCCGGCAAAACCATTGTTCACCCACTGGCGCGGCAACGGCGCgg           <  2:929941/140‑1 (MQ=255)                                                                                                                             ggAAACAAAGATGGAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTCCTGCGGCTGCTCGATGCGGGCCTGACCATTCCATCCGGCAAAACCATTGTTCACCCACTGGCGCGACAACGGCGCgg           >  1:290894/1‑140 (MQ=255)                                                                                                                              gAAACAAAGATGGAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTCCTGCGGCTGCTCGATGCGGGCCTGACCATTCCATCCGGCAAAACCATTGTTCACCCACTGGCGCGGCAACGGCGCggg          >  1:865354/1‑140 (MQ=255)                                                                                                                              gAAACAAAGATGGAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTCCTGCGGCTGCTCGATGCGGGCCTGACCATTCCATCCGGCAAAACCATTGTTCACCCACTGGCGCGGCAACGGCGCggg          <  1:814548/140‑1 (MQ=255)                                                                                                                              gAAACAAAGATGGAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTCCTGCGGCTGCTCGATGCGGGCCTGACCATTCCATCCGGCAAAACCATTGTTCACCCACTGGCGCGGCAACGGCGCggg          <  2:865354/140‑1 (MQ=255)                                                                                                                                      gATGGAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTCCTGCGGCTGCTCGATGCGGGCCTGACCATTCCATCCGGCAAAACCATTGTTCACCCACTGGCGCGGCAACGGCGCGGGCTGGTTaa  <  1:670440/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        TTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCCAGACAGAAGAAATCAAACGCATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGAAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTCCTGCGGCTGCTCGATGCGGGCCTGACCATTCCATCCGGCAAAACCATTGTTCACCCACTGGCGCGGCAACGGCGCGGGCTGGTTAA  >  NC_000913/2673854‑2674126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑