Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,678,695	T→C	M225V (ATG→GTG)	yphF ←	putative ABC transporter periplasmic binding protein YphF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,678,695	0	T	C	100.0%	168.6 / NA	49	M225V (ATG→GTG)	yphF	putative ABC transporter periplasmic binding protein YphF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/29);  total (20/29)

CAGCACCTGATTGTTTTCCAGCTCCTGAGCAATTTCGGTTGTCATATCCGAACCGAAAACAGCAATTTTTCCGGCCTGATTTTGATTACGTACCGCTTTTACCGCGCCGAGTGTCGCACCGCCCGATTCCCCCATAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACTTCTTCAAA  >  NC_000913/2678561‑2678824                                                                                                                                       |                                                                                                                                  cAGCACCTGATTGTTTTCCAGCTCCTGAGCAATTTCGGTTGTCATATCCGAACCGAAAACAGCAATTTTTCCGGCCTGATTTTGATTACGTACCGCTTTTACCGCGCCGAGTGTCGCACCGCCCGATTCCCCCACAATgg                                                                                                                              <  1:932742/140‑1 (MQ=255) cAGCACCTGATTGTTTTCCAGCTCCTGAGCAATTTCGGTTGTCATATCCGAACCGAAAACAGCAATTTTTCCGGCCTGATTTTGATTACGTACCGCTTTTACCGCGCCGAGTGTCGCACCGCCCGATTCCCCCACAATgg                                                                                                                              <  1:759648/140‑1 (MQ=255)    cACCTGATTGTTTTCCAGCTCCTGAGCAATTTCGGTTGTCATATCCGAACCGAAAACAGCAATTTTTCCGGCCTGATTTTGATTACGTACCGCTTTTACCGCGCCGAGTGTCGCACCGCCCGATTCCCCCACAATGGCGt                                                                                                                           >  2:1267314/1‑140 (MQ=255)          aTTGTTTTCCAGCTCCTGAGCAATTTCGGTTGTCATATCCGAACCGAAAACAGCAATTTTTCCGGCCTGATTTTGATTACGTACCGCTTTTACCGCGCCGAGTGTCGCACCGCCCGATTCCCCCACAATGGCGTTGAGAt                                                                                                                     <  2:80789/140‑1 (MQ=255)          aTTGTTTTCCAGCTCCTGAGCAATTTCGGTTGTCATATCCGAACCGAAAACAGCAATTTTTCCGGCCTGATTTTGATTACGTACCGCTTTTACCGCGCCGAGTGTCGCACCGCCCGATTCCCCCACAATGGCGTTGAGAt                                                                                                                     >  2:107217/1‑140 (MQ=255)          aTTGTTTTCCAGCTCCTGAGCAATTTCGGTTGTCATATCCGAACCGAAAACAGCAATTTTTCCGGCCTGATTTTGATTACGTACCGCTTTTACCGCGCCGAGTGTCGCACCGCCCGATTCCCCCACAATGGCGTTGAGAt                                                                                                                     <  1:1299604/140‑1 (MQ=255)                ttCCAGCTCCTGAGCAATTTCGGTTGTCATATCCGAACCGAAAACAGCAATTTTTCCGGCCTGATTTTGATTACGTACCGCTTTTACCGCGCCGAGTGTCGCACCGCCCGATTCCCCCACAATGGCGTTGAGATCCGGCg                                                                                                               >  1:218070/1‑140 (MQ=255)                 tCCAGCTCCTGAGCAATTTCGGTTGTCATATCCGAACCGAAAACAGCAATTTTTCCGGCCTGATTTTGATTACGTACCGCTTTTACCGCGCCGAGTGTCGCACCGCCCGATTCCCCCACAATGGCGTTGAGATCCGGCGt                                                                                                              >  2:806464/1‑140 (MQ=255)                   cAGCTCCTGAGCAATTTCGGTTGTCATATCCGAACCGAAAACAGCAATTTTTCCGGCCTGATTTTGATTACGTACCGCTTTTACCGCGCCGAGTGTCGCATCGCCCGATTCCCCCACAATGGCGTTGAGATCCGGCGTgg                                                                                                            >  1:667910/1‑140 (MQ=255)                   cAGCTCCTGAGCAATTTCGGTTGTCATATCCGAACCGAAAACAGCAATTTTTCCGGCCTGATTTTGATTACGTACCGCTTTTACCGCGCCGAGTGTCGCACCGCCCGATTCCCCCACAATGGCGTTGAGATCCGGCGTgg                                                                                                            <  2:693848/140‑1 (MQ=255)                         cTGAGCAATTTCGGTTGTCATATCCGAACCGAAAACAGCAATTTTTCCGGCCTGATTTTGATTACGTACCGCTTTTACCGCGCCGAGTGTTGCACCGCCCGATTCCCCCACAATGGCGTTGAGATCCGGCGTGGAGATaa                                                                                                      >  1:1466646/1‑140 (MQ=255)                          tGAGCAATTTCGGTTGTCATATCCGAACCGAAAACAGCAATTTTTCCGGCCTGATTTTGATTACGTACCGCTTTTACCGCGCCGAGTGTCGCACCGCCCGATTCCCCCACAATGGCGTTGAGATCCGGCGTGGAGATAAt                                                                                                     <  1:326406/140‑1 (MQ=255)                           gAGCAATTTCGGTTGTCATATCCGAACCGAAAACAGCAATTTTTCCGGCCTGATTTTGATTACGTACCGCTTTTACCGCGCCGAGTGTCGCACCGCCCGATTCCCCCACAATGGCGTTGAGATCCGGCGTGGAGATAAtt                                                                                                    <  1:1267314/140‑1 (MQ=255)                                  ttCGGTTGTCATATCCGAACCGAAAACAGCAATTTTTCCGGCATGATTTTGATTACGTACCGCTTTTACCGCGCCGAGTGTCGCACCGCCCGATTCCCCCACAATGGCGTTGAGATCCGGCGTGGAGATAATTAGttttt                                                                                             <  2:759300/140‑1 (MQ=255)                                      gTTGTCATATCCGAACCGAAAACAGCAATTTTTCCGGCCTGATTTTGATTACGTACCGCTTTTACCGCGCCGAGTGTCGCACCGCCCGATTCCCCCACAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCAcc                                                                                         >  1:377435/1‑140 (MQ=255)                                         gTCATATCCGAACCGAAAACAGCAATTTTTCCGGCCTGATTTTGATTACGTACCGCTTTTACCGCGCCGAGTGTCGCACCGCCCGATTCCCCCACAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAAc                                                                                      >  1:638293/1‑140 (MQ=255)                                           cATATCCGAACCGAAAACAGCAATTTTTCCGGCCTGATTTTGATTACGTACCGCTTTTACCGCGCCGAGTGTCGCACCGCCCGATTCCCCCACAATGGCGTTGAGATCCGGCGTGGAGATAATTAGttt                                                                                               <  2:1171881/129‑1 (MQ=255)                                           cATATCCGAACCGAAAACAGCAATTTTTCCGGCCTGATTTTGATTACGTACCGCTTTTACCGCGCCGAGTGTCGCACCGCCCGATTCCCCCACAATGGCGTTGAGATCCGGCGTGGAGATAATTAGttt                                                                                               >  1:1171881/1‑129 (MQ=255)                                                   aaccgaaAACAGCAATTTTTCCGGCCTGATTTTGATTACGTACCGCTTTTACCGCGCCGAGTGTCGCACCGCCCGATTCCCCCACAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCt                                                                            <  2:218070/140‑1 (MQ=255)                                                   aaccgaaAACAGCAATTTTTCCGGCCTGATTTTGATTACGTACCGCTTTTACCGCGCCGAGTGTCGCACCGCCCGATTCCCCCACAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCt                                                                            <  1:839033/140‑1 (MQ=255)                                                    accgaaAACAGCAATTTTTCCGGCCTGATTTTGATTACGTACCGCTTTTACCGCGCCGAGTGTCGCACCGCCCGATTCCCCCACAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCtt                                                                           >  2:923811/1‑140 (MQ=255)                                                    accgaaAACAGCAATTTTTCCGGCCTGATTTTGATTACGTACCGCTTTTACCGCGCCGAGTGTCGCACCGCCCGATTCCCCCACAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCtt                                                                           >  2:1434281/1‑140 (MQ=255)                                                    accgaaAACAGCAATTTTTCCGGCCTGATTTTGATTACGTACCGCTTTTACCGCGCCGAGTGTCGCACCGCCCGATTCCCCCACAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCtt                                                                           >  2:506459/1‑140 (MQ=255)                                                    accgaaAACAGCAATTTTTCCGGCCTGATTTTGATTACGTACCGCTTTTACCGCGCCGAGTGTCGCACCGCCCGATTCCCCCACAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCtt                                                                           >  1:508825/1‑140 (MQ=255)                                                    accgaaAACAGCAATTTTTCCGGCCTGATTTTGATTACGTACCGCTTTTACCGCGCCGAGTGTCGCACCGCCCGATTCCCCCACAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCt                                                                            <  1:1468747/139‑1 (MQ=255)                                                    accgaaAACAGCAATTTTTCCGGCCTGATTTTGATTACGTACCGCTTTTACCGCGCCGAGTGTCGCACCGCCCGATTCCCCCACAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCt                                                                            >  2:1468747/1‑139 (MQ=255)                                                    accgaaAACAGCAATTTTTCCGGCCTGATTTTGATCACGTACCGCTTTTACCGCGCCGAGTGTCGCACCGCCCGATTCCCCCACAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCtt                                                                           >  1:1273232/1‑140 (MQ=255)                                                        aaaaCAGCAATTTTTCCGGCCTGATTTTGATTACGTACCGCTTTTACCGCGCCGAGTGTCGCACCGCCCGATTCCCCCACAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATc                                                                       <  1:1120998/140‑1 (MQ=255)                                                        aaaaCAGCAATTTTTCCGGCCTGATTTTGATTACGTACCGCTTTTACCGCGCCGAGTGTCGCACCGCCCGATTCCCCCACAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATc                                                                       >  2:1402249/1‑140 (MQ=255)                                                            gaGCAATTTTTCCGGCCTGATTTTGATTACCTGCCGCTTTTACCGCGCCGAGTGTCGCATCGCCCGATTCCCCCTCAGTGGCGTTGAGATCCGGCGTGGAGATAAGTAGTTTTTCACCAACGGAAATCGCTTTATCTaaa                                                                   <  2:365412/139‑1 (MQ=255)                                                             aGCAATTTTTCCGGCCTGATTTTGATTACGTACCGCTTTTACCGCGCCGAGTGTCGCACCGCCCGATTCCCCCACAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTaaaa                                                                  <  2:508825/140‑1 (MQ=255)                                                                  tttttCCGGCCTGATTTTGATTACGTACCGCTTTTACCGCGCCGAGTGTTGCACCGCCCGATTCCCCCACAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCATCAACGGAAATCGCTTTATCTAAAACAGTc                                                             <  2:1466646/140‑1 (MQ=255)                                                                  tttttCCGGCCTGATTTTGATTACGTACCGCTTTTACCGCGCCGAGTGTCGCATCGCCCGATTCCCCCACAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTc                                                             <  2:667910/140‑1 (MQ=255)                                                                  tttttCCGGCCTGATTTTGATTACGTACCGCTTTTACCGCGCCGAGTGTCGCACCGCCCGATTCCCCCACAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTc                                                             <  1:506459/140‑1 (MQ=255)                                                                       ccGGCCTGATTTTGATTACGTACCGCTTTTACCGCGCCGAGTGTCGCACCGCCCGATTCCCCCACAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTc                                                        <  2:231453/140‑1 (MQ=255)                                                                        cGGCCTGATTTTGATTACGTACCGCTTTTACCGCGCCGAGTGTCGCACCGCCCGATTCCCCCACAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTcc                                                       <  2:377435/140‑1 (MQ=255)                                                                            cTGATTTTGATTACGTACCGCTTTTACCGCGCCGAGTGTCGCACCGCCCGATTCCCCCACAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGAt                                                   <  1:923811/140‑1 (MQ=255)                                                                                         cGTACCGCTTTTACCGCGCCGAGTGTCGCACCGCCCGATTCCCCCACAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTg                                      >  1:247543/1‑140 (MQ=255)                                                                                         cGTACCGCTTTTACCGCGCCGAGTGTCGCACCGCCCGATTCCCCCACAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTg                                      >  1:13653/1‑140 (MQ=255)                                                                                              cGCTTTTACCGCGCCGAGTGTCGCACCGCCCGATTCCCCCACAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTgcgcgc                                 >  2:447116/1‑140 (MQ=255)                                                                                                 ttttACCGCGCCGAGTGTCGCACCGCCCGATTCCCCCACAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCgg                              <  1:415100/140‑1 (MQ=255)                                                                                                        gcgcCGAGTGTCGCACCGCCCGATTCCCCCACAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAAcgcg                       <  1:447116/140‑1 (MQ=255)                                                                                                         cgcCGAGTGTCGCACCGCCCGATTCCCCCACAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTGTCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCgg                      <  2:1273232/140‑1 (MQ=255)                                                                                                         cgcCGAGTGTCGCACCGCCCGATTCCCCCACAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCgg                      <  2:1281342/140‑1 (MQ=255)                                                                                                           ccGAGTGTCGCACCGCCCGATTCCCCCACAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGa                    >  2:850873/1‑140 (MQ=255)                                                                                                                     cACCGCCCGATTCCCCCACAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAAct          <  2:638293/140‑1 (MQ=255)                                                                                                                          cccGATTCCCCCACAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTTCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACttcttc     <  1:1055925/140‑1 (MQ=255)                                                                                                                           ccGATTCCCCCACAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACTTCTTCa    <  2:247543/140‑1 (MQ=255)                                                                                                                             gATTCCCCCACAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACTTCTTCaaa  <  1:806464/140‑1 (MQ=255)                                                                                                                             gATTCCCCCACAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACTTCTTCaaa  <  1:850873/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                  CAGCACCTGATTGTTTTCCAGCTCCTGAGCAATTTCGGTTGTCATATCCGAACCGAAAACAGCAATTTTTCCGGCCTGATTTTGATTACGTACCGCTTTTACCGCGCCGAGTGTCGCACCGCCCGATTCCCCCATAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACTTCTTCAAA  >  NC_000913/2678561‑2678824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑