Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,705,998	G→A	T376I (ACC→ATC)	lepA ←	elongation factor 4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,705,998	0	G	A	98.9%	298.6 / ‑4.6	89	T376I (ACC→ATC)	lepA	elongation factor 4
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (41/47);  total (41/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.51e-01

ACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCG  >  NC_000913/2705864‑2706129                                                                                                                                       |                                                                                                                                    aCGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCa                                                                                                                                <  2:1310398/140‑1 (MQ=255) aCGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCa                                                                                                                                <  1:299360/140‑1 (MQ=255)  cGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCaa                                                                                                                               >  2:235151/1‑140 (MQ=255)  cGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCa                                                                                                                                >  1:355450/1‑139 (MQ=255)  cGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCa                                                                                                                                <  2:355450/139‑1 (MQ=255)   gTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAAc                                                                                                                              <  2:67711/140‑1 (MQ=255)    taataaCGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACt                                                                                                                             <  1:235151/140‑1 (MQ=255)      ataaCGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTc                                                                                                                           <  2:208850/140‑1 (MQ=255)       taaCGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCa                                                                                                                          <  2:1184010/140‑1 (MQ=255)          cGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATac                                                                                                                       >  2:64869/1‑140 (MQ=255)          cGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATac                                                                                                                       >  2:438375/1‑140 (MQ=255)          cGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATac                                                                                                                       >  1:713536/1‑140 (MQ=255)            ttGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGAttt                                                                                                                                  >  1:1372100/1‑127 (MQ=255)            ttGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGAttt                                                                                                                                  <  2:1046370/127‑1 (MQ=255)            ttGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGAttt                                                                                                                                  >  1:1046370/1‑127 (MQ=255)            ttGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGAttt                                                                                                                                  <  2:1372100/127‑1 (MQ=255)            ttGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATacac                                                                                                                     >  2:893120/1‑140 (MQ=255)            ttGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATacac                                                                                                                     >  1:639054/1‑140 (MQ=255)            ttGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATacac                                                                                                                     >  1:582139/1‑140 (MQ=255)            ttGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATacac                                                                                                                     >  1:203877/1‑140 (MQ=255)            ttGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATacac                                                                                                                     >  2:50512/1‑140 (MQ=255)            ttGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATacac                                                                                                                     >  1:1067900/1‑140 (MQ=255)              gCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGCTGTTATTTACCGCAGGTAGCTTGGATGGGCTGTCGGCGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTa                                                                                                                   >  1:319542/1‑140 (MQ=255)              gCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTa                                                                                                                   >  2:1405299/1‑140 (MQ=255)                    atatGCCTGCGGCAGCCGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCg                                                                                                             <  2:1323883/140‑1 (MQ=255)                       tGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGgcg                                                                                                          <  2:559619/140‑1 (MQ=255)                       tGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGgcg                                                                                                          <  1:438375/140‑1 (MQ=255)                       tGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGgcg                                                                                                          <  1:50512/140‑1 (MQ=255)                       tGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGgcg                                                                                                          >  1:252041/1‑140 (MQ=255)                       tGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGgcg                                                                                                          <  2:697119/140‑1 (MQ=255)                       tGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGgcg                                                                                                          <  1:1405299/140‑1 (MQ=255)                         ccTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCa                                                                                                        <  2:1355324/140‑1 (MQ=255)                            gCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCAgtg                                                                                                     <  1:558947/140‑1 (MQ=255)                             cGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCa                                                                                                        <  2:1474780/136‑1 (MQ=255)                             cGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCa                                                                                                        >  1:1474780/1‑136 (MQ=255)                             cGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCAgtgg                                                                                                    >  1:804717/1‑140 (MQ=255)                             cGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCAgtgg                                                                                                    <  2:639054/140‑1 (MQ=255)                             cGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCAgtgg                                                                                                    >  1:1464804/1‑140 (MQ=255)                              ggCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCAgtggt                                                                                                   >  2:1050417/1‑140 (MQ=255)                               gcagcagcaTGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCgg                                                                                                            >  1:81852/1‑130 (MQ=255)                               gcagcagcaTGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCgg                                                                                                            <  2:81852/130‑1 (MQ=255)                               gcagcagcaTGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCAgtggtg                                                                                                  <  2:1464804/140‑1 (MQ=255)                                 agcagcaTGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCAGTGGTGAt                                                                                                <  2:632343/140‑1 (MQ=255)                                 agcagcaTGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCAGTGGTGAt                                                                                                >  2:375549/1‑140 (MQ=255)                                 agcagcaTGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCAGTGGTGAt                                                                                                <  2:727955/140‑1 (MQ=255)                                 agcagcaTGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCAGTGGTGAt                                                                                                <  1:1438569/140‑1 (MQ=255)                                 agcagcaTGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCAGTGGTGAt                                                                                                >  2:1083477/1‑140 (MQ=255)                                 agcagcaTGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCAGTGGTGAt                                                                                                <  1:64869/140‑1 (MQ=255)                                   cagcaTGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATca                                                                                              >  1:338155/1‑140 (MQ=255)                                                tctGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTa                                                                                 >  2:1396568/1‑140 (MQ=255)                                                tctGCAATCGGCTCGCGCAGTTCCGAGATGATATTTACCGCAGGCCGGTTGGATGGGCTGTTGGCGCAGATAACTTTTCCTGTCGTGATTTCAACTTCATACACTACCGTCGGCGCCGTGGTGATcagatccaaaatgtt                                                                                 >  2:92186/1‑133 (MQ=255)                                                   gCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTc                                                                              <  2:338155/140‑1 (MQ=255)                                                    cAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCa                                                                             >  2:1472563/1‑140 (MQ=255)                                                    cAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCa                                                                             >  2:1447910/1‑140 (MQ=255)                                                    cAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCa                                                                             >  2:625934/1‑140 (MQ=255)                                                       tCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGt                                                                          >  1:559382/1‑140 (MQ=255)                                                        cGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGtt                                                                         <  1:92186/140‑1 (MQ=255)                                                          gCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTcc                                                                       >  1:1285735/1‑140 (MQ=255)                                                            tCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTc                                                                              <  1:1230123/131‑1 (MQ=255)                                                            tCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTc                                                                              >  2:1230123/1‑131 (MQ=255)                                                            tCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAg                                                                     >  1:806062/1‑140 (MQ=255)                                                            tCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAg                                                                     <  1:625934/140‑1 (MQ=255)                                                             cgcgCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGa                                                                    <  2:806062/140‑1 (MQ=255)                                                             cgcgCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGa                                                                    <  1:893120/140‑1 (MQ=255)                                                             cgcgCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGa                                                                    <  2:899227/140‑1 (MQ=255)                                                             cgcgCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGa                                                                    <  2:1395275/140‑1 (MQ=255)                                                              gcgcAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGAc                                                                   <  2:713536/140‑1 (MQ=255)                                                                    ttCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCt                                                             >  1:1216640/1‑140 (MQ=255)                                                                     tCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTg                                                            <  1:1396568/140‑1 (MQ=255)                                                                       gTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGga                                                          <  2:203877/140‑1 (MQ=255)                                                                        tAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGgat                                                         >  2:397352/1‑140 (MQ=255)                                                                        tAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGgat                                                         <  1:1083477/140‑1 (MQ=255)                                                                          gATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGgatga                                                       <  2:1216640/140‑1 (MQ=255)                                                                              ttATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGa                                                                    <  1:465226/123‑1 (MQ=255)                                                                              ttATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGa                                                                    >  2:465226/1‑123 (MQ=255)                                                                              ttATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGAtctc                                                   >  1:941957/1‑140 (MQ=255)                                                                               tATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTcc                                                  <  1:1050417/140‑1 (MQ=255)                                                                                      cGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTgca                                           <  2:1067900/140‑1 (MQ=255)                                                                                       gcaggcagCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTgcag                                          <  2:804717/140‑1 (MQ=255)                                                                                        caggcagCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTgcagc                                         >  1:1155028/1‑140 (MQ=255)                                                                                        caggcagCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTgcag                                          >  2:1461652/1‑139 (MQ=255)                                                                                        caggcagCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTgcag                                          <  1:1461652/139‑1 (MQ=255)                                                                                        caggcagCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGACCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTgcagc                                         <  2:252041/140‑1 (MQ=255)                                                                                           gcagCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAgg                                      <  1:397352/140‑1 (MQ=255)                                                                                                            tCGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCg                     >  1:1246178/1‑140 (MQ=255)                                                                                                             cGACGTAGATAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCgg                    >  1:1404188/1‑140 (MQ=255)                                                                                                                      tAACTTCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAg           >  2:679953/1‑140 (MQ=255)                                                                                                                          ttCTCTTGACGTGATTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCa       <  2:559382/140‑1 (MQ=255)                                                                                                                               ttGACGTGATTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCGGCGGAAACCAAAGCCCAgcgcg  <  2:436219/140‑1 (MQ=255)                                                                                                                               ttGACGTGATTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAgcgcg  <  2:1155028/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                    ACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCG  >  NC_000913/2705864‑2706129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑