Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,802,472	T→C	A375A (GCT→GCC)	nrdE →	ribonucleoside‑diphosphate reductase 2 subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,802,472	0	T	C	100.0%	320.5 / NA	92	A375A (GCT→GCC)	nrdE	ribonucleoside‑diphosphate reductase 2 subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (52/40);  total (52/40)

CACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGA  >  NC_000913/2802335‑2802608                                                                                                                                          |                                                                                                                                         cacTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCCaa                                                                                                                                        >  2:1345794/1‑140 (MQ=255)   cTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCCAAcc                                                                                                                                      <  2:624998/140‑1 (MQ=255)     aTGACGAACTGGCTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCCAACCCt                                                                                                                                    >  2:154029/1‑140 (MQ=255)           aaCTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCCAACCCTATCGcc                                                                                                                              <  2:239465/140‑1 (MQ=255)           aaCTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCCAACCCTATCGcc                                                                                                                              >  2:60531/1‑140 (MQ=255)           aaCTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCCAACCCTATCGcc                                                                                                                              >  1:164903/1‑140 (MQ=255)            aCTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCg                                                                                                                             >  1:64795/1‑140 (MQ=255)             cTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCgg                                                                                                                            <  2:24917/140‑1 (MQ=255)             cTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCgg                                                                                                                            <  1:60531/140‑1 (MQ=255)                gTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGgcg                                                                                                                         >  2:1249462/1‑140 (MQ=255)                gTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGgcg                                                                                                                         >  1:1456919/1‑140 (MQ=255)                  tGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCa                                                                                                                       <  1:1165133/140‑1 (MQ=255)                  tGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCa                                                                                                                       <  2:1238445/140‑1 (MQ=255)                  tGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCa                                                                                                                       <  2:1365271/140‑1 (MQ=255)                      gATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATaaa                                                                                                                   <  2:911101/140‑1 (MQ=255)                      gATGAACGCACTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATaaa                                                                                                                   <  2:662077/140‑1 (MQ=255)                        tGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGgcgc                                                                                                                        <  2:598362/133‑1 (MQ=255)                        tGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGgcgc                                                                                                                        >  1:598362/1‑133 (MQ=255)                        tGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAata                                                                                                                 <  2:164903/140‑1 (MQ=255)                               aTTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAata                                                                                                                 <  2:1449321/133‑1 (MQ=255)                               aTTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAata                                                                                                                 >  1:1449321/1‑133 (MQ=255)                                                 aaCGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAAtttt                                                                                        >  1:1150963/1‑140 (MQ=255)                                                 aaCGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAAtttt                                                                                        >  2:9649/1‑140 (MQ=255)                                                      ccGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAgg                                                                                   >  1:96612/1‑140 (MQ=255)                                                            tttCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACa                                                                             <  1:1245806/140‑1 (MQ=255)                                                             ttcttcCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAg                                                                            <  1:154029/140‑1 (MQ=255)                                                             ttcttcCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAg                                                                            <  1:933487/140‑1 (MQ=255)                                                               cttcCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAgcg                                                                          <  1:9649/140‑1 (MQ=255)                                                                  ccAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTTAGAAATTTTGCAGGTTAACAGCGCCt                                                                       <  1:1168295/140‑1 (MQ=255)                                                                   cAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTc                                                                      <  1:1249462/140‑1 (MQ=255)                                                                     gCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCag                                                                    >  1:284246/1‑140 (MQ=255)                                                                        aCTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGt                                                                 >  2:263734/1‑140 (MQ=255)                                                                          tGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTAt                                                               <  2:96612/140‑1 (MQ=255)                                                                          tGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTAt                                                               >  1:673975/1‑140 (MQ=255)                                                                                       gTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCg                                                  >  1:892956/1‑140 (MQ=255)                                                                                        tttGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAAtct                                                    >  2:1464045/1‑137 (MQ=255)                                                                                        tttGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAAtct                                                    <  1:1464045/137‑1 (MQ=255)                                                                                          tGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACt                                               >  1:158154/1‑140 (MQ=255)                                                                                            aGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACtat                                             >  1:1396823/1‑140 (MQ=255)                                                                                              tCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATAc                                           <  2:54026/140‑1 (MQ=255)                                                                                                  gCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGc                                       >  2:309209/1‑140 (MQ=255)                                                                                                   cTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGca                                      <  2:895459/140‑1 (MQ=255)                                                                                                   cTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGca                                      <  1:88451/140‑1 (MQ=255)                                                                                                   cTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGca                                      <  2:284246/140‑1 (MQ=255)                                                                                                    tATCCCTACATCATGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGcac                                     <  2:1396823/140‑1 (MQ=255)                                                                                                    tATCCCTACATCATGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGcac                                     <  2:1350625/140‑1 (MQ=255)                                                                                                    tATCCCTACATCATGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGcac                                     <  2:673975/140‑1 (MQ=255)                                                                                                     aTCCCTACATCATGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGcaca                                    <  2:64795/140‑1 (MQ=255)                                                                                                           aCATCATGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCAt                              >  2:1190366/1‑140 (MQ=255)                                                                                                            catcatGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATg                             >  1:9235/1‑140 (MQ=255)                                                                                                             atcatGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGca                                      <  1:226845/130‑1 (MQ=255)                                                                                                             atcatGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGca                                      >  2:226845/1‑130 (MQ=255)                                                                                                                atGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGatat                         >  2:478229/1‑140 (MQ=255)                                                                                                                atGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGatat                         >  1:452648/1‑140 (MQ=255)                                                                                                                atGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGatat                         >  1:422802/1‑140 (MQ=255)                                                                                                                atGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGatat                         >  1:742109/1‑140 (MQ=255)                                                                                                                atGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGatat                         >  1:1335067/1‑140 (MQ=255)                                                                                                                 tGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGcaca                                    <  2:722891/128‑1 (MQ=255)                                                                                                                 tGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGcaca                                    >  1:722891/1‑128 (MQ=255)                                                                                                                 tGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGc                                 <  2:279147/131‑1 (MQ=255)                                                                                                                 tGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGc                                 >  1:279147/1‑131 (MQ=255)                                                                                                                 tGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATAtt                        >  1:791183/1‑140 (MQ=255)                                                                                                                 tGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATAtt                        >  1:1241527/1‑140 (MQ=255)                                                                                                                 tGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATAtt                        >  1:584065/1‑140 (MQ=255)                                                                                                                 tGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATCACGAGAATCTCGACTATACCCGCACAGGCCATGATAtt                        >  1:979108/1‑140 (MQ=255)                                                                                                                 tGTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCACAGAAATTTTGTAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATAtt                        >  2:1298329/1‑140 (MQ=255)                                                                                                                  gTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATAttt                       >  1:197607/1‑140 (MQ=255)                                                                                                                  gTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATAttt                       >  2:1164130/1‑140 (MQ=255)                                                                                                                  gTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATAttt                       >  2:506068/1‑140 (MQ=255)                                                                                                                  gTATGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATAttt                       >  2:496214/1‑140 (MQ=255)                                                                                                                    aTGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTcc                     <  1:506068/140‑1 (MQ=255)                                                                                                                     tGAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCt                    >  2:736257/1‑140 (MQ=255)                                                                                                                      gAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTg                   <  2:422802/140‑1 (MQ=255)                                                                                                                      gAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTg                   >  2:6537/1‑140 (MQ=255)                                                                                                                      gAAGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTg                   >  2:1383415/1‑140 (MQ=255)                                                                                                                       aaGACACGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGc                  >  1:1387341/1‑140 (MQ=255)                                                                                                                           cacGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAAtt              >  2:817576/1‑140 (MQ=255)                                                                                                                           cacGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAAtt              >  1:21697/1‑140 (MQ=255)                                                                                                                            acGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAAttt             >  1:557566/1‑140 (MQ=255)                                                                                                                            acGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAAttt             >  1:307759/1‑140 (MQ=255)                                                                                                                             cGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTa            >  1:700956/1‑140 (MQ=255)                                                                                                                             cGGTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTa            >  2:1352230/1‑140 (MQ=255)                                                                                                                              ggTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAg           >  2:782101/1‑140 (MQ=255)                                                                                                                              ggTAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAg           <  2:1335067/140‑1 (MQ=255)                                                                                                                                tAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGt         <  2:742109/140‑1 (MQ=255)                                                                                                                                tAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGt         <  1:6537/140‑1 (MQ=255)                                                                                                                                tAAACCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGt         <  1:817576/140‑1 (MQ=255)                                                                                                                                  aaCCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTc       <  1:1190366/140‑1 (MQ=255)                                                                                                                                  aaCCGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATA‑CCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCg      >  2:983974/1‑140 (MQ=255)                                                                                                                                    ccGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGt     >  1:719524/1‑140 (MQ=255)                                                                                                                                     cGTGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGtt    <  2:557566/140‑1 (MQ=255)                                                                                                                                       tGCCAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGa  <  2:307759/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         CACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGA  >  NC_000913/2802335‑2802608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑