Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	134,603	G→A	V72V (GTG→GTA)	yacL →	UPF0231 family protein YacL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	134,603	0	G	A	100.0%	312.0 / NA	89	V72V (GTG→GTA)	yacL	UPF0231 family protein YacL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (43/46);  total (43/46)

GGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTGATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTGGCGGCTTACCGCAATTTCGTG  >  NC_000913/134467‑134738                                                                                                                                         |                                                                                                                                        ggTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATg                                                                                                                                      >  1:879203/1‑140 (MQ=255)  gTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATgg                                                                                                                                     >  2:1171658/1‑140 (MQ=255)   tttAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGt                                                                                                                                    <  1:1198193/140‑1 (MQ=255)           agagGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCa                                                                                                                            <  2:1280149/140‑1 (MQ=255)            gagGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCaa                                                                                                                           >  2:274784/1‑140 (MQ=255)            gagGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCaa                                                                                                                           >  1:953439/1‑140 (MQ=255)            gagGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCa                                                                                                                            <  1:773863/139‑1 (MQ=255)            gagGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCa                                                                                                                            >  2:773863/1‑139 (MQ=255)             agGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAAt                                                                                                                          >  2:1420276/1‑140 (MQ=255)                tGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATc                                                                                                                         >  1:767217/1‑138 (MQ=255)                tGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATc                                                                                                                         <  2:767217/138‑1 (MQ=255)                 gaaagaaaACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAAc                                                                                                                      <  1:1473039/140‑1 (MQ=255)                 gaaagaaaACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAAc                                                                                                                      <  1:1245188/140‑1 (MQ=255)                  aaagaaaACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTcg                                                                                                                                 <  1:548375/128‑1 (MQ=255)                  aaagaaaACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTcg                                                                                                                                 >  2:548375/1‑128 (MQ=255)                  aaagaaaACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACt                                                                                                                     >  2:373524/1‑140 (MQ=255)                       aaaCCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCaa                                                                                                                       >  1:544682/1‑133 (MQ=255)                       aaaCCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCaa                                                                                                                       <  2:544682/133‑1 (MQ=255)                       aaaCCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAAt                                                                                                                >  2:106799/1‑140 (MQ=255)                         aCCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTc                                                                                                              >  1:158943/1‑140 (MQ=255)                            tGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCt                                                                                                           >  2:1445821/1‑140 (MQ=255)                             ggCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTg                                                                                                          <  2:616807/140‑1 (MQ=255)                                 ttgcttgATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGa                                                                                                      <  2:15842/140‑1 (MQ=255)                                 ttgcttgATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGa                                                                                                      <  1:106799/140‑1 (MQ=255)                                    cttgATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACGCCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGa                                                                                                   <  1:1171658/140‑1 (MQ=255)                                       gatgaAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAAt                                                                                                <  1:1036576/140‑1 (MQ=255)                                        atgaAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATg                                                                                               >  2:11121/1‑140 (MQ=255)                                         tgaAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATgg                                                                                              <  2:1197280/140‑1 (MQ=255)                                            aGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAag                                                                                           <  2:879203/140‑1 (MQ=255)                                             gTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGaa                                                                                                  >  2:694305/1‑132 (MQ=255)                                             gTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGaa                                                                                                  <  1:694305/132‑1 (MQ=255)                                               ggAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTTGCTGGCGATGAAATGGAAGAgg                                                                                        <  2:1293658/140‑1 (MQ=255)                                                 aaCAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAgggg                                                                                      <  2:953439/140‑1 (MQ=255)                                                 aaCAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAgggg                                                                                      <  1:373524/140‑1 (MQ=255)                                                  aCAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGa                                                                                     <  2:1139689/140‑1 (MQ=255)                                                  aCAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGa                                                                                     <  1:351589/140‑1 (MQ=255)                                                  aCAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGa                                                                                     <  1:1282076/140‑1 (MQ=255)                                                  aCAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGa                                                                                     <  1:11121/140‑1 (MQ=255)                                                   cAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGAt                                                                                    >  1:1123148/1‑140 (MQ=255)                                                    aaGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATg                                                                                   <  2:1123148/140‑1 (MQ=255)                                                    aaGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATg                                                                                   <  1:122530/140‑1 (MQ=255)                                                          cgcacgcaCTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAactac                                                                             >  2:1374384/1‑140 (MQ=255)                                                          cgcacgcaCTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAactac                                                                             >  1:1282164/1‑140 (MQ=255)                                                          cgcacgcaCTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAac                                                                                <  2:1158134/137‑1 (MQ=255)                                                          cgcacgcaCTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAac                                                                                >  1:1158134/1‑137 (MQ=255)                                                              cgcacTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTacg                                                                         <  2:1050282/140‑1 (MQ=255)                                                               gcacTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTacga                                                                        <  2:290835/140‑1 (MQ=255)                                                               gcacTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTacga                                                                        <  1:1333098/140‑1 (MQ=255)                                                                    gAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACgaag                                                                   <  2:21699/140‑1 (MQ=255)                                                                       aGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGaaa                                                                <  2:158943/140‑1 (MQ=255)                                                                       aGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGaaa                                                                <  1:1374384/140‑1 (MQ=255)                                                                         gTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGc                                                              <  1:1091797/140‑1 (MQ=255)                                                                           aGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAGTGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTacg                                                                         >  2:1043658/1‑127 (MQ=255)                                                                           aGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAGTGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTacg                                                                         <  1:1043658/127‑1 (MQ=255)                                                                           aGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCt                                                            >  2:280105/1‑140 (MQ=255)                                                                             cGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGt                                                          >  1:1017447/1‑140 (MQ=255)                                                                               aaCGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCg                                                        >  1:1329227/1‑140 (MQ=255)                                                                               aaCGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCg                                                        <  1:1419675/140‑1 (MQ=255)                                                                               aaCGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCg                                                        >  2:1419675/1‑140 (MQ=255)                                                                               aaCGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCg                                                        >  1:87479/1‑140 (MQ=255)                                                                                   gTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGAAATGGGTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTAt                                                    >  2:71653/1‑140 (MQ=255)                                                                                    tCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATg                                                   >  2:944671/1‑140 (MQ=255)                                                                                      cTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATgcg                                                 <  1:944671/140‑1 (MQ=255)                                                                                      cTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATgcg                                                 >  1:749685/1‑140 (MQ=255)                                                                                             cGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGa                                          <  1:1420276/140‑1 (MQ=255)                                                                                              gggcagggcaTGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATgcg                                                 >  1:870737/1‑132 (MQ=255)                                                                                              gggcagggcaTGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATgcg                                                 <  2:870737/132‑1 (MQ=255)                                                                                              gggcagggcaTGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAg                                         >  1:140739/1‑140 (MQ=255)                                                                                              gggcagggcaTGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAg                                         >  1:507101/1‑140 (MQ=255)                                                                                              gggcagggcaTGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAg                                         >  1:1458505/1‑140 (MQ=255)                                                                                                   gggcaTGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGAttt                                    >  1:821189/1‑140 (MQ=255)                                                                                                       aTGAATACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTg                                >  2:254282/1‑140 (MQ=255)                                                                                                            tACACCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGt                           <  2:821189/140‑1 (MQ=255)                                                                                                              cacCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCg                         >  2:1013791/1‑140 (MQ=255)                                                                                                               acCCTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGt                        >  1:783428/1‑140 (MQ=255)                                                                                                                cccTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTg                                >  2:1123986/1‑131 (MQ=255)                                                                                                                cccTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTg                                <  1:1123986/131‑1 (MQ=255)                                                                                                                cccTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTg                       <  2:329495/140‑1 (MQ=255)                                                                                                                cccTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTg                       >  1:329495/1‑140 (MQ=255)                                                                                                                cccTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTg                       <  1:1013791/140‑1 (MQ=255)                                                                                                                  cTGTGGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTggc                     >  2:38123/1‑140 (MQ=255)                                                                                                                   tgtgGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTggcg                    <  1:280105/140‑1 (MQ=255)                                                                                                                    gtgGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTggcgg                   <  1:274784/140‑1 (MQ=255)                                                                                                                    gtgGATGGACGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTggcgg                   >  2:1181387/1‑140 (MQ=255)                                                                                                                      ggatggaCGGTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTGGCGGCt                 <  1:827332/140‑1 (MQ=255)                                                                                                                               gTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTGGCGGCTTACCGCAAt        >  2:305465/1‑140 (MQ=255)                                                                                                                               gTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTGGCGGCTTACCGCAAt        >  1:324772/1‑140 (MQ=255)                                                                                                                               gTGAAGAGGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTGGCGGCTTACCGCAAt        >  2:1072555/1‑140 (MQ=255)                                                                                                                                   agagGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTGGCGGCTTACCGCAATTTCg    <  1:254282/140‑1 (MQ=255)                                                                                                                                     agGTAATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTGGCGGCTTACCGCAATTTCGTg  <  2:324772/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        GGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTGATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTGGCGGCTTACCGCAATTTCGTG  >  NC_000913/134467‑134738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑