Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,824,038	A→G	S103S (AGT→AGC)	pncC ←	NMN aminohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,824,038	0	A	G	100.0%	259.9 / NA	76	S103S (AGT→AGC)	pncC	NMN aminohydrolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/40);  total (36/40)

CTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCACTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGTCTCTTCGCGTACGCCGATCATCTGCGCTTTGGCTTCGT  >  NC_000913/2823903‑2824174                                                                                                                                        |                                                                                                                                         cTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCGCTaa                                                                                                                                      <  1:1153379/140‑1 (MQ=255)  tGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAAt                                                                                                                                     <  1:1006715/140‑1 (MQ=255)   gacgacGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATa                                                                                                                                    >  1:368566/1‑140 (MQ=255)    acgacgCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAg                                                                                                                                   <  1:1078432/140‑1 (MQ=255)     cgacgCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGa                                                                                                                                  >  2:655525/1‑140 (MQ=255)      gacgCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGac                                                                                                                                 <  1:892747/140‑1 (MQ=255)           aCCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGc                                                                                                                            <  1:735513/140‑1 (MQ=255)               cATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATaa                                                                                                                        >  2:291112/1‑140 (MQ=255)                  cACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCa                                                                                                                     >  1:1298580/1‑140 (MQ=255)                  cACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCa                                                                                                                     >  2:740830/1‑140 (MQ=255)                      gTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCAc                                                                                                                 <  1:778658/140‑1 (MQ=255)                      gTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCAc                                                                                                                 <  1:999043/140‑1 (MQ=255)                       tCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACg                                                                                                                >  1:136665/1‑140 (MQ=255)                        cgccgcTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGa                                                                                                               <  1:28984/140‑1 (MQ=255)                         gccgcTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGAg                                                                                                              <  1:220502/140‑1 (MQ=255)                         gccgcTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGAg                                                                                                              >  1:259963/1‑140 (MQ=255)                         gccgcTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGAg                                                                                                              >  1:1084948/1‑140 (MQ=255)                          ccgcTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGAGc                                                                                                             <  2:457608/140‑1 (MQ=255)                               gAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCtt                                                                                                        >  2:49763/1‑140 (MQ=255)                                 aGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTc                                                                                                      <  2:775993/140‑1 (MQ=255)                                 aGCATGCCCGCGGGGGAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCCGCGCCGCCATCCGGCCCGGGGATCCCGCTAATAGACAGGGCATAATCAGCACGAGCCGCTTTc                                                                                                      <  2:1318933/140‑1 (MQ=255)                                     ttCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGGGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTg                                                                                                  <  2:113487/140‑1 (MQ=255)                                     ttCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTg                                                                                                  <  1:49763/140‑1 (MQ=255)                                     ttCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTg                                                                                                  <  2:859148/140‑1 (MQ=255)                                     ttCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTg                                                                                                  <  2:463744/140‑1 (MQ=255)                                     ttCCCGCCGGGAAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTg                                                                                                  >  1:1363170/1‑140 (MQ=255)                                          gccgGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCt                                                                                             >  1:609921/1‑140 (MQ=255)                                             gggTAATGCCTTCACCGCGGGCCGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATc                                                                                          <  2:1084948/140‑1 (MQ=255)                                             gggTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATc                                                                                          <  1:1022964/140‑1 (MQ=255)                                              ggTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCg                                                                                         <  2:223977/140‑1 (MQ=255)                                               gTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCGc                                                                                        >  2:591478/1‑140 (MQ=255)                                                tAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTACTCTTCACGGCCGCCATGCGGCCCGGCGATACGGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCGcc                                                                                       <  2:481056/140‑1 (MQ=255)                                                    gCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCGCCAttt                                                                                   >  1:1361527/1‑140 (MQ=255)                                                       ttCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCGCCATTTcca                                                                                <  1:563027/140‑1 (MQ=255)                                                        tCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCCCGAGCCGCTTTCAGTGCGCCTATCGCCATTTccac                                                                               <  2:250883/140‑1 (MQ=255)                                                        tCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCGCCATTTccac                                                                               >  1:1022908/1‑140 (MQ=255)                                                        tCACCGCGGGCAGTGGCAAAAGCAAACCAGAAGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCGCCATTTccac                                                                               >  2:111177/1‑140 (MQ=255)                                                         cACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCGCCATTTccacc                                                                              <  2:94425/140‑1 (MQ=255)                                                           ccGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCGCCATTTCcaccac                                                                            <  1:179460/140‑1 (MQ=255)                                                             gcgGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCGCCATTTCCACCacga                                                                          <  1:477993/140‑1 (MQ=255)                                                             gcgGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCGCCATTTCCACCacga                                                                          >  2:440384/1‑140 (MQ=255)                                                              cgGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCGCCATTTCCACCacgac                                                                         >  1:1350881/1‑140 (MQ=255)                                                              cgGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCGCCATTTCCACCacgac                                                                         >  1:694764/1‑140 (MQ=255)                                                               gggCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGGGATCCCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCGCCATTTCCACCacgacg                                                                        <  2:1330604/140‑1 (MQ=255)                                                                ggCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACgg                                                                       >  2:28308/1‑140 (MQ=255)                                                                    gTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTtc                                                                   >  1:377833/1‑140 (MQ=255)                                                                         aaaaGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATTATCAGCACGAGCCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTtcactca                                                              <  2:1022908/140‑1 (MQ=255)                                                                         aaaaGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTtcactca                                                              <  1:740830/140‑1 (MQ=255)                                                                             gCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACcgc                                                          >  1:1189665/1‑140 (MQ=255)                                                                              cAAACCAGCCGTTGCCGACAGGCTCCTCTCCACCGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACcgcg                                                         <  2:1363170/140‑1 (MQ=255)                                                                               aaaCCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCgcgc                                                        <  1:655525/140‑1 (MQ=255)                                                                                       cGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGTGTTCACTCACCGCGCCAtgctgc                                                <  1:440384/140‑1 (MQ=255)                                                                                             cGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGcc                                                       >  1:62781/1‑127 (MQ=255)                                                                                             cGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGcc                                                       <  2:62781/127‑1 (MQ=255)                                                                                             cGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGTTGCGCCAGc                                          <  2:694764/140‑1 (MQ=255)                                                                                             cGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGc                                          >  2:1437098/1‑140 (MQ=255)                                                                                             cGACAGGCTTCCCTTCACTCCCGCCATCCGCCCGGGCGATACCGCAAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGc                                          <  2:1361527/140‑1 (MQ=255)                                                                                                   gCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGTctct                                    <  2:368566/140‑1 (MQ=255)                                                                                                    cTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGTCTCtt                                   >  1:447725/1‑140 (MQ=255)                                                                                                      tctcTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGTCTCTTcg                                 >  1:867148/1‑140 (MQ=255)                                                                                                      tctcTTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGTCTCTTcg                                 >  2:1193953/1‑140 (MQ=255)                                                                                                        tctTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGTCTCTTcgcg                               <  2:867148/140‑1 (MQ=255)                                                                                                          ttCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGTCTCTTCGCGTa                             <  1:28308/140‑1 (MQ=255)                                                                                                            cACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGTCTCTTCGCGTACg                           <  2:447725/140‑1 (MQ=255)                                                                                                             aCTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGc                                          >  1:489179/1‑124 (MQ=255)                                                                                                             aCTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGc                                          <  2:489179/124‑1 (MQ=255)                                                                                                             aCTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGTCTCTTCGCGTACGc                          >  1:1091012/1‑140 (MQ=255)                                                                                                               tGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGtctc                                     <  2:507783/127‑1 (MQ=255)                                                                                                               tGCCGCCATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGtctc                                     >  1:507783/1‑127 (MQ=255)                                                                                                                 ccgccATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGTCTCTTCGCGTACGCCGat                      >  1:371689/1‑140 (MQ=255)                                                                                                                 ccgccATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGTCTCTTCGCGTACGCCGat                      >  1:999748/1‑140 (MQ=255)                                                                                                                 ccgccATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGTCTCTTCGCGTACGCCGat                      >  1:704242/1‑140 (MQ=255)                                                                                                                    ccATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGTCTCTTCGCGTACGCCGatcat                   >  2:435678/1‑140 (MQ=255)                                                                                                                     cATCCGGCCCGGCGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGTCTCTTCGCGTACGCCGatcatc                  <  1:878317/140‑1 (MQ=255)                                                                                                                           gcccggcGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGTCTCTTCGCGTACGCCGATCATCTGCGCt            >  1:1415626/1‑140 (MQ=255)                                                                                                                            cccggcGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGTCTCTTCGCGTACGCCGATCATCTGCGCtt           >  1:917238/1‑140 (MQ=255)                                                                                                                                gcGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGTCTCTTCGCGTACGCCGATCATCTGCGCTTTGGc       <  2:1189665/140‑1 (MQ=255)                                                                                                                                gcGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGTCTCTTCGCGTACGCCGATCATCTGCGCTTTGGc       <  1:1193953/140‑1 (MQ=255)                                                                                                                                 cGATACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGTCTCTTCGCGTACGCCGATCATCTGCGCTTTGGCt      >  2:436939/1‑140 (MQ=255)                                                                                                                                    tACCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGTCTCTTCGCGTACGCCGATCATCTGCGCTTTGGCTTCg   <  2:917238/140‑1 (MQ=255)                                                                                                                                     aCCGCTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGTCTCTTCGCGTACGCCGATCATCTGCGCTTTGGCTTCGt  <  1:435678/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         CTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCACTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGTCTCTTCGCGTACGCCGATCATCTGCGCTTTGGCTTCGT  >  NC_000913/2823903‑2824174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑