Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,910,723	A→G	intergenic (‑38/+33)	mazG ← / ← mazF	nucleoside triphosphate pyrophosphohydrolase/endoribonuclease toxin MazF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,910,723	0	A	G	100.0%	275.1 / NA	79	intergenic (‑38/+33)	mazG/mazF	nucleoside triphosphate pyrophosphohydrolase/endoribonuclease toxin MazF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/43);  total (36/43)

CGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTT  >  NC_000913/2910587‑2910857                                                                                                                                         |                                                                                                                                       cgcAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCt                                                                                                                                     <  1:179960/140‑1 (MQ=255)  gcAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCtg                                                                                                                                    >  2:31761/1‑140 (MQ=255)   cAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCtgt                                                                                                                                   >  1:1187893/1‑140 (MQ=255)    aaTGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCt                                                                                                                                     <  2:838805/137‑1 (MQ=255)    aaTGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCt                                                                                                                                     >  1:838805/1‑137 (MQ=255)      tggtggCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGAc                                                                                                                                <  1:936046/140‑1 (MQ=255)      tggtggCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGAc                                                                                                                                <  2:1025949/140‑1 (MQ=255)      tggtggCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGAc                                                                                                                                <  1:31761/140‑1 (MQ=255)         tggCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAg                                                                                                                             >  2:409293/1‑140 (MQ=255)             aaaTGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATa                                                                                                                         <  2:1187893/140‑1 (MQ=255)               aTGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGa                                                                                                                       <  1:971220/140‑1 (MQ=255)                tGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGaa                                                                                                                      >  1:1351823/1‑140 (MQ=255)                  tCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGt                                                                                                                    >  1:204342/1‑140 (MQ=255)                    tGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGa                                                                                                                  <  2:1351823/140‑1 (MQ=255)                    tGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGa                                                                                                                  <  1:949705/140‑1 (MQ=255)                    tGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGa                                                                                                                  <  1:426121/140‑1 (MQ=255)                    tGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGa                                                                                                                  <  2:378559/140‑1 (MQ=255)                    tGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGa                                                                                                                  <  1:1309739/140‑1 (MQ=255)                    tGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATCGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGa                                                                                                                  <  2:1142247/140‑1 (MQ=255)                     gCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGa                                                                                                                  <  2:1265487/139‑1 (MQ=255)                     gCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGa                                                                                                                  >  1:1265487/1‑139 (MQ=255)                         tttATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGAGTTAg                                                                                                             >  2:1255528/1‑140 (MQ=255)                             tCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAac                                                                                                         <  1:820579/140‑1 (MQ=255)                              cccACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAaca                                                                                                        <  1:872324/140‑1 (MQ=255)                              cccACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAaca                                                                                                        <  1:164603/140‑1 (MQ=255)                               ccACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAacac                                                                                                       <  2:204342/140‑1 (MQ=255)                                cACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAACACt                                                                                                      >  1:48921/1‑140 (MQ=255)                                  cGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTAc                                                                                                    <  1:1285840/140‑1 (MQ=255)                                  cGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTAc                                                                                                    <  1:792356/140‑1 (MQ=255)                                   gggCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGt                                                                                                                    <  2:1412267/123‑1 (MQ=255)                                   gggCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGt                                                                                                                    >  1:1412267/1‑123 (MQ=255)                                   gggCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAacac                                                                                                       >  1:703020/1‑136 (MQ=255)                                   gggCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAacac                                                                                                       <  2:703020/136‑1 (MQ=255)                                     gCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAGCACTACCCa                                                                                                 >  2:168933/1‑140 (MQ=255)                                     gCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCa                                                                                                 >  1:1140654/1‑140 (MQ=255)                                     gCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCa                                                                                                 >  1:404178/1‑140 (MQ=255)                                     gCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCa                                                                                                 >  1:424841/1‑140 (MQ=255)                                         ccGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCa                                                                                             <  2:404178/140‑1 (MQ=255)                                              ttCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACg                                                                                        >  1:202025/1‑140 (MQ=255)                                                      cgcgCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGCAGTGAGTTAGTAACACTACCCAATCAGTACGTTAAtttt                                                                                >  2:123087/1‑140 (MQ=255)                                                       gcgcAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTg                                                                               <  2:202025/140‑1 (MQ=255)                                                          cAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCt                                                                            <  2:48921/140‑1 (MQ=255)                                                               gcTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAAt                                                                       <  1:123087/140‑1 (MQ=255)                                                                cTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATg                                                                      <  2:424841/140‑1 (MQ=255)                                                                cTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATg                                                                      >  1:859797/1‑140 (MQ=255)                                                                   cATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATg                                                                      >  2:37501/1‑137 (MQ=255)                                                                   cATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATg                                                                      <  1:37501/137‑1 (MQ=255)                                                                       ataGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATg                                                                      <  1:125068/133‑1 (MQ=255)                                                                       ataGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATg                                                                      >  2:125068/1‑133 (MQ=255)                                                                             aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCct                                                         >  1:796504/1‑140 (MQ=255)                                                                             aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCct                                                         >  1:1313643/1‑140 (MQ=255)                                                                             aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCct                                                         >  1:1276510/1‑140 (MQ=255)                                                                             aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCct                                                         >  2:256655/1‑140 (MQ=255)                                                                             aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCct                                                         >  2:110603/1‑140 (MQ=255)                                                                             aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCct                                                         >  2:784198/1‑140 (MQ=255)                                                                             aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCct                                                         >  1:69573/1‑140 (MQ=255)                                                                             aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCct                                                         >  2:662920/1‑140 (MQ=255)                                                                             aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGCaa                                                              <  2:1094359/135‑1 (MQ=255)                                                                             aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGCaa                                                              >  1:1094359/1‑135 (MQ=255)                                                                             aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAACAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCct                                                         >  2:255587/1‑140 (MQ=255)                                                                               cAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCctct                                                       >  2:392023/1‑140 (MQ=255)                                                                                   cGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTgggg                                                   <  1:327100/140‑1 (MQ=255)                                                                                    ggTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGc                                                  <  1:775020/140‑1 (MQ=255)                                                                                     gTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCa                                                 >  2:1325801/1‑140 (MQ=255)                                                                                      tCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCaa                                                <  1:725686/140‑1 (MQ=255)                                                                                       cGATTTGATTCATTGAATGGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAAc                                               <  2:1313643/140‑1 (MQ=255)                                                                                              aTTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCt                                        >  2:712039/1‑140 (MQ=255)                                                                                                  aTTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTttct                                    <  1:409293/140‑1 (MQ=255)                                                                                                   ttGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTttctt                                   <  2:1276510/140‑1 (MQ=255)                                                                                                      aaTTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCATTTCTTCGt                                >  2:48255/1‑140 (MQ=255)                                                                                                        ttGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTg                              <  1:712039/140‑1 (MQ=255)                                                                                                             cTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTg                              <  2:1229858/135‑1 (MQ=255)                                                                                                             cTGAAAATTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTg                              >  1:1229858/1‑135 (MQ=255)                                                                                                                aaaaTTGCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCtt                      >  2:1423081/1‑140 (MQ=255)                                                                                                                      gCGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGc                <  1:1325801/140‑1 (MQ=255)                                                                                                                       cGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGcc               <  2:993828/140‑1 (MQ=255)                                                                                                                       cGGGTCTGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCATTTCTTCGTTGCTCCTCTTGCCCGcc               <  1:48255/140‑1 (MQ=255)                                                                                                                             tGTCAGGTGGAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGa         <  1:1195344/140‑1 (MQ=255)                                                                                                                                    tGGAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACtttt  >  2:110306/1‑140 (MQ=255)                                                                                                                                      gAAGCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGa         <  2:702540/131‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       CGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTT  >  NC_000913/2910587‑2910857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑