BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,925,012	T→C	H90R (CAC→CGC)	syd ←	SecY‑interacting protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,925,012	0	T	C	99.0%	345.0 / NA	100	H90R (CAC→CGC)	syd	SecY‑interacting protein
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (40/59); total (41/59)
Fisher's exact test for biased strand distribution p-value = 4.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 109 total aligned reads displayed.

CGATAAATAGTGTGGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGT  >  NC_000913/2924878‑2925148

cgATAAATAGTGTGGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTgcgcgcgc                                                                                                                                        >  1:273679/1‑137 (MQ=255)

   tAAATAGTGTGGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTACACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATcc                                                                                                                                  <  1:976130/140‑1 (MQ=255)

      aTAGTGTGGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATCACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCg                                                                                                                               <  2:1378879/140‑1 (MQ=255)

          tgtgGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATcc                                                                                                                                  <  1:1292722/133‑1 (MQ=255)

          tgtgGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCaa                                                                                                                           >  2:966899/1‑140 (MQ=255)

          tgtgGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCaa                                                                                                                           >  1:1260729/1‑140 (MQ=255)

          tgtgGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCaa                                                                                                                           >  1:125874/1‑140 (MQ=255)

          tgtgGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTACAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATcc                                                                                                                                  >  2:1292722/1‑133 (MQ=255)

            tgGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAAc                                                                                                                         >  2:386212/1‑140 (MQ=255)

             gggCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACt                                                                                                                        <  2:920011/140‑1 (MQ=255)

             gggCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACt                                                                                                                        >  1:912630/1‑140 (MQ=255)

              ggcggcAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTg                                                                                                                       >  2:1246566/1‑140 (MQ=255)

              ggcggcAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTg                                                                                                                       >  1:570201/1‑140 (MQ=255)

              ggcggcAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTg                                                                                                                       >  1:172729/1‑140 (MQ=255)

              ggcggcAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTg                                                                                                                       >  2:288373/1‑140 (MQ=255)

               gcggcAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACt                                                                                                                        >  2:270838/1‑138 (MQ=255)

               gcggcAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACt                                                                                                                        <  1:270838/138‑1 (MQ=255)

               gcggcAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGa                                                                                                                      >  1:389406/1‑140 (MQ=255)

                 ggcAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGt                                                                                                                    <  2:912630/140‑1 (MQ=255)

                 ggcAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGt                                                                                                                    <  1:1197561/140‑1 (MQ=255)

                 ggcAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGt                                                                                                                    <  2:170240/140‑1 (MQ=255)

                  gcAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTc                                                                                                                   >  2:675127/1‑140 (MQ=255)

                     gTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGta                                                                                                                <  1:1312221/140‑1 (MQ=255)

                      ttttAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGtat                                                                                                               <  2:570201/140‑1 (MQ=255)

                       tttAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGtata                                                                                                              <  1:675127/140‑1 (MQ=255)

                        ttAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATaa                                                                                                             <  1:1461334/140‑1 (MQ=255)

                          aGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATaaaa                                                                                                           <  2:936170/140‑1 (MQ=255)

                             cTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGt                                                                                                        <  1:755808/140‑1 (MQ=255)

                              ttttCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTa                                                                                                       <  1:340765/140‑1 (MQ=255)

                               tttCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTAt                                                                                                      <  2:172729/140‑1 (MQ=255)

                               tttCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTAt                                                                                                      <  2:125874/140‑1 (MQ=255)

                               tttCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTAt                                                                                                      <  1:571438/140‑1 (MQ=255)

                               tttCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTAt                                                                                                      <  1:1416664/140‑1 (MQ=255)

                                ttCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAg                                                                                                                     >  1:169598/1‑124 (MQ=255)

                                ttCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAg                                                                                                                     <  2:169598/124‑1 (MQ=255)

                                ttCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGt                                                                                                                    >  2:94738/1‑125 (MQ=255)

                                ttCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGt                                                                                                                    <  1:94738/125‑1 (MQ=255)

                                ttCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATg                                                                                                     >  1:453645/1‑140 (MQ=255)

                                ttCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATg                                                                                                     <  2:389406/140‑1 (MQ=255)

                                ttCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACAGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATg                                                                                                     >  1:1154909/1‑140 (MQ=255)

                                 tCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGa                                                                                                    <  2:1025678/140‑1 (MQ=255)

                                    gAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAAtt                                                                                                 <  2:1359528/140‑1 (MQ=255)

                                    gAGTAACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAAtt                                                                                                 <  2:1008416/140‑1 (MQ=255)

                                      gTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGtata                                                                                                              <  2:319571/125‑1 (MQ=255)

                                      gTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAG‑TTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGtata                                                                                                              <  2:745818/124‑1 (MQ=255)

                                      gTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAG‑TTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGtata                                                                                                              >  1:745818/1‑124 (MQ=255)

                                       tCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATtgtg                                                                                              <  2:282184/140‑1 (MQ=255)

                                       tCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATtgtg                                                                                              <  2:1021559/140‑1 (MQ=255)

                                         aCCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTggg                                                                                            <  1:1051636/140‑1 (MQ=255)

                                         aCCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTggg                                                                                            <  1:383916/140‑1 (MQ=255)

                                         aCCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTggg                                                                                            <  2:261301/140‑1 (MQ=255)

                                         aCCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTggg                                                                                            <  1:446684/140‑1 (MQ=255)

                                         aCCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTggg                                                                                            <  1:324252/140‑1 (MQ=255)

                                         aCCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTggg                                                                                            <  1:150301/140‑1 (MQ=255)

                                          ccAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGt                                                                                           >  2:1010592/1‑140 (MQ=255)

                                          ccAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGt                                                                                           >  1:1477600/1‑140 (MQ=255)

                                               tGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTAt                                                                                      <  2:457709/140‑1 (MQ=255)

                                               tGACCAATCAGATTTTCCTGCACCCGACAGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTAt                                                                                      <  2:1154909/140‑1 (MQ=255)

                                                gACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATc                                                                                     <  2:670203/140‑1 (MQ=255)

                                                gACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATc                                                                                     <  2:788889/140‑1 (MQ=255)

                                                 aCCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATca                                                                                    >  2:1075959/1‑140 (MQ=255)

                                                  ccAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATcac                                                                                   >  2:901049/1‑140 (MQ=255)

                                                  ccAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATcac                                                                                   <  2:293908/140‑1 (MQ=255)

                                                     aTCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAat                                                                                >  2:643612/1‑140 (MQ=255)

                                                     aTCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTAt                                                                                      <  2:591657/134‑1 (MQ=255)

                                                     aTCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTAt                                                                                      >  1:591657/1‑134 (MQ=255)

                                                     aTCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAat                                                                                >  2:833052/1‑140 (MQ=255)

                                                     aTCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAat                                                                                >  1:752624/1‑140 (MQ=255)

                                                      tCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAata                                                                               >  2:1002199/1‑140 (MQ=255)

                                                                       cGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAAc                                                              <  2:804415/140‑1 (MQ=255)

                                                                        gACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAAcc                                                             >  2:372123/1‑140 (MQ=255)

                                                                         aCGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACcg                                                            <  1:833052/140‑1 (MQ=255)

                                                                                tcgtcTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTAc                                                     >  1:895994/1‑140 (MQ=255)

                                                                                        aCTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGtt                                             <  1:966899/140‑1 (MQ=255)

                                                                                                tttGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTa                                     <  2:657827/140‑1 (MQ=255)

                                                                                                  tGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGt                                              <  1:257801/129‑1 (MQ=255)

                                                                                                    cagcaATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACg                                 >  1:1110644/1‑140 (MQ=255)

                                                                                                    cagcaATGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACg                                 >  1:674484/1‑140 (MQ=255)

                                                                                                        aaTGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTg                             <  1:1002199/140‑1 (MQ=255)

                                                                                                         aTGTCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTgg                            >  1:446688/1‑140 (MQ=255)

                                                                                                            tCAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGt                         >  1:402172/1‑140 (MQ=255)

                                                                                                             cAGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGtt                        <  1:372123/140‑1 (MQ=255)

                                                                                                              aGTTTGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTg                       <  2:1234297/140‑1 (MQ=255)

                                                                                                                 ttGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTa                                     <  2:463542/123‑1 (MQ=255)

                                                                                                                 ttGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCa                    <  1:1246566/140‑1 (MQ=255)

                                                                                                                  tGATATCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAg                   <  1:1075959/140‑1 (MQ=255)

                                                                                                                    atatCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACgg                                <  1:889337/125‑1 (MQ=255)

                                                                                                                    atatCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACgg                                >  2:889337/1‑125 (MQ=255)

                                                                                                                     tatCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTg                             <  1:1370278/127‑1 (MQ=255)

                                                                                                                     tatCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTg                             >  2:1370278/1‑127 (MQ=255)

                                                                                                                     tatCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGtat                >  2:305674/1‑140 (MQ=255)

                                                                                                                       tCGCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATAc              <  1:305674/140‑1 (MQ=255)

                                                                                                                         gCCAAACTGCGCGCGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACgg            >  2:1442522/1‑140 (MQ=255)

                                                                                                                                 gcgcgcgcATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCg    <  2:895994/140‑1 (MQ=255)

                                                                                                                                 gcgcgcgcATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCg    <  2:752624/140‑1 (MQ=255)

                                                                                                                                  cgcgcgCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGc           <  1:363567/132‑1 (MQ=255)

                                                                                                                                  cgcgcgCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGc           >  2:363567/1‑132 (MQ=255)

                                                                                                                                  cgcgcgCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCgg   >  1:1221578/1‑140 (MQ=255)

                                                                                                                                  cgcgcgCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCgg   >  2:581151/1‑140 (MQ=255)

                                                                                                                                   gcgcgcATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGt  >  1:462290/1‑140 (MQ=255)

CGATAAATAGTGTGGGCGGCAGTTTTAGCCTTTTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGT  >  NC_000913/2924878‑2925148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`