Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	155,212	T→C	D72G (GAC→GGC)	htrE ←	putative fimbrial usher protein HtrE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	155,212	0	T	C	100.0%	253.7 / NA	74	D72G (GAC→GGC)	htrE	putative fimbrial usher protein HtrE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/43);  total (31/43)

CAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGC  >  NC_000913/155076‑155348                                                                                                                                         |                                                                                                                                         cAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGt                                                                                                                                       >  2:962575/1‑140 (MQ=255)  aGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGtt                                                                                                                                      <  2:476578/140‑1 (MQ=255)  aGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTGCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGtt                                                                                                                                      <  1:864959/140‑1 (MQ=255)      cGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACa                                                                                                                                  <  2:353157/140‑1 (MQ=255)      cGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACa                                                                                                                                  <  2:557610/140‑1 (MQ=255)          tttttCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATaaa                                                                                                                              <  1:772207/140‑1 (MQ=255)               cGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTg                                                                                                                         <  2:1145631/140‑1 (MQ=255)                  tatttatATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGAcg                                                                                                                      >  1:656646/1‑140 (MQ=255)                   atttatATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGAcgt                                                                                                                     >  2:244873/1‑140 (MQ=255)                      tataTCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGAcgtcgt                                                                                                                  <  2:572999/140‑1 (MQ=255)                             ggAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGTCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACAcc                                                                                                           <  2:850025/140‑1 (MQ=255)                              gagaATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAaca                                                                                                             >  2:1332924/1‑137 (MQ=255)                              gagaATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAaca                                                                                                             <  1:1332924/137‑1 (MQ=255)                                gaATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCgg                                                                                                        <  2:289698/140‑1 (MQ=255)                                     aaTATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAg                                                                                                   <  2:1026769/140‑1 (MQ=255)                                        atGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCgg                                                                                                >  1:497895/1‑140 (MQ=255)                                        atGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCgg                                                                                                >  1:870307/1‑140 (MQ=255)                                         tGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCggg                                                                                               <  2:751334/140‑1 (MQ=255)                                          gAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCgggg                                                                                              <  1:737372/140‑1 (MQ=255)                                           aaaCTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTGTAGCGGGGt                                                                                             >  2:661545/1‑140 (MQ=255)                                            aaCTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGtt                                                                                            >  1:521833/1‑140 (MQ=255)                                             aCTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTa                                                                                           >  2:300922/1‑140 (MQ=255)                                                 cAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCtt                                                                                       >  2:1220126/1‑140 (MQ=255)                                                 cAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCtt                                                                                       >  2:773191/1‑140 (MQ=255)                                                       aTTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGt                                                                                 <  2:355646/140‑1 (MQ=255)                                                        ttCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTa                                                                                <  2:176243/140‑1 (MQ=255)                                                         tCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTAc                                                                               <  1:244873/140‑1 (MQ=255)                                                         tCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTAc                                                                               <  2:1244094/140‑1 (MQ=255)                                                           tttAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTa                                                                                >  2:1459848/1‑137 (MQ=255)                                                           tttAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTa                                                                                <  1:1459848/137‑1 (MQ=255)                                                                tgtgATACAAGCCTGGGCGCTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTga                                                                        >  1:1272964/1‑140 (MQ=255)                                                                 gtgATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTgag                                                                       <  2:521833/140‑1 (MQ=255)                                                                  tgATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTgaga                                                                      <  2:1131667/140‑1 (MQ=255)                                                                   gATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGAt                                                                     >  2:1439950/1‑140 (MQ=255)                                                                   gATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGAt                                                                     <  1:523320/140‑1 (MQ=255)                                                                    aTACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATc                                                                    <  2:1378284/140‑1 (MQ=255)                                                                    aTACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATc                                                                    <  1:300922/140‑1 (MQ=255)                                                                     tACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCa                                                                   >  2:871125/1‑140 (MQ=255)                                                                       cAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAat                                                                 <  2:870307/140‑1 (MQ=255)                                                                            cTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTgaga                                                                      >  1:1281656/1‑130 (MQ=255)                                                                            cTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTgaga                                                                      <  2:1281656/130‑1 (MQ=255)                                                                            cTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAg                                                            >  2:1159074/1‑140 (MQ=255)                                                                             tGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGa                                                           <  2:850854/140‑1 (MQ=255)                                                                             tGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGa                                                           >  1:964710/1‑140 (MQ=255)                                                                              gggCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGAt                                                          <  2:1469670/140‑1 (MQ=255)                                                                               ggCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATg                                                         >  2:265406/1‑140 (MQ=255)                                                                               ggCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATg                                                         >  2:556837/1‑140 (MQ=255)                                                                               ggCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATg                                                         >  2:1250171/1‑140 (MQ=255)                                                                                    tCTTTTTTCCTTCAATTGCGACAAATGTACTACTTTGGTTAATGATTGGTTGGCCGCTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTc                                                    >  2:1408719/1‑140 (MQ=255)                                                                                    tCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCa                                                                   <  1:1105266/125‑1 (MQ=255)                                                                                    tCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCa                                                                   >  2:1105266/1‑125 (MQ=255)                                                                                    tCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTc                                                    <  2:1272964/140‑1 (MQ=255)                                                                                    tCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTc                                                    >  2:682095/1‑140 (MQ=255)                                                                                      ttttttCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTg                                                  <  2:1147675/140‑1 (MQ=255)                                                                                        ttttCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGcc                                                <  2:1336487/140‑1 (MQ=255)                                                                                         tttCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGccc                                               <  1:1159074/140‑1 (MQ=255)                                                                                          ttCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGcccc                                              <  1:1250171/140‑1 (MQ=255)                                                                                                 aaTTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGa                                       <  1:1220126/140‑1 (MQ=255)                                                                                                 aaTTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGa                                       <  1:871125/140‑1 (MQ=255)                                                                                                 aaTTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGa                                       <  1:682095/140‑1 (MQ=255)                                                                                                          aaaTGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGt                              <  1:1358183/140‑1 (MQ=255)                                                                                                           aaTGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTc                             >  2:536746/1‑140 (MQ=255)                                                                                                            aTGTAATACTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCa                            <  2:199454/140‑1 (MQ=255)                                                                                                                    cTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAAc                    <  2:497895/140‑1 (MQ=255)                                                                                                                    cTTTGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAAc                    <  1:244590/140‑1 (MQ=255)                                                                                                                     tttGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAAc                    >  1:614549/1‑139 (MQ=255)                                                                                                                     tttGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAAc                    <  2:614549/139‑1 (MQ=255)                                                                                                                       tGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAg                 >  2:530726/1‑140 (MQ=255)                                                                                                                       tGGTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAg                 >  1:1039974/1‑140 (MQ=255)                                                                                                                         gTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGtt               >  2:1198884/1‑140 (MQ=255)                                                                                                                         gTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGtt               <  2:508032/140‑1 (MQ=255)                                                                                                                         gTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGtt               >  1:968179/1‑140 (MQ=255)                                                                                                                         gTTAATGATTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGtt               >  2:910021/1‑140 (MQ=255)                                                                                                                                aTTGGTTGGCCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACt        <  1:962575/140‑1 (MQ=255)                                                                                                                                   ggttggcCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGaa     >  1:84404/1‑140 (MQ=255)                                                                                                                                     ttggcCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAg   <  1:536746/140‑1 (MQ=255)                                                                                                                                     ttggcCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAg   <  1:1439950/140‑1 (MQ=255)                                                                                                                                      tggcCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGc  <  2:246588/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         CAGAACGGCTTTTTCGTTATTTATATCGGGAGAATTAATATGAAACTGCAATAAATTCTTTAATGTGATACAAGCCTGGGCGTTCTTTTTTCCTTCAATTGCGACAAATGTAATACTTTGGTTAATGATTGGTTGGTCGTTTACATAAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGC  >  NC_000913/155076‑155348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑