Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,068,157	T→C	intergenic (‑77/+16)	yggE ← / ← argO	uncharacterized protein YggE/L‑arginine exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,068,157	0	T	C	100.0%	275.3 / NA	79	intergenic (‑77/+16)	yggE/argO	uncharacterized protein YggE/L‑arginine exporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (38/41);  total (38/41)

TGCGCTGCCATCCCGCTAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGTGCGCAGACG  >  NC_000913/3068022‑3068292                                                                                                                                        |                                                                                                                                        tGCGCTGCCATCCCGCTAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCa                                                                                                                                     >  1:1029383/1‑140 (MQ=255) tGCGCTGCCATCCCGCTAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCa                                                                                                                                     >  2:749652/1‑140 (MQ=255)   cgcTGCCATCCCGCTAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATc                                                                                                                                   >  2:1334852/1‑140 (MQ=255)   cgcTGCCATCCCGCTAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATc                                                                                                                                   >  1:834363/1‑140 (MQ=255)   cgcTGCCATCCCGCTAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATc                                                                                                                                   >  1:82669/1‑140 (MQ=255)      tGCCATCCCGCTAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGa                                                                                                                                <  2:82669/140‑1 (MQ=255)           tCCCGCTAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAgcg                                                                                                                           <  2:360406/140‑1 (MQ=255)           tCCCGCTAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAgcg                                                                                                                           <  2:271950/140‑1 (MQ=255)           tCCCGCTAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAgcg                                                                                                                           <  2:723294/140‑1 (MQ=255)             ccGCTAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGcc                                                                                                                         <  2:1460987/140‑1 (MQ=255)             ccGCTAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGcc                                                                                                                         >  2:1150389/1‑140 (MQ=255)             ccGCTAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGcc                                                                                                                         <  2:834363/140‑1 (MQ=255)                cTAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTaa                                                                                                                      <  1:1195290/140‑1 (MQ=255)                 tAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAAc                                                                                                                     <  1:1334852/140‑1 (MQ=255)                  aaTACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATa                                                                                                                              >  2:664025/1‑130 (MQ=255)                  aaTACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATa                                                                                                                              <  1:664025/130‑1 (MQ=255)                     aCCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGcc                                                                                                                         >  2:842930/1‑132 (MQ=255)                     aCCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGcc                                                                                                                         <  1:842930/132‑1 (MQ=255)                     aCCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGaa                                                                                                                 <  1:786584/140‑1 (MQ=255)                         aTTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAg                                                                                                             >  2:575264/1‑140 (MQ=255)                                 cgccAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGc                                                                                                     >  1:881716/1‑140 (MQ=255)                                 cgccAGGGCAATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGc                                                                                                     <  1:1399341/140‑1 (MQ=255)                                   ccAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGc                                                                                                           >  2:183994/1‑132 (MQ=255)                                   ccAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGc                                                                                                           <  1:183994/132‑1 (MQ=255)                                   ccAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCt                                                                                                          <  1:343380/133‑1 (MQ=255)                                   ccAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCt                                                                                                          >  2:343380/1‑133 (MQ=255)                                    cAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATg                                                                                                  <  1:62647/140‑1 (MQ=255)                                      gggCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAg                                                                                                <  2:1013102/140‑1 (MQ=255)                                           aTAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCtt                                                                                                         >  2:362884/1‑126 (MQ=255)                                           aTAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCtt                                                                                                         <  1:362884/126‑1 (MQ=255)                                            tAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATAc                                                                                          <  2:831479/140‑1 (MQ=255)                                             aaCTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATAcc                                                                                         >  2:857974/1‑140 (MQ=255)                                                 ttGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATAc                                                                                          <  1:1393228/135‑1 (MQ=255)                                                 ttGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATAc                                                                                          >  2:1393228/1‑135 (MQ=255)                                                 ttGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGtct                                                                                     <  2:933446/140‑1 (MQ=255)                                                       ttCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGcc                                                                               <  2:462958/140‑1 (MQ=255)                                                         cACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAg                                                                             >  2:290876/1‑140 (MQ=255)                                                          aCGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGc                                                                            >  1:740253/1‑140 (MQ=255)                                                                   tctcCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTGTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTATCGCCAGCTGCAAGGCa                                                                   >  2:1316816/1‑140 (MQ=255)                                                                     tcCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAAt                                                                 >  1:296048/1‑140 (MQ=255)                                                                     tcCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAAt                                                                 >  2:1146583/1‑140 (MQ=255)                                                                      ccGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATa                                                                <  2:685538/140‑1 (MQ=255)                                                                        gtgtTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATaaa                                                              <  1:930868/140‑1 (MQ=255)                                                                        gtgtTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATaaa                                                              <  2:296048/140‑1 (MQ=255)                                                                        gagtTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATaaa                                                              <  2:1368074/138‑1 (MQ=255)                                                                          gtTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAAcc                                                            >  1:1223699/1‑140 (MQ=255)                                                                          gtTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAAcc                                                            >  1:78516/1‑140 (MQ=255)                                                                             aaTTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACcaca                                                         <  2:740253/140‑1 (MQ=255)                                                                             aaTTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACcaca                                                         >  2:652978/1‑140 (MQ=255)                                                                               ttGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATa                                                       >  1:318740/1‑140 (MQ=255)                                                                                  aCTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAaca                                                    <  1:652978/140‑1 (MQ=255)                                                                                   cTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAacac                                                   >  2:382946/1‑140 (MQ=255)                                                                                   cTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAacac                                                   <  2:1223699/140‑1 (MQ=255)                                                                                      gCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATc                                                >  2:173595/1‑140 (MQ=255)                                                                                       cccAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATcc                                               <  2:1029383/140‑1 (MQ=255)                                                                                       cccAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATcc                                               >  1:221090/1‑140 (MQ=255)                                                                                       cccAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATcc                                               <  1:1077387/140‑1 (MQ=255)                                                                                               tCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCa                                       <  1:290876/140‑1 (MQ=255)                                                                                                 aGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAacac                                                   <  2:120802/126‑1 (MQ=255)                                                                                                 aGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAacac                                                   >  1:120802/1‑126 (MQ=255)                                                                                                  ggACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGAt                                    <  2:318740/140‑1 (MQ=255)                                                                                                  ggACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGAt                                    <  1:857974/140‑1 (MQ=255)                                                                                                      ctctGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGAt                                >  1:1471346/1‑140 (MQ=255)                                                                                                       tctGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATa                               <  2:1471346/140‑1 (MQ=255)                                                                                                        ctGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATaa                              >  2:1357517/1‑140 (MQ=255)                                                                                                        ctGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATaa                              >  2:521570/1‑140 (MQ=255)                                                                                                        ctGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATaa                              >  2:527414/1‑140 (MQ=255)                                                                                                               cAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCtg                       <  1:382946/140‑1 (MQ=255)                                                                                                                  gCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGc                    >  1:1039564/1‑140 (MQ=255)                                                                                                                   cTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCt                   >  2:468859/1‑140 (MQ=255)                                                                                                                   cTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCt                   >  1:761837/1‑140 (MQ=255)                                                                                                                   cTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCt                   >  1:348728/1‑140 (MQ=255)                                                                                                                    ttAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCtt                  <  2:1039564/140‑1 (MQ=255)                                                                                                                       gcgcCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTg               <  1:1357517/140‑1 (MQ=255)                                                                                                                        cgcCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGc              >  2:1415757/1‑140 (MQ=255)                                                                                                                             ctGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTg               >  2:108539/1‑134 (MQ=255)                                                                                                                             ctGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTg               <  1:108539/134‑1 (MQ=255)                                                                                                                                    aTTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGTGCGCAGAcg  <  1:949109/140‑1 (MQ=255)                                                                                                                                    aTTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGTGCGCAGAcg  <  1:749652/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        TGCGCTGCCATCCCGCTAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGTGCGCAGACG  >  NC_000913/3068022‑3068292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑