Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,207,828	G→A	W153* (TGG→TAG)	ttdB →	L(+)‑tartrate dehydratase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,207,828	0	G	A	100.0%	229.9 / NA	65	W153* (TGG→TAG)	ttdB	L(+)‑tartrate dehydratase subunit beta
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/31);  total (34/31)

CTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCA  >  NC_000913/3207692‑3207963                                                                                                                                         |                                                                                                                                        cTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTAGGt                                                                                                                                      >  2:1207179/1‑140 (MQ=255)      cGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTAGGTCTGcc                                                                                                                                 <  2:829667/140‑1 (MQ=255)      cGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTAGGTCTGcc                                                                                                                                 <  1:1207179/140‑1 (MQ=255)            agGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTAGGTCTGCCGGGTCa                                                                                                                           <  2:245452/140‑1 (MQ=255)                      aaaTTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCgg                                                                                                                 <  1:946419/140‑1 (MQ=255)                          tCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCg                                                                                                             <  1:770715/140‑1 (MQ=255)                           cAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGc                                                                                                            <  1:307350/140‑1 (MQ=255)                               gcgcTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGAt                                                                                                        <  1:1056510/140‑1 (MQ=255)                               gcgcTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGAt                                                                                                        <  1:977771/140‑1 (MQ=255)                                  cTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGt                                                                                                     >  1:151372/1‑140 (MQ=255)                                     cATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGtctc                                                                                                  <  2:179928/140‑1 (MQ=255)                                       tgtgATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTAGGTCCGCCGGGTCAAAGACTTCGGCCCGCTGATTGTCTCTa                                                                                                <  2:784606/140‑1 (MQ=255)                                             tttCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATa                                                                                          <  1:1002124/140‑1 (MQ=255)                                               tCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATAcc                                                                                        >  1:19895/1‑140 (MQ=255)                                                cccGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATAccc                                                                                       >  2:1123712/1‑140 (MQ=255)                                                 ccGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCa                                                                                      <  2:725915/140‑1 (MQ=255)                                                 ccGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCa                                                                                      >  1:1376889/1‑140 (MQ=255)                                                     cAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCa                                                                                      <  2:1106954/136‑1 (MQ=255)                                                     cAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCa                                                                                      >  1:1106954/1‑136 (MQ=255)                                                        gCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGcaac                                                                               >  2:156142/1‑140 (MQ=255)                                                               ggTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGa                                                                        <  1:1402167/140‑1 (MQ=255)                                                                    tGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATGCCCACGGCAACAACCTGATAGcc                                                                   >  1:860082/1‑140 (MQ=255)                                                                      gcggcAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGa                                                                 >  1:1116377/1‑140 (MQ=255)                                                                           aaCCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACa                                                            >  2:161101/1‑140 (MQ=255)                                                                           aaCCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACa                                                            >  1:500331/1‑140 (MQ=255)                                                                                       agagATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCg                                                >  2:21093/1‑140 (MQ=255)                                                                                          gaTTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCg                                             <  1:21093/140‑1 (MQ=255)                                                                                           aTTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGa                                            >  1:975247/1‑140 (MQ=255)                                                                                           aTTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGa                                            >  1:438386/1‑140 (MQ=255)                                                                                            ttGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGaa                                           >  1:1154538/1‑140 (MQ=255)                                                                                            ttGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGaa                                           <  1:1266999/140‑1 (MQ=255)                                                                                            ttGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGaa                                           >  1:999536/1‑140 (MQ=255)                                                                                             tGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAAc                                          >  2:884196/1‑140 (MQ=255)                                                                                             tGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAAc                                          >  2:749015/1‑140 (MQ=255)                                                                                             tGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAAc                                          >  1:474035/1‑140 (MQ=255)                                                                                               aagaagTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAAcgc                                        <  2:1154538/140‑1 (MQ=255)                                                                                                agaagTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAAcgcc                                       >  1:407324/1‑140 (MQ=255)                                                                                                   agTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCcgcg                                    <  2:407324/140‑1 (MQ=255)                                                                                                    gTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGaa                                           <  2:312519/132‑1 (MQ=255)                                                                                                    gTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGaa                                           >  1:312519/1‑132 (MQ=255)                                                                                                    gTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGa                                   >  1:166592/1‑140 (MQ=255)                                                                                                       cACTGGACAGAGCTCGGAATGCCGGAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATcc                                <  2:1116377/140‑1 (MQ=255)                                                                                                          tGGACAGAGCTCGGAATGCCGGAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCAt                             <  1:1123712/140‑1 (MQ=255)                                                                                                            gACAGAGCTCGGAATGCCGGAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCg                           >  1:1088653/1‑140 (MQ=255)                                                                                                            gACAGAGCTCGGAATGCCGGAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCg                           <  2:438386/140‑1 (MQ=255)                                                                                                            gACAGAGCTCGGAATGCCGGAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCg                           >  1:981137/1‑140 (MQ=255)                                                                                                                gagCTCGGAATGCCGGAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGa                       <  2:151372/140‑1 (MQ=255)                                                                                                                 agCTCGGAATGCCGGAGTCACTGTAGGTCTGTCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGaa                      >  2:1393424/1‑140 (MQ=255)                                                                                                                 agCTCGGAATGCCGGAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGaa                      >  1:419534/1‑140 (MQ=255)                                                                                                                  gCTCGGAATGCCGGAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAag                     >  2:1459085/1‑140 (MQ=255)                                                                                                                   cTCGGAATGCCGGAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCa                              >  1:572912/1‑130 (MQ=255)                                                                                                                   cTCGGAATGCCGGAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCa                              <  2:572912/130‑1 (MQ=255)                                                                                                                      ggAATGCCGGAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGAt                 >  2:445856/1‑140 (MQ=255)                                                                                                                      ggAATGCCGGAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGAt                 >  1:1462874/1‑140 (MQ=255)                                                                                                                      ggAATGCCGGAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGAt                 >  1:926483/1‑140 (MQ=255)                                                                                                                      ggAATGCCGGAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGAt                 >  2:1218417/1‑140 (MQ=255)                                                                                                                           gCCGGAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCg            >  2:150782/1‑140 (MQ=255)                                                                                                                             cGGAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATGCCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAg          <  2:860082/140‑1 (MQ=255)                                                                                                                             cGGAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAg          >  2:680571/1‑140 (MQ=255)                                                                                                                              ggAGTCACTGTAGGTCTGTCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGc         <  1:1393424/140‑1 (MQ=255)                                                                                                                              ggAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGc         <  1:680571/140‑1 (MQ=255)                                                                                                                              ggAGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGCTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGc         <  2:1376889/140‑1 (MQ=255)                                                                                                                                aGTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCAc       <  2:419534/140‑1 (MQ=255)                                                                                                                                 gTCACTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACg      <  2:474035/140‑1 (MQ=255)                                                                                                                                     cTGTAGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCa  <  2:1088653/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        CTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCA  >  NC_000913/3207692‑3207963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑