Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,237,712	G→A	Q134Q (CAG→CAA)	ygjR →	putative oxidoreductase YgjR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,237,712	0	G	A	100.0%	227.4 / NA	64	Q134Q (CAG→CAA)	ygjR	putative oxidoreductase YgjR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/41);  total (23/41)

ATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGCGCGGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCAACACCTTTAATCCGGCATTCTCTAACGGTTCAATTATGGATA  >  NC_000913/3237576‑3237848                                                                                                                                         |                                                                                                                                         aTGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGCGCGGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAAgcg                                                                                                                                       <  1:544822/140‑1 (MQ=255)      aTTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGCGCGGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAAGCGCTGcc                                                                                                                                  >  1:343320/1‑140 (MQ=255)       tttGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGCGCGGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAAGCGCTGCCg                                                                                                                                 <  2:400803/140‑1 (MQ=255)       tttGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGAGGCCGCCATTGCCTGTGCGCGGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAAGCGCTGCCg                                                                                                                                 <  1:1102333/140‑1 (MQ=255)              gaAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGCGCGGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAAGCGCTGCCGAAAGTCg                                                                                                                          <  2:1019208/140‑1 (MQ=255)              gaAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGCGCGGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAAGCGCTGCCGAAAGTCg                                                                                                                          >  2:834488/1‑140 (MQ=255)                 aCCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGCGCGGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAAGCGCTGCCGAAAGTCGGCa                                                                                                                       >  1:1108157/1‑140 (MQ=255)                  ccGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGCGCGGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAAGCGCTGCCGAAAGTCGGCaa                                                                                                                      <  2:1015059/140‑1 (MQ=255)                     cTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGCGCGGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAAGCGCTGCCGAAAGTCGGCAAACt                                                                                                                   >  2:1100377/1‑140 (MQ=255)                          gTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGCGCGGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAAGCGCTGCCGAAAGTCGGCAAACTGCGGa                                                                                                              <  1:84533/140‑1 (MQ=255)                             gAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGCGCGGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAg                                                                                                           <  2:823117/140‑1 (MQ=255)                              aaTCTGGCGGAAGTGGATGCCGCCATTGCCTGTGCGCGGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGt                                                                                                          <  2:1160944/140‑1 (MQ=255)                              aaTCTGGCGGAAGTGGATGCCGCCATTGCCTGTGCGCGGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGt                                                                                                          <  2:143067/140‑1 (MQ=255)                               aTCTGGCGGAAGTGGATGCCGCCATTGCCTGTGCGCGGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTc                                                                                                         <  1:903915/140‑1 (MQ=255)                                       gAAGTGGATGCCGCCATTGCCTGTGCGCGGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCaa                                                                                                 <  1:1307071/140‑1 (MQ=255)                                         ggTGAATGCCGCCATTGCCTGTGCGGGGAAAAATCAGGTGTTGCTTTTTGGGGCATTAAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACt                                                                                               <  1:376296/139‑1 (MQ=255)                                         aGTGGATGCCGCCATTGCCTGTGCGCGGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACt                                                                                               <  1:1076133/140‑1 (MQ=255)                                           tGGATGCCGCCATTGCCTGTGCGCGGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTAt                                                                                             <  1:1464550/140‑1 (MQ=255)                                           tGGATGCCGCCATTGCCTGTGCGCGGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTAt                                                                                             <  1:834488/140‑1 (MQ=255)                                              aTGCCGCCATTGCCTGTGCGCGGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTAt                                                                                             >  2:1372552/1‑137 (MQ=255)                                              aTGCCGCCATTGCCTGTGCGCGGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTAt                                                                                             <  1:1372552/137‑1 (MQ=255)                                                  cgccATTGCCTGTGCGCGGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGt                                                                                      >  1:438262/1‑140 (MQ=255)                                                  cgccATTGCCTGTGCGCGGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGt                                                                                      >  2:948548/1‑140 (MQ=255)                                                        tGCCTGTGCGCGGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCt                                                                                >  2:1386551/1‑140 (MQ=255)                                                             gtgCGCGGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCgg                                                                           >  1:324582/1‑140 (MQ=255)                                                              tgCGCGGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGt                                                                          >  1:535849/1‑140 (MQ=255)                                                              tgCGCGGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGt                                                                          >  1:948689/1‑140 (MQ=255)                                                               gcgcGGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTTCGCCAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCt                                                                                <  2:892909/133‑1 (MQ=255)                                                               gcgcGGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCt                                                                                >  1:892909/1‑133 (MQ=255)                                                               gcgcGGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTa                                                                         <  2:1072469/140‑1 (MQ=255)                                                                cgcgGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTAt                                                                        <  1:987907/140‑1 (MQ=255)                                                                cgcgGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTAt                                                                        <  1:190200/140‑1 (MQ=255)                                                                cgcgGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTAt                                                                        <  2:324582/140‑1 (MQ=255)                                                                    ggAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAAc                                                                    <  1:1100377/140‑1 (MQ=255)                                                                      aaaaTCAGGTGGTGCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGt                                                                  <  1:1386551/140‑1 (MQ=255)                                                                          tCAGGTGGTGCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTAcc                                                              <  1:1126191/140‑1 (MQ=255)                                                                              gtggtgCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGa                                                          <  1:35972/140‑1 (MQ=255)                                                                                  tgCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAAc                                                                    >  2:212496/1‑126 (MQ=255)                                                                                  tgCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAAc                                                                    <  1:212496/126‑1 (MQ=255)                                                                                  tgCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGt                                                      >  2:466298/1‑140 (MQ=255)                                                                                        atgtggCAATTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGATATCAACGTTACCTGGATGGTGAGAAt                                                >  2:259301/5‑140 (MQ=255)                                                                                           aGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATccc                                             <  1:466298/140‑1 (MQ=255)                                                                                            ggCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCa                                            >  1:984499/1‑140 (MQ=255)                                                                                             gCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCaa                                           <  2:438262/140‑1 (MQ=255)                                                                                                 ttAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCAACAcc                                       >  1:760200/1‑140 (MQ=255)                                                                                                       ccGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCAACACCTTTAAt                                 <  2:1108157/140‑1 (MQ=255)                                                                                                           ctgcctgcCGAACTTTCATTTGTTGCGCCAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCAACACCTTTAATCCgg                             <  2:610501/140‑1 (MQ=255)                                                                                                           ctgcctgcCGAACTTTCATTTGTTGCGCCAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCAACACCTTTAATCCgg                             <  2:381110/140‑1 (MQ=255)                                                                                                           ctgcctgcCGAACTTTCATTTGTTGCGCCAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCAACACCTTTAATCCgg                             <  1:240937/140‑1 (MQ=255)                                                                                                           ctgcctgcCGAACTTTCATTTGTTGCGCCAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCAACACCTTTAATCCgg                             <  1:972544/140‑1 (MQ=255)                                                                                                           ctgcctgcCGAACTGTCATTTGTTGTGCCAAGCGCTGCGGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAGCTATTGCCAGTATTCCTCGCGGTATCAACGTTGCCTGGATGGTGAGAATCCCAACACCTTTAATCCgg                             <  2:95170/140‑1 (MQ=255)                                                                                                             gcctgccGAACTTTCATTTGTTGCGCCAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGATATCAACGTTACCTGGATGGTGAGAATCCCAACACCTTTAATCCGGCa                           <  1:259301/140‑1 (MQ=255)                                                                                                               ctgccGAACTTTCATTTGTTGCGCCAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCAACACCTTTAATCCGGCAtt                         >  1:1338111/1‑140 (MQ=255)                                                                                                                 gccGAACTTTCATTTGTTGCGCCAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCAACACCTTTAATCCgg                             <  2:1465756/134‑1 (MQ=255)                                                                                                                 gccGAACTTTCATTTGTTGCGCCAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCAACACCTTTAATCCgg                             >  1:1465756/1‑134 (MQ=255)                                                                                                                   cGAACTTTCATTTGTTGCGCCAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCAACACCTTTAATCCGGCATTctct                     <  2:1338111/140‑1 (MQ=255)                                                                                                                   cGAACTTTCATTTGTTGCGCCAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCAACACCTTTAATCCGGCATTctct                     <  1:1148426/140‑1 (MQ=255)                                                                                                                     aaCTTTCTTTTGTTGCGCCAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCAACACCTTTAATCCGGCATTCTCTaa                   >  2:1077657/1‑140 (MQ=255)                                                                                                                          tCATTTGTTGCGCCAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCAACACCTTTAATCCGGCATTCTCTAACGGtt              <  2:343320/140‑1 (MQ=255)                                                                                                                            aTTTGTTGCGCCAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCAACACCTTTAATCCGGCATTCTCTAACGGTTCa            >  1:1134901/1‑140 (MQ=255)                                                                                                                                    cgcCAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCAACACCTTTAATCCGGCATTctct                     >  2:1281671/1‑123 (MQ=255)                                                                                                                                    cgcCAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCAACACCTTTAATCCGGCATTctct                     <  1:1281671/123‑1 (MQ=255)                                                                                                                                    cgcCAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCAACACCTTTAATCCGGCATTCTCTAACGGTTCAATTATGGa    >  1:626976/1‑140 (MQ=255)                                                                                                                                      ccAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCAACACCTTTAATCCGGCATTCTCTAACGGTTCAATTATGGata  <  2:1134901/140‑1 (MQ=255)                                                                                                                                      ccAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCAACACCTTTAATCCGGCATTCTCTAACGGTTCAATTATGGata  >  2:808173/1‑140 (MQ=255)                                                                                                                                      ccAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCAACACCTTTAATCCGGCATTCTCTAACGGTTCAATTATGGata  <  1:808173/140‑1 (MQ=255)                                                                                                                                      ccAAGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCAACACCTTTAATCCGGCATTCTCTAACGGTTCAATTATGGata  <  1:883038/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         ATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGCGCGGGAAAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCAACACCTTTAATCCGGCATTCTCTAACGGTTCAATTATGGATA  >  NC_000913/3237576‑3237848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑