Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	177,927	A→G	V179A (GTA→GCA)	btuF ←	vitamin B12 ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	177,927	0	A	G	100.0%	205.6 / NA	60	V179A (GTA→GCA)	btuF	vitamin B12 ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/33);  total (27/33)

GATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGCGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGG  >  NC_000913/177791‑178059                                                                                                                                         |                                                                                                                                     gATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGCGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTGCCt                                                                                                                                   >  1:288785/1‑140 (MQ=255)      tAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGCGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTGCCTGGTTc                                                                                                                              <  1:87728/140‑1 (MQ=255)            tttGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGCGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTGCCTGGTTCTGAATc                                                                                                                        <  2:1067744/140‑1 (MQ=255)             ttGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGCGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTGCCTGGTTCTGAATCg                                                                                                                       >  2:1075635/1‑140 (MQ=255)             ttGGTCCGGTCCGCCTGTAATGACAATCGCCAGTGGCGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTGCCTGGTTCTGAATCg                                                                                                                       >  2:338184/1‑140 (MQ=255)                      tccgCCTGTAATGACAATCGCCTGTGGCGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTGCCTGGTTCTGAATCGACTCTTTTc                                                                                                              <  1:530184/140‑1 (MQ=255)                      tccgCCTGTAATGACAATCGCCTGTGGCGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTGCCTGGTTCTGAATCGACTCTTTTc                                                                                                              <  1:1135123/140‑1 (MQ=255)                        cgCCTGTAATGACAATCGCCTGTGGCGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTGCCTGGTTCTGAATCGACTCTTTTCCa                                                                                                            <  2:288785/140‑1 (MQ=255)                                   aCAATCGCCTGTGGCGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTGCCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATgg                                                                                                 >  2:192183/1‑140 (MQ=255)                                        cGCCTGTGGCGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTGCCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGAt                                                                                            >  1:1418366/1‑140 (MQ=255)                                        cGCCTGTGGCGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTGCCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGAt                                                                                            <  2:1418366/140‑1 (MQ=255)                                          ccTGTGGCGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTGCCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTa                                                                                          >  2:947176/1‑140 (MQ=255)                                                  gAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTGCCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGaa                                                                                  <  1:33179/140‑1 (MQ=255)                                                   aGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTGCCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAAt                                                                                 >  1:97977/1‑140 (MQ=255)                                                               ccTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTGCCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAaca                                                                     <  1:933240/140‑1 (MQ=255)                                                                  gTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTGCCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAaca                                                                     <  1:1072684/137‑1 (MQ=255)                                                                  gTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTGCCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAaca                                                                     >  2:1072684/1‑137 (MQ=255)                                                                  gTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTGCCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGt                                                                  >  2:153567/1‑140 (MQ=255)                                                                  gTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTGCCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGt                                                                  >  2:171382/1‑140 (MQ=255)                                                                  gTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTGCCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGt                                                                  >  1:1168967/1‑140 (MQ=255)                                                                  gTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTGCCTGGTTCTGAATCGACTCTTTTCCACTGGCAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGt                                                                  >  1:918254/1‑140 (MQ=255)                                                                       cgGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTGCCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGtttttt                                                             <  1:171382/140‑1 (MQ=255)                                                                       cgGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTGCCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGtttttt                                                             >  1:10171/1‑140 (MQ=255)                                                                        ggCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTGCCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTTTa                                                            <  1:192183/140‑1 (MQ=255)                                                                         gCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAAC‑TCGAGTGCCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTTTAgg                                                          <  1:281737/140‑1 (MQ=255)                                                                          cTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTGCCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTTTAgg                                                          <  1:520082/140‑1 (MQ=255)                                                                          cTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTGCCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTTTAgg                                                          <  1:1075635/140‑1 (MQ=255)                                                                          cTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTGCCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTTTAgg                                                          <  1:338184/140‑1 (MQ=255)                                                                              cTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTGCCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTTTAGGTTTa                                                      <  2:10171/140‑1 (MQ=255)                                                                               ttGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTGCCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTTTAGGTTTAt                                                     <  2:8893/140‑1 (MQ=255)                                                                               ttGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTGCCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTTTAGGTTTAt                                                     <  1:153567/140‑1 (MQ=255)                                                                                    gCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTGCCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTTTAGGTTTATCAGCa                                                >  2:378734/1‑140 (MQ=255)                                                                                        gggAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTGCCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTTTAGGTTTATCAGCATAtt                                            >  1:417412/1‑140 (MQ=255)                                                                                        gggAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTGCCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTTTAGGTTTATCAGCATAtt                                            >  1:1189743/1‑140 (MQ=255)                                                                                        gggAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTGCCTGGTTCTGAATCGACTCTTTTCCACTGGCAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTTTAGGTTTATCAGCATAtt                                            <  2:918254/140‑1 (MQ=255)                                                                                         ggAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTGCCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTTTAGGTTTATCAGCATATTg                                           >  2:1053378/1‑140 (MQ=255)                                                                                          gAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTGCCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTTTAGGTTTATCAGCATATTgc                                          <  2:1189743/140‑1 (MQ=255)                                                                                          gAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTGCCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTTTAGGTTTATCAGCATATTgc                                          <  2:417412/140‑1 (MQ=255)                                                                                          gAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTGCCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTTTAGGTTTATCAGCATATTgc                                          <  2:97977/140‑1 (MQ=255)                                                                                              ccGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTGCCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTTTAGGTTTATCAGCATATTGCGCtt                                      >  1:867014/1‑140 (MQ=255)                                                                                                 gCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTGCCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTTTAGGTTTATCAGCATATTGCGCTTTCa                                   <  2:1168967/140‑1 (MQ=255)                                                                                                   tGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTGCCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAAt                                 <  2:235838/140‑1 (MQ=255)                                                                                                   tGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTGCCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAAt                                 >  1:718571/1‑140 (MQ=255)                                                                                                   tGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTGCCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAAt                                 >  1:235838/1‑140 (MQ=255)                                                                                                      cTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTGCCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTTTATGTTTATCAGCATATTGCGCTTTCAATTgc                              <  2:1264208/140‑1 (MQ=255)                                                                                                       tttAAAGATGTTTTCTCCGCCACAAACTTCGAGTGCCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTgcg                             <  1:371340/140‑1 (MQ=255)                                                                                                          aaaGATGTTTTCTCCGCCACAAACTTCGAGTGCCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGt                          <  1:367492/140‑1 (MQ=255)                                                                                                          aaaGATGTTTTCTCCGCCACAAACTTCGAGTGCCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGt                          <  1:1299917/140‑1 (MQ=255)                                                                                                               tGTTTTCTCCGCCACAAACTTCGAGTGCCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTTTAGGTTTATCAGCATATTGCGCTTTCa                                   >  2:1356124/1‑126 (MQ=255)                                                                                                               tGTTTTCTCCGCCACAAACTTCGAGTGCCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTTTAGGTTTATCAGCATATTGCGCTTTCa                                   <  1:1356124/126‑1 (MQ=255)                                                                                                               tGTTTTCTCCGCCACAAACTTCGAGTGCCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGa                     >  1:179770/1‑140 (MQ=255)                                                                                                                gTTTTCTCCGCCACAAACTTCGAGTGCCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGAt                    <  2:1228679/140‑1 (MQ=255)                                                                                                                gTTTTCTCCGCCACAAACTTCGAGTGCCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGAt                    >  1:1228679/1‑140 (MQ=255)                                                                                                                 ttttCTCCGCCACAAACTTCGAGTGCCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATc                   >  1:1478102/1‑140 (MQ=255)                                                                                                                     ctcCGCCACAAACTTCGAGTGCCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTgc                              >  1:944712/1‑125 (MQ=255)                                                                                                                     ctcCGCCACAAACTTCGAGTGCCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTgc                              <  2:944712/125‑1 (MQ=255)                                                                                                                     ctcCGCCACAAACTTCGAGTGCCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCcagc               >  1:621601/1‑140 (MQ=255)                                                                                                                       ccGCCACAAACTTCGAGTGCCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCcagcag             >  1:373181/1‑140 (MQ=255)                                                                                                                                aCTTCGAGTGCCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTgcgc    <  2:718571/140‑1 (MQ=255)                                                                                                                                  ttCGAGTGCCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCgg  <  1:947176/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     GATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGCGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGG  >  NC_000913/177791‑178059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑