Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,250,172	G→A	W32* (TGG→TGA)	yqjK →	conserved protein YqjK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,250,172	0	G	A	98.7%	265.7 / ‑4.6	76	W32* (TGG→TGA)	yqjK	conserved protein YqjK
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (29/46);  total (29/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

TGAGTTAGCAAACGATCGGCAGCTGCTCGAGGAGGAGTCCCGTGAGCAGTAAAGTCGAACGTGAACGACGTAAGGCGCAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGGCTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTGCGCTCCTGGGCGCTGGTTGGCAGTAGCGTGATGGCGATCTGGACGATTCGCCATCCTAATATGCTGGTCCGCTG  >  NC_000913/3250036‑3250297                                                                                                                                         |                                                                                                                              tGAGTTAGCAAACGATCGGCAGCTGCTCGAGGAGGAGTCCCGTGAGCAGTAAAGTCGAACGTGAACGACGTAAGGCGCAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTg                                                                                                                            <  1:622823/140‑1 (MQ=255) tGAGTTAGCAAACGATCGGCAGCTGCTCGAGGAGGAGTCCCGTGAGCAGTAAAGTCGAACGTGAACGACGTAAGGCGCAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTg                                                                                                                            <  2:1077867/140‑1 (MQ=255)  gAGTTAGCAAACGATCGGCAGCTGCTCGAGGAGGAGTCCCGTGAGCAGTAAAGTCGAACGTGAACGACGTAAGGCGCAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTgg                                                                                                                           >  2:1111045/1‑140 (MQ=255)  gAGTTAGCAAACGATCGGCAGCTGCTCGAGGAGGAGTCCCGTGAGCAGTAAAGTCGAACGTGAACGACGTAAGGCGCAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTgg                                                                                                                           >  1:174346/1‑140 (MQ=255)       aGCAAACGATCGGCAGCTGCTCGAGGAGGAGTCCCGTGAGCAGTAAAGTCGAACGTGAACGACGTAAGGCGCAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGaca                                                                                                                      >  2:738746/1‑140 (MQ=255)        gCAAACGATCGGCAGCTGCTCGAGGAGGAGTCCCGTGAGCAGTAAAGTCGAACGTGAACGACGTAAGGCGCAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAACGACTGGAGacaa                                                                                                                     <  1:438900/140‑1 (MQ=255)          aaaCGATCGGCAGCTGCTCGAGGAGGAGTCCCGTGAGCAGTAAAGTCGAACGTGAACGACGTAAGGCGCAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGacaaca                                                                                                                   <  2:1028210/140‑1 (MQ=255)           aaCGATCGGCAGCTGCTCGAGGAGGAGTCCCGTGAGCAGTAAAGTCGAACGTGAACGACGTAAGGCGCAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAg                                                                                                                  <  1:387533/140‑1 (MQ=255)           aaCGATCGGCAGCTGCTCGAGGAGGAGTCCCGTGAGCAGTAAAGTCGAACGTGAACGACGTAAGGCGCAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAg                                                                                                                  <  1:289892/140‑1 (MQ=255)            aCGATCGGCAGCTGCTCGAGGAGGAGTCCCGTGAGCAGTAAAGTCGAACGTGAACGACGTAAGGCGCAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAgg                                                                                                                 <  2:1065850/140‑1 (MQ=255)              gATCGGCAGCTGCTCGAGGAGGAGTCCCGTGAGCAGTAAAGTCGAACGTGAACGACGTAAGGCGCAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGgcg                                                                                                               >  2:625520/1‑140 (MQ=255)                  ggCAGCTGCTCGAGGAGGAGTCCCGTGAGCAGTAAAGTCGAACGTGAACGACGTAAGGCGCAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTa                                                                                                           >  2:484131/1‑140 (MQ=255)                      gctgctCGAGGAGGAGTCCCGTGAGCAGTAAAGTCGAACGTGAACGACGTAAGGCGCAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGAt                                                                                                       <  2:387526/140‑1 (MQ=255)                      gctgctCGAGGAGGAGTCCCGTGAGCAGTAAAGTCGAACGTGAACGACGTAAGGCGCAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGAt                                                                                                       <  1:421512/140‑1 (MQ=255)                      gctgctCGAGGAGGAGTCCCGTGAGCAGTAAAGTCGAACGTGAACGACGTAAGGCGCAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGAt                                                                                                       <  1:1111045/140‑1 (MQ=255)                      gctgctCGAGGAGGAGTCCCGTGAGCAGTAAAGTCGAACGTGAACGACGTAAGGCGCAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGAt                                                                                                       <  2:833897/140‑1 (MQ=255)                      gctgctCGAGGAGGAGTCCCGTGAGCAGTAAAGTCGAACGTGAACGACGTAAGGCGCAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGAt                                                                                                       <  2:240286/140‑1 (MQ=255)                      gctgctCGAGGAGGAGTCCCGTGAGCAGTAAAGTCGAACGTGAACGACGTAAGGCGCAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGAt                                                                                                       <  1:1061548/140‑1 (MQ=255)                         gctCGAGGAGGAGTCCCGTGAGCAGTAAAGTCGAACGTGAACGACGTAAGGCGCAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGAtcgt                                                                                                    <  2:1186062/140‑1 (MQ=255)                          cttgaggaggagTCCCGTGAGCAGTAAAGTCGAACGTGAACGACGTAAGGCGCAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACg                                                                                                         >  1:188097/4‑134 (MQ=255)                          cttgaggaggagTCCCGTGAGCAGTAAAGTCGAACGTGAACGACGTAAGGCGCAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACg                                                                                                         <  2:188097/131‑1 (MQ=255)                          ctCGAGGAGGAGTCCCGTGAGCAGTAAAGTCGAACGTGAACGACGTAAGGCGCAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACg                                                                                                         >  1:766327/1‑134 (MQ=255)                          ctCGAGGAGGAGTCCCGTGAGCAGTAAAGTCGAACGTGAACGACGTAAGGCGCAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACg                                                                                                         <  2:766327/134‑1 (MQ=255)                           tCGAGGAGGAGTCCCGTGAGCAGTAAAGTCGAACGTGAACGACGTAAGGCGCAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGAtcgtcg                                                                                                  <  2:313487/140‑1 (MQ=255)                                    agTCCCGTGAGCAGTAAAGTCGAACGTGAACGACGTAAGGCGCAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGATCGTCGCTGGAatat                                                                                         <  1:256854/140‑1 (MQ=255)                                    agTCCCGTGAGCAGTAAAGTCGAACGTGAACGACGTAAGGCGCAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGATCGTCGCTGGAatat                                                                                         >  1:257141/1‑140 (MQ=255)                                     gTCCCGTGAGCAGTAAAGTCGAACGTGAACGACGTAAGGCGCAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGATCGTCGCTGGa                                                                                             <  1:1200577/135‑1 (MQ=255)                                     gTCCCGTGAGCAGTAAAGTCGAACGTGAACGACGTAAGGCGCAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGATCGTCGCTGGa                                                                                             >  2:1200577/1‑135 (MQ=255)                                     gTCCCGTGAGCAGTAAAGTCGAACGCGAACGACGTAAGGCGCAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGGCTGG‑‑‑CAACAGGCGCTTACGATCGTCGCTGGAATAtgctg                                                                                     <  1:1365730/140‑1 (MQ=255)                                        ccGTGAGCAGTAAAGTCGAACGTGAACGACGTAAGGCGCAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGAtcgtcg                                                                                                  >  2:1088650/1‑127 (MQ=255)                                        ccGTGAGCAGTAAAGTCGAACGTGAACGACGTAAGGCGCAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGAtcgtcg                                                                                                  <  1:1088650/127‑1 (MQ=255)                                           tGAGCAGTAAAGTCGAACGTGAACGACGTAAGGCGCAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTa                                                                                  >  1:881392/1‑140 (MQ=255)                                            gAGCAGTAAAGTCGAACGTGAACGACGTAAGGCGCAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTaa                                                                                 <  1:1405390/140‑1 (MQ=255)                                             aGCAGTAAAGTCGAACGTGAACGACGTAAGGCGCAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAg                                                                                >  1:604065/1‑140 (MQ=255)                                             aGCAGTAAAGTCGAACGTGAACGACGTAAGGCGCAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAg                                                                                >  1:1315227/1‑140 (MQ=255)                                                aGTAAAGTCGAACGTGAACGACGTAAGGCGCAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCt                                                                             >  2:548258/1‑140 (MQ=255)                                                 gTAAAGTCGAACGTGAACGACGTAAGGCGCAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTg                                                                            <  2:1315227/140‑1 (MQ=255)                                                 gTAAAGTCGAACGTGAACGACGTAAGGCGCAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTg                                                                            <  2:881392/140‑1 (MQ=255)                                                  tAAAGTCGAACGTGAACGACGTAAGGCGCAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTgc                                                                           >  2:611449/1‑140 (MQ=255)                                                  tAAAGTCGAACGTGAACGACGTAAGGCGCAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTgc                                                                           >  1:1133909/1‑140 (MQ=255)                                                   aaaGTCGAACGTGAACGACGTAAGGCGCAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTgcg                                                                          <  1:1207259/140‑1 (MQ=255)                                                        cGAACGTGAACGACGTAAGGCGCAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTGCGCTCCt                                                                     <  2:293673/140‑1 (MQ=255)                                                          aaCGTGAACGACGTAAGGCGCAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTGCGCTCCTgg                                                                   >  2:562078/1‑140 (MQ=255)                                                            cGTGAACGACGTAAGGCGCAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTGCGCTCCTGGgc                                                                 >  2:422742/1‑140 (MQ=255)                                                             gTGAACGACGTAAGGCGCAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTGCGCTCCTGGgcg                                                                <  2:604065/140‑1 (MQ=255)                                                                aaCGACGTAAGGCGCAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTGCGCTCCTGGGCGCTg                                                             >  2:1364252/1‑140 (MQ=255)                                                                     cgTAAGGCGCAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTGCGCTCCTGGGCGCTGGTTgg                                                        <  1:422742/140‑1 (MQ=255)                                                                       tAAGGCGCAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTGCGCTCCTGGGCGCTGGTTGGCa                                                      >  1:670039/1‑140 (MQ=255)                                                                           gcgcAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTGCGCTCCTGGGCGCTGGTTGGCAGTAg                                                  <  1:625520/140‑1 (MQ=255)                                                                             gcAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTGCGCTCCTGGGCGCTGGTTGGCAGTAGCg                                                <  1:562078/140‑1 (MQ=255)                                                                                 cTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTGCGCTCCTGGGCGCTGGTTGGCAGTAGCGTGAt                                            <  1:697117/140‑1 (MQ=255)                                                                                 cTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTGCGCTCCTGGGCGCTGGTTGGCAGTAGCGTGAt                                            >  1:253917/1‑140 (MQ=255)                                                                                  tGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTGCGCTCCTGGGCGCTGGTTGGCAGTAGCGTGATg                                           <  1:1364252/140‑1 (MQ=255)                                                                                  tGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTGCGCTCCTGGGCGCTGGTTGGCAGTAGCGTGATg                                           <  1:684908/140‑1 (MQ=255)                                                                                      tAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTGCGCTCCTGGGCGCTGGTTGGCAGTAGCGTGATGGCGa                                       >  2:1128159/1‑140 (MQ=255)                                                                                        gCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTGCGCTCCTGGGCGCTGGTTGGCAGTAGCGTGATGGCGATc                                     <  2:881900/140‑1 (MQ=255)                                                                                              tCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTGCGCTCCTGGGCGCTGGTTGGCAGTAGCGTGATGGCGATCTGGACg                               >  1:548002/1‑140 (MQ=255)                                                                                              tCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTGCGCTCCTGGGCGCTGGTTGGCAGTAGCGTGATGGCGATCTGGACg                               <  1:690930/140‑1 (MQ=255)                                                                                                  gCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTGCGCTCCTGGGCGCTGGTTGGCAGTAGCGTGATGGCGATCTGGACGATTc                           >  2:1014470/1‑140 (MQ=255)                                                                                                   caacaaCGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTGCGCTCCTGGGCGCTGGTTGGCAGTAGCGTGATGGCGATCTGGACGATTCg                          >  2:341865/1‑140 (MQ=255)                                                                                                   caacaaCGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTGCGCTCCTGGGCGCTGGTTGGCAGTAGCGTGATGGCGATCTGGACGATTCg                          <  2:174346/140‑1 (MQ=255)                                                                                                   caacaaCGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTGCGCTCCTGGGCGCTGGTTGGCAGTAGCGTGATGGCGATCTGGACGATTCg                          <  2:1133909/140‑1 (MQ=255)                                                                                                   caacaaCGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTGCGCTCCTGGGCGCTGGTTGGCAGTAGCGTGATGGCGATCTGGACGATTCg                          <  1:1455208/140‑1 (MQ=255)                                                                                                   caacaaCGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTGCGCTCCTGGGCGCTGGTTGGCAGTAGCGTGATGGCGATCTGGACGATTCg                          <  1:889673/140‑1 (MQ=255)                                                                                                   caacaaCGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTGCGCTCCTGGGCGCTGGTTGGCAGTAGCGTGATGGCGATCTGGACGATTCg                          <  1:738746/140‑1 (MQ=255)                                                                                                     acaacGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTGCGCTCCTGGGCGCTGGTTGGCAGTAGCGTGATGGCGATCTGGACGATTCGcc                        <  2:548002/140‑1 (MQ=255)                                                                                                      caacGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTGCGCTCCTGGGCGCTGGTTGGCAGTAGCGTGATGGCGATCTGGACGATTCGCCa                       <  1:611449/140‑1 (MQ=255)                                                                                                      caacGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTGCGCTCCTGGGCGCTGGTTGGCAGTAGCGTGATGGCGATCTGGACGATTCGCCa                       <  2:670039/140‑1 (MQ=255)                                                                                                       aacGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTGCGCTCCTGGGCGCTGGTTGGCAGTAGCGTGATGGCGATCTGGACGATTc                           <  2:5735/135‑1 (MQ=255)                                                                                                       aacGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTGCGCTCCTGGGCGCTGGTTGGCAGTAGCGTGATGGCGATCTGGACGATTc                           >  1:5735/1‑135 (MQ=255)                                                                                                       aacGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTGCGCTCCTGGGCGCTGGTTGGCAGTAGCGTGATGGCGATCTGGACGATTCGCCAt                      <  1:484131/140‑1 (MQ=255)                                                                                                         cGGCTGGATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTGCGCTCCTGGGCGCTGGTTGGCAGTAGCGTGATGGCGATCTGGACGATTCGCCATcc                    <  2:268280/140‑1 (MQ=255)                                                                                                              ggATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTGCGCTCCTGGGCGCTGGTTGGCAGTAGCGTGATGGCGATCTGGACGATTCGCCATCCTAata               >  2:228303/1‑140 (MQ=255)                                                                                                               gATCTTTCCGCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTGCGCTCCTGGGCGCTGGTTGGCAGTAGCGTGATGGCGATCTGGACGATTCGCCATCCTAatat              >  1:193737/1‑140 (MQ=255)                                                                                                                        gCCAGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTGCGCTCCTGGGCGCTGGTTGGCAGTAGCGTGATGGCGATCTGGACGATTCGCCATCCTAATATGCTGGTCCg     <  1:1128159/140‑1 (MQ=255)                                                                                                                           aGTCGTCGTGAATGACTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTGCGCTCCTGGGCGCTGGTTGGCAGTAGCGTGATGGCGATCTGGACGATTCGCCATCCTAATATGCTGGTCCGCTg  >  2:425295/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                              TGAGTTAGCAAACGATCGGCAGCTGCTCGAGGAGGAGTCCCGTGAGCAGTAAAGTCGAACGTGAACGACGTAAGGCGCAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGGCTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTGCGCTCCTGGGCGCTGGTTGGCAGTAGCGTGATGGCGATCTGGACGATTCGCCATCCTAATATGCTGGTCCGCTG  >  NC_000913/3250036‑3250297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑