Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,254,967	A→G	T217A (ACG→GCG)	yhaK →	bicupin‑related protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,254,967	0	A	G	100.0%	195.2 / NA	57	T217A (ACG→GCG)	yhaK	bicupin‑related protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/27);  total (30/27)

GAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAA  >  NC_000913/3254834‑3255099                                                                                                                                      |                                                                                                                                     gAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTgcgc                                                                                                                                   >  2:651070/1‑137 (MQ=255) gAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTgcgc                                                                                                                                   <  1:651070/137‑1 (MQ=255)     ttCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTGCGCTGGTTGc                                                                                                                            <  2:1418217/140‑1 (MQ=255)     ttCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTGCGCTGGTTGc                                                                                                                            >  1:832870/1‑140 (MQ=255)                 gggCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTGCGCTGGTTGCCGATTCCCCACt                                                                                                                <  1:1217252/140‑1 (MQ=255)                 gggCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTGCGCTGGTTGCCGATTCCCCACt                                                                                                                <  1:587508/140‑1 (MQ=255)                  ggCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTGCGCTGGTTGCCGATTCCCCACTg                                                                                                               >  1:1165174/1‑140 (MQ=255)                   gCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTGCGCTGGTTGCCGATTCCCCACTgc                                                                                                              >  2:996936/1‑140 (MQ=255)                     cACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTGCGCTGGTTGCCGATTCCCCACTgcgc                                                                                                            <  2:848088/140‑1 (MQ=255)                      aCGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTGCGCTGGTTGCCGATTCCCCACTgcgcg                                                                                                           >  2:323157/1‑140 (MQ=255)                       cgcgcgTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTGCGCTGGTTGCCGATTCCCCACTgcgcgc                                                                                                          >  2:633698/1‑140 (MQ=255)                        gcgcgTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTGCGCTGGTTGCCGATTCCCCACTGCGCGCt                                                                                                         >  2:877580/1‑140 (MQ=255)                        gcgcgTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTGCGCTGGTTGCCGATTCCCCACTGCGCGCt                                                                                                         >  1:181344/1‑140 (MQ=255)                        gcgcgTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTGCGCTGGTTGCCGATTCCCCACTGCGCGCt                                                                                                         >  2:357920/1‑140 (MQ=255)                        gcgcgTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTGCGCTGGTTGCCGATTCCCCACTGCGCGCt                                                                                                         >  1:395348/1‑140 (MQ=255)                             tATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTGCGCTGGTTGCCGATTCCCCACTGCGCGCt                                                                                                         >  2:1340911/1‑135 (MQ=255)                             tATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTGCGCTGGTTGCCGATTCCCCACTGCGCGCt                                                                                                         <  1:1340911/135‑1 (MQ=255)                             tATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTGCGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCt                                                                                                    >  2:313196/1‑140 (MQ=255)                             tATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTGCGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCt                                                                                                    >  2:837503/1‑140 (MQ=255)                               tttGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTGCGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGa                                                                                                  <  1:357920/140‑1 (MQ=255)                                   cAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTGCGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGa                                                                                              <  2:789073/140‑1 (MQ=255)                                   cAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTGCGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGa                                                                                              <  1:877580/140‑1 (MQ=255)                                        gATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTGCGCTGGTTGCCGATTCCCCACTGCGCGCtt                                                                                                        <  1:1144300/125‑1 (MQ=255)                                        gATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTGCGCTGGTTGCCGATTCCCCACTGCGCGCtt                                                                                                        >  2:1144300/1‑125 (MQ=255)                                        gATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTGCGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGa                                                                                                  >  1:436047/1‑131 (MQ=255)                                        gATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTGCGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGa                                                                                                  <  2:436047/131‑1 (MQ=255)                                        gATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTGCGCTGGTTGCCGATTCCCCACTGCGCGCTCTGCTGATAGATTTGc                                                                                         >  2:394268/1‑140 (MQ=255)                                          ttCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTGCGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCt                                                                                       >  2:113996/1‑140 (MQ=255)                                          ttCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTGCGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCt                                                                                       <  1:996936/140‑1 (MQ=255)                                               gggAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTGCGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTa                                                                                  <  1:323157/140‑1 (MQ=255)                                                           gcgcTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTGCGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACa                                                                      >  2:645931/1‑140 (MQ=255)                                                            cgcTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTGCGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAg                                                                     <  2:1159310/140‑1 (MQ=255)                                                            cgcTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTGCGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAg                                                                     <  2:395348/140‑1 (MQ=255)                                                            cgcTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTGCGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATCTGCCTGTCTAGTTGTTTTTACAg                                                                     <  2:181344/140‑1 (MQ=255)                                                             gcTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTGCGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAgg                                                                    <  2:778098/140‑1 (MQ=255)                                                             gcTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTGCGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAgg                                                                    <  1:313196/140‑1 (MQ=255)                                                               ttACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTGCGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAgttg                                                                              <  2:342340/128‑1 (MQ=255)                                                               ttACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTGCGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAgttg                                                                              >  1:342340/1‑128 (MQ=255)                                                               ttACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTGCGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAg                                                                     <  2:255314/137‑1 (MQ=255)                                                               ttACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTGCGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAg                                                                     >  1:255314/1‑137 (MQ=255)                                                                tACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTGCGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGgag                                                                 <  1:837503/140‑1 (MQ=255)                                                                       catGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTGCGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAgatgatga                                                          >  2:739593/1‑140 (MQ=255)                                                                         tGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTGCGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAggg                                                                   <  2:851423/129‑1 (MQ=255)                                                                         tGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTGCGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAggg                                                                   >  1:851423/1‑129 (MQ=255)                                                                         tGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTGCGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGatgatgatg                                                        >  1:1021041/1‑140 (MQ=255)                                                                                  aGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTGCGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTaaaaaa                                               >  1:248747/1‑140 (MQ=255)                                                                                       cgcTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTGCGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGc                                          >  2:1418774/1‑140 (MQ=255)                                                                                       cgcTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTGCGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGc                                          >  1:1231200/1‑140 (MQ=255)                                                                                                 cGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTGCGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAgcgc                                <  1:633698/140‑1 (MQ=255)                                                                                                  ggTGATGGGGCGTTTATTCGTGACGAGGCTAACATTGCGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGcc                               <  1:739593/140‑1 (MQ=255)                                                                                                      aTGGGGCGTTTATTCGTGACGAGGCTAACATTGCGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGcc                           >  1:517516/1‑140 (MQ=255)                                                                                                           gCGTTTATTCGTGACGAGGCTAACATTGCGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGa                      >  2:445928/1‑140 (MQ=255)                                                                                                           gCGTTTATTCGTGACGAGGCTAACATTGCGCTGGGTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGa                      >  2:774131/1‑140 (MQ=255)                                                                                                              tttATTCGTGACGAGGCTAACATTGCGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGc                   <  2:19664/140‑1 (MQ=255)                                                                                                              tttATTCGTGACGAGGCTAACATTGCGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGc                   >  2:88399/1‑140 (MQ=255)                                                                                                                   tCGTGACGAGGCTAACATTGCGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCgtg              <  2:548524/140‑1 (MQ=255)                                                                                                                          gAGGCTAACATTGCGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGa       <  1:113996/140‑1 (MQ=255)                                                                                                                            ggCTAACATTGCGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGaaa     >  2:504726/1‑140 (MQ=255)                                                                                                                             gCTAACATTGCGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAg    >  2:1004824/1‑140 (MQ=255)                                                                                                                               tAACATTGCGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGaa  <  2:832870/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                     GAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAA  >  NC_000913/3254834‑3255099

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑