| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,300,238 | T→C | S91S (AGT→AGC) | yhbQ → | DNA damage response nuclease YhbQ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,300,238 | 0 | T | C | 98.2% | 186.6 / ‑3.5 | 55 | S91S (AGT→AGC) | yhbQ | DNA damage response nuclease YhbQ |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (27/27); minor base G (0/1); total (27/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
CTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGGCGCTTTCAGTGCCCGGCCAGCGGCAACGCAGATCGTG > NC_000913/3300101‑3300377 | cTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCt > 2:234391/1‑140 (MQ=255)cTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCt > 1:451029/1‑140 (MQ=255) gCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCa > 2:1394750/1‑138 (MQ=255) gCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCa < 1:466451/138‑1 (MQ=255) gCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCa > 2:466451/1‑138 (MQ=255) gCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCa < 1:1394750/138‑1 (MQ=255) ggTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAAccc > 2:49870/1‑140 (MQ=255) ggTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAAccc < 2:451029/140‑1 (MQ=255) cgcCAGTGGGCGATCGCTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCgg < 2:240020/132‑1 (MQ=255) cgcCAGTGGGCGATCGCTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCgg > 1:240020/1‑132 (MQ=255) cAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTaaa < 2:1367980/137‑1 (MQ=255) cAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTaaa > 1:1367980/1‑137 (MQ=255) gTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGa < 2:951910/140‑1 (MQ=255) tCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATg > 2:369719/1‑140 (MQ=255) tCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGCATGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATg > 2:1026473/1‑140 (MQ=255) ttCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTaaa < 1:783464/124‑1 (MQ=255) ttCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGGTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTaaa > 2:783464/1‑124 (MQ=255) cGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTaaaa < 1:354467/123‑1 (MQ=255) cGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTaaaa > 2:354467/1‑123 (MQ=255) cGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGt < 1:1084110/140‑1 (MQ=255) gCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTg > 2:359560/1‑140 (MQ=255) gCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATAc > 1:1444514/1‑140 (MQ=255) gCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATAc > 1:812383/1‑140 (MQ=255) gCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATAc > 2:862304/1‑140 (MQ=255) gCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATAc < 1:1026473/140‑1 (MQ=255) aCGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAAc < 1:1290920/140‑1 (MQ=255) ggCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTAGTGATACTCAACCAg < 1:49870/140‑1 (MQ=255) gCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAgg < 2:812383/140‑1 (MQ=255) gAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCg > 2:1178909/1‑140 (MQ=255) aaTATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGGCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCgg < 2:1229106/140‑1 (MQ=255) aaTATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCgg < 2:1444514/140‑1 (MQ=255) aaTATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCgg < 1:1026372/140‑1 (MQ=255) atCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTaa < 1:359560/140‑1 (MQ=255) tCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAAc < 1:234391/140‑1 (MQ=255) cgcgTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACg < 1:1178909/140‑1 (MQ=255) tAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCAtt > 2:830676/1‑140 (MQ=255) aGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCa > 1:462875/1‑140 (MQ=255) tGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGt > 1:1259113/1‑140 (MQ=255) gACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTc < 1:1123782/140‑1 (MQ=255) aCGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGc > 1:932491/1‑140 (MQ=255) cGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCt < 1:601730/140‑1 (MQ=255) aaaaGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACg < 2:315016/140‑1 (MQ=255) aaGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGAt < 2:149502/140‑1 (MQ=255) cTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTgg < 2:118904/140‑1 (MQ=255) gCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGgcg < 2:932491/140‑1 (MQ=255) ggCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGGCGCTTTCa > 1:1012457/1‑140 (MQ=255) cgcAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGGCGCTTTCAGt > 2:124728/1‑140 (MQ=255) gCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGGCGCTTTCAGTGCCCGGCCAg < 2:462875/140‑1 (MQ=255) gCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGGCGCTTTCAGTGCCCGGCCAGCGGCa > 1:1241714/1‑140 (MQ=255) gCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGGCGCTTTCAGTGCCCGGCCAGCGGCa > 1:1041369/1‑140 (MQ=255) ttAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGGAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGGCGCTTTCAGTGCCCGGCCAGCGGCAACGc > 1:973020/1‑140 (MQ=255) ttAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGGCGCTTTCAGTGCCCGGCCAGCGGCAACGc < 1:369719/140‑1 (MQ=255) ttAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGGCGCTTTCAGTGCCCGGCCAGCGGCAACGc > 1:652927/1‑140 (MQ=255) agcagcCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGGCGCTTTCAGTGCCCGGCCAGCGGCAACGCAGa < 2:1012457/140‑1 (MQ=255) gCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGGCGCTTTCAGTGCCCGGcc < 2:810373/125‑1 (MQ=255) gCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGGCGCTTTCAGTGCCCGGcc > 1:810373/1‑125 (MQ=255) tCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGGCGCTTTCAGTTCCCGGCCAGCGGCAACGCAGATCGTg > 2:378820/1‑140 (MQ=255) | CTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGGCGCTTTCAGTGCCCGGCCAGCGGCAACGCAGATCGTG > NC_000913/3300101‑3300377 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |