Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	234,901	T→C	L29P (CTG→CCG)	yafS →	putative S‑adenosyl‑L‑methionine‑dependent methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	234,901	0	T	C	100.0%	282.5 / NA	81	L29P (CTG→CCG)	yafS	putative S‑adenosyl‑L‑methionine‑dependent methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (44/37);  total (44/37)

ACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGAATGCC  >  NC_000913/234768‑235036                                                                                                                                      |                                                                                                                                        tcTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAgcg                                                                                                                                   <  1:955851/139‑1 (MQ=255)  cTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAgcgc                                                                                                                                  <  1:123628/140‑1 (MQ=255)  cTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAgcgc                                                                                                                                  <  1:132476/140‑1 (MQ=255)     ttAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAg                                                                                                                               >  2:1371811/1‑140 (MQ=255)       aaGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCt                                                                                                                             <  1:944068/140‑1 (MQ=255)       aaGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCt                                                                                                                             >  1:174968/1‑140 (MQ=255)       aaGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCt                                                                                                                             >  2:785175/1‑140 (MQ=255)             cATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAgcgc                                                                                                                                  >  2:45543/1‑129 (MQ=255)             cATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAAccc                                                                                                                       >  1:937354/1‑140 (MQ=255)             cATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAAccc                                                                                                                       >  2:68855/1‑140 (MQ=255)             cATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAAccc                                                                                                                       >  1:456284/1‑140 (MQ=255)             cATAGATTACCCCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAgcgc                                                                                                                                  <  1:45543/129‑1 (MQ=255)              aTAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCg                                                                                                                      <  1:1111246/140‑1 (MQ=255)              aTAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCg                                                                                                                      <  1:1371811/140‑1 (MQ=255)                aggttACCTCTCAGTGTGAAACGGGAGGTTGTGATGAACACGGCAAGAGTCACTCAAGCTGTCGTGGCTCATGATGGCTGGGGCGATTTGCCCTGGGGAAAACGTTATCGCAAGGCGCTGGAGCGCAAGATCAACCCGTg                                                                                                                    <  2:816533/137‑1 (MQ=255)                aGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTg                                                                                                                    <  1:226637/140‑1 (MQ=255)                    tACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCt                                                                                                                             <  1:722499/127‑1 (MQ=255)                    tACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCt                                                                                                                             >  2:722499/1‑127 (MQ=255)                    tACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTc                                                                                                                >  2:737031/1‑140 (MQ=255)                    tACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTc                                                                                                                >  1:1171089/1‑140 (MQ=255)                     aCCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCa                                                                                                               >  2:1329571/1‑140 (MQ=255)                     aCCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCa                                                                                                               >  1:127798/1‑140 (MQ=255)                       ctctCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACt                                                                                                             >  1:1035487/1‑140 (MQ=255)                          tcAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTaaa                                                                                                          <  2:456284/140‑1 (MQ=255)                          tcAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTaaa                                                                                                          >  2:1136884/1‑140 (MQ=255)                            aGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAAt                                                                                                        >  1:312492/1‑140 (MQ=255)                              tgtgAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGt                                                                                                      >  1:976723/1‑140 (MQ=255)                              tgtgAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGt                                                                                                      >  2:1177471/1‑140 (MQ=255)                               gtgAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTa                                                                                                     <  2:976723/140‑1 (MQ=255)                               gtgAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTa                                                                                                     <  2:351262/140‑1 (MQ=255)                                tgAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTAt                                                                                                    >  2:921133/1‑140 (MQ=255)                                 gAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTaaa                                                                                                          >  2:1053262/1‑133 (MQ=255)                                 gAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTaaa                                                                                                          <  1:1053262/133‑1 (MQ=255)                                 gAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATg                                                                                                   >  1:1165251/1‑140 (MQ=255)                                   aaCGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGt                                                                                                 <  2:1171089/140‑1 (MQ=255)                                        aaGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACt                                                                                                             >  1:823769/1‑123 (MQ=255)                                        aaGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACt                                                                                                             <  2:823769/123‑1 (MQ=255)                                        aaGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCa                                                                                            >  2:504257/1‑140 (MQ=255)                                        aaGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCa                                                                                            <  1:921133/140‑1 (MQ=255)                                        aaGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCa                                                                                            >  1:48546/1‑140 (MQ=255)                                        aaGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCa                                                                                            >  2:801116/1‑140 (MQ=255)                                              gtgATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCt                                                                                      <  1:737031/140‑1 (MQ=255)                                               tgatgaAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGTCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCa                                                                                            >  2:756534/1‑133 (MQ=255)                                               tgatgaAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGTCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCa                                                                                            <  1:756534/133‑1 (MQ=255)                                               tgatgaAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCtt                                                                                     <  2:174968/140‑1 (MQ=255)                                                   gaAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGa                                                                                 <  1:1177471/140‑1 (MQ=255)                                                           cAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAAt                                                                         >  2:1427942/1‑140 (MQ=255)                                                               agTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTaa                                                                     >  1:1326496/1‑140 (MQ=255)                                                                       aaaCTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGaa                                                             <  2:937354/140‑1 (MQ=255)                                                                       aaaCTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGaa                                                             <  1:1427942/140‑1 (MQ=255)                                                                         aCTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAAt                                                           <  2:1084929/140‑1 (MQ=255)                                                                         aCTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAAt                                                           >  2:203573/1‑140 (MQ=255)                                                                          cTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATc                                                          >  1:197209/1‑140 (MQ=255)                                                                             tCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAAt                                                       >  1:1080167/1‑140 (MQ=255)                                                                             tCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAAt                                                       >  2:1376039/1‑140 (MQ=255)                                                                               gTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTg                                                     >  1:1041733/1‑140 (MQ=255)                                                                                  gCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGa                                                  <  2:1035487/140‑1 (MQ=255)                                                                                       tGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGt                                             >  1:87692/1‑140 (MQ=255)                                                                                          ttGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTgcg                                          <  1:1329571/140‑1 (MQ=255)                                                                                          ttGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTgcg                                          <  1:1451046/140‑1 (MQ=255)                                                                                           tGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTgcgc                                         <  2:1326496/140‑1 (MQ=255)                                                                                           tGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTgcgc                                         <  1:1136884/140‑1 (MQ=255)                                                                                           tGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTgcgc                                         <  2:48546/140‑1 (MQ=255)                                                                                             cTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCgg                                       >  2:139105/1‑140 (MQ=255)                                                                                                gggCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTgcgc                                         >  2:993605/1‑135 (MQ=255)                                                                                                gggCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTgcgc                                         <  1:993605/135‑1 (MQ=255)                                                                                                   cGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTtctc                                 >  2:342898/1‑140 (MQ=255)                                                                                                   cGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTtctc                                 <  2:1122696/140‑1 (MQ=255)                                                                                                   cGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTtctc                                 >  1:468338/1‑140 (MQ=255)                                                                                                        tGCCCTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCaa                            <  1:203573/140‑1 (MQ=255)                                                                                                          cccTGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGt                          <  1:342898/140‑1 (MQ=255)                                                                                                             tGGGGAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGaa                       <  2:468338/140‑1 (MQ=255)                                                                                                               gggAAAGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATg                     >  1:1100006/1‑140 (MQ=255)                                                                                                                  aaaGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGttt                  <  1:1376039/140‑1 (MQ=255)                                                                                                                    aGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCt                >  1:269286/1‑140 (MQ=255)                                                                                                                    aGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCt                >  2:992145/1‑140 (MQ=255)                                                                                                                    aGCTTTATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCt                >  2:1053244/1‑140 (MQ=255)                                                                                                                        ttATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTgcg             <  2:837417/139‑1 (MQ=255)                                                                                                                        ttATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTgcg             >  1:837417/1‑139 (MQ=255)                                                                                                                         tATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGCa           >  2:783401/1‑140 (MQ=255)                                                                                                                         tATCGCAAGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGCa           >  2:1340454/1‑140 (MQ=255)                                                                                                                         tATCGCAAGGCACCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGCa           >  1:778480/1‑140 (MQ=255)                                                                                                                                aGGCGCCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGAATg    <  1:783401/140‑1 (MQ=255)                                                                                                                                  gcgcCGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGAATGcc  <  2:269286/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                        ACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGAATGCC  >  NC_000913/234768‑235036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑