Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,375,991	T→C	F375L (TTT→CTT)	dcuD →	putative transporter DcuD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,375,991	0	T	C	100.0%	239.3 / NA	69	F375L (TTT→CTT)	dcuD	putative transporter DcuD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/33);  total (36/33)

CTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTT  >  NC_000913/3375855‑3376125                                                                                                                                         |                                                                                                                                       cTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTa                                                                                                                                     <  1:993411/140‑1 (MQ=255) attAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTa                                                                                                                                     <  1:221954/139‑1 (MQ=255)           aTTGGCACTGTCGATGCGGTTATCAGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGc                                                                                                                           >  1:299405/1‑140 (MQ=255)           aTTGGCACTGTCGATGCGGTTATCAGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGc                                                                                                                           >  1:976397/1‑140 (MQ=255)           aTTGGCACTGTCGATGCGGTTATCAGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAAAGCGCCGCTTATGTCATTTGc                                                                                                                           <  2:142685/140‑1 (MQ=255)             tGGCACTGTCGATGCGGTTATCAGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCa                                                                                                                         <  2:151765/140‑1 (MQ=255)                  cTGTCGATGCGGTTATCAGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCAGTCtt                                                                                                                    <  2:492825/140‑1 (MQ=255)                     tCGATGCGGTTATCAGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTc                                                                                                                                 <  1:885572/124‑1 (MQ=255)                     tCGATGCGGTTATCAGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTc                                                                                                                                 >  2:885572/1‑124 (MQ=255)                         tGCGGTTATCAGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGa                                                                                                             <  1:1380492/140‑1 (MQ=255)                               tATCAGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCg                                                                                                       >  2:897122/1‑140 (MQ=255)                                  cAGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAg                                                                                                    <  1:1180816/140‑1 (MQ=255)                                   aGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGc                                                                                                   <  1:1343061/140‑1 (MQ=255)                                     gggggCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCACTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAg                                                                                                    <  2:264950/137‑1 (MQ=255)                                     gggggCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCACTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAg                                                                                                    >  1:264950/1‑137 (MQ=255)                                     gggggCGGAGCATTCTGGGCTGGGCGGTATTTGCGTGATGATTATTATGGCGCTGGTCATTTCCCTTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCg                                                                                                 >  2:739672/1‑140 (MQ=255)                                       gggCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGa                                                                                               >  2:881509/1‑140 (MQ=255)                                        ggCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGAc                                                                                              >  2:1003513/1‑140 (MQ=255)                                        ggCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGAc                                                                                              >  2:220105/1‑140 (MQ=255)                                        ggCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGAc                                                                                              >  1:941232/1‑140 (MQ=255)                                         gCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACt                                                                                             <  1:124772/140‑1 (MQ=255)                                          cGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGc                                                                                                   >  2:146448/1‑133 (MQ=255)                                          cGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGc                                                                                                   <  1:146448/133‑1 (MQ=255)                                          cGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTa                                                                                            >  2:1466991/1‑140 (MQ=255)                                           ggAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTAc                                                                                           <  1:915250/140‑1 (MQ=255)                                            gAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACa                                                                                          <  2:733849/140‑1 (MQ=255)                                            gAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACa                                                                                          <  2:941232/140‑1 (MQ=255)                                             aGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACAt                                                                                         >  2:1450372/1‑140 (MQ=255)                                                  tctggtctggGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTAcc                                                                                    <  2:976397/140‑1 (MQ=255)                                                  tctggtctggGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTAcc                                                                                    <  2:299405/140‑1 (MQ=255)                                                    tggtctggGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAg                                                                                  <  1:881509/140‑1 (MQ=255)                                                    tggtctggGCGGCATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAg                                                                                  <  1:1469624/140‑1 (MQ=255)                                                      gtctggGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCg                                                                                >  1:353719/1‑140 (MQ=255)                                                       tctggGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACt                                                                                             >  1:936121/1‑126 (MQ=255)                                                       tctggGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACt                                                                                             <  2:936121/126‑1 (MQ=255)                                                       tctggGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCgg                                                                               <  1:1003513/140‑1 (MQ=255)                                                       tctggGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCgg                                                                               >  1:1130808/1‑140 (MQ=255)                                                           ggCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGc                                                                                 >  1:672596/1‑134 (MQ=255)                                                           ggCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGc                                                                                 >  1:1436732/1‑134 (MQ=255)                                                           ggCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGc                                                                                 <  2:672596/134‑1 (MQ=255)                                                           ggCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGc                                                                                 <  2:1436732/134‑1 (MQ=255)                                                                tATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGa                                                                      >  1:1081031/1‑140 (MQ=255)                                                                          atgatTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGAt                                                            >  1:1128543/1‑140 (MQ=255)                                                                          atgatTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGAt                                                            >  2:187102/1‑140 (MQ=255)                                                                          atgatTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGAt                                                            >  2:581147/1‑140 (MQ=255)                                                                          atgatTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGAt                                                            >  1:589992/1‑140 (MQ=255)                                                                          atgatTATTATGGCACTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGAt                                                            >  2:812085/1‑140 (MQ=255)                                                                                    tGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGcc                                                  >  1:1312651/1‑140 (MQ=255)                                                                                     ggCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCa                                                 >  1:946694/1‑140 (MQ=255)                                                                                     ggCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCa                                                 <  1:1466991/140‑1 (MQ=255)                                                                                       cgcTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCacg                                               >  1:10241/1‑140 (MQ=255)                                                                                       cgcTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCacg                                               >  1:652474/1‑140 (MQ=255)                                                                                       cgcTGGTCATTGCCATTTGAGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCacg                                               >  2:880309/1‑140 (MQ=255)                                                                                         cTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCacgac                                             >  1:1463243/1‑140 (MQ=255)                                                                                            gTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCt                                          >  1:156823/1‑140 (MQ=255)                                                                                            gTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCt                                          >  1:377721/1‑140 (MQ=255)                                                                                                  gCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAgcgcg                                    <  2:652474/140‑1 (MQ=255)                                                                                                         gtgCCATTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGtt                             <  2:1366192/140‑1 (MQ=255)                                                                                                              aTTGTGATGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGcc                        <  1:739672/140‑1 (MQ=255)                                                                                                                    aTGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCg                          >  2:1051904/1‑132 (MQ=255)                                                                                                                    aTGGGCTCTGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCg                          <  1:1051904/132‑1 (MQ=255)                                                                                                                         ctctGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCgg             <  2:10241/140‑1 (MQ=255)                                                                                                                         ctctGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCgg             <  1:581147/140‑1 (MQ=255)                                                                                                                            tGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCggtgg          <  2:353719/140‑1 (MQ=255)                                                                                                                            tGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCggtgg          >  1:247797/1‑140 (MQ=255)                                                                                                                            tGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCggtgg          <  2:1463406/140‑1 (MQ=255)                                                                                                                            tGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCggtgg          >  2:141432/1‑140 (MQ=255)                                                                                                                            tGGCAATGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCggtgg          >  1:1127634/1‑140 (MQ=255)                                                                                                                            tGGCAATGCACCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGGTGCATTTTTCCACGGCGCCAGCGCGCGCGGTTTCGCCGATTCCTGCggggg          >  2:1443476/1‑140 (MQ=255)                                                                                                                                  tGCGCCGCTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCg    <  2:1463243/140‑1 (MQ=255)                                                                                                                                    cgccgcTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGtt  >  2:1464512/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       CTTAACGACGATTGGCACTGTCGATGCGGTTATCAGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTT  >  NC_000913/3375855‑3376125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑