Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,411,037	C→T	A253V (GCA→GTA)	dusB →	tRNA‑dihydrouridine synthase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,411,037	0	C	T	100.0%	329.3 / NA	96	A253V (GCA→GTA)	dusB	tRNA‑dihydrouridine synthase B
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (49/47);  total (49/47)

CTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCAG  >  NC_000913/3410898‑3411164                                                                                                                                            |                                                                                                                                cTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGc                                                                                                                                 >  2:498627/1‑140 (MQ=255)         cAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTa                                                                                                                         <  2:945543/140‑1 (MQ=255)         cAGAGCTGTACTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTCCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTa                                                                                                                         <  2:90108/140‑1 (MQ=255)                 tgCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCgcttg                                                                                                                 <  1:951854/140‑1 (MQ=255)                         tataCAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTgcgc                                                                                                         <  2:357261/140‑1 (MQ=255)                          ataCAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTgcgcg                                                                                                        <  1:211561/140‑1 (MQ=255)                          ataCAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTgcgcg                                                                                                        <  1:1075269/140‑1 (MQ=255)                           taCAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACCCTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTgcgcgc                                                                                                       >  1:758905/1‑140 (MQ=255)                           taCAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTgcgcgc                                                                                                       >  2:1067539/1‑140 (MQ=255)                           taCAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTgcgcgc                                                                                                       >  2:1289492/1‑140 (MQ=255)                           taCAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTgcgcgc                                                                                                       >  1:1100879/1‑140 (MQ=255)                            aCAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACCCTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCa                                                                                                      <  2:758905/140‑1 (MQ=255)                                  gCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCg                                                                                                >  1:1070650/1‑140 (MQ=255)                                  gCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGATGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCg                                                                                                >  1:1416675/1‑140 (MQ=255)                                   cGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCgg                                                                                               >  2:186907/1‑140 (MQ=255)                                   cGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCgg                                                                                               >  1:1110658/1‑140 (MQ=255)                                    ggATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCggg                                                                                              <  1:991402/140‑1 (MQ=255)                                     gATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCa                                                                                                      <  1:1093571/131‑1 (MQ=255)                                     gATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCa                                                                                                      >  2:1093571/1‑131 (MQ=255)                                     gATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGa                                                                                             >  2:180603/1‑140 (MQ=255)                                     gATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGa                                                                                             >  2:821621/1‑140 (MQ=255)                                     gATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGa                                                                                             >  2:227836/1‑140 (MQ=255)                                          ccTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGc                                                                                        >  1:82353/1‑140 (MQ=255)                                          ccTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGc                                                                                        <  1:1289492/140‑1 (MQ=255)                                          ccTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGG‑GTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCa                                                                                       >  1:895455/1‑140 (MQ=255)                                            tgatgaTAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCAt                                                                                      <  1:1044233/140‑1 (MQ=255)                                             gatgatAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATg                                                                                     <  2:1408450/140‑1 (MQ=255)                                                   aGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGaa                                                                                            <  2:1114819/127‑1 (MQ=255)                                                   aGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGaa                                                                                            >  1:1114819/1‑127 (MQ=255)                                                      ccGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATg                                                                            >  2:947426/1‑140 (MQ=255)                                                      ccGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATg                                                                            >  2:634435/1‑140 (MQ=255)                                                        gcggAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCGGGACACTGGGGAGTTGCTGCCCCCGCTTCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGt                                                                          >  2:1121962/1‑140 (MQ=255)                                                        gcgcAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGt                                                                          >  1:1067240/1‑140 (MQ=255)                                                        gcgcAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGt                                                                          <  2:1070650/140‑1 (MQ=255)                                                         cgcAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTc                                                                         >  2:1334482/1‑140 (MQ=255)                                                         cgcAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTc                                                                         <  1:1067539/140‑1 (MQ=255)                                                         cgcAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTc                                                                         >  1:141933/1‑140 (MQ=255)                                                         cgcAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTc                                                                         >  1:717127/1‑140 (MQ=255)                                                         cgcAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTc                                                                         <  2:141933/140‑1 (MQ=255)                                                               tCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCaa                                                                   <  2:1396603/140‑1 (MQ=255)                                                                   ggAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCgg                                                                      >  2:809903/1‑133 (MQ=255)                                                                   ggAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCgg                                                                      <  1:809903/133‑1 (MQ=255)                                                                        aCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTc                                                                         <  1:606480/125‑1 (MQ=255)                                                                        aCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTc                                                                         >  2:606480/1‑125 (MQ=255)                                                                        aCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCaa                                                                   <  1:606416/131‑1 (MQ=255)                                                                        aCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCaa                                                                   >  2:606416/1‑131 (MQ=255)                                                                        aCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTAcc                                                          >  2:1351360/1‑140 (MQ=255)                                                                        aCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTAcc                                                          >  2:1426669/1‑140 (MQ=255)                                                                        aCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTAcc                                                          >  1:990705/1‑140 (MQ=255)                                                                        aCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTAcc                                                          >  2:267516/1‑140 (MQ=255)                                                                        aCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTAcc                                                          >  1:220306/1‑140 (MQ=255)                                                                        aCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTAcc                                                          >  1:1370018/1‑140 (MQ=255)                                                                        aCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTAcc                                                          >  2:901727/1‑140 (MQ=255)                                                                          ccTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGa                                                        >  2:688838/1‑140 (MQ=255)                                                                            tGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAAt                                                      >  1:822460/1‑140 (MQ=255)                                                                             ggATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAAtt                                                     >  1:411049/1‑140 (MQ=255)                                                                              gATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTg                                                    <  1:1040376/140‑1 (MQ=255)                                                                                  tttCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACg                                                >  1:897761/1‑140 (MQ=255)                                                                                   ttCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAggg                                                              <  1:1043754/125‑1 (MQ=255)                                                                                   ttCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAggg                                                              >  2:1043754/1‑125 (MQ=255)                                                                                   ttCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGaa                                                       >  1:1476692/1‑132 (MQ=255)                                                                                   ttCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGaa                                                       <  2:1476692/132‑1 (MQ=255)                                                                                   ttCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGt                                               >  1:1095720/1‑140 (MQ=255)                                                                                    tCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTa                                              >  1:1432055/1‑140 (MQ=255)                                                                                       ggAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAac                                           <  1:227836/140‑1 (MQ=255)                                                                                        gAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGaa                                                       <  1:207656/127‑1 (MQ=255)                                                                                        gAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGaa                                                       >  2:207656/1‑127 (MQ=255)                                                                                         aaaTCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAacac                                         <  2:411049/140‑1 (MQ=255)                                                                                           aTCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGt                                       <  2:220306/140‑1 (MQ=255)                                                                                                gCATTATCTGGACACTGGGG‑GTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTg                                 <  2:895455/140‑1 (MQ=255)                                                                                                 cATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTg                                 <  2:1110658/140‑1 (MQ=255)                                                                                                   ttATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGt                               <  2:717127/140‑1 (MQ=255)                                                                                                   ttATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGt                               <  1:1351360/140‑1 (MQ=255)                                                                                                   ttATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGt                               <  2:572338/140‑1 (MQ=255)                                                                                                   ttATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGt                               <  1:267516/140‑1 (MQ=255)                                                                                                   ttATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGt                               <  1:829123/140‑1 (MQ=255)                                                                                                   ttATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGt                               <  1:634435/140‑1 (MQ=255)                                                                                                   ttATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGt                               <  1:947426/140‑1 (MQ=255)                                                                                                   ttATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGt                               <  2:822460/140‑1 (MQ=255)                                                                                                      tCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTAtc                            >  1:658433/1‑140 (MQ=255)                                                                                                      tCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTAtc                            <  2:658433/140‑1 (MQ=255)                                                                                                          gACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCa                        <  2:990705/140‑1 (MQ=255)                                                                                                           acacTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACg                                        <  2:126128/123‑1 (MQ=255)                                                                                                           acacTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACg                                        >  1:126128/1‑123 (MQ=255)                                                                                                           acacTGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAg                       <  2:1247182/140‑1 (MQ=255)                                                                                                               tGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGt                               >  1:397233/1‑128 (MQ=255)                                                                                                               tGGGGAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGt                               <  2:397233/128‑1 (MQ=255)                                                                                                                ggggAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAaca                  >  2:699491/1‑140 (MQ=255)                                                                                                                ggggAGTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAaca                  <  2:1432055/140‑1 (MQ=255)                                                                                                                     gTTGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTc             <  1:1426669/140‑1 (MQ=255)                                                                                                                      ttGCTGCCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTcc            >  1:296730/1‑140 (MQ=255)                                                                                                                        gctgcCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCaa          <  2:897761/140‑1 (MQ=255)                                                                                                                         ctgcCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGaa                    >  2:588344/1‑129 (MQ=255)                                                                                                                         ctgcCCCCGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGaa                    <  1:588344/129‑1 (MQ=255)                                                                                                                                cGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCAg  >  2:1334757/1‑140 (MQ=255)                                                                                                                                cGCTGCCTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCAg  >  2:208581/1‑140 (MQ=255)                                                                                                                                      cTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCaa          <  1:993981/126‑1 (MQ=255)                                                                                                                                      cTTTGGTAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCaa          >  2:993981/1‑126 (MQ=255)                                                                                                                                            |                                                                                                                                CTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCAG  >  NC_000913/3410898‑3411164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑