Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,495,653	T→C	L548P (CTG→CCG)	nirB →	nitrite reductase catalytic subunit NirB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,495,653	0	T	C	100.0%	256.6 / NA	74	L548P (CTG→CCG)	nirB	nitrite reductase catalytic subunit NirB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/34);  total (40/34)

AAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTGATGGCGGTAGGTCGTATCGCGCGTGAATTTAATCTC  >  NC_000913/3495518‑3495789                                                                                                                                        |                                                                                                                                         aaGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGCTGTGAAGTTTGCAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAg                                                                                                                                      >  2:350018/1‑140 (MQ=255)     cATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGAtt                                                                                                                                  <  2:1250857/140‑1 (MQ=255)      aTTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTc                                                                                                                                 >  2:870614/1‑140 (MQ=255)         aaaaCCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTaa                                                                                                                              >  1:1154018/1‑140 (MQ=255)         aaaaCCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGACCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTaa                                                                                                                              >  2:685807/1‑140 (MQ=255)              cTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACa                                                                                                                         >  1:880268/1‑140 (MQ=255)                tCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAAc                                                                                                                       >  1:1079350/1‑140 (MQ=255)                tCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAAc                                                                                                                       >  1:1165228/1‑140 (MQ=255)                tCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAAc                                                                                                                       >  1:965996/1‑140 (MQ=255)                tCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAAc                                                                                                                       >  1:620025/1‑140 (MQ=255)                 cGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACt                                                                                                                      <  2:880268/140‑1 (MQ=255)                 cGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACt                                                                                                                      >  1:1222294/1‑140 (MQ=255)                  gaagaaCTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACtt                                                                                                                     >  2:995121/1‑140 (MQ=255)                     gaaCTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCt                                                                                                                  <  2:1357786/140‑1 (MQ=255)                     gaaCTGCTGGCGAAACACGGCAAAGGCTACGGCTGTGAAGTTTGCAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCt                                                                                                                  <  1:350018/140‑1 (MQ=255)                         tgctgGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCt                                                                                                              <  1:870614/140‑1 (MQ=255)                           ctgGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTaa                                                                                                            >  1:1434519/1‑140 (MQ=255)                                   acacGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGa                                                                                                    <  2:1154018/140‑1 (MQ=255)                                     acGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGaaa                                                                                                  <  2:170551/140‑1 (MQ=255)                                      cGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAg                                                                                                 <  1:777760/140‑1 (MQ=255)                                          aaaGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACgg                                                                                             <  2:1030023/140‑1 (MQ=255)                                           aaGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGc                                                                                            <  2:1079350/140‑1 (MQ=255)                                            aGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCa                                                                                           >  1:679574/1‑140 (MQ=255)                                             ggCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCAc                                                                                          <  1:329859/140‑1 (MQ=255)                                             ggCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCAc                                                                                          <  1:592912/140‑1 (MQ=255)                                              gCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCAcc                                                                                         <  2:660943/140‑1 (MQ=255)                                                   ggTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTc                                                                                    >  2:237564/1‑140 (MQ=255)                                                       gtgAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTg                                                                                <  2:265205/140‑1 (MQ=255)                                                       gtgAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTg                                                                                <  2:679574/140‑1 (MQ=255)                                                        tgAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCAcc                                                                                         <  2:813125/130‑1 (MQ=255)                                                        tgAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCAcc                                                                                         >  1:813125/1‑130 (MQ=255)                                                        tgAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGa                                                                               >  1:643781/1‑140 (MQ=255)                                                        tgAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGa                                                                               >  1:1033709/1‑140 (MQ=255)                                                               tGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCAcc                                                                                         <  2:1066507/123‑1 (MQ=255)                                                               tGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCAcc                                                                                         >  1:1066507/1‑123 (MQ=255)                                                               tGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCcgcg                                                                        >  2:282442/1‑140 (MQ=255)                                                               tGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCcgcg                                                                        >  2:946108/1‑140 (MQ=255)                                                                  aaaCCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTtc                                                                     <  2:643781/140‑1 (MQ=255)                                                                        accGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCggg                                                               >  2:378696/1‑140 (MQ=255)                                                                         ccGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGc                                                              >  2:499758/1‑140 (MQ=255)                                                                         ccGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGc                                                              >  1:775446/1‑140 (MQ=255)                                                                              ggTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGa                                                         >  1:255214/1‑140 (MQ=255)                                                                               gTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGaa                                                        <  2:1434519/140‑1 (MQ=255)                                                                                ttCGCTGCTGACCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGaaa                                                       <  1:685807/140‑1 (MQ=255)                                                                                 tCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAAt                                                      <  2:620025/140‑1 (MQ=255)                                                                                  cGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATc                                                     <  1:995121/140‑1 (MQ=255)                                                                                  cGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATc                                                     >  1:222183/1‑140 (MQ=255)                                                                                    ctgctgGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCAc                                                   <  2:1033709/140‑1 (MQ=255)                                                                                    ctgctgGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCAc                                                   <  2:775446/140‑1 (MQ=255)                                                                                    ctgctgGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCAc                                                   >  2:1289831/1‑140 (MQ=255)                                                                                     tgctgGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCAcc                                                  >  2:685550/1‑140 (MQ=255)                                                                                     tgctgGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCAc                                                   <  1:205851/139‑1 (MQ=255)                                                                                     tgctgGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCAc                                                   >  2:205851/1‑139 (MQ=255)                                                                                         ggCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCgg                                              <  1:499758/140‑1 (MQ=255)                                                                                              cctGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAggg                                         <  1:282442/140‑1 (MQ=255)                                                                                               ctgctgGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGc                                        >  1:1395183/1‑140 (MQ=255)                                                                                                 gctgGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGaa                                                        <  2:5622/122‑1 (MQ=255)                                                                                                 gctgGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGaa                                                        >  1:5622/1‑122 (MQ=255)                                                                                                 gctgGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTg                                      <  1:1289831/140‑1 (MQ=255)                                                                                                  ctgGAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTGa                                     <  1:946108/140‑1 (MQ=255)                                                                                                    ggAACGAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTGATg                                   >  1:1094130/1‑140 (MQ=255)                                                                                                         gAATACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTGATGGCGGt                              <  2:668414/140‑1 (MQ=255)                                                                                                            tACATTCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTGATGGCGGTAgg                           >  1:909789/1‑140 (MQ=255)                                                                                                                 tCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTGATGGCGGTAGGTCGTa                      >  1:709903/1‑140 (MQ=255)                                                                                                                 tCTGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTGATGGCGGTAGGTCGTa                      >  1:769732/1‑140 (MQ=255)                                                                                                                   tGAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTGATGGCGGTAGGTCGTATc                    >  2:1018546/1‑140 (MQ=255)                                                                                                                    gAAGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTGATGGCGGTAGGTCGTATcg                   >  2:226740/1‑140 (MQ=255)                                                                                                                     aaGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTGATGGCGGTAGGTCGTATcgc                  >  1:198885/1‑140 (MQ=255)                                                                                                                     aaGCCGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTGATGGCGGTAGGTCGTATcgc                  >  2:1212229/1‑140 (MQ=255)                                                                                                                         cGGAACATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTGATGGCGGTAGGTCGTATCGCGCGt              >  2:481067/1‑140 (MQ=255)                                                                                                                             aCATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTGATGGCGGTAGGTCGTATCGCGCGTGAAt          <  2:1290968/140‑1 (MQ=255)                                                                                                                             aCATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTGATGGCGGTAGGTCGTATCGCGCGTGAAt          <  2:769732/140‑1 (MQ=255)                                                                                                                             aCATACTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTGATGGCGGTAGGTCGTATCGCGCGTGAAt          <  1:237564/140‑1 (MQ=255)                                                                                                                                 aCTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTGATGGCGGTAGGTCGTATCGCGCGTGAATTTaa      <  1:1295899/140‑1 (MQ=255)                                                                                                                                  cTCCGCCGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTGATGGCGGTAGGTCGTATCGCGCGTGAATTTAAt     >  1:318319/1‑140 (MQ=255)                                                                                                                                    ccgccgCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTGATGGCGGTAGGTCGTATCGCGCGTGAATTTAAtct   <  2:222183/140‑1 (MQ=255)                                                                                                                                    ccgccgCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTGATGGCGGTAGGTCGTATCGCGCGTGAATTTAAtct   <  1:226740/140‑1 (MQ=255)                                                                                                                                    ccgccgCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTGATGGCGGTAGGTCGTATCGCGCGTGAATTTAAtct   <  1:1018546/140‑1 (MQ=255)                                                                                                                                     cgccgcAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTGATGGCGGTAGGTCGTATCGCGCGTGAATTTAAtctc  >  1:1104377/1‑140 (MQ=255)                                                                                                                                     cgccgcAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTGATGGCGGTAGGTCGTATCGCGCGTGAATTTAAtctc  <  1:1196944/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         AAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTGATGGCGGTAGGTCGTATCGCGCGTGAATTTAATCTC  >  NC_000913/3495518‑3495789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑