Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,540,380	T→C	V73A (GTT→GCT)	feoA →	ferrous iron transport protein A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,540,380	0	T	C	100.0%	237.9 / NA	68	V73A (GTT→GCT)	feoA	ferrous iron transport protein A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/40);  total (28/40)

CTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTAATTGGTAATCCAAATTCTGGCAAGACAACGTTATTTAACCAGCTCACTGGCTCACGTCAGCGTGTAGGTAACTGGGCTGGCG  >  NC_000913/3540245‑3540512                                                                                                                                        |                                                                                                                                     ctctTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTcc                                                                                                                                  <  2:306363/140‑1 (MQ=255) ctctTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTcc                                                                                                                                  <  1:593730/140‑1 (MQ=255)      ggCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTa                                                                                                                             >  2:1134837/1‑140 (MQ=255)        cATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAAt                                                                                                                           <  1:1063972/140‑1 (MQ=255)        cATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAAt                                                                                                                           <  1:1284526/140‑1 (MQ=255)        cATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAAt                                                                                                                           <  1:211130/140‑1 (MQ=255)        cATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAAt                                                                                                                           <  1:366029/140‑1 (MQ=255)          tGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAATAc                                                                                                                         >  2:166409/1‑140 (MQ=255)           gTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAATACg                                                                                                                        >  2:340751/1‑140 (MQ=255)           gTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAATACg                                                                                                                        >  2:1096282/1‑140 (MQ=255)           gTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAATACg                                                                                                                        <  1:604917/140‑1 (MQ=255)            ttACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAATa                                                                                                                          <  1:676049/137‑1 (MQ=255)            ttACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAATa                                                                                                                          >  2:676049/1‑137 (MQ=255)            ttACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAATACgg                                                                                                                       >  2:595651/1‑140 (MQ=255)            ttACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAATACgg                                                                                                                       >  1:434143/1‑140 (MQ=255)            ttACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAATACgg                                                                                                                       >  1:151466/1‑140 (MQ=255)              aCCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAATACGGTg                                                                                                                     >  2:1130460/1‑140 (MQ=255)                 tGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCACTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAATa                                                                                                                          >  1:592817/1‑132 (MQ=255)                 tGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCACTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAATa                                                                                                                          <  2:592817/132‑1 (MQ=255)                      cctcTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAATACg                                                                                                                        >  2:788399/1‑129 (MQ=255)                      cctcTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAATACg                                                                                                                        <  1:788399/129‑1 (MQ=255)                          ttttAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAATACGGTGATAACAACAATg                                                                                                         <  2:121165/140‑1 (MQ=255)                             tAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAATACGGTGATAACAACAATGaaa                                                                                                      <  2:426832/140‑1 (MQ=255)                              aaTGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAATACGGTGATAACAACAATGaaaa                                                                                                     >  1:315012/1‑140 (MQ=255)                                  tggtgCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAATACGGTGATAACAACAATGAAAAAAtt                                                                                                 <  2:434143/140‑1 (MQ=255)                                    gtgCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTaa                                                                                               >  2:674864/1‑140 (MQ=255)                                       cgcgTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCa                                                                                            <  1:340751/140‑1 (MQ=255)                                            cGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGc                                                                                       <  2:1153102/140‑1 (MQ=255)                                            cGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGc                                                                                       >  2:471671/1‑140 (MQ=255)                                               tCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTa                                                                                    >  2:1199682/1‑140 (MQ=255)                                                   cTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTAATTg                                                                                <  2:222769/140‑1 (MQ=255)                                                     cGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTAATTGGt                                                                              <  2:322965/140‑1 (MQ=255)                                                      ggCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTAATTGGTa                                                                             >  1:96559/1‑140 (MQ=255)                                                        cGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTAATTGGTAAt                                                                           >  1:88615/1‑140 (MQ=255)                                                        cGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTAATTGGTAAt                                                                           >  2:349722/1‑140 (MQ=255)                                                         gACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTAATTGGTAATc                                                                          <  1:1130460/140‑1 (MQ=255)                                                           ccccATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTAATTGGTAATCCa                                                                        >  1:918727/1‑140 (MQ=255)                                                             ccATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTAATTGGTAATCCaaa                                                                      >  1:1292502/1‑140 (MQ=255)                                                              cATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTAATTGGTAATCCAAAt                                                                     >  1:625066/1‑140 (MQ=255)                                                              cATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGAGGCTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTAATTGGTAATCCAAAt                                                                     <  1:504244/140‑1 (MQ=255)                                                                   atatCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTAATTGGTAATCCAAATTCTgg                                                                <  2:88615/140‑1 (MQ=255)                                                                        gAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTAATTGGTAATCCAAATTCTGGCAAGa                                                           <  2:720337/140‑1 (MQ=255)                                                                         aaaCCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTAATTGGTAATCCAAATTCTGGCAAGAc                                                          >  2:504378/1‑140 (MQ=255)                                                                          aaCCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTAATTGGTAATCCAAATTCTGGCAAGACa                                                         >  2:525435/1‑140 (MQ=255)                                                                            cccGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTAATTGGTAATCCAAATTCTGGCAAGACAAc                                                       <  1:166409/140‑1 (MQ=255)                                                                            cccGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTAATTGGTAATCCAAATTCTGGCAAGACAAc                                                       <  1:1134837/140‑1 (MQ=255)                                                                            cccGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTAATTGGTAATCCAAATTCTGGCAAGACAAc                                                       <  1:595651/140‑1 (MQ=255)                                                                            cccGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTAATTGGTAATCCAAATTCTGGCAAGACAAc                                                       <  2:315012/140‑1 (MQ=255)                                                                               gtcgtGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTAATTGGTAATCCAAATTCTGGCAAGACAACGtt                                                    <  1:1096282/140‑1 (MQ=255)                                                                                 cgtGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTAATTGGTAATCCAAATTCTGGCAAGACAACGTTAt                                                  <  1:471671/140‑1 (MQ=255)                                                                                   tgtgAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTAATTGGTAATCCAAATTCTGGCAAGACAACGTTAttt                                                <  1:4722/140‑1 (MQ=255)                                                                                      gAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTAATTGGTAATCCAAATTCTGGCAAGACAACGTTATTTAAc                                             <  2:1415114/140‑1 (MQ=255)                                                                                        gCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTAATTGGTAATCCAAATTCTGGCAAGACAACGTTATTTAACCa                                           <  2:151466/140‑1 (MQ=255)                                                                                         ccTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTAATTGGTAATCCAAATTCTGGCAAGACAACGTTATTTAACCAg                                          <  2:752923/140‑1 (MQ=255)                                                                                             gTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTAATTGGTAATCCAAATTCTGGCAAGACAACGTTATTTAACCAGCTCa                                      >  1:841703/1‑140 (MQ=255)                                                                                                   cGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTAATTGGTAATCCAAATTCTGGCAAGACAACGTTATTTAACCAGCTCACTGGCt                                >  1:318779/1‑140 (MQ=255)                                                                                                     cAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTAATTGGTAATCCAAATTCTGGCAAGACAACGTTATTTAACCAGCTCACTGGCTCa                              >  2:1170776/1‑140 (MQ=255)                                                                                                        aaaaGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTAATTGGTAATCCAAATTCTGGCAAGACAACGTTATTTAACCAGCTCACTGGCTCACGt                           <  2:841703/140‑1 (MQ=255)                                                                                                        aaaaGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTAATTGGTAATCCAAATTCTGGCAAGACAACGTTATTTAACCAGCTCACTGGCTCACGt                           <  1:349722/140‑1 (MQ=255)                                                                                                         aaaGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTAATTGGTAATCCAAATTCTGGCAAGACAACGTTATTTAACCAGCTCACTGGCTCACGTc                          <  2:96559/140‑1 (MQ=255)                                                                                                          aaGATCTGGCCTTATTAGAAGTGGAAGCGGCTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTAATTGGTAATCCAAATTCTGGCAAGACAACGTTATTTAACCAGCTCACTGGCTCACGTCa                         <  2:1292502/140‑1 (MQ=255)                                                                                                                      tattaGAAGTGGAAGCGGCTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTAATTGGTAATCCAAATTCTGGCAAGACAACGTTATTTAACCAGCTCACTGGCTCACGTCAGCGTGTAGGTaa             <  1:640467/140‑1 (MQ=255)                                                                                                                      tattaGAAGTGGAAGCGGCTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTAATTGGTAATCCAAATTCTGGCAAGACAACGTTATTTAACCAGCTCACTGGCTCACGTCAGCGTGTAGGTaa             <  2:918727/140‑1 (MQ=255)                                                                                                                       attaGAAGTGGAAGCGGCTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTAATTGGTAATCCAAATTCTGGCAAGACAACGTTATTTAACCAGCTCACTGGCTCACGTCAGCGTGTAGGTAAc            <  1:64592/140‑1 (MQ=255)                                                                                                                        ttaGAAGTGGAAGCGGCTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTAATTGGTAATCCAAATTCTGGCAAGACAACGTTATTTAACCAGCTCACTGGCTCACGTCAGCGTGTAGGTAACt           >  1:579573/1‑140 (MQ=255)                                                                                                                                 gAAGCGGCTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTAATTGGTAATCCAAATTCTGGCAAGACAACGTTATTTAACCAGCTCACTGGCTCACGTCAGCGTGTAGGTAACTgg         <  2:989910/133‑1 (MQ=255)                                                                                                                                 gAAGCGGCTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTAATTGGTAATCCAAATTCTGGCAAGACAACGTTATTTAACCAGCTCACTGGCTCACGTCAGCGTGTAGGTAACTgg         >  1:989910/1‑133 (MQ=255)                                                                                                                                 gAAGCGGCTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTAATTGGTAATCCAAATTCTGGCAAGACAACGTTATTTAACCAGCTCACTGGCTCACGTCAGCGTGTAGGTAACTGGGCTGGCg  <  2:164498/140‑1 (MQ=255)                                                                                                                                 gAAGCGGCTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTAATTGGTAATCCAAATTCTGGCAAGACAACGTTATTTAACCAGCTCACTGGCTCACGTCAGCGTGTAGGTAACTGGGCTGGCg  >  2:299023/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                     CTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTAATTGGTAATCCAAATTCTGGCAAGACAACGTTATTTAACCAGCTCACTGGCTCACGTCAGCGTGTAGGTAACTGGGCTGGCG  >  NC_000913/3540245‑3540512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑