Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,565,382	A→G	Y400Y (TAT→TAC)	glgP ←	glycogen phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,565,382	0	A	G	100.0%	294.7 / NA	85	Y400Y (TAT→TAC)	glgP	glycogen phosphorylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (47/38);  total (47/38)

ACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCTCATCAGCGTGTGGTTAGTG  >  NC_000913/3565245‑3565514                                                                                                                                          |                                                                                                                                     cccctCAGATTAGAGTGCAGTTCGTGTACACCGTTAACTTTGTGCCTCACAACAACCGCCAGCCAGGCCATTCGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCACATCGGTATCGTTCGGGTa                                                                                                                                    <  2:208656/136‑1 (MQ=255)    aTCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACTGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTg                                                                                                                                 <  2:174407/140‑1 (MQ=255)           tAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTg                                                                                                                                 <  1:1080398/133‑1 (MQ=255)           tAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTg                                                                                                                                 >  2:1080398/1‑133 (MQ=255)                tGCAGTTCCGATACACCGTTAACTTTGTGGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGt                                                                                                                     <  2:1067014/140‑1 (MQ=255)                  cAGTTCCGATACACCGTTAACTTTGTGGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGttt                                                                                                                   >  2:191689/1‑140 (MQ=255)                  cAGTTCCGATACACCGTTAACTTTGTGGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGttt                                                                                                                   >  2:667776/1‑140 (MQ=255)                   aGTTCCGATACACCGTTAACTTTGTGGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCa                                                                                                                         >  2:195846/1‑133 (MQ=255)                   aGTTCCGATACACCGTTAACTTTGTGGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCa                                                                                                                         <  1:195846/133‑1 (MQ=255)                   aGTTCCGATACACCGTTAACTTTGTGGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGtttt                                                                                                                  >  2:848169/1‑140 (MQ=255)                   aGTTCCGATACACCGTTAACTTTGTGGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGtttt                                                                                                                  >  1:1274975/1‑140 (MQ=255)                     ttCCGATACACCGTTAACTTTGTGGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCa                                                                                                                >  2:646893/1‑140 (MQ=255)                       ccGATACACCGTTAACTTTGTGGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAgg                                                                                                              >  1:535782/1‑140 (MQ=255)                          aTACACCGTTAACTTTGTGGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGaaa                                                                                                           <  1:312491/140‑1 (MQ=255)                                  ttAACTTTGTGGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGtt                                                                                                   >  2:567412/1‑140 (MQ=255)                                  ttAACTTTGTGGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGtt                                                                                                   <  1:567412/140‑1 (MQ=255)                                  ttAACTTTGTGGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGtt                                                                                                   >  1:1326914/1‑140 (MQ=255)                                   tAACTTTGTGGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGGGACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTg                                                                                                  >  2:1018490/1‑140 (MQ=255)                                     aCTTTGTGGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGAt                                                                                                <  1:411294/140‑1 (MQ=255)                                     aCTTTGTGGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGAt                                                                                                >  2:65190/1‑140 (MQ=255)                                         tgtgGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCa                                                                                            >  2:553759/1‑140 (MQ=255)                                          gtgGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCaa                                                                                           <  2:1351045/140‑1 (MQ=255)                                          gtgGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCaa                                                                                           >  2:894430/1‑140 (MQ=255)                                          gtgGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCaa                                                                                           >  2:278740/1‑140 (MQ=255)                                            ggCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAg                                                                                         >  2:230393/1‑140 (MQ=255)                                             gCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAga                                                                                        <  2:454984/140‑1 (MQ=255)                                              cTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAgat                                                                                       <  1:191689/140‑1 (MQ=255)                                               tCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAgatg                                                                                      >  1:940940/1‑140 (MQ=255)                                                 acaacaACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCaa                                                                                           >  1:838325/1‑133 (MQ=255)                                                 acaacaACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCaa                                                                                           <  2:838325/133‑1 (MQ=255)                                                 acaacaACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAgatgat                                                                                    <  1:553759/140‑1 (MQ=255)                                                 acaacaACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAgatgat                                                                                    >  1:712018/1‑140 (MQ=255)                                                       acCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTAGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAg                                                                              >  2:1254518/1‑140 (MQ=255)                                                             agccagGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGAcg                                                                        >  2:378876/1‑140 (MQ=255)                                                             agccagGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGAcg                                                                        >  1:493572/1‑140 (MQ=255)                                                               ccagGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGAcgcg                                                                      >  2:1379846/1‑140 (MQ=255)                                                                  ggCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCa                                                                   <  2:774658/140‑1 (MQ=255)                                                                   gCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAg                                                                  >  1:222230/1‑140 (MQ=255)                                                                     cATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGaa                                                                <  1:480300/140‑1 (MQ=255)                                                                         cGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAAtttt                                                            <  2:1274975/140‑1 (MQ=255)                                                                         cGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAAtttt                                                            >  1:1036536/1‑140 (MQ=255)                                                                          gCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTa                                                           <  2:222230/140‑1 (MQ=255)                                                                             cacGGCGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGGCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCATAATTGTAccc                                                        <  2:231609/140‑1 (MQ=255)                                                                             cacGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTAccc                                                        <  1:606350/140‑1 (MQ=255)                                                                             cacGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTAccc                                                        <  1:65190/140‑1 (MQ=255)                                                                             cacGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGGCACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTAccc                                                        >  2:1317165/1‑140 (MQ=255)                                                                              acgacgACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTAGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCa                                                       <  1:1254518/140‑1 (MQ=255)                                                                                        ttGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAAc                                             >  2:815901/1‑140 (MQ=255)                                                                                               catcaATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAg                                      <  1:894430/140‑1 (MQ=255)                                                                                               catcaATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAg                                      >  2:209140/1‑140 (MQ=255)                                                                                                atcaATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAgg                                     >  2:691937/1‑140 (MQ=255)                                                                                                    aTGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTc                                 <  1:968660/140‑1 (MQ=255)                                                                                                     tGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTcc                                >  1:1374881/1‑140 (MQ=255)                                                                                                          gACGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGTATTTTACCCAGCATATCAACCGGCCAGGTTTCCAgcgc                           >  2:1237400/1‑140 (MQ=255)                                                                                                          gACGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAgcgc                           >  2:936446/1‑140 (MQ=255)                                                                                                          gACGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCAAGGTTTCCAgcg                            >  2:150920/1‑139 (MQ=255)                                                                                                          gACGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCAAGGTTTCCAgcg                            <  1:150920/139‑1 (MQ=255)                                                                                                           aCGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAg                                      >  1:1086627/1‑128 (MQ=255)                                                                                                           aCGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAg                                      <  2:1086627/128‑1 (MQ=255)                                                                                                           aCGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGcc                          >  1:1128564/1‑140 (MQ=255)                                                                                                           aCGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGcc                          <  1:562716/140‑1 (MQ=255)                                                                                                           aCGCCCGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGcc                          <  1:201237/140‑1 (MQ=255)                                                                                                                cGTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCt                     <  1:646893/140‑1 (MQ=255)                                                                                                                 gTCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCtc                    >  2:669959/1‑140 (MQ=255)                                                                                                                  tCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCtca                   >  1:242690/1‑140 (MQ=255)                                                                                                                  tCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCtca                   >  1:988738/1‑140 (MQ=255)                                                                                                                  tCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCtca                   >  2:50215/1‑140 (MQ=255)                                                                                                                  tCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCtca                   >  2:478249/1‑140 (MQ=255)                                                                                                                  tCCCAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCtca                   <  1:669959/140‑1 (MQ=255)                                                                                                                   cccAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCtcat                  <  2:1036536/140‑1 (MQ=255)                                                                                                                    ccAGCAGATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCtcatc                 <  1:478249/140‑1 (MQ=255)                                                                                                                        cagATCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCTCATCAGCg             <  1:230393/140‑1 (MQ=255)                                                                                                                           aTCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCTCATCAGCgtgt          >  2:877960/1‑140 (MQ=255)                                                                                                                           aTCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCTCATCAGCgtgt          >  1:74454/1‑140 (MQ=255)                                                                                                                            tCGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCTCATCAGCGtgtg         <  1:473477/140‑1 (MQ=255)                                                                                                                             cGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTTACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCTCATCAGCGTGTgg        >  1:42985/1‑140 (MQ=255)                                                                                                                             cGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCTCATCAGCGTGTgg        >  2:266937/1‑140 (MQ=255)                                                                                                                             cGGTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCTCATCAGCGTGTgg        >  1:871009/1‑140 (MQ=255)                                                                                                                              ggTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCTCATCAGCGTGTGGt       <  1:1379846/140‑1 (MQ=255)                                                                                                                              ggTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCTCATCAGCGTGTGGt       <  1:815901/140‑1 (MQ=255)                                                                                                                              ggTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCTCATCAGCGTGTGGt       <  2:1128564/140‑1 (MQ=255)                                                                                                                              ggTATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCTCATCAGCGTGTGGt       <  1:667776/140‑1 (MQ=255)                                                                                                                                tATCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCTCATCAGCGTGTGGTTa     <  1:848169/140‑1 (MQ=255)                                                                                                                                  tCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCTCATCAGCGTGTGGTTAgt   >  1:1031920/1‑140 (MQ=255)                                                                                                                                  tCGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCTCATCAGCGTGTGGTTAgt   >  2:407649/1‑140 (MQ=255)                                                                                                                                   cGTTCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTTACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCTCATCAGCGTGTGGTTAgtg  <  2:42985/140‑1 (MQ=255)                                                                                                                                     ttCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCTCATCAGCGTGTGGt       <  2:779160/133‑1 (MQ=255)                                                                                                                                     ttCGGGTACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCTCATCAGCGTGTGGt       >  1:779160/1‑133 (MQ=255)                                                                                                                                          |                                                                                                                                     ACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCTCATCAGCGTGTGGTTAGTG  >  NC_000913/3565245‑3565514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑