Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,591,352	G→A	A325V (GCG→GTG)	ugpB ←	sn‑glycerol 3‑phosphate ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,591,352	0	G	A	100.0%	144.2 / NA	45	A325V (GCG→GTG)	ugpB	sn‑glycerol 3‑phosphate ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/29);  total (16/29)

TATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCGCCACACCGGTATACGTTTCTTTATCTTTACCCTGCATCACCCACAGGCTGGCTCCGCCGATAATGGCGTTTTGTGGCGCATCTTTCGCATCGGCGTCGTAAGGCATCATGCCTACGC  >  NC_000913/3591215‑3591469                                                                                                                                          |                                                                                                                      tATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATACGCTGC‑TTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACca                                                                                                                    <  2:1407289/140‑1 (MQ=255)  aTCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACca                                                                                                                    <  1:1240403/140‑1 (MQ=255)  aTCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACca                                                                                                                    <  2:454304/140‑1 (MQ=255)  aTCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCG‑TTTCTGGCTTCGCGAGGAAATCGAGGAACTTcaccac                                                                                                                   <  2:1449387/140‑1 (MQ=255)   tCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTcaccac                                                                                                                   >  1:1253644/1‑140 (MQ=255)   tCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGATTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTcaccac                                                                                                                   >  1:306132/1‑140 (MQ=255)     cGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCacac                                                                                                                 <  2:14006/140‑1 (MQ=255)       ccccTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCg                                                                                                               <  1:4060/140‑1 (MQ=255)        cccTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACcaca                                                                                                                  <  2:157223/136‑1 (MQ=255)        cccTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACcaca                                                                                                                  >  1:157223/1‑136 (MQ=255)        cccTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCgg                                                                                                              >  1:702145/1‑140 (MQ=255)         ccTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGt                                                                                                             >  1:1187173/1‑140 (MQ=255)           tGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACcaca                                                                                                                  >  2:81660/1‑133 (MQ=255)           tGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACcaca                                                                                                                  <  1:81660/133‑1 (MQ=255)           tGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGtat                                                                                                           >  1:851725/1‑140 (MQ=255)                  ttCTCATAAAAGCCCTGCTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTtc                                                                                                    >  1:241751/1‑140 (MQ=255)                    ctcATAAAAGCCCTGCTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTtctt                                                                                                  <  2:689270/140‑1 (MQ=255)                    ctcATAAAAGCCCTGCTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTtctt                                                                                                  <  2:540992/140‑1 (MQ=255)                         aaaaGCCCTGCTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTtctttatct                                                                                             <  2:103724/140‑1 (MQ=255)                              cccTGCTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTAcc                                                                                        <  2:1181724/140‑1 (MQ=255)                                cTGCTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTACCCt                                                                                      <  2:654199/140‑1 (MQ=255)                                 tGCTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTACCCTg                                                                                     >  1:338733/1‑140 (MQ=255)                                   cTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTACCCTGCa                                                                                   <  2:128381/140‑1 (MQ=255)                                   cTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTACCCTGCa                                                                                   >  2:153099/1‑140 (MQ=255)                                    tCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTACCCTGCAt                                                                                  <  2:375503/140‑1 (MQ=255)                                        gggTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTACCCTGCATCAcc                                                                              <  2:851725/140‑1 (MQ=255)                                          gtcaggtcaTACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTACCCTGCATCACCca                                                                            <  1:488665/140‑1 (MQ=255)                                             aggtcaTACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGCAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTACCCTGCa                                                                                   <  2:1469044/130‑1 (MQ=255)                                             aggtcaTACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTAcc                                                                                        >  1:313445/1‑125 (MQ=255)                                             aggtcaTACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTAcc                                                                                        <  2:313445/125‑1 (MQ=255)                                             aggtcaTACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTACCCTGCa                                                                                   >  1:1469044/1‑130 (MQ=255)                                                      gctgctTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACCCCGGTATACGTTTCTTTATCTTTACCCTGCATCACCCACAGGCTGGCTcc                                                                <  2:80577/140‑1 (MQ=255)                                                         gctTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTACCCTGCATCACCCACAGGCTGGCTccgcc                                                             <  2:1420370/140‑1 (MQ=255)                                                         gctTCGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTACCCTGCATCACCCACAGGCTGGCTccgcc                                                             >  1:262321/1‑140 (MQ=255)                                                          ctTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTACCCTGCATCACCCACAGGCTGGCTccgccg                                                            >  2:92183/1‑140 (MQ=255)                                                          ctTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTACCCTGCATCACCCACAGGCTGGCTccgccg                                                            >  1:494195/1‑140 (MQ=255)                                                                 gATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTACCCTGCATCACCCACAGGCTGGCTCCGCCGATAATgg                                                     <  1:1368841/140‑1 (MQ=255)                                                                         gATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTACCCTGCATCACCCACAGGCTGGCTCCGCCGATAATGGCGTTTtgt                                             >  1:1303473/1‑140 (MQ=255)                                                                                         aTGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTACCCTGCATCACCCACAGGCTGGCTCCGCCGATAATGGCGTTTTGTGGCGCATCTTTCGCAt                             <  2:1303473/140‑1 (MQ=255)                                                                                            ccACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTACCCTGCATCACCCACAGGCTGGCTCCGCCGATAATGGCGTTTTGTGGCGCATCTTTCGCATCgg                          <  2:262321/140‑1 (MQ=255)                                                                                             cACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACC‑GTATACGTTTCTTTATCTTTCCCCTGCATCACCCACAGGCTGGCTCCGCCGATAATGGCGTTTTGTGGCGCATCTTTCGCATCGGcg                        <  2:315015/140‑1 (MQ=255)                                                                                                    cggCGTTTTCTGGTTTCTAGAGGAAACCGGGTACCTTCGCCACGCCGGTATACGTTTATTTCTCTTAACCCTTCCACGCCCACGGTCTGGATCCGCCGATAATGGCGTTTTGTGGCGCATCTTTCGCATCGGCGTCGTaa                  <  2:306132/138‑1 (MQ=255)                                                                                                    cAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTACCCTGCATCACCCACAGGCTGGCTCCGCCGATAATGGCGTTTTGTGGCGCATCTTTCGCATCGGCGTCGTaa                  <  2:1253644/140‑1 (MQ=255)                                                                                                     aGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTACCCTGCATCACCCACAGGCTGGCTCCGCCGATAATGGCGTTTTGTGGCGCATCTTTCGCATCGGCGTCGTAAg                 <  1:153099/140‑1 (MQ=255)                                                                                                       cGTTTTCTGGCTTCGCGAGGAAATCGAGGACCTTCACCACACCGGTATACGTTTCTTTATCTTTACCCTGCATCACCCACAGGCTGGCTCCGCCGATAATGGCGTTTTGTGGCGCATCTTTCGCATCGGCGTCGTaa                  <  2:1249882/137‑1 (MQ=255)                                                                                                       cGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTACCCTGCATCACCCACAGGCTGGCTCCGCCGATAATGGCGTTTTGTGGCGCATCTTTCGCATCGGCGTCGTaa                  >  1:1249882/1‑137 (MQ=255)                                                                                                         ttttCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTCCCCTGCATCACCCACAGGCTGGCTCCGCCGATAATGGCGTTTTGTGGCGCATCTTTCGCATCGGCGTCGTAAGGcat             <  2:186665/140‑1 (MQ=255)                                                                                                          tttCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTACCCTGCATCACCCACAGGCTGGCTCCGCCGATAATGGCGTTTTGTGGCGCATCTTTCGCATCGGc                         >  1:682665/1‑127 (MQ=255)                                                                                                          tttCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTACCCTGCATCACCCACAGGCTGGCTCCGCCGATAATGGCGTTTTGTGGCGCATCTTTCGCATCGGc                         <  2:682665/127‑1 (MQ=255)                                                                                                                 cTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTACCCTGCATCACCCACAGGCTGGCTCCGCCGATAATGGCGTTTTGTGGCGCATCTTTCGCATCGGCGTCGTAAGGCATCATGCCTa     >  1:525653/1‑140 (MQ=255)                                                                                                                    cgcgAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTACCCTGCATCACCCACAGGCTGGCTCCGCCGATAATGGCGTTTTGTGGCGCATCTTTCGCATCGGCGTCGTAAGGCATCATGCCTACGc  <  1:388970/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                      TATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCGCCACACCGGTATACGTTTCTTTATCTTTACCCTGCATCACCCACAGGCTGGCTCCGCCGATAATGGCGTTTTGTGGCGCATCTTTCGCATCGGCGTCGTAAGGCATCATGCCTACGC  >  NC_000913/3591215‑3591469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑