Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,603,835	T→C	I137V (ATT→GTT)	ftsY ←	signal recognition particle receptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,603,835	0	T	C	100.0%	145.4 / NA	43	I137V (ATT→GTT)	ftsY	signal recognition particle receptor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/23);  total (20/23)

TTCTTCCACCGGCTGCTCTTCTTCTGCCGGAGGAACCACCATCACCGCCTCTTCTGCCGCTTCGGCAGCCAGCGCCGTTTCCAGCTCTTCGTCGGTAATTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTCGACAATCTCTACGGTTTCCGCTTCAG‑CCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGTTCGATAGCCACTGGCTCCGGCGTTTCTTCTACGACCGGTTCCGGCTGTGCA  >  NC_000913/3603698‑3603971                                                                                                                                          |                                                                                                                                          ttcttcCACCGGCTGCTCTTCTTCTGCCGGAGGAACCACCATCACCGCCTCTTCTGCCGCTTCGGCAGCCAGCGCCGTTTCCAGCTCTTCGTCGGTAATTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTCGACAAcct                                                                                                                                         >  1:647056/1‑140 (MQ=255)  tcttcCACCGGCTGCTCTTCTTCTGCCGGAGGAACCACCATCACCGCCTCTTCTGCCGCTTCGGCAGCCAGCGCCGTTTCCAGCTCTTCGTCGGTAATTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTCGACAAcctc                                                                                                                                        >  1:544365/1‑140 (MQ=255)    tccccccGGCTGCTTTTCTTCTGCCGGAGGACCCCCCACCACCGCCTCTTCTGCCGCTTCGGCAGCCAGCGCCGTTTCCAGCTCTTCGTCGGTAATTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTCGACAACCTCTa                                                                                                                                      <  2:1241577/135‑1 (MQ=255)      cccccGGCTGCTCTTCTTCTGCCGGAGGACCCCCCATCACCGCCTCTTCTGCCGCTTCGGCAGCCAGCGCCGTTTCCAGCTCTTCGTCGGTAATTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTCGACAACCTCTACg                                                                                                                                    <  2:544365/137‑1 (MQ=255)          cGGCTGCTCTTCTTCTGCCGGAGGAACCACCATCACCGCCTTTTCTGCCGCTTCGGCAGCCAGCGCCGTTTCCAGCTCTTCGTCGGTAATTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTCGACAACCTCTACGGttt                                                                                                                                <  2:670815/140‑1 (MQ=255)            gctgctCTTCTTCTGCCGGAGGAACCACCATCACCGCCTCTTCTGCCGCTTCGGCAGCCAGCGCCGTTTCCAGCTCTTCGTCGGTAATTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTCGACAACCTCTACGGTTTcc                                                                                                                              >  1:1043597/1‑140 (MQ=255)            gctgctCTTCTTCTGCCGGAGGAACCACCATCACCGCCTCTTCTGCCGCTTCGGCAGCCAGCGCCGTTTCCAGCTCTTCGTCGGTAATTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTCGACAACCTCTACGGTTTcc                                                                                                                              >  1:216051/1‑140 (MQ=255)            gctgctCTTCTTCTGCCGGAGGAACCACCATCACCGCCTCTTCTGCCGCTTCGGCAGCCAGCGCCGTTTCCAGCTCTTCGTCGGTAATTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTCGACAACCTCTACGGTTTcc                                                                                                                              >  2:971809/1‑140 (MQ=255)              tgctCTTCTTCTGCCGGAGGAACCACCATCACCGCCTCTTCTGCCGCTTCGGCAGCCAGCGCCGTTTCCAGCTCTTCGTCGGTAATTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTCGACAACCTCTACGGTTTCCGc                                                                                                                            >  2:421304/1‑140 (MQ=255)                ctctTCTTCTGCCGGAGGAACCACCATCACCGCCTCTTCTGCCGCTTCGGCAGCCAGCGCCGTTTCCAGCTCTTCGTCGGTAATTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTCGACAACCTCTACGGTTTCCGCtt                                                                                                                          >  2:1103369/1‑140 (MQ=255)                  ttttttcgGCGGGAGAACCCCCCACCCCCGCCTCTTCTGCCGCTTCGGCAGCCAGCGCCGTTTCCAGCTCTTCGTCGGTAATTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTCGACAACCTCTACGGTTTCCGCTTCa                                                                                                                        <  2:216051/136‑1 (MQ=255)                     tttctGCGGGAGAACCCCCCATCCCCGCCTCTTCTGCCGCTTCGGCAGCCAGCGCCGTTTCCAGCTCTTCGTCGGTAATTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTCGACAACCTCTACGGTTTCCGCTTCAG‑cc                                                                                                                    <  2:1043597/139‑1 (MQ=255)                          gcgggaggaCCCACCTCCCCCGCCTCTTCTGCCGCTTCGGCAGCCAGCGCCGTTTCCAGCTCTTCGTCGGTAATTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTCGACAACCTCTACGGTTTCCGCTTCAG‑CCTGCCa                                                                                                               <  2:982972/137‑1 (MQ=255)                           ccGGAGGAACCACCATCACCGCCTCTTCTGCCGCTTCGGCAGCCAGCGCCGTTTCCAGCTCTTCGTCGGTAATTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTCGACAACCTCTACGGTTTCCGCTTCAG‑CCTGCCAc                                                                                                              >  2:280950/1‑140 (MQ=255)                                      accaTCACCGCCTCTTCTGCCGCTTCGGCAGCCAGCGCCGTTTCCAGCTCTTCGTCGGTAATTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTCGACAACCTCTACGGTTTCCGCTTCAG‑CCTGCCACTCTTCTGGCGa                                                                                                   <  1:329789/140‑1 (MQ=255)                                           cACCGCCTCTTCTGCCGCTTCGGCAGCCAGCGCCGTTTCCAGCTCTTCGTCGGTAATTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTCGACAACCTCTACGGTTTCCGCTTCAG‑CCTGCCACTCTTCTGGCGAAACCg                                                                                              >  1:821218/1‑140 (MQ=255)                                               gCCTCTTCTGCCGCTTCGGCAGCCAGCGCCGTTTCCAGCTCTTCGTCGGTAATTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTCGACAACCTCTACGGTTTCCGCTTCAG‑CCTGCCACTCTTCTGGCGAAACCGCTTc                                                                                          >  1:84144/1‑140 (MQ=255)                                               gCCTCTTCTGCCGCTTCGGCAGCCAGCGCCGTTTCCAGCTCTTCGTCGGTAATTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTCGACAACCTCTACGGTTTCCGCTTCAG‑CCTGCCACTCTTCTGGCGAAACCGCTTc                                                                                          >  1:1443778/1‑140 (MQ=255)                                                ccTCTTCTGCCGCTTCGGCAGCCAGCGCCGTTTCCAGCTCTTCGTCGGTAATTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTCGACAACCTCTACGGTTTCCGCTTCAG‑CCTGCCACTCTTCTGGc                                                                                                     <  1:847887/128‑1 (MQ=255)                                                ccTCTTCTGCCGCTTCGGCAGCCAGCGCCGTTTCCAGCTCTTCGTCGGTAATTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTCGACAACCTCTACGGTTTCCGCTTCAG‑CCTGCCACTCTTCTGGc                                                                                                     >  2:847887/1‑128 (MQ=255)                                                    ttctGCCGCTTCGGCAGCCAGCGCCGTTTCCAGCTCTTCGTCGGTAATTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTCGACAACCTCTACGGTTTCCGCTTCAG‑CCTGCCACTCTTCTGGCGAAACCGCtt                                                                                           <  2:633643/134‑1 (MQ=255)                                                    ttctGCCGCTTCGGCAGCCAGCGCCGTTTCCAGCTCTTCGTCGGTAATTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTCGACAACCTCTACGGTTTCCGCTTCAG‑CCTGCCACTCTTCTGGCGAAACCGCtt                                                                                           >  1:633643/1‑134 (MQ=255)                                                            cTTCGGCAGCCAGCGCCGTTTCCAGCTCTTCGTCGGTAATTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTCGACAACCTCTACGGTTTCCGCTTCAG‑CCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGa                                                                                  >  1:354692/1‑135 (MQ=255)                                                            cTTCGGCAGCCAGCGCCGTTTCCAGCTCTTCGTCGGTAATTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTCGACAACCTCTACGGTTTCCGCTTCAG‑CCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGa                                                                                  <  2:354692/135‑1 (MQ=255)                                                              tCGGCAGCCAGCGCCGTTTCCAGCTCTTCGTCGGTAATTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTCGACAACCTCTACGGTTTCCGCTTCAG‑CCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTc                                                                           >  2:1056367/1‑140 (MQ=255)                                                              tCGGCAGCCAGCGCCGTTTCCAGCTCTTCGTCGGTAATTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTCGACAACCTCTACGGTTTCCGCTTCAG‑CCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTc                                                                           >  1:290839/1‑140 (MQ=255)                                                                          gcCGTTTCCAGCTCTTCGTCGGTAATTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTCGACAACCTCTACGGTTTCCGCTTCAG‑CCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAg                                                               <  2:276562/140‑1 (MQ=255)                                                                           ccGTTTCCAGCTCTTCGTCGGTAATTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTCGACAACCTCTACGGTTTCCGCTTCAG‑CCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGc                                                              <  2:1443778/140‑1 (MQ=255)                                                                              tttCCAGCTCTTCGTCGGTAATTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTCGACAACCTCTACGGTTTCCGCTTCAG‑CCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGctct                                                           <  2:635073/140‑1 (MQ=255)                                                                                tCCAGCTCTTCGTCGGTAATTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTCGACAACCTCTACGGTTTCCGCTTCAG‑CCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTc                                                         <  1:1103369/140‑1 (MQ=255)                                                                                   aGCTCTTCGTCGGTAATTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTCGACAACCTCTACGGTTTCCGCTTCAG‑CCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACg                                                      <  1:280950/140‑1 (MQ=255)                                                                                        ttCGTCGGTAATTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTCGACAACCTCTACGGTTTCCGCTTCAG‑CCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGTTCGa                                                 <  2:290839/140‑1 (MQ=255)                                                                                          cgtcgGTAATTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTCGACAACCTCTACGGTTTCCGCTTCAG‑CCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGTTCGAt                                                <  2:119431/139‑1 (MQ=255)                                                                                          cgtcgGTAATTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTCGACAACCTCTACGGTTTCCGCTTCAG‑CCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGTTCGAt                                                >  1:119431/1‑139 (MQ=255)                                                                                                  aTTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTCGACAACCTCTACGGTTTCCGCTTCAGCCCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGTTCGATAGCCACTg                                        >  1:644014/1‑140 (MQ=255)                                                                                                         ttttAGCCGCTTCTTCTTCCGCCGCTTCGACAACCTCTCCGGTTTCCGCTTCAG‑CCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGTTCGATAGCCACTGGCTCCGGc                                <  2:1405614/139‑1 (MQ=255)                                                                                                            tAGCCGCTTCTTCTTCCGCCGCTTCGACAACCTCTACGGTTTCCGCTTCAG‑CCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGTTCGATAGCCACTGGCTCCGGCGtt                             <  2:821218/140‑1 (MQ=255)                                                                                                              gCCGCTTCTTCTTCCGCCGCTTCGACAACCTCTACGGTTTCCGCTTCAG‑CCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGTTCGATAGCCACTGGCTCCGGCGTttc                           >  2:910104/1‑140 (MQ=255)                                                                                                                  cttcttcttCCGCCGCTTCGACAACCTCTACGGTTTCCGCTTCAG‑CCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGTTCGATAGCCACTGGCTCCGGCGTTtcttct                       <  1:971809/140‑1 (MQ=255)                                                                                                                             gccgcTTCGACAACCTCTACGGTTTCCGCTTCAG‑CCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGTTCGATAGCCACTGGCTCCGGCGTTTCTTCTACGACCGGTTc            >  2:218462/1‑140 (MQ=255)                                                                                                                              ccgcTTCGACAACCTCTACGGTTTCCGCTTCAG‑CCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGTTCGATAGCCACTGGCTCCGGCGTTTCTTCTACGAc                  <  1:839857/133‑1 (MQ=255)                                                                                                                              ccgcTTCGACAACCTCTACGGTTTCCGCTTCAG‑CCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGTTCGATAGCCACTGGCTCCGGCGTTTCTTCTACGAc                  >  2:839857/1‑133 (MQ=255)                                                                                                                                gcTTCGACAACCTCTACGGTTTCCGCTTCAG‑CCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGTTCGAAAGCCACTGGCTCCGGCGTTTCTTCTACGACCGGTTCCgg         <  2:84144/140‑1 (MQ=255)                                                                                                                                 cTTCGACAACCTCTACGGTTTCCGCTTCAG‑CCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGTTCGATAGCCACTGGCTCCGGCGTTTCTTCTACGACCGGTTCCGGc        >  2:1294905/1‑140 (MQ=255)                                                                                                                                       cAACCTCTACGGTTTCCGCTTCAG‑CCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGTTCGATAGCCACTGGCTCCGGCGTTTCTTCTACGACCGGTTCCGGCTGTGCa  <  1:910104/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                          TTCTTCCACCGGCTGCTCTTCTTCTGCCGGAGGAACCACCATCACCGCCTCTTCTGCCGCTTCGGCAGCCAGCGCCGTTTCCAGCTCTTCGTCGGTAATTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTCGACAATCTCTACGGTTTCCGCTTCAG‑CCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGTTCGATAGCCACTGGCTCCGGCGTTTCTTCTACGACCGGTTCCGGCTGTGCA  >  NC_000913/3603698‑3603971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑